Restriction map for
pF1KA1741
(Circular) (Six-base) MAPSORT of: pF1KA1741.seq Check: 8946 from: 1 to: 8300
pF1KA1741 8300 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2474 4421 4564 2474
Size: 1947 143 6210
Fragments arranged by size:
6210 1947 143
AccI GT'mk_AC
Cuts at: 118 5338 118
Size: 5220 3080
Acc65I G'GTAC_C
Cuts at: 2728 4882 5316 2728
Size: 2154 434 5712
Fragments arranged by size:
5712 2154 434
AclI AA'CG_TT
Cuts at: 8140 8140
Size: 8300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 741 1097 1415 1631 2248 3770 4673 4952 5095
Size: 356 318 216 617 1522 903 279 143
Cuts at: 5095 5299 6125 6557 6910 7572 741
Size: 204 826 432 353 662 1469
Fragments arranged by size:
1522 1469 903 826 662 617 432 356
353 318 279 216 204 143
AfeI AGC'GCT
Cuts at: 571 2973 571
Size: 2402 5898
AflIII A'CryG_T
Cuts at: 5652 7452 7733 5652
Size: 1800 281 6219
Fragments arranged by size:
6219 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2238 2238
Size: 8300
AleI CACnn'nnGTG
Cuts at: 586 586
Size: 8300
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 2626
Size: 32 8268
AlwNI CAG_nnn'CTG
Cuts at: 1003 1059 1987 2037 2140 4090 4384 4470 4579
Size: 56 928 50 103 1950 294 86 109
Cuts at: 4579 5605 7043 1003
Size: 1026 1438 2260
Fragments arranged by size:
2260 1950 1438 1026 928 294 109 103
86 56 50
ApaI G_GGCC'C
Cuts at: 246 3319 4863 246
Size: 3073 1544 3683
Fragments arranged by size:
3683 3073 1544
ApaLI G'TGCA_C
Cuts at: 7138 7138
Size: 8300
ApoI r'AATT_y
Cuts at: 2299 2704 2767 3097 5304 7653 7909 2299
Size: 405 63 330 2207 2349 256 2690
Fragments arranged by size:
2690 2349 2207 405 330 256 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 8300
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8300
AvaI C'yCGr_G
Cuts at: 158 209 239 311 1167 1560 2069 2125 3070
Size: 51 30 72 856 393 509 56 945
Cuts at: 3070 3226 4533 4955 5320 158
Size: 156 1307 422 365 3138
Fragments arranged by size:
3138 1307 945 856 509 422 393 365
156 72 56 51 30
BamHI G'GATC_C
Cuts at: 3175 5325 3175
Size: 2150 6150
BanI G'GyrC_C
Cuts at: 180 288 2728 2920 4152 4433 4675 4853 4882
Size: 108 2440 192 1232 281 242 178 29
Cuts at: 4882 4959 5023 5316 5730 5978 6013 180
Size: 77 64 293 414 248 35 2467
Fragments arranged by size:
2467 2440 1232 414 293 281 248 242
192 178 108 77 64 35 29
BanII G_rGCy'C
Cuts at: 246 1150 1175 1191 1238 1444 1820 1892 3058
Size: 904 25 16 47 206 376 72 1166
Cuts at: 3058 3312 3319 3649 4172 4182 4235 4687 4863
Size: 254 7 330 523 10 53 452 176
Cuts at: 4863 5065 5314 6344 246
Size: 202 249 1030 2202
Fragments arranged by size:
2202 1166 1030 904 523 452 376 330
254 249 206 202 176 72 53 47
25 16 10 7
BbeI G_GCGC'C
Cuts at: 4156 5734 5982 4156
Size: 1578 248 6474
Fragments arranged by size:
6474 1578 248
BbsI GAAGACnn'nnnn_
Cuts at: 3131 3571 4349 4670 3131
Size: 440 778 321 6761
Fragments arranged by size:
6761 778 440 321
BbvCI CC'TCA_GC
Cuts at: 1637 3824 4408 5067 5108 1637
Size: 2187 584 659 41 4829
Fragments arranged by size:
4829 2187 659 584 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 869 903 4892 4926 869
Size: 34 3989 34 4243
Fragments arranged by size:
4243 3989 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6198 7254 8300 6198
Size: 1056 1046 6198
Fragments arranged by size:
6198 1056 1046
BclI T'GATC_A
Cuts at: 5820 5820
Size: 8300
BglI GCCn_nnn'nGGC
Cuts at: 297 1186 1621 1630 3985 4544 5077 5259 297
Size: 889 435 9 2355 559 533 182 3338
Fragments arranged by size:
3338 2355 889 559 533 435 182 9
BglII A'GATC_T
Cuts at: 2286 5815 7852 2286
Size: 3529 2037 2734
Fragments arranged by size:
3529 2734 2037
BlpI GC'TnA_GC
Cuts at: 135 2222 5103 5412 135
Size: 2087 2881 309 3023
Fragments arranged by size:
3023 2881 2087 309
Bme1580I G_kGCm'C
Cuts at: 246 1840 2668 3225 3319 4228 4438 4856 4863
Size: 1594 828 557 94 909 210 418 7
Cuts at: 4863 5026 5925 6018 7142 246
Size: 163 899 93 1124 1404
Fragments arranged by size:
1594 1404 1124 909 899 828 557 418
210 163 94 93 7
BmgBI CAC'GTC
Cuts at: 4668 4668
Size: 8300
BmrI ACTGGGnnnn_n'
Cuts at: 467 1409 2183 2646 2734 2772 2898 4047 4760
Size: 942 774 463 88 38 126 1149 713
Cuts at: 4760 5200 5512 5593 5928 8072 467
Size: 440 312 81 335 2144 695
Fragments arranged by size:
2144 1149 942 774 713 695 463 440
335 312 126 88 81 38
BmtI G_CTAG'C
Cuts at: 3269 3269
Size: 8300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1808 2801 4423 5247 6667 1808
Size: 993 1622 824 1420 3441
Fragments arranged by size:
3441 1622 1420 993 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 776 1175 1390 1536 1972 2117 2237 2658
Size: 321 399 215 146 436 145 120 421
Cuts at: 2658 2677 2991 3453 4022 4112 4291 4495 4618
Size: 19 314 462 569 90 179 204 123
Cuts at: 4618 4647 455
Size: 29 4108
Fragments arranged by size:
4108 569 462 436 421 399 321 314
215 204 179 146 145 123 120 90
29 19
Bpu10I CC'TnA_GC
Cuts at: 875 996 1637 2021 3052 3311 3700 3824 4408
Size: 121 641 384 1031 259 389 124 584
Cuts at: 4408 5059 5067 5108 875
Size: 651 8 41 4067
Fragments arranged by size:
4067 1031 651 641 584 389 384 259
124 121 41 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 1834 2087 3726 5476 6355 7069 7367 912
Size: 922 253 1639 1750 879 714 298 1845
Fragments arranged by size:
1845 1750 1639 922 879 714 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 2071 2309 2396 2416 3039 3230 3610 4274 4324
Size: 238 87 20 623 191 380 664 50
Cuts at: 4324 8004 2071
Size: 3680 2367
Fragments arranged by size:
3680 2367 664 623 380 238 191 87
50 20
BsaAI yAC'GTr
Cuts at: 3210 5653 3210
Size: 2443 5857
BsaBI GATnn'nnATC
Cuts at: 6 5814 7851 6
Size: 5808 2037 455
Fragments arranged by size:
5808 2037 455
BsaHI Gr'CG_yC
Cuts at: 3020 4153 5731 5979 6681 8177 3020
Size: 1133 1578 248 702 1496 3143
Fragments arranged by size:
3143 1578 1496 1133 702 248
BsaWI w'CCGG_w
Cuts at: 5489 6010 6688 7099 7246 7520 5489
Size: 521 678 411 147 274 6269
Fragments arranged by size:
6269 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 711 741 1132 1162 5196 5226 711
Size: 30 391 30 4034 30 3785
Fragments arranged by size:
4034 3785 391 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 187 452 481 496 844 849 933 1348
Size: 3 265 29 15 348 5 84 415
Cuts at: 1348 1351 1882 2062 2065 2414 2518 3480 3854
Size: 3 531 180 3 349 104 962 374
Cuts at: 3854 4341 4492 4570 4603 5125 184
Size: 487 151 78 33 522 3359
Fragments arranged by size:
3359 962 531 522 487 415 374 349
348 265 180 151 104 84 78 33
29 15 5 3 3 3
BseYI C'CCAG_C
Cuts at: 277 368 472 504 558 1162 1207 1434 1699
Size: 91 104 32 54 604 45 227 265
Cuts at: 1699 1800 2326 2594 3080 3362 3395 3600 4302
Size: 101 526 268 486 282 33 205 702
Cuts at: 4302 5725 6483 7148 7556 277
Size: 1423 758 665 408 1021
Fragments arranged by size:
1423 1021 758 702 665 604 526 486
408 282 268 265 227 205 104 101
91 54 45 33 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1148 1756 536
Size: 612 608 7080
Fragments arranged by size:
7080 612 608
BsiEI CG_ry'CG
Cuts at: 104 648 5842 5888 7118 104
Size: 544 5194 46 1230 1286
Fragments arranged by size:
5194 1286 1230 544 46
BsiHKAI G_wGCw'C
Cuts at: 815 1406 2467 2561 3058 4172 5314 6092 6282
Size: 591 1061 94 497 1114 1142 778 190
Cuts at: 6282 7142 815
Size: 860 1973
Fragments arranged by size:
1973 1142 1114 1061 860 778 591 497
190 94
Bsp1286I G_dGCh'C
Cuts at: 246 815 1150 1175 1191 1238 1406 1444 1820
Size: 569 335 25 16 47 168 38 376
Cuts at: 1820 1840 1892 2467 2561 2668 3058 3225 3312
Size: 20 52 575 94 107 390 167 87
Cuts at: 3312 3319 3649 4172 4182 4228 4235 4438 4687
Size: 7 330 523 10 46 7 203 249
Cuts at: 4687 4856 4863 5026 5065 5314 5925 6018 6092
Size: 169 7 163 39 249 611 93 74
Cuts at: 6092 6282 6344 7142 246
Size: 190 62 798 1404
Fragments arranged by size:
1404 798 611 575 569 523 390 376
335 330 249 249 203 190 169 168
167 163 107 94 93 87 74 62
52 47 46 39 38 25 20 16
10 7 7 7
BspHI T'CATG_A
Cuts at: 6732 6732
Size: 8300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 906 2260 2474 4421 4564 5350 5564 5866 6247
Size: 1354 214 1947 143 786 214 302 381
Cuts at: 6247 906
Size: 2959
Fragments arranged by size:
2959 1947 1354 786 381 302 214 214
143
BsrBI CCG'CTC
Cuts at: 50 6592 6646 8130 8295 50
Size: 6542 54 1484 165 55
Fragments arranged by size:
6542 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6212 6212
Size: 8300
BsrFI r'CCGG_y
Cuts at: 3158 3724 6298 6479 3158
Size: 566 2574 181 4979
Fragments arranged by size:
4979 2574 566 181
BssSI C'ACGA_G
Cuts at: 1551 6571 7279 1551
Size: 5020 708 2572
Fragments arranged by size:
5020 2572 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4579 5677 4579
Size: 1098 7202
BstBI TT'CG_AA
Cuts at: 1535 5167 6661 1535
Size: 3632 1494 3174
Fragments arranged by size:
3632 3174 1494
BstEII G'GTnAC_C
Cuts at: 4071 4071
Size: 8300
BstXI CCAn_nnnn'nTGG
Cuts at: 285 376 457 588 1374 3633 4302 4509 4625
Size: 91 81 131 786 2259 669 207 116
Cuts at: 4625 285
Size: 3960
Fragments arranged by size:
3960 2259 786 669 207 131 116 91
81
BstYI r'GATC_y
Cuts at: 1 596 1302 2286 3175 4759 4799 5325 5792
Size: 595 706 984 889 1584 40 526 467
Cuts at: 5792 5815 6150 6396 6702 6714 6800 7852 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1584 1052 984 889 706 595 526 467
449 335 306 246 86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 827 990 1223 4057 4240 5232 827
Size: 163 233 2834 183 992 3895
Fragments arranged by size:
3895 2834 992 233 183 163
BtgI C'CryG_G
Cuts at: 140 584 2850 3671 4945 5153 140
Size: 444 2266 821 1274 208 3287
Fragments arranged by size:
3287 2266 1274 821 444 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1790 6250 6431 6594 1790
Size: 4460 181 163 3496
Fragments arranged by size:
4460 3496 181 163
BtsI GCAGTG_nn'
Cuts at: 151 722 2035 3000 3200 3330 5543 151
Size: 571 1313 965 200 130 2213 2908
Fragments arranged by size:
2908 2213 1313 965 571 200 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 476 511 476
Size: 35 8265
DraI TTT'AAA
Cuts at: 5299 5299
Size: 8300
DraIII CAC_nnn'GTG
Cuts at: 2191 3552 2191
Size: 1361 6939
DrdI GACnn_nn'nnGTC
Cuts at: 3885 5013 6006 7350 3885
Size: 1128 993 1344 4835
Fragments arranged by size:
4835 1344 1128 993
EaeI y'GGCC_r
Cuts at: 578 602 1652 2496 3156 3160 5885 6059 6450
Size: 24 1050 844 660 4 2725 174 391
Cuts at: 6450 6477 7822 578
Size: 27 1345 1056
Fragments arranged by size:
2725 1345 1056 1050 844 660 391 174
27 24 4
EagI C'GGCC_G
Cuts at: 5885 5885
Size: 8300
EarI CTCTTCn'nnn_
Cuts at: 587 760 803 2770 2833 2896 2962 3746 3802
Size: 173 43 1967 63 63 66 784 56
Cuts at: 3802 4692 4970 6323 6533 587
Size: 890 278 1353 210 2354
Fragments arranged by size:
2354 1967 1353 890 784 278 210 173
66 63 63 56 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 544 1351 6508 7250 7396 8110 544
Size: 807 5157 742 146 714 734
Fragments arranged by size:
5157 807 742 734 714 146
EcoICRI GAG'CTC
Cuts at: 3056 4170 5312 3056
Size: 1114 1142 6044
Fragments arranged by size:
6044 1142 1114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 741 776 1097 1175 1390 1415 1536 1631
Size: 286 35 321 78 215 25 121 95
Cuts at: 1631 1972 2117 2237 2248 2658 2677 2991 3453
Size: 341 145 120 11 410 19 314 462
Cuts at: 3453 3770 4022 4112 4291 4495 4618 4647 4673
Size: 317 252 90 179 204 123 29 26
Cuts at: 4673 4952 5095 5299 6125 6557 6910 7572 455
Size: 279 143 204 826 432 353 662 1183
Fragments arranged by size:
1183 826 662 462 432 410 353 341
321 317 314 286 279 252 215 204
204 179 145 143 123 121 120 95
90 78 35 29 26 25 19 11
EcoNI CCTnn'n_nnAGG
Cuts at: 874 1282 1966 3136 4474 4552 4626 4897 4927
Size: 408 684 1170 1338 78 74 271 30
Cuts at: 4927 874
Size: 4247
Fragments arranged by size:
4247 1338 1170 684 408 271 78 74
30
EcoO109I rG'GnC_Cy
Cuts at: 243 316 364 540 630 707 867 958 1000
Size: 73 48 176 90 77 160 91 42
Cuts at: 1000 1197 1556 2137 3011 3315 3316 4467 4545
Size: 197 359 581 874 304 1 1151 78
Cuts at: 4545 4747 4860 5181 5439 243
Size: 202 113 321 258 3104
Fragments arranged by size:
3104 1151 874 581 359 321 304 258
202 197 176 160 113 91 90 78
77 73 48 42 1
EcoRI G'AATT_C
Cuts at: 2704 5304 2704
Size: 2600 5700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3148 6319 3148
Size: 3171 5129
FseI GG_CCGG'CC
Cuts at: 3162 3162
Size: 8300
FspI TGC'GCA
Cuts at: 6081 6081
Size: 8300
HaeII r_GCGC'y
Cuts at: 393 573 1386 2975 4156 5734 5982 7212 7983
Size: 180 813 1589 1181 1578 248 1230 771
Cuts at: 7983 393
Size: 710
Fragments arranged by size:
1589 1578 1230 1181 813 771 710 248
180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 673 705 711 743 1132 1164 2085 2117 2224
Size: 32 6 32 389 32 921 32 107
Cuts at: 2224 2256 3160 3192 3350 3382 3456 3488 4072
Size: 32 904 32 158 32 74 32 584
Cuts at: 4072 4104 4336 4368 5119 5151 6142 6172 6174
Size: 32 232 32 751 32 991 30 2
Cuts at: 6174 6204 673
Size: 30 2769
Fragments arranged by size:
2769 991 921 904 751 584 389 232
158 107 74 32 32 32 32 32
32 32 32 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 1789 5043 5339 1789
Size: 3254 296 4750
Fragments arranged by size:
4750 3254 296
HindIII A'AGCT_T
Cuts at: 1079 3033 1079
Size: 1954 6346
KasI G'GCGC_C
Cuts at: 4152 5730 5978 4152
Size: 1578 248 6474
Fragments arranged by size:
6474 1578 248
KpnI G_GTAC'C
Cuts at: 2732 4886 5320 2732
Size: 2154 434 5712
Fragments arranged by size:
5712 2154 434
MluI A'CGCG_T
Cuts at: 7733 7733
Size: 8300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 872 1535 2562 3915 4532 4877 7059 7243 872
Size: 663 1027 1353 617 345 2182 184 1929
Fragments arranged by size:
2182 1929 1353 1027 663 617 345 184
MscI TGG'CCA
Cuts at: 604 6061 604
Size: 5457 2843
MslI CAynn'nnrTG
Cuts at: 112 586 6289 6416 112
Size: 474 5703 127 1996
Fragments arranged by size:
5703 1996 474 127
MspA1I CmG'CkG
Cuts at: 1105 1804 2156 2348 2610 3248 3574 3600 3629
Size: 699 352 192 262 638 326 26 29
Cuts at: 3629 4528 5107 5155 5411 5725 6085 6869 7114
Size: 899 579 48 256 314 360 784 245
Cuts at: 7114 1105
Size: 2291
Fragments arranged by size:
2291 899 784 699 638 579 360 352
326 314 262 256 245 192 48 29
26
NaeI GCC'GGC
Cuts at: 3160 6481 3160
Size: 3321 4979
NarI GG'CG_CC
Cuts at: 4153 5731 5979 4153
Size: 1578 248 6474
Fragments arranged by size:
6474 1578 248
NcoI C'CATG_G
Cuts at: 140 2850 140
Size: 2710 5590
NgoMIV G'CCGG_C
Cuts at: 3158 6479 3158
Size: 3321 4979
NheI G'CTAG_C
Cuts at: 3265 3265
Size: 8300
NruI TCG'CGA
Cuts at: 693 693
Size: 8300
NspI r_CATG'y
Cuts at: 357 1758 5353 5853 7456 357
Size: 1401 3595 500 1603 1201
Fragments arranged by size:
3595 1603 1401 1201 500
PasI CC'CwG_GG
Cuts at: 523 731 2551 2661 3418 3982 4335 4464 523
Size: 208 1820 110 757 564 353 129 4359
Fragments arranged by size:
4359 1820 757 564 353 208 129 110
PciI A'CATG_T
Cuts at: 7452 7452
Size: 8300
PflMI CCAn_nnn'nTGG
Cuts at: 917 1245 2191 2474 2832 3276 4090 4149 4164
Size: 328 946 283 358 444 814 59 15
Cuts at: 4164 917
Size: 5053
Fragments arranged by size:
5053 946 814 444 358 328 283 59
15
PfoI T'CCnGG_A
Cuts at: 1949 2693 3007 3999 4624 4928 1949
Size: 744 314 992 625 304 5321
Fragments arranged by size:
5321 992 744 625 314 304
PmeI GTTT'AAAC
Cuts at: 5299 5299
Size: 8300
PmlI CAC'GTG
Cuts at: 3210 3210
Size: 8300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6746 6778 6746
Size: 32 8268
PpuMI rG'GwC_Cy
Cuts at: 316 364 630 707 867 3011 5181 316
Size: 48 266 77 160 2144 2170 3435
Fragments arranged by size:
3435 2170 2144 266 160 77 48
PshAI GACnn'nnGTC
Cuts at: 1905 2408 1905
Size: 503 7797
PspOMI G'GGCC_C
Cuts at: 242 3315 4859 242
Size: 3073 1544 3683
Fragments arranged by size:
3683 3073 1544
PstI C_TGCA'G
Cuts at: 2294 3997 4380 5347 6032 2294
Size: 1703 383 967 685 4562
Fragments arranged by size:
4562 1703 967 685 383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8300
PvuII CAG'CTG
Cuts at: 1804 2348 3248 3574 3600 3629 5107 5725 6085
Size: 544 900 326 26 29 1478 618 360
Cuts at: 6085 1804
Size: 4019
Fragments arranged by size:
4019 1478 900 618 544 360 326 29
26
RsrII CG'GwC_CG
Cuts at: 6495 6495
Size: 8300
SacI G_AGCT'C
Cuts at: 3058 4172 5314 3058
Size: 1114 1142 6044
Fragments arranged by size:
6044 1142 1114
SacII CC_GC'GG
Cuts at: 5156 5156
Size: 8300
SalI G'TCGA_C
Cuts at: 5337 5337
Size: 8300
SanDI GG'GwC_CC
Cuts at: 316 316
Size: 8300
SapI GCTCTTCn'nnn_
Cuts at: 803 2962 3746 3802 4692 6323 6533 803
Size: 2159 784 56 890 1631 210 2570
Fragments arranged by size:
2570 2159 1631 890 784 210 56
SbfI CC_TGCA'GG
Cuts at: 5347 5347
Size: 8300
ScaI AGT'ACT
Cuts at: 7817 7817
Size: 8300
SfcI C'TryA_G
Cuts at: 33 2290 2733 2988 3993 4376 4937 5343 6028
Size: 2257 443 255 1005 383 561 406 685
Cuts at: 6028 6996 7187 33
Size: 968 191 1146
Fragments arranged by size:
2257 1146 1005 968 685 561 443 406
383 255 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4544 4544
Size: 8300
SfoI GGC'GCC
Cuts at: 4154 5732 5980 4154
Size: 1578 248 6474
Fragments arranged by size:
6474 1578 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8300
SmaI CCC'GGG
Cuts at: 160 241 313 1169 2071 5322 160
Size: 81 72 856 902 3251 3138
Fragments arranged by size:
3251 3138 902 856 81 72
SmlI C'TyrA_G
Cuts at: 927 1813 2102 3705 5455 6370 7084 7346 927
Size: 886 289 1603 1750 915 714 262 1881
Fragments arranged by size:
1881 1750 1603 915 886 714 289 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8300
SphI G_CATG'C
Cuts at: 5353 5853 5353
Size: 500 7800
SrfI GCCC'GGGC
Cuts at: 2071 2071
Size: 8300
StuI AGG'CCT
Cuts at: 414 427 974 414
Size: 13 547 7740
Fragments arranged by size:
7740 547 13
StyI C'CwwG_G
Cuts at: 140 409 1343 1461 1892 2268 2379 2754 2850
Size: 269 934 118 431 376 111 375 96
Cuts at: 2850 3132 3960 4286 4361 4502 4539 4743 4886
Size: 282 828 326 75 141 37 204 143
Cuts at: 4886 5027 5434 5788 140
Size: 141 407 354 2652
Fragments arranged by size:
2652 934 828 431 407 376 375 354
326 282 269 204 143 141 141 118
111 96 75 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 463 1279 1428 1722 1780 2098 2202 2779 5883
Size: 816 149 294 58 318 104 577 3104
Cuts at: 5883 6016 6475 6684 463
Size: 133 459 209 2079
Fragments arranged by size:
3104 2079 816 577 459 318 294 209
149 133 104 58
TatI w'GTAC_w
Cuts at: 7815 7815
Size: 8300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4106 4138 4910 4942 5196 5228 7817 7849 4106
Size: 32 772 32 254 32 2589 32 4557
Fragments arranged by size:
4557 2589 772 254 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6097 6097
Size: 8300
XbaI T'CTAG_A
Cuts at: 5331 5331
Size: 8300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 376 1774 3214 4284 5795 376
Size: 1398 1440 1070 1511 2881
Fragments arranged by size:
2881 1511 1440 1398 1070
XmaI C'CCGG_G
Cuts at: 158 239 311 1167 2069 5320 158
Size: 81 72 856 902 3251 3138
Fragments arranged by size:
3251 3138 902 856 81 72
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AgeI AlfI AscI AvrII BaeI BfrBI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstZ17I ClaI
EcoRV FspAI HpaI MfeI NdeI NotI NsiI PacI
PsiI PspXI PsrI SexAI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI