Restriction map for
pF1KA1714
(Circular) (Six-base) MAPSORT of: pF1KA1714.seq Check: 5906 from: 1 to: 6494
pF1KA1714 6494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1429 2390 1429
Size: 961 5533
AccI GT'mk_AC
Cuts at: 3532 3532
Size: 6494
Acc65I G'GTAC_C
Cuts at: 3510 3510
Size: 6494
AclI AA'CG_TT
Cuts at: 3488 6334 3488
Size: 2846 3648
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 222 4319 4751 5498 5766 98
Size: 124 4097 432 747 268 826
Fragments arranged by size:
4097 826 747 432 268 124
AfeI AGC'GCT
Cuts at: 2131 2131
Size: 6494
AflII C'TTAA_G
Cuts at: 1375 1375
Size: 6494
AflIII A'CryG_T
Cuts at: 2555 3846 4950 5927 2555
Size: 1291 1104 977 3122
Fragments arranged by size:
3122 1291 1104 977
AgeI A'CCGG_T
Cuts at: 4882 4882
Size: 6494
AhdI GACnn_n'nnGTC
Cuts at: 1281 1281
Size: 6494
AleI CACnn'nnGTG
Cuts at: 2653 2653
Size: 6494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3006 3006
Size: 6494
AlwNI CAG_nnn'CTG
Cuts at: 1444 1539 1772 2145 3799 5366 1444
Size: 95 233 373 1654 1567 2572
Fragments arranged by size:
2572 1654 1567 373 233 95
ApaLI G'TGCA_C
Cuts at: 532 5264 532
Size: 4732 1762
ApoI r'AATT_y
Cuts at: 739 764 1049 2484 3498 5847 6103 739
Size: 25 285 1435 1014 2349 256 1130
Fragments arranged by size:
2349 1435 1130 1014 285 256 25
AseI AT'TA_AT
Cuts at: 20 814 20
Size: 794 5700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6494
AvaI C'yCGr_G
Cuts at: 273 3514 273
Size: 3241 3253
AvrII C'CTAG_G
Cuts at: 226 598 226
Size: 372 6122
BamHI G'GATC_C
Cuts at: 1680 3519 1680
Size: 1839 4655
BanI G'GyrC_C
Cuts at: 2041 2054 2171 2735 3510 3924 4172 4207 2041
Size: 13 117 564 775 414 248 35 4328
Fragments arranged by size:
4328 775 564 414 248 117 35 13
BanII G_rGCy'C
Cuts at: 172 415 944 1237 3286 3508 4538 172
Size: 243 529 293 2049 222 1030 2128
Fragments arranged by size:
2128 2049 1030 529 293 243 222
BbeI G_GCGC'C
Cuts at: 2175 3928 4176 2175
Size: 1753 248 4493
Fragments arranged by size:
4493 1753 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3252 3286 3252
Size: 34 6460
BciVI GTATCCnnnnn_n'
Cuts at: 4392 5153 6494 4392
Size: 761 1341 4392
Fragments arranged by size:
4392 1341 761
BclI T'GATC_A
Cuts at: 2784 3230 4014 2784
Size: 446 784 5264
Fragments arranged by size:
5264 784 446
BfrBI ATG'CAT
Cuts at: 2900 3376 2900
Size: 476 6018
BglII A'GATC_T
Cuts at: 4009 6046 4009
Size: 2037 4457
BlpI GC'TnA_GC
Cuts at: 857 3606 857
Size: 2749 3745
Bme1580I G_kGCm'C
Cuts at: 536 2046 2057 2828 4119 4212 5268 536
Size: 1510 11 771 1291 93 1056 1762
Fragments arranged by size:
1762 1510 1291 1056 771 93 11
BmgBI CAC'GTC
Cuts at: 1348 2556 1348
Size: 1208 5286
BmrI ACTGGGnnnn_n'
Cuts at: 429 1791 2250 2455 3706 3787 4122 6266 429
Size: 1362 459 205 1251 81 335 2144 657
Fragments arranged by size:
2144 1362 1251 657 459 335 205 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4861 4861
Size: 6494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1221 1711 3162 529
Size: 692 490 1451 3861
Fragments arranged by size:
3861 1451 692 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 3670 4549 5041 5339 118
Size: 3552 879 492 298 1273
Fragments arranged by size:
3552 1273 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1843 2912 6198 1843
Size: 1069 3286 2139
Fragments arranged by size:
3286 2139 1069
BsaAI yAC'GTr
Cuts at: 2742 3847 2742
Size: 1105 5389
BsaBI GATnn'nnATC
Cuts at: 6 619 3359 4008 6045 6
Size: 613 2740 649 2037 455
Fragments arranged by size:
2740 2037 649 613 455
BsaHI Gr'CG_yC
Cuts at: 1988 2172 2959 3925 4173 4875 6371 1988
Size: 184 787 966 248 702 1496 2111
Fragments arranged by size:
2111 1496 966 787 702 248 184
BsaWI w'CCGG_w
Cuts at: 3683 4204 4882 5156 5303 5714 3683
Size: 521 678 274 147 411 4463
Fragments arranged by size:
4463 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 861 891 1118 1148 1798 1828 2962 2992 3243
Size: 30 227 30 650 30 1134 30 251
Cuts at: 3243 3273 861
Size: 30 4082
Fragments arranged by size:
4082 1134 650 251 227 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 237 934 3120 237
Size: 697 2186 3611
Fragments arranged by size:
3611 2186 697
BseYI C'CCAG_C
Cuts at: 1707 1765 2047 3919 4677 5254 5750 1707
Size: 58 282 1872 758 577 496 2451
Fragments arranged by size:
2451 1872 758 577 496 282 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2026 2722 3002 2026
Size: 696 280 5518
Fragments arranged by size:
5518 696 280
BsiEI CG_ry'CG
Cuts at: 104 4036 4082 5290 104
Size: 3932 46 1208 1308
Fragments arranged by size:
3932 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 415 536 681 944 1237 1553 2381 3286 3508
Size: 121 145 263 293 316 828 905 222
Cuts at: 3508 4286 4476 5268 415
Size: 778 190 792 1641
Fragments arranged by size:
1641 905 828 792 778 316 293 263
222 190 145 121
BsiWI C'GTAC_G
Cuts at: 2081 2081
Size: 6494
BsmI GAATG_Cn'
Cuts at: 924 1075 2900 924
Size: 151 1825 4518
Fragments arranged by size:
4518 1825 151
BsmBI CGTCTCn'nnnn_
Cuts at: 716 2777 716
Size: 2061 4433
Bsp1286I G_dGCh'C
Cuts at: 172 415 536 681 944 1237 1553 2046 2057
Size: 243 121 145 263 293 316 493 11
Cuts at: 2057 2381 2828 3286 3508 4119 4212 4286 4476
Size: 324 447 458 222 611 93 74 190
Cuts at: 4476 4538 5268 172
Size: 62 730 1398
Fragments arranged by size:
1398 730 611 493 458 447 324 316
293 263 243 222 190 145 121 93
74 62 11
BspEI T'CCGG_A
Cuts at: 5714 5714
Size: 6494
BspHI T'CATG_A
Cuts at: 1172 5670 1172
Size: 4498 1996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 945 1429 1861 2390 3544 3758 4060 4441 945
Size: 484 432 529 1154 214 302 381 2998
Fragments arranged by size:
2998 1154 529 484 432 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4786 4840 6324 6489 50
Size: 4736 54 1484 165 55
Fragments arranged by size:
4736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 765 858 3024 4406 765
Size: 93 2166 1382 2853
Fragments arranged by size:
2853 2166 1382 93
BsrFI r'CCGG_y
Cuts at: 4492 4673 4882 4492
Size: 181 209 6104
Fragments arranged by size:
6104 209 181
BssSI C'ACGA_G
Cuts at: 2183 4765 5123 2183
Size: 2582 358 3554
Fragments arranged by size:
3554 2582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3118 3871 3118
Size: 753 5741
BstBI TT'CG_AA
Cuts at: 1302 4855 1302
Size: 3553 2941
BstEII G'GTnAC_C
Cuts at: 423 2028 3051 423
Size: 1605 1023 3866
Fragments arranged by size:
3866 1605 1023
BstXI CCAn_nnnn'nTGG
Cuts at: 1017 2204 1017
Size: 1187 5307
BstYI r'GATC_y
Cuts at: 1 1020 1166 1680 2934 3519 3986 4009 4344
Size: 1019 146 514 1254 585 467 23 335
Cuts at: 4344 4590 5602 5688 5700 6046 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1254 1019 1012 585 514 467 449 346
335 246 146 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1648 1648
Size: 6494
BtgI C'CryG_G
Cuts at: 107 267 968 2111 2463 2535 107
Size: 160 701 1143 352 72 4066
Fragments arranged by size:
4066 1143 701 352 160 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4444 4625 4788 4444
Size: 181 163 6150
Fragments arranged by size:
6150 181 163
BtsI GCAGTG_nn'
Cuts at: 380 1468 2452 2546 2988 3737 380
Size: 1088 984 94 442 749 3137
Fragments arranged by size:
3137 1088 984 749 442 94
DraI TTT'AAA
Cuts at: 710 744 3393 3493 710
Size: 34 2649 100 3711
Fragments arranged by size:
3711 2649 100 34
DraIII CAC_nnn'GTG
Cuts at: 1473 2742 3423 1473
Size: 1269 681 4544
Fragments arranged by size:
4544 1269 681
DrdI GACnn_nn'nnGTC
Cuts at: 955 2359 3011 4200 5058 955
Size: 1404 652 1189 858 2391
Fragments arranged by size:
2391 1404 1189 858 652
EaeI y'GGCC_r
Cuts at: 2331 2870 4079 4253 4644 4671 6016 2331
Size: 539 1209 174 391 27 1345 2809
Fragments arranged by size:
2809 1345 1209 539 391 174 27
EagI C'GGCC_G
Cuts at: 4079 4079
Size: 6494
EarI CTCTTCn'nnn_
Cuts at: 291 1408 2780 2860 4517 4727 291
Size: 1117 1372 80 1657 210 2058
Fragments arranged by size:
2058 1657 1372 1117 210 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 4702 5012 5158 6304 2140
Size: 2562 310 146 1146 2330
Fragments arranged by size:
2562 2330 1146 310 146
EcoICRI GAG'CTC
Cuts at: 413 942 1235 3284 3506 413
Size: 529 293 2049 222 3401
Fragments arranged by size:
3401 2049 529 293 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 222 529 1221 1711 3162 4319 4751 5498
Size: 124 307 692 490 1451 1157 432 747
Cuts at: 5498 5766 98
Size: 268 826
Fragments arranged by size:
1451 1157 826 747 692 490 432 307
268 124
EcoNI CCTnn'n_nnAGG
Cuts at: 225 225
Size: 6494
EcoO109I rG'GnC_Cy
Cuts at: 1722 3633 1722
Size: 1911 4583
EcoRI G'AATT_C
Cuts at: 764 1049 2484 3498 764
Size: 285 1435 1014 3760
Fragments arranged by size:
3760 1435 1014 285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 844 849 4513 844
Size: 5 3664 2825
Fragments arranged by size:
3664 2825 5
FspI TGC'GCA
Cuts at: 4275 4275
Size: 6494
HaeII r_GCGC'y
Cuts at: 1708 2133 2175 3928 4176 5198 6177 1708
Size: 425 42 1753 248 1022 979 2025
Fragments arranged by size:
2025 1753 1022 979 425 248 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1267 1299 1585 1617 1798 1830 3215 3247 4336
Size: 32 286 32 181 32 1385 32 1089
Cuts at: 4336 4366 4368 4398 1267
Size: 30 2 30 3363
Fragments arranged by size:
3363 1385 1089 286 181 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 960 3533 960
Size: 2573 3921
HindIII A'AGCT_T
Cuts at: 826 1089 826
Size: 263 6231
KasI G'GCGC_C
Cuts at: 2171 3924 4172 2171
Size: 1753 248 4493
Fragments arranged by size:
4493 1753 248
KpnI G_GTAC'C
Cuts at: 3514 3514
Size: 6494
MluI A'CGCG_T
Cuts at: 5927 5927
Size: 6494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1078 2193 5165 5349 1078
Size: 1115 2972 184 2223
Fragments arranged by size:
2972 2223 1115 184
MscI TGG'CCA
Cuts at: 4255 4255
Size: 6494
MslI CAynn'nnrTG
Cuts at: 1502 2653 3206 4483 4610 1502
Size: 1151 553 1277 127 3386
Fragments arranged by size:
3386 1277 1151 553 127
MspA1I CmG'CkG
Cuts at: 1528 2051 2089 2103 2113 2142 2465 3605 3919
Size: 523 38 14 10 29 323 1140 314
Cuts at: 3919 4279 5292 5537 1528
Size: 360 1013 245 2485
Fragments arranged by size:
2485 1140 1013 523 360 323 314 245
38 29 14 10
NaeI GCC'GGC
Cuts at: 4675 4675
Size: 6494
NarI GG'CG_CC
Cuts at: 2172 3925 4173 2172
Size: 1753 248 4493
Fragments arranged by size:
4493 1753 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6494
NdeI CA'TA_TG
Cuts at: 1499 1499
Size: 6494
NgoMIV G'CCGG_C
Cuts at: 4673 4673
Size: 6494
NsiI A_TGCA'T
Cuts at: 2902 3378 2902
Size: 476 6018
NspI r_CATG'y
Cuts at: 2644 3547 4047 4954 2644
Size: 903 500 907 4184
Fragments arranged by size:
4184 907 903 500
PacI TTA_AT'TAA
Cuts at: 818 818
Size: 6494
PasI CC'CwG_GG
Cuts at: 894 1626 1950 894
Size: 732 324 5438
Fragments arranged by size:
5438 732 324
PciI A'CATG_T
Cuts at: 4950 4950
Size: 6494
PfoI T'CCnGG_A
Cuts at: 504 1237 504
Size: 733 5761
PmeI GTTT'AAAC
Cuts at: 3493 3493
Size: 6494
PmlI CAC'GTG
Cuts at: 2742 2742
Size: 6494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5633 5665 5633
Size: 32 6462
PpuMI rG'GwC_Cy
Cuts at: 1722 1722
Size: 6494
PsiI TTA'TAA
Cuts at: 1121 1121
Size: 6494
PstI C_TGCA'G
Cuts at: 2915 2995 3541 4226 2915
Size: 80 546 685 5183
Fragments arranged by size:
5183 685 546 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6494
PvuII CAG'CTG
Cuts at: 1528 2103 3919 4279 1528
Size: 575 1816 360 3743
Fragments arranged by size:
3743 1816 575 360
RsrII CG'GwC_CG
Cuts at: 2108 4689 2108
Size: 2581 3913
SacI G_AGCT'C
Cuts at: 415 944 1237 3286 3508 415
Size: 529 293 2049 222 3401
Fragments arranged by size:
3401 2049 529 293 222
SacII CC_GC'GG
Cuts at: 2114 2466 2114
Size: 352 6142
SalI G'TCGA_C
Cuts at: 3531 3531
Size: 6494
SapI GCTCTTCn'nnn_
Cuts at: 2860 4517 4727 2860
Size: 1657 210 4627
Fragments arranged by size:
4627 1657 210
SbfI CC_TGCA'GG
Cuts at: 3541 3541
Size: 6494
ScaI AGT'ACT
Cuts at: 6011 6011
Size: 6494
SfcI C'TryA_G
Cuts at: 33 537 2911 2991 3537 4222 5215 5406 33
Size: 504 2374 80 546 685 993 191 1121
Fragments arranged by size:
2374 1121 993 685 546 504 191 80
SfoI GGC'GCC
Cuts at: 2173 3926 4174 2173
Size: 1753 248 4493
Fragments arranged by size:
4493 1753 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6494
SmaI CCC'GGG
Cuts at: 275 3516 275
Size: 3241 3253
SmlI C'TyrA_G
Cuts at: 133 1375 3649 4564 5056 5318 133
Size: 1242 2274 915 492 262 1309
Fragments arranged by size:
2274 1309 1242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 2347 67
Size: 2280 4214
SphI G_CATG'C
Cuts at: 2644 3547 4047 2644
Size: 903 500 5091
Fragments arranged by size:
5091 903 500
StyI C'CwwG_G
Cuts at: 107 226 360 396 598 1423 3628 3982 107
Size: 119 134 36 202 825 2205 354 2619
Fragments arranged by size:
2619 2205 825 354 202 134 119 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 411 1088 2724 2894 4077 4210 4669 4878
Size: 2 677 1636 170 1183 133 459 209
Cuts at: 4878 409
Size: 2025
Fragments arranged by size:
2025 1636 1183 677 459 209 170 133
2
TatI w'GTAC_w
Cuts at: 1692 1967 2272 6009 1692
Size: 275 305 3737 2177
Fragments arranged by size:
3737 2177 305 275
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 861 893 910 942 1199 1231 1459 1491 2962
Size: 32 17 32 257 32 228 32 1471
Cuts at: 2962 2994 3227 3243 3259 3275 6011 6043 861
Size: 32 233 16 16 16 2736 32 1312
Fragments arranged by size:
2736 1471 1312 257 233 228 32 32
32 32 32 32 17 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 4291 4291
Size: 6494
XbaI T'CTAG_A
Cuts at: 3525 3525
Size: 6494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 403 1245 2204 2857 3989 114
Size: 289 842 959 653 1132 2619
Fragments arranged by size:
2619 1132 959 842 653 289
XmaI C'CCGG_G
Cuts at: 273 3514 273
Size: 3241 3253
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AloI ApaI AscI BaeI BbsI BbvCI BglI
BmtI Bpu10I BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI PflMI
PshAI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI