Restriction map for
pF1KA1738
(Circular) (Six-base) MAPSORT of: pF1KA1738.seq Check: 1646 from: 1 to: 6494
pF1KA1738 6494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2441 2441
Size: 6494
AccI GT'mk_AC
Cuts at: 694 3532 694
Size: 2838 3656
Acc65I G'GTAC_C
Cuts at: 3510 3510
Size: 6494
AclI AA'CG_TT
Cuts at: 6334 6334
Size: 6494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1297 1312 1768 2057 2905 3053 4319 4751
Size: 230 15 456 289 848 148 1266 432
Cuts at: 4751 5498 5766 1067
Size: 747 268 1795
Fragments arranged by size:
1795 1266 848 747 456 432 289 268
230 148 15
AfeI AGC'GCT
Cuts at: 2964 2964
Size: 6494
AflII C'TTAA_G
Cuts at: 1842 1842
Size: 6494
AflIII A'CryG_T
Cuts at: 3270 3846 4950 5927 3270
Size: 576 1104 977 3837
Fragments arranged by size:
3837 1104 977 576
AgeI A'CCGG_T
Cuts at: 4882 4882
Size: 6494
AhdI GACnn_n'nnGTC
Cuts at: 2043 2043
Size: 6494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 244
Size: 32 6462
AlwNI CAG_nnn'CTG
Cuts at: 3031 3799 5366 3031
Size: 768 1567 4159
Fragments arranged by size:
4159 1567 768
ApaI G_GGCC'C
Cuts at: 536 536
Size: 6494
ApaLI G'TGCA_C
Cuts at: 175 5264 175
Size: 5089 1405
ApoI r'AATT_y
Cuts at: 2844 3498 5847 6103 2844
Size: 654 2349 256 3235
Fragments arranged by size:
3235 2349 654 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6494
AvaI C'yCGr_G
Cuts at: 197 2830 2921 3514 197
Size: 2633 91 593 3177
Fragments arranged by size:
3177 2633 593 91
BamHI G'GATC_C
Cuts at: 3519 3519
Size: 6494
BanI G'GyrC_C
Cuts at: 2324 3510 3924 4172 4207 2324
Size: 1186 414 248 35 4611
Fragments arranged by size:
4611 1186 414 248 35
BanII G_rGCy'C
Cuts at: 536 1235 2491 2702 2999 3508 4538 536
Size: 699 1256 211 297 509 1030 2492
Fragments arranged by size:
2492 1256 1030 699 509 297 211
BbeI G_GCGC'C
Cuts at: 3928 4176 3928
Size: 248 6246
BbsI GAAGACnn'nnnn_
Cuts at: 332 515 757 1300 1534 2987 332
Size: 183 242 543 234 1453 3839
Fragments arranged by size:
3839 1453 543 242 234 183
BbvCI CC'TCA_GC
Cuts at: 2093 2093
Size: 6494
BciVI GTATCCnnnnn_n'
Cuts at: 792 4392 5153 6494 792
Size: 3600 761 1341 792
Fragments arranged by size:
3600 1341 792 761
BclI T'GATC_A
Cuts at: 2769 3480 4014 2769
Size: 711 534 5249
Fragments arranged by size:
5249 711 534
BglII A'GATC_T
Cuts at: 1717 2143 4009 6046 1717
Size: 426 1866 2037 2165
Fragments arranged by size:
2165 2037 1866 426
BlpI GC'TnA_GC
Cuts at: 3606 3606
Size: 6494
Bme1580I G_kGCm'C
Cuts at: 179 536 950 1118 2327 4119 4212 5268 179
Size: 357 414 168 1209 1792 93 1056 1405
Fragments arranged by size:
1792 1405 1209 1056 414 357 168 93
BmrI ACTGGGnnnn_n'
Cuts at: 545 3706 3787 4122 6266 545
Size: 3161 81 335 2144 773
Fragments arranged by size:
3161 2144 773 335 81
BmtI G_CTAG'C
Cuts at: 545 545
Size: 6494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1512 2973 4861 1512
Size: 1461 1888 3145
Fragments arranged by size:
3145 1888 1461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2291 2731 2943 3373 1333
Size: 958 440 212 430 4454
Fragments arranged by size:
4454 958 440 430 212
Bpu10I CC'TnA_GC
Cuts at: 537 2093 537
Size: 1556 4938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 1849 3670 4549 5041 5339 1423
Size: 426 1821 879 492 298 2578
Fragments arranged by size:
2578 1821 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 2827 6198 2827
Size: 3371 3123
BsaAI yAC'GTr
Cuts at: 180 3847 180
Size: 3667 2827
BsaBI GATnn'nnATC
Cuts at: 6 4008 6045 6
Size: 4002 2037 455
Fragments arranged by size:
4002 2037 455
BsaHI Gr'CG_yC
Cuts at: 984 3925 4173 4875 6371 984
Size: 2941 248 702 1496 1107
Fragments arranged by size:
2941 1496 1107 702 248
BsaWI w'CCGG_w
Cuts at: 3683 4204 4882 5156 5303 5714 3683
Size: 521 678 274 147 411 4463
Fragments arranged by size:
4463 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 1141 1171 2359 2389 3005 3035 3346
Size: 30 867 30 1188 30 616 30 311
Cuts at: 3346 3376 244
Size: 30 3362
Fragments arranged by size:
3362 1188 867 616 311 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 253 256 265 268 271 292 1165 1168
Size: 3 3 9 3 3 21 873 3
Cuts at: 1168 2380 2499 2569 2961 3010 250
Size: 1212 119 70 392 49 3734
Fragments arranged by size:
3734 1212 873 392 119 70 49 21
9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2521 2998 3919 4677 5254 5750 2521
Size: 477 921 758 577 496 3265
Fragments arranged by size:
3265 921 758 577 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1525 2279 202
Size: 1323 754 4417
Fragments arranged by size:
4417 1323 754
BsiEI CG_ry'CG
Cuts at: 104 698 1502 4036 4082 5290 104
Size: 594 804 2534 46 1208 1308
Fragments arranged by size:
2534 1308 1208 804 594 46
BsiHKAI G_wGCw'C
Cuts at: 179 1454 1671 2702 3508 4286 4476 5268 179
Size: 1275 217 1031 806 778 190 792 1405
Fragments arranged by size:
1405 1275 1031 806 792 778 217 190
BsmI GAATG_Cn'
Cuts at: 2586 2926 2586
Size: 340 6154
BsmBI CGTCTCn'nnnn_
Cuts at: 438 438
Size: 6494
Bsp1286I G_dGCh'C
Cuts at: 179 536 950 1118 1235 1454 1671 2327 2491
Size: 357 414 168 117 219 217 656 164
Cuts at: 2491 2702 2999 3508 4119 4212 4286 4476 4538
Size: 211 297 509 611 93 74 190 62
Cuts at: 4538 5268 179
Size: 730 1405
Fragments arranged by size:
1405 730 656 611 509 414 357 297
219 217 211 190 168 164 117 93
74 62
BspEI T'CCGG_A
Cuts at: 5714 5714
Size: 6494
BspHI T'CATG_A
Cuts at: 671 1625 5670 671
Size: 954 4045 1495
Fragments arranged by size:
4045 1495 954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2441 3544 3758 4060 4441 2441
Size: 1103 214 302 381 4494
Fragments arranged by size:
4494 1103 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4786 4840 6324 6489 50
Size: 4736 54 1484 165 55
Fragments arranged by size:
4736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4406 4406
Size: 6494
BsrFI r'CCGG_y
Cuts at: 364 4492 4673 4882 364
Size: 4128 181 209 1976
Fragments arranged by size:
4128 1976 209 181
BsrGI T'GTAC_A
Cuts at: 1370 2441 1370
Size: 1071 5423
BssSI C'ACGA_G
Cuts at: 4765 5123 4765
Size: 358 6136
BstAPI GCAn_nnn'nTGC
Cuts at: 2421 3871 2421
Size: 1450 5044
BstBI TT'CG_AA
Cuts at: 4855 4855
Size: 6494
BstEII G'GTnAC_C
Cuts at: 1908 1908
Size: 6494
BstYI r'GATC_y
Cuts at: 1 1717 2143 2651 3519 3986 4009 4344 4590
Size: 1716 426 508 868 467 23 335 246
Cuts at: 4590 5602 5688 5700 6046 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1716 1012 868 508 467 449 426 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2129 2129
Size: 6494
BtgI C'CryG_G
Cuts at: 773 3425 773
Size: 2652 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 192 2960 4444 4625 4788 192
Size: 2768 1484 181 163 1898
Fragments arranged by size:
2768 1898 1484 181 163
BtsI GCAGTG_nn'
Cuts at: 2364 3737 2364
Size: 1373 5121
DraI TTT'AAA
Cuts at: 942 3493 942
Size: 2551 3943
DraIII CAC_nnn'GTG
Cuts at: 1590 1666 1590
Size: 76 6418
DrdI GACnn_nn'nnGTC
Cuts at: 2312 4200 5058 2312
Size: 1888 858 3748
Fragments arranged by size:
3748 1888 858
EaeI y'GGCC_r
Cuts at: 351 1499 2187 3289 4079 4253 4644 4671 6016
Size: 1148 688 1102 790 174 391 27 1345
Cuts at: 6016 351
Size: 829
Fragments arranged by size:
1345 1148 1102 829 790 688 391 174
27
EagI C'GGCC_G
Cuts at: 1499 4079 1499
Size: 2580 3914
EarI CTCTTCn'nnn_
Cuts at: 242 1349 2072 4517 4727 242
Size: 1107 723 2445 210 2009
Fragments arranged by size:
2445 2009 1107 723 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2953 4702 5012 5158 6304 2953
Size: 1749 310 146 1146 3143
Fragments arranged by size:
3143 1749 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2700 3506 2700
Size: 806 5688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1297 1312 1333 1768 2057 2291 2731 2905
Size: 230 15 21 435 289 234 440 174
Cuts at: 2905 2943 3053 3373 4319 4751 5498 5766 1067
Size: 38 110 320 946 432 747 268 1795
Fragments arranged by size:
1795 946 747 440 435 432 320 289
268 234 230 174 110 38 21 15
EcoO109I rG'GnC_Cy
Cuts at: 414 532 533 2106 2813 2918 3633 414
Size: 118 1 1573 707 105 715 3275
Fragments arranged by size:
3275 1573 715 707 118 105 1
EcoRI G'AATT_C
Cuts at: 3498 3498
Size: 6494
EcoRV GAT'ATC
Cuts at: 1728 1728
Size: 6494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2887 4513 2887
Size: 1626 4868
FspI TGC'GCA
Cuts at: 4275 4275
Size: 6494
HaeII r_GCGC'y
Cuts at: 2966 3928 4176 5198 6177 2966
Size: 962 248 1022 979 3283
Fragments arranged by size:
3283 1022 979 962 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 191 223 385 417 790 822 1287 1319 1408
Size: 32 162 32 373 32 465 32 89
Cuts at: 1408 1440 2029 2061 2154 2186 2359 2391 2626
Size: 32 589 32 93 32 173 32 235
Cuts at: 2626 2658 2938 2970 3421 3453 4336 4366 4368
Size: 32 280 32 451 32 883 30 2
Cuts at: 4368 4398 191
Size: 30 2287
Fragments arranged by size:
2287 883 589 465 451 373 280 235
173 162 93 89 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 695 3216 3533 695
Size: 2521 317 3656
Fragments arranged by size:
3656 2521 317
KasI G'GCGC_C
Cuts at: 3924 4172 3924
Size: 248 6246
KpnI G_GTAC'C
Cuts at: 3514 3514
Size: 6494
MluI A'CGCG_T
Cuts at: 5927 5927
Size: 6494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 776 1217 2222 2871 3024 3182 5165 5349
Size: 339 441 1005 649 153 158 1983 184
Cuts at: 5349 437
Size: 1582
Fragments arranged by size:
1983 1582 1005 649 441 339 184 158
153
MscI TGG'CCA
Cuts at: 353 4255 353
Size: 3902 2592
MslI CAynn'nnrTG
Cuts at: 148 2725 3412 4483 4610 148
Size: 2577 687 1071 127 2032
Fragments arranged by size:
2577 2032 1071 687 127
MspA1I CmG'CkG
Cuts at: 2092 2521 3002 3605 3919 4279 5292 5537 2092
Size: 429 481 603 314 360 1013 245 3049
Fragments arranged by size:
3049 1013 603 481 429 360 314 245
NaeI GCC'GGC
Cuts at: 366 4675 366
Size: 4309 2185
NarI GG'CG_CC
Cuts at: 3925 4173 3925
Size: 248 6246
NcoI C'CATG_G
Cuts at: 3425 3425
Size: 6494
NgoMIV G'CCGG_C
Cuts at: 364 4673 364
Size: 4309 2185
NheI G'CTAG_C
Cuts at: 541 541
Size: 6494
NspI r_CATG'y
Cuts at: 153 3274 3547 4047 4954 153
Size: 3121 273 500 907 1693
Fragments arranged by size:
3121 1693 907 500 273
PasI CC'CwG_GG
Cuts at: 514 1263 3025 514
Size: 749 1762 3983
Fragments arranged by size:
3983 1762 749
PciI A'CATG_T
Cuts at: 3270 4950 3270
Size: 1680 4814
PflMI CCAn_nnn'nTGG
Cuts at: 3298 3298
Size: 6494
PfoI T'CCnGG_A
Cuts at: 1873 1873
Size: 6494
PmeI GTTT'AAAC
Cuts at: 3493 3493
Size: 6494
PmlI CAC'GTG
Cuts at: 180 180
Size: 6494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 5633 5665 244
Size: 32 5357 32 1073
Fragments arranged by size:
5357 1073 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2813 2106
Size: 707 5787
PshAI GACnn'nnGTC
Cuts at: 757 757
Size: 6494
PsiI TTA'TAA
Cuts at: 1546 1546
Size: 6494
PspOMI G'GGCC_C
Cuts at: 532 532
Size: 6494
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1727 1759 1727
Size: 32 6462
PstI C_TGCA'G
Cuts at: 1635 2498 3541 4226 1635
Size: 863 1043 685 3903
Fragments arranged by size:
3903 1043 863 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6494
PvuII CAG'CTG
Cuts at: 2092 2521 3919 4279 2092
Size: 429 1398 360 4307
Fragments arranged by size:
4307 1398 429 360
RsrII CG'GwC_CG
Cuts at: 4689 4689
Size: 6494
SacI G_AGCT'C
Cuts at: 2702 3508 2702
Size: 806 5688
SalI G'TCGA_C
Cuts at: 693 3531 693
Size: 2838 3656
SapI GCTCTTCn'nnn_
Cuts at: 4517 4727 4517
Size: 210 6284
SbfI CC_TGCA'GG
Cuts at: 3541 3541
Size: 6494
ScaI AGT'ACT
Cuts at: 271 6011 271
Size: 5740 754
SfcI C'TryA_G
Cuts at: 33 1137 1631 2433 2494 3537 4222 5215 5406
Size: 1104 494 802 61 1043 685 993 191
Cuts at: 5406 33
Size: 1121
Fragments arranged by size:
1121 1104 1043 993 802 685 494 191
61
SfoI GGC'GCC
Cuts at: 3926 4174 3926
Size: 248 6246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6494
SmaI CCC'GGG
Cuts at: 2923 3516 2923
Size: 593 5901
SmlI C'TyrA_G
Cuts at: 1438 1828 1842 2830 3649 4564 5056 5318 1438
Size: 390 14 988 819 915 492 262 2614
Fragments arranged by size:
2614 988 915 819 492 390 262 14
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6494
SphI G_CATG'C
Cuts at: 3547 4047 3547
Size: 500 5994
SspI AAT'ATT
Cuts at: 2600 3103 2600
Size: 503 5991
StuI AGG'CCT
Cuts at: 1289 2752 1289
Size: 1463 5031
StyI C'CwwG_G
Cuts at: 320 1617 2775 3206 3425 3628 3982 320
Size: 1297 1158 431 219 203 354 2832
Fragments arranged by size:
2832 1297 1158 431 354 219 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 712 1229 2565 3254 4077 4210 4669 4878 712
Size: 517 1336 689 823 133 459 209 2328
Fragments arranged by size:
2328 1336 823 689 517 459 209 133
TatI w'GTAC_w
Cuts at: 269 490 1370 2441 3186 6009 269
Size: 221 880 1071 745 2823 754
Fragments arranged by size:
2823 1071 880 754 745 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 389 421 1141 1173 1204 1236 6011 6043 389
Size: 32 720 32 31 32 4775 32 840
Fragments arranged by size:
4775 840 720 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 4291 4291
Size: 6494
XbaI T'CTAG_A
Cuts at: 3525 3525
Size: 6494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1591 3989 1591
Size: 2398 4096
XhoI C'TCGA_G
Cuts at: 2830 2830
Size: 6494
XmaI C'CCGG_G
Cuts at: 2921 3514 2921
Size: 593 5901
XmnI GAAnn'nnTTC
Cuts at: 1188 1188
Size: 6494
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AlfI AscI AvrII BaeI BcgI BfrBI
BglI BmgBI BsiWI BssHII BstXI BstZ17I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI ZraI