Restriction map for
pF1KA1726
(Circular) (Six-base) MAPSORT of: pF1KA1726.seq Check: 5727 from: 1 to: 5765
pF1KA1726 5765 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2246 2246
Size: 5765
AccI GT'mk_AC
Cuts at: 1607 1865 2803 1607
Size: 258 938 4569
Fragments arranged by size:
4569 938 258
Acc65I G'GTAC_C
Cuts at: 2474 2781 2474
Size: 307 5458
AclI AA'CG_TT
Cuts at: 5605 5605
Size: 5765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 401 557 656 3590 4022 4375 5037 374
Size: 27 156 99 2934 432 353 662 1102
Fragments arranged by size:
2934 1102 662 432 353 156 99 27
AflII C'TTAA_G
Cuts at: 619 619
Size: 5765
AflIII A'CryG_T
Cuts at: 190 3117 4917 5198 190
Size: 2927 1800 281 757
Fragments arranged by size:
2927 1800 757 281
AgeI A'CCGG_T
Cuts at: 2045 2045
Size: 5765
AleI CACnn'nnGTG
Cuts at: 2514 2514
Size: 5765
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 2183
Size: 32 5733
AlwNI CAG_nnn'CTG
Cuts at: 1441 3070 4508 1441
Size: 1629 1438 2698
Fragments arranged by size:
2698 1629 1438
ApaLI G'TGCA_C
Cuts at: 4603 4603
Size: 5765
ApoI r'AATT_y
Cuts at: 393 515 1031 2085 2145 2769 5118 5374 393
Size: 122 516 1054 60 624 2349 256 784
Fragments arranged by size:
2349 1054 784 624 516 256 122 60
AseI AT'TA_AT
Cuts at: 20 1234 20
Size: 1214 4551
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5765
AvaI C'yCGr_G
Cuts at: 2604 2785 2604
Size: 181 5584
AvrII C'CTAG_G
Cuts at: 222 2325 222
Size: 2103 3662
BamHI G'GATC_C
Cuts at: 2573 2790 2573
Size: 217 5548
BanI G'GyrC_C
Cuts at: 2257 2316 2474 2781 3195 3443 3478 2257
Size: 59 158 307 414 248 35 4544
Fragments arranged by size:
4544 414 307 248 158 59 35
BanII G_rGCy'C
Cuts at: 2779 3809 2779
Size: 1030 4735
BbeI G_GCGC'C
Cuts at: 2261 3199 3447 2261
Size: 938 248 4579
Fragments arranged by size:
4579 938 248
BciVI GTATCCnnnnn_n'
Cuts at: 309 1853 3663 4719 5765 309
Size: 1544 1810 1056 1046 309
Fragments arranged by size:
1810 1544 1056 1046 309
BclI T'GATC_A
Cuts at: 399 735 3285 399
Size: 336 2550 2879
Fragments arranged by size:
2879 2550 336
BfrBI ATG'CAT
Cuts at: 1894 1894
Size: 5765
BglI GCCn_nnn'nGGC
Cuts at: 1080 2426 2568 1080
Size: 1346 142 4277
Fragments arranged by size:
4277 1346 142
BglII A'GATC_T
Cuts at: 3280 5317 3280
Size: 2037 3728
BlpI GC'TnA_GC
Cuts at: 2877 2877
Size: 5765
Bme1580I G_kGCm'C
Cuts at: 3390 3483 4607 3390
Size: 93 1124 4548
Fragments arranged by size:
4548 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1292 2977 3058 3393 5537 1292
Size: 1685 81 335 2144 1520
Fragments arranged by size:
2144 1685 1520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4132 4132
Size: 5765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1763 628
Size: 1135 4630
Bpu10I CC'TnA_GC
Cuts at: 2549 2549
Size: 5765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 2941 3820 4534 4832 487
Size: 2454 879 714 298 1420
Fragments arranged by size:
2454 1420 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 482 840 5469 482
Size: 358 4629 778
Fragments arranged by size:
4629 778 358
BsaAI yAC'GTr
Cuts at: 193 1039 2031 3118 193
Size: 846 992 1087 2840
Fragments arranged by size:
2840 1087 992 846
BsaBI GATnn'nnATC
Cuts at: 6 2635 3279 5316 6
Size: 2629 644 2037 455
Fragments arranged by size:
2629 2037 644 455
BsaHI Gr'CG_yC
Cuts at: 349 2258 2465 2708 3196 3444 4146 5642 349
Size: 1909 207 243 488 248 702 1496 472
Fragments arranged by size:
1909 1496 702 488 472 248 243 207
BsaWI w'CCGG_w
Cuts at: 2045 2954 3475 4153 4564 4711 4985 2045
Size: 909 521 678 411 147 274 2825
Fragments arranged by size:
2825 909 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 692 722 1487 1517 692
Size: 30 765 30 4940
Fragments arranged by size:
4940 765 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1110 2095 2204 2282 1110
Size: 985 109 78 4593
Fragments arranged by size:
4593 985 109 78
BseYI C'CCAG_C
Cuts at: 1683 2438 2711 2747 2751 3190 3948 4613 5021
Size: 755 273 36 4 439 758 665 408
Cuts at: 5021 1683
Size: 2427
Fragments arranged by size:
2427 758 755 665 439 408 273 36
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 2218
Size: 5765
BsiEI CG_ry'CG
Cuts at: 104 3307 3353 4583 104
Size: 3203 46 1230 1286
Fragments arranged by size:
3203 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 259 1569 2779 3557 3747 4607 259
Size: 1310 1210 778 190 860 1417
Fragments arranged by size:
1417 1310 1210 860 778 190
BsmI GAATG_Cn'
Cuts at: 131 149 366 1896 2594 131
Size: 18 217 1530 698 3302
Fragments arranged by size:
3302 1530 698 217 18
Bsp1286I G_dGCh'C
Cuts at: 259 1569 2779 3390 3483 3557 3747 3809 4607
Size: 1310 1210 611 93 74 190 62 798
Cuts at: 4607 259
Size: 1417
Fragments arranged by size:
1417 1310 1210 798 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 402 2681 4197 402
Size: 2279 1516 1970
Fragments arranged by size:
2279 1970 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 2246 2815 3029 3331 3712 464
Size: 1782 569 214 302 381 2517
Fragments arranged by size:
2517 1782 569 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4057 4111 5595 5760 50
Size: 4007 54 1484 165 55
Fragments arranged by size:
4007 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 516 876 884 1734 3677 516
Size: 360 8 850 1943 2604
Fragments arranged by size:
2604 1943 850 360 8
BsrFI r'CCGG_y
Cuts at: 2045 2477 3763 3944 2045
Size: 432 1286 181 3866
Fragments arranged by size:
3866 1286 432 181
BssSI C'ACGA_G
Cuts at: 2346 4036 4744 2346
Size: 1690 708 3367
Fragments arranged by size:
3367 1690 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3142 3142
Size: 5765
BstBI TT'CG_AA
Cuts at: 4126 4126
Size: 5765
BstXI CCAn_nnnn'nTGG
Cuts at: 2736 2736
Size: 5765
BstYI r'GATC_y
Cuts at: 1 1584 2573 2598 2790 3257 3280 3615 3861
Size: 1583 989 25 192 467 23 335 246
Cuts at: 3861 4167 4179 4265 5317 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1583 1052 989 467 449 335 306 246
192 86 25 23 12
Bsu36I CC'TnA_GG
Cuts at: 1760 1760
Size: 5765
BtgI C'CryG_G
Cuts at: 107 885 107
Size: 778 4987
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3715 3896 4059 3715
Size: 181 163 5421
Fragments arranged by size:
5421 181 163
BtsI GCAGTG_nn'
Cuts at: 935 1998 2025 3008 935
Size: 1063 27 983 3692
Fragments arranged by size:
3692 1063 983 27
DraI TTT'AAA
Cuts at: 531 2764 531
Size: 2233 3532
DrdI GACnn_nn'nnGTC
Cuts at: 1861 3471 4815 1861
Size: 1610 1344 2811
Fragments arranged by size:
2811 1610 1344
EaeI y'GGCC_r
Cuts at: 237 2342 3350 3524 3915 3942 5287 237
Size: 2105 1008 174 391 27 1345 715
Fragments arranged by size:
2105 1345 1008 715 391 174 27
EagI C'GGCC_G
Cuts at: 3350 3350
Size: 5765
EarI CTCTTCn'nnn_
Cuts at: 115 350 533 2222 3788 3998 115
Size: 235 183 1689 1566 210 1882
Fragments arranged by size:
1882 1689 1566 235 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2586 3973 4715 4861 5575 2586
Size: 1387 742 146 714 2776
Fragments arranged by size:
2776 1387 742 714 146
EcoICRI GAG'CTC
Cuts at: 2777 2777
Size: 5765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 401 557 628 656 1763 3590 4022 4375
Size: 27 156 71 28 1107 1827 432 353
Cuts at: 4375 5037 374
Size: 662 1102
Fragments arranged by size:
1827 1107 1102 662 432 353 156 71
28 27
EcoO109I rG'GnC_Cy
Cuts at: 2333 2904 2333
Size: 571 5194
EcoRI G'AATT_C
Cuts at: 2769 2769
Size: 5765
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1645 2426 3784 1645
Size: 781 1358 3626
Fragments arranged by size:
3626 1358 781
FspI TGC'GCA
Cuts at: 3546 3546
Size: 5765
HaeII r_GCGC'y
Cuts at: 2261 3199 3447 4677 5448 2261
Size: 938 248 1230 771 2578
Fragments arranged by size:
2578 1230 938 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 692 724 2272 2304 2347 2379 3607
Size: 32 269 32 1548 32 43 32 1228
Cuts at: 3607 3637 3639 3669 391
Size: 30 2 30 2487
Fragments arranged by size:
2487 1548 1228 269 43 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 293 1866 2098 2804 293
Size: 1573 232 706 3254
Fragments arranged by size:
3254 1573 706 232
HindIII A'AGCT_T
Cuts at: 1627 1627
Size: 5765
KasI G'GCGC_C
Cuts at: 2257 3195 3443 2257
Size: 938 248 4579
Fragments arranged by size:
4579 938 248
KpnI G_GTAC'C
Cuts at: 2478 2785 2478
Size: 307 5458
MfeI C'AATT_G
Cuts at: 415 415
Size: 5765
MluI A'CGCG_T
Cuts at: 5198 5198
Size: 5765
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 4524 4708 1127
Size: 3397 184 2184
Fragments arranged by size:
3397 2184 184
MscI TGG'CCA
Cuts at: 2344 3526 2344
Size: 1182 4583
MslI CAynn'nnrTG
Cuts at: 245 2514 2734 3754 3881 245
Size: 2269 220 1020 127 2129
Fragments arranged by size:
2269 2129 1020 220 127
MspA1I CmG'CkG
Cuts at: 280 1431 2251 2270 2751 2876 3190 3550 4334
Size: 1151 820 19 481 125 314 360 784
Cuts at: 4334 4579 280
Size: 245 1466
Fragments arranged by size:
1466 1151 820 784 481 360 314 245
125 19
NaeI GCC'GGC
Cuts at: 3946 3946
Size: 5765
NarI GG'CG_CC
Cuts at: 2258 3196 3444 2258
Size: 938 248 4579
Fragments arranged by size:
4579 938 248
NcoI C'CATG_G
Cuts at: 107 885 107
Size: 778 4987
NdeI CA'TA_TG
Cuts at: 1362 1362
Size: 5765
NgoMIV G'CCGG_C
Cuts at: 3944 3944
Size: 5765
NsiI A_TGCA'T
Cuts at: 1896 1896
Size: 5765
NspI r_CATG'y
Cuts at: 2106 2818 3318 4921 2106
Size: 712 500 1603 2950
Fragments arranged by size:
2950 1603 712 500
PciI A'CATG_T
Cuts at: 4917 4917
Size: 5765
PflMI CCAn_nnn'nTGG
Cuts at: 2455 2584 2455
Size: 129 5636
PmeI GTTT'AAAC
Cuts at: 2764 2764
Size: 5765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 5733
PpuMI rG'GwC_Cy
Cuts at: 2333 2333
Size: 5765
PshAI GACnn'nnGTC
Cuts at: 1603 1603
Size: 5765
PstI C_TGCA'G
Cuts at: 475 913 1990 2144 2812 3497 475
Size: 438 1077 154 668 685 2743
Fragments arranged by size:
2743 1077 685 668 438 154
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5765
PvuII CAG'CTG
Cuts at: 280 2270 2751 3190 3550 280
Size: 1990 481 439 360 2495
Fragments arranged by size:
2495 1990 481 439 360
RsrII CG'GwC_CG
Cuts at: 3960 3960
Size: 5765
SacI G_AGCT'C
Cuts at: 2779 2779
Size: 5765
SalI G'TCGA_C
Cuts at: 1864 2802 1864
Size: 938 4827
SapI GCTCTTCn'nnn_
Cuts at: 3788 3998 3788
Size: 210 5555
SbfI CC_TGCA'GG
Cuts at: 2812 2812
Size: 5765
ScaI AGT'ACT
Cuts at: 5282 5282
Size: 5765
SexAI A'CCwGG_T
Cuts at: 1654 1654
Size: 5765
SfcI C'TryA_G
Cuts at: 33 471 909 1176 1986 2140 2808 3493 4461
Size: 438 438 267 810 154 668 685 968
Cuts at: 4461 4652 33
Size: 191 1146
Fragments arranged by size:
1146 968 810 685 668 438 438 267
191 154
SfoI GGC'GCC
Cuts at: 2259 3197 3445 2259
Size: 938 248 4579
Fragments arranged by size:
4579 938 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5765
SmaI CCC'GGG
Cuts at: 2787 2787
Size: 5765
SmlI C'TyrA_G
Cuts at: 502 619 2920 3835 4549 4811 502
Size: 117 2301 915 714 262 1456
Fragments arranged by size:
2301 1456 915 714 262 117
SnaBI TAC'GTA
Cuts at: 1039 1039
Size: 5765
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5765
SphI G_CATG'C
Cuts at: 2818 3318 2818
Size: 500 5265
SspI AAT'ATT
Cuts at: 1399 1399
Size: 5765
StuI AGG'CCT
Cuts at: 2429 2429
Size: 5765
StyI C'CwwG_G
Cuts at: 107 222 286 557 885 1532 2325 2336 2899
Size: 115 64 271 328 647 793 11 563
Cuts at: 2899 3253 107
Size: 354 2619
Fragments arranged by size:
2619 793 647 563 354 328 271 115
64 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3348 3481 3940 4149 3348
Size: 133 459 209 4964
Fragments arranged by size:
4964 459 209 133
TatI w'GTAC_w
Cuts at: 187 654 748 5280 187
Size: 467 94 4532 672
Fragments arranged by size:
4532 672 467 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1487 1519 5282 5314 1487
Size: 32 3763 32 1938
Fragments arranged by size:
3763 1938 32 32
Tth111I GACn'n_nGTC
Cuts at: 3562 3562
Size: 5765
XbaI T'CTAG_A
Cuts at: 1462 2796 1462
Size: 1334 4431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 284 1644 3260 284
Size: 1360 1616 2789
Fragments arranged by size:
2789 1616 1360
XmaI C'CCGG_G
Cuts at: 2785 2785
Size: 5765
XmnI GAAnn'nnTTC
Cuts at: 644 1213 1628 1968 644
Size: 569 415 340 4441
Fragments arranged by size:
4441 569 415 340
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI ApaI AscI BaeI BbsI
BbvCI BcgI BmgBI BmtI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NruI PacI PasI
PfoI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SwaI XhoI ZraI