Restriction map for
pF1KA1739
(Circular) (Six-base) MAPSORT of: pF1KA1739.seq Check: 5171 from: 1 to: 5612
pF1KA1739 5612 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 852 1305 852
Size: 453 5159
AccI GT'mk_AC
Cuts at: 812 2650 812
Size: 1838 3774
Acc65I G'GTAC_C
Cuts at: 2628 2628
Size: 5612
AclI AA'CG_TT
Cuts at: 5452 5452
Size: 5612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 807 964 1730 2042 3437 3869 4222 4884
Size: 489 157 766 312 1395 432 353 662
Cuts at: 4884 318
Size: 1046
Fragments arranged by size:
1395 1046 766 662 489 432 353 312
157
AflII C'TTAA_G
Cuts at: 2335 2335
Size: 5612
AflIII A'CryG_T
Cuts at: 1076 2964 4764 5045 1076
Size: 1888 1800 281 1643
Fragments arranged by size:
1888 1800 1643 281
AleI CACnn'nnGTG
Cuts at: 1807 1807
Size: 5612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1570 1570
Size: 5612
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 662
Size: 32 5580
AlwNI CAG_nnn'CTG
Cuts at: 1682 1941 2415 2574 2917 4355 1682
Size: 259 474 159 343 1438 2939
Fragments arranged by size:
2939 1438 474 343 259 159
ApaI G_GGCC'C
Cuts at: 698 1138 1475 2079 2381 698
Size: 440 337 604 302 3929
Fragments arranged by size:
3929 604 440 337 302
ApaLI G'TGCA_C
Cuts at: 516 4450 516
Size: 3934 1678
ApoI r'AATT_y
Cuts at: 2616 4965 5221 2616
Size: 2349 256 3007
Fragments arranged by size:
3007 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5612
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
AvaI C'yCGr_G
Cuts at: 314 1643 1906 2072 2512 2590 2632 314
Size: 1329 263 166 440 78 42 3294
Fragments arranged by size:
3294 1329 440 263 166 78 42
BamHI G'GATC_C
Cuts at: 2301 2637 2301
Size: 336 5276
BanI G'GyrC_C
Cuts at: 310 493 1235 1357 2360 2516 2628 3042 3290
Size: 183 742 122 1003 156 112 414 248
Cuts at: 3290 3325 310
Size: 35 2597
Fragments arranged by size:
2597 1003 742 414 248 183 156 122
112 35
BanII G_rGCy'C
Cuts at: 290 698 1138 1475 1520 2079 2252 2381 2513
Size: 408 440 337 45 559 173 129 132
Cuts at: 2513 2534 2626 3656 290
Size: 21 92 1030 2246
Fragments arranged by size:
2246 1030 559 440 408 337 173 132
129 92 45 21
BbeI G_GCGC'C
Cuts at: 314 1239 3046 3294 314
Size: 925 1807 248 2632
Fragments arranged by size:
2632 1807 925 248
BbsI GAAGACnn'nnnn_
Cuts at: 200 400 718 200
Size: 200 318 5094
Fragments arranged by size:
5094 318 200
BbvCI CC'TCA_GC
Cuts at: 170 170
Size: 5612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 835 869 2036 2070 835
Size: 34 1167 34 4377
Fragments arranged by size:
4377 1167 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 415 3510 4566 5612 415
Size: 3095 1056 1046 415
Fragments arranged by size:
3095 1056 1046 415
BclI T'GATC_A
Cuts at: 3132 3132
Size: 5612
BglI GCCn_nnn'nGGC
Cuts at: 294 319 611 2125 294
Size: 25 292 1514 3781
Fragments arranged by size:
3781 1514 292 25
BglII A'GATC_T
Cuts at: 3127 5164 3127
Size: 2037 3575
BlpI GC'TnA_GC
Cuts at: 2724 2724
Size: 5612
Bme1580I G_kGCm'C
Cuts at: 520 667 698 1138 1360 1475 1819 2079 2381
Size: 147 31 440 222 115 344 260 302
Cuts at: 2381 2519 3237 3330 4454 520
Size: 138 718 93 1124 1678
Fragments arranged by size:
1678 1124 718 440 344 302 260 222
147 138 115 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 129 302 2824 2905 3240 5384 129
Size: 173 2522 81 335 2144 357
Fragments arranged by size:
2522 2144 357 335 173 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 654 3979 654
Size: 3325 2287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1279 1683 1918 2209 2254 2378 370
Size: 909 404 235 291 45 124 3604
Fragments arranged by size:
3604 909 404 291 235 124 45
Bpu10I CC'TnA_GC
Cuts at: 170 599 2528 170
Size: 429 1929 3254
Fragments arranged by size:
3254 1929 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 2788 3667 4381 4679 1438
Size: 1350 879 714 298 2371
Fragments arranged by size:
2371 1350 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5316 5316
Size: 5612
BsaAI yAC'GTr
Cuts at: 306 2475 2965 306
Size: 2169 490 2953
Fragments arranged by size:
2953 2169 490
BsaBI GATnn'nnATC
Cuts at: 6 3126 5163 6
Size: 3120 2037 455
Fragments arranged by size:
3120 2037 455
BsaHI Gr'CG_yC
Cuts at: 311 1236 3043 3291 3993 5489 311
Size: 925 1807 248 702 1496 434
Fragments arranged by size:
1807 1496 925 702 434 248
BsaWI w'CCGG_w
Cuts at: 2801 3322 4000 4411 4558 4832 2801
Size: 521 678 411 147 274 3581
Fragments arranged by size:
3581 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 457 487 1237 1267 1831 1861 457
Size: 30 750 30 564 30 4208
Fragments arranged by size:
4208 750 564 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1324 1507 1836 2011 2608 1324
Size: 183 329 175 597 4328
Fragments arranged by size:
4328 597 329 183 175
BseYI C'CCAG_C
Cuts at: 594 1195 1958 3037 3795 4460 4868 594
Size: 601 763 1079 758 665 408 1338
Fragments arranged by size:
1338 1079 763 758 665 601 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 1646 1606
Size: 40 5572
BsiEI CG_ry'CG
Cuts at: 104 1386 3154 3200 4430 104
Size: 1282 1768 46 1230 1286
Fragments arranged by size:
1768 1286 1282 1230 46
BsiHKAI G_wGCw'C
Cuts at: 520 2534 2626 3404 3594 4454 520
Size: 2014 92 778 190 860 1678
Fragments arranged by size:
2014 1678 860 778 190 92
BsmBI CGTCTCn'nnnn_
Cuts at: 1433 2541 1433
Size: 1108 4504
Bsp1286I G_dGCh'C
Cuts at: 290 520 667 698 1138 1360 1475 1520 1819
Size: 230 147 31 440 222 115 45 299
Cuts at: 1819 2079 2252 2381 2513 2519 2534 2626 3237
Size: 260 173 129 132 6 15 92 611
Cuts at: 3237 3330 3404 3594 3656 4454 290
Size: 93 74 190 62 798 1448
Fragments arranged by size:
1448 798 611 440 299 260 230 222
190 173 147 132 129 115 93 92
74 62 45 31 15 6
BspHI T'CATG_A
Cuts at: 4044 4044
Size: 5612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 852 1305 1898 2662 2876 3178 3559 852
Size: 453 593 764 214 302 381 2905
Fragments arranged by size:
2905 764 593 453 381 302 214
BsrBI CCG'CTC
Cuts at: 50 831 1574 3904 3958 5442 5607 50
Size: 781 743 2330 54 1484 165 55
Fragments arranged by size:
2330 1484 781 743 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2161 3524 2161
Size: 1363 4249
BsrFI r'CCGG_y
Cuts at: 250 1564 2168 3610 3791 250
Size: 1314 604 1442 181 2071
Fragments arranged by size:
2071 1442 1314 604 181
BssSI C'ACGA_G
Cuts at: 872 1212 1345 3883 4591 872
Size: 340 133 2538 708 1893
Fragments arranged by size:
2538 1893 708 340 133
BstAPI GCAn_nnn'nTGC
Cuts at: 964 1005 1014 2523 2989 964
Size: 41 9 1509 466 3587
Fragments arranged by size:
3587 1509 466 41 9
BstBI TT'CG_AA
Cuts at: 3973 3973
Size: 5612
BstEII G'GTnAC_C
Cuts at: 2058 2058
Size: 5612
BstXI CCAn_nnnn'nTGG
Cuts at: 1257 2371 1257
Size: 1114 4498
BstYI r'GATC_y
Cuts at: 1 1115 1708 2301 2637 3104 3127 3462 3708
Size: 1114 593 593 336 467 23 335 246
Cuts at: 3708 4014 4026 4112 5164 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1114 1052 593 593 467 449 336 335
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 224 383 1239 1595 1691 1764 107
Size: 117 159 856 356 96 73 3955
Fragments arranged by size:
3955 856 356 159 117 96 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1018 3562 3743 3906 1018
Size: 2544 181 163 2724
Fragments arranged by size:
2724 2544 181 163
BtsI GCAGTG_nn'
Cuts at: 842 1983 2572 2855 842
Size: 1141 589 283 3599
Fragments arranged by size:
3599 1141 589 283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1029 1048 1064 1083 1746 1781 1029
Size: 19 16 19 663 35 4860
Fragments arranged by size:
4860 663 35 19 19 16
DraI TTT'AAA
Cuts at: 2611 2611
Size: 5612
DraIII CAC_nnn'GTG
Cuts at: 1377 1377
Size: 5612
DrdI GACnn_nn'nnGTC
Cuts at: 808 3318 4662 808
Size: 2510 1344 1758
Fragments arranged by size:
2510 1758 1344
EaeI y'GGCC_r
Cuts at: 221 641 1088 1379 1598 1688 1698 3197 3371
Size: 420 447 291 219 90 10 1499 174
Cuts at: 3371 3762 3789 5134 221
Size: 391 27 1345 699
Fragments arranged by size:
1499 1345 699 447 420 391 291 219
174 90 27 10
EagI C'GGCC_G
Cuts at: 3197 3197
Size: 5612
EarI CTCTTCn'nnn_
Cuts at: 1106 2177 3635 3845 1106
Size: 1071 1458 210 2873
Fragments arranged by size:
2873 1458 1071 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2466 3820 4562 4708 5422 2466
Size: 1354 742 146 714 2656
Fragments arranged by size:
2656 1354 742 714 146
EcoICRI GAG'CTC
Cuts at: 2532 2624 2532
Size: 92 5520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 370 807 964 1279 1683 1730 1918 2042
Size: 52 437 157 315 404 47 188 124
Cuts at: 2042 2209 2254 2378 3437 3869 4222 4884 318
Size: 167 45 124 1059 432 353 662 1046
Fragments arranged by size:
1059 1046 662 437 432 404 353 315
188 167 157 124 124 52 47 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1489 1489
Size: 5612
EcoO109I rG'GnC_Cy
Cuts at: 153 321 345 378 694 695 1134 1135 1321
Size: 168 24 33 316 1 439 1 186
Cuts at: 1321 1471 1472 1647 1887 1910 1938 1976 2076
Size: 150 1 175 240 23 28 38 100
Cuts at: 2076 2216 2377 2378 2751 153
Size: 140 161 1 373 3014
Fragments arranged by size:
3014 439 373 316 240 186 175 168
161 150 140 100 38 33 28 24
23 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2616 2616
Size: 5612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 831 1575 2047 3631 831
Size: 744 472 1584 2812
Fragments arranged by size:
2812 1584 744 472
FspI TGC'GCA
Cuts at: 3393 3393
Size: 5612
HaeII r_GCGC'y
Cuts at: 314 1239 3046 3294 4524 5295 314
Size: 925 1807 248 1230 771 631
Fragments arranged by size:
1807 1230 925 771 631 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2584 2616 3454 3484 3486 3516 2584
Size: 32 838 30 2 30 4680
Fragments arranged by size:
4680 838 32 30 30 2
HincII GTy'rAC
Cuts at: 528 1671 2651 528
Size: 1143 980 3489
Fragments arranged by size:
3489 1143 980
KasI G'GCGC_C
Cuts at: 310 1235 3042 3290 310
Size: 925 1807 248 2632
Fragments arranged by size:
2632 1807 925 248
KpnI G_GTAC'C
Cuts at: 2632 2632
Size: 5612
MluI A'CGCG_T
Cuts at: 5045 5045
Size: 5612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 869 1088 1874 2449 2609 4371 4555 869
Size: 219 786 575 160 1762 184 1926
Fragments arranged by size:
1926 1762 786 575 219 184 160
MscI TGG'CCA
Cuts at: 223 1700 3373 223
Size: 1477 1673 2462
Fragments arranged by size:
2462 1673 1477
MslI CAynn'nnrTG
Cuts at: 1804 1807 3601 3728 1804
Size: 3 1794 127 3688
Fragments arranged by size:
3688 1794 127 3
MspA1I CmG'CkG
Cuts at: 996 1122 1302 1503 1581 1590 1682 1868 2723
Size: 126 180 201 78 9 92 186 855
Cuts at: 2723 3037 3397 4181 4426 996
Size: 314 360 784 245 2182
Fragments arranged by size:
2182 855 784 360 314 245 201 186
180 126 92 78 9
NaeI GCC'GGC
Cuts at: 1566 2170 3793 1566
Size: 604 1623 3385
Fragments arranged by size:
3385 1623 604
NarI GG'CG_CC
Cuts at: 311 1236 3043 3291 311
Size: 925 1807 248 2632
Fragments arranged by size:
2632 1807 925 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5612
NgoMIV G'CCGG_C
Cuts at: 1564 2168 3791 1564
Size: 604 1623 3385
Fragments arranged by size:
3385 1623 604
NspI r_CATG'y
Cuts at: 534 962 1080 1729 2356 2665 3165 4768 534
Size: 428 118 649 627 309 500 1603 1378
Fragments arranged by size:
1603 1378 649 627 500 428 309 118
PasI CC'CwG_GG
Cuts at: 1475 2121 1475
Size: 646 4966
PciI A'CATG_T
Cuts at: 1076 4764 1076
Size: 3688 1924
PflMI CCAn_nnn'nTGG
Cuts at: 1377 1941 1377
Size: 564 5048
PfoI T'CCnGG_A
Cuts at: 257 257
Size: 5612
PmeI GTTT'AAAC
Cuts at: 2611 2611
Size: 5612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 5580
PpuMI rG'GwC_Cy
Cuts at: 1647 1887 1910 2216 1647
Size: 240 23 306 5043
Fragments arranged by size:
5043 306 240 23
PspOMI G'GGCC_C
Cuts at: 694 1134 1471 2075 2377 694
Size: 440 337 604 302 3929
Fragments arranged by size:
3929 604 440 337 302
PstI C_TGCA'G
Cuts at: 461 923 1001 1019 1502 2561 2659 3344 461
Size: 462 78 18 483 1059 98 685 2729
Fragments arranged by size:
2729 1059 685 483 462 98 78 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5612
PvuII CAG'CTG
Cuts at: 996 1503 1581 1590 3037 3397 996
Size: 507 78 9 1447 360 3211
Fragments arranged by size:
3211 1447 507 360 78 9
RsrII CG'GwC_CG
Cuts at: 3807 3807
Size: 5612
SacI G_AGCT'C
Cuts at: 2534 2626 2534
Size: 92 5520
SalI G'TCGA_C
Cuts at: 2649 2649
Size: 5612
SanDI GG'GwC_CC
Cuts at: 1647 1647
Size: 5612
SapI GCTCTTCn'nnn_
Cuts at: 2177 3635 3845 2177
Size: 1458 210 3944
Fragments arranged by size:
3944 1458 210
SbfI CC_TGCA'GG
Cuts at: 1019 2659 1019
Size: 1640 3972
ScaI AGT'ACT
Cuts at: 5129 5129
Size: 5612
SexAI A'CCwGG_T
Cuts at: 1373 1823 1373
Size: 450 5162
SfcI C'TryA_G
Cuts at: 33 457 919 997 1015 1498 2557 2655 3340
Size: 424 462 78 18 483 1059 98 685
Cuts at: 3340 4308 4499 33
Size: 968 191 1146
Fragments arranged by size:
1146 1059 968 685 483 462 424 191
98 78 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 611 611
Size: 5612
SfoI GGC'GCC
Cuts at: 312 1237 3044 3292 312
Size: 925 1807 248 2632
Fragments arranged by size:
2632 1807 925 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
SgrAI Cr'CCGG_yG
Cuts at: 2168 2168
Size: 5612
SmaI CCC'GGG
Cuts at: 1645 1908 2074 2634 1645
Size: 263 166 560 4623
Fragments arranged by size:
4623 560 263 166
SmlI C'TyrA_G
Cuts at: 1453 2335 2767 3682 4396 4658 1453
Size: 882 432 915 714 262 2407
Fragments arranged by size:
2407 915 882 714 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5612
SphI G_CATG'C
Cuts at: 962 2356 2665 3165 962
Size: 1394 309 500 3409
Fragments arranged by size:
3409 1394 500 309
SrfI GCCC'GGGC
Cuts at: 2074 2074
Size: 5612
StyI C'CwwG_G
Cuts at: 107 2063 2201 2219 2243 2746 3100 107
Size: 1956 138 18 24 503 354 2619
Fragments arranged by size:
2619 1956 503 354 138 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 1384 1684 3195 3328 3787 3996 445
Size: 939 300 1511 133 459 209 2061
Fragments arranged by size:
2061 1511 939 459 300 209 133
TatI w'GTAC_w
Cuts at: 635 5127 635
Size: 4492 1120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1831 1863 5129 5161 1831
Size: 32 3266 32 2282
Fragments arranged by size:
3266 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 204 808 1633 3409 204
Size: 604 825 1776 2407
Fragments arranged by size:
2407 1776 825 604
XbaI T'CTAG_A
Cuts at: 2643 2643
Size: 5612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1527 1683 3107 1527
Size: 156 1424 4032
Fragments arranged by size:
4032 1424 156
XmaI C'CCGG_G
Cuts at: 1643 1906 2072 2632 1643
Size: 263 166 560 4623
Fragments arranged by size:
4623 560 263 166
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BsmI BspEI BsrGI BssHII BstZ17I
Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SnaBI SspI StuI SwaI
XhoI XmnI ZraI