Restriction map for
pF1KA1746
(Circular) (Six-base) MAPSORT of: pF1KA1746.seq Check: 9786 from: 1 to: 6395
pF1KA1746 6395 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 840 1087 3257 840
Size: 247 2170 3978
Fragments arranged by size:
3978 2170 247
AatII G_ACGT'C
Cuts at: 2831 2831
Size: 6395
AccI GT'mk_AC
Cuts at: 3433 3433
Size: 6395
Acc65I G'GTAC_C
Cuts at: 1422 3411 1422
Size: 1989 4406
AclI AA'CG_TT
Cuts at: 6235 6235
Size: 6395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 872 2281 3274 4220 4652 5399 5667 313
Size: 559 1409 993 946 432 747 268 1041
Fragments arranged by size:
1409 1041 993 946 747 559 432 268
AflIII A'CryG_T
Cuts at: 3168 3747 4851 5828 3168
Size: 579 1104 977 3735
Fragments arranged by size:
3735 1104 977 579
AgeI A'CCGG_T
Cuts at: 4783 4783
Size: 6395
AhdI GACnn_n'nnGTC
Cuts at: 152 1851 152
Size: 1699 4696
AleI CACnn'nnGTG
Cuts at: 970 2098 970
Size: 1128 5267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 804 2558 804
Size: 1754 4641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 920 952 2213 2245 2966 2998 920
Size: 32 1261 32 721 32 4317
Fragments arranged by size:
4317 1261 721 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1242 2184 3069 3363 3700 5267 1242
Size: 942 885 294 337 1567 2370
Fragments arranged by size:
2370 1567 942 885 337 294
ApaI G_GGCC'C
Cuts at: 999 999
Size: 6395
ApaLI G'TGCA_C
Cuts at: 5165 5165
Size: 6395
ApoI r'AATT_y
Cuts at: 628 2657 3399 5748 6004 628
Size: 2029 742 2349 256 1019
Fragments arranged by size:
2349 2029 1019 742 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6395
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6395
AvaI C'yCGr_G
Cuts at: 195 204 1840 3415 195
Size: 9 1636 1575 3175
Fragments arranged by size:
3175 1636 1575 9
AvrII C'CTAG_G
Cuts at: 475 475
Size: 6395
BamHI G'GATC_C
Cuts at: 297 3420 297
Size: 3123 3272
BanI G'GyrC_C
Cuts at: 634 1422 3411 3825 4073 4108 634
Size: 788 1989 414 248 35 2921
Fragments arranged by size:
2921 1989 788 414 248 35
BanII G_rGCy'C
Cuts at: 141 196 211 508 999 1958 2576 3088 3278
Size: 55 15 297 491 959 618 512 190
Cuts at: 3278 3409 4439 141
Size: 131 1030 2097
Fragments arranged by size:
2097 1030 959 618 512 491 297 190
131 55 15
BbeI G_GCGC'C
Cuts at: 3829 4077 3829
Size: 248 6147
BbsI GAAGACnn'nnnn_
Cuts at: 541 693 982 2783 541
Size: 152 289 1801 4153
Fragments arranged by size:
4153 1801 289 152
BbvCI CC'TCA_GC
Cuts at: 1127 1127
Size: 6395
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2301 4293 5054 6395 1821
Size: 480 1992 761 1341 1821
Fragments arranged by size:
1992 1821 1341 761 480
BclI T'GATC_A
Cuts at: 3915 3915
Size: 6395
BfrBI ATG'CAT
Cuts at: 1466 1466
Size: 6395
BglI GCCn_nnn'nGGC
Cuts at: 112 121 200 2155 2377 3307 112
Size: 9 79 1955 222 930 3200
Fragments arranged by size:
3200 1955 930 222 79 9
BglII A'GATC_T
Cuts at: 2418 3910 5947 2418
Size: 1492 2037 2866
Fragments arranged by size:
2866 2037 1492
BlpI GC'TnA_GC
Cuts at: 190 725 2185 3220 3507 190
Size: 535 1460 1035 287 3078
Fragments arranged by size:
3078 1460 1035 535 287
Bme1580I G_kGCm'C
Cuts at: 999 1700 4020 4113 5169 999
Size: 701 2320 93 1056 2225
Fragments arranged by size:
2320 2225 1056 701 93
BmgBI CAC'GTC
Cuts at: 651 3285 651
Size: 2634 3761
BmrI ACTGGGnnnn_n'
Cuts at: 805 951 3297 3607 3688 4023 6167 805
Size: 146 2346 310 81 335 2144 1033
Fragments arranged by size:
2346 2144 1033 335 310 146 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1604 1977 4762 1604
Size: 373 2785 3237
Fragments arranged by size:
3237 2785 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1342 1947 3292 3385 925
Size: 417 605 1345 93 3935
Fragments arranged by size:
3935 1345 605 417 93
Bpu10I CC'TnA_GC
Cuts at: 140 502 915 1127 140
Size: 362 413 212 5408
Fragments arranged by size:
5408 413 362 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 903 1721 2543 2731 2746 3217 3571 4450
Size: 708 818 822 188 15 471 354 879
Cuts at: 4450 4942 5240 195
Size: 492 298 1350
Fragments arranged by size:
1350 879 822 818 708 492 471 354
298 188 15
BsaI GGTCTCn'nnnn_
Cuts at: 1044 2893 2964 6099 1044
Size: 1849 71 3135 1340
Fragments arranged by size:
3135 1849 1340 71
BsaAI yAC'GTr
Cuts at: 1491 3261 3748 1491
Size: 1770 487 4138
Fragments arranged by size:
4138 1770 487
BsaBI GATnn'nnATC
Cuts at: 6 1756 3909 5946 6
Size: 1750 2153 2037 455
Fragments arranged by size:
2153 2037 1750 455
BsaHI Gr'CG_yC
Cuts at: 1014 2828 3826 4074 4776 6272 1014
Size: 1814 998 248 702 1496 1137
Fragments arranged by size:
1814 1496 1137 998 702 248
BsaWI w'CCGG_w
Cuts at: 483 2310 3584 4105 4783 5057 5204 5615 483
Size: 1827 1274 521 678 274 147 411 1263
Fragments arranged by size:
1827 1274 1263 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 271 301 2213 2243 2942 2972 271
Size: 30 1912 30 699 30 3694
Fragments arranged by size:
3694 1912 699 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 1364 2237 871
Size: 493 873 5029
Fragments arranged by size:
5029 873 493
BseYI C'CCAG_C
Cuts at: 999 1190 2575 3081 3820 4578 5155 5651 999
Size: 191 1385 506 739 758 577 496 1743
Fragments arranged by size:
1743 1385 758 739 577 506 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 948
Size: 6395
BsiEI CG_ry'CG
Cuts at: 104 3937 3983 5191 104
Size: 3833 46 1208 1308
Fragments arranged by size:
3833 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2167 3024 3409 4187 4377 5169 2167
Size: 857 385 778 190 792 3393
Fragments arranged by size:
3393 857 792 778 385 190
BsmI GAATG_Cn'
Cuts at: 1873 1873
Size: 6395
Bsp1286I G_dGCh'C
Cuts at: 141 196 211 508 999 1700 1958 2167 2576
Size: 55 15 297 491 701 258 209 409
Cuts at: 2576 3024 3088 3278 3409 4020 4113 4187 4377
Size: 448 64 190 131 611 93 74 190
Cuts at: 4377 4439 5169 141
Size: 62 730 1367
Fragments arranged by size:
1367 730 701 611 491 448 409 297
258 209 190 190 131 93 74 64
62 55 15
BspEI T'CCGG_A
Cuts at: 483 5615 483
Size: 5132 1263
BspHI T'CATG_A
Cuts at: 2457 3332 5571 2457
Size: 875 2239 3281
Fragments arranged by size:
3281 2239 875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 840 1087 3257 3445 3659 3961 4342 772
Size: 68 247 2170 188 214 302 381 2825
Fragments arranged by size:
2825 2170 381 302 247 214 188 68
BsrBI CCG'CTC
Cuts at: 50 2022 4687 4741 6225 6390 50
Size: 1972 2665 54 1484 165 55
Fragments arranged by size:
2665 1972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2122 4307 2122
Size: 2185 4210
BsrFI r'CCGG_y
Cuts at: 3387 4393 4574 4783 3387
Size: 1006 181 209 4999
Fragments arranged by size:
4999 1006 209 181
BsrGI T'GTAC_A
Cuts at: 692 692
Size: 6395
BssSI C'ACGA_G
Cuts at: 4666 5024 4666
Size: 358 6037
BstAPI GCAn_nnn'nTGC
Cuts at: 2271 3772 2271
Size: 1501 4894
BstBI TT'CG_AA
Cuts at: 4756 4756
Size: 6395
BstXI CCAn_nnnn'nTGG
Cuts at: 766 2202 3039 3171 766
Size: 1436 837 132 3990
Fragments arranged by size:
3990 1436 837 132
BstYI r'GATC_y
Cuts at: 1 297 2418 3009 3237 3420 3887 3910 4245
Size: 296 2121 591 228 183 467 23 335
Cuts at: 4245 4491 5503 5589 5601 5947 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2121 1012 591 467 449 346 335 296
246 228 183 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2995 2995
Size: 6395
BtgI C'CryG_G
Cuts at: 107 125 2168 2195 2358 3089 107
Size: 18 2043 27 163 731 3413
Fragments arranged by size:
3413 2043 731 163 27 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4345 4526 4689 4345
Size: 181 163 6051
Fragments arranged by size:
6051 181 163
BtsI GCAGTG_nn'
Cuts at: 1857 3638 1857
Size: 1781 4614
ClaI AT'CG_AT
Cuts at: 1383 1383
Size: 6395
DraI TTT'AAA
Cuts at: 257 3394 257
Size: 3137 3258
DraIII CAC_nnn'GTG
Cuts at: 3264 3264
Size: 6395
DrdI GACnn_nn'nnGTC
Cuts at: 4101 4959 4101
Size: 858 5537
EaeI y'GGCC_r
Cuts at: 355 3041 3125 3287 3980 4154 4545 4572 5917
Size: 2686 84 162 693 174 391 27 1345
Cuts at: 5917 355
Size: 833
Fragments arranged by size:
2686 1345 833 693 391 174 162 84
27
EagI C'GGCC_G
Cuts at: 3980 3980
Size: 6395
EarI CTCTTCn'nnn_
Cuts at: 833 848 1382 2006 2432 3311 4418 4628 833
Size: 15 534 624 426 879 1107 210 2600
Fragments arranged by size:
2600 1107 879 624 534 426 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 427 4603 4913 5059 6205 427
Size: 4176 310 146 1146 617
Fragments arranged by size:
4176 1146 617 310 146
EcoICRI GAG'CTC
Cuts at: 3407 3407
Size: 6395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 872 925 1342 1947 2281 3274 3292 3385
Size: 559 53 417 605 334 993 18 93
Cuts at: 3385 4220 4652 5399 5667 313
Size: 835 432 747 268 1041
Fragments arranged by size:
1041 993 835 747 605 559 432 417
334 268 93 53 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2994 2994
Size: 6395
EcoO109I rG'GnC_Cy
Cuts at: 708 878 996 3534 708
Size: 170 118 2538 3569
Fragments arranged by size:
3569 2538 170 118
EcoRI G'AATT_C
Cuts at: 3399 3399
Size: 6395
EcoRV GAT'ATC
Cuts at: 774 774
Size: 6395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1471 1992 2650 2758 2772 4414 1471
Size: 521 658 108 14 1642 3452
Fragments arranged by size:
3452 1642 658 521 108 14
FspI TGC'GCA
Cuts at: 4176 4176
Size: 6395
HaeII r_GCGC'y
Cuts at: 3227 3829 4077 5099 6078 3227
Size: 602 248 1022 979 3544
Fragments arranged by size:
3544 1022 979 602 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 975 1007 1016 1048 1837 1869 2053
Size: 32 805 32 9 32 789 32 184
Cuts at: 2053 2085 2275 2307 2942 2944 2974 2976 4237
Size: 32 190 32 635 2 30 2 1261
Cuts at: 4237 4267 4269 4299 138
Size: 30 2 30 2234
Fragments arranged by size:
2234 1261 805 789 635 190 184 32
32 32 32 32 32 30 30 30
9 2 2 2
HincII GTy'rAC
Cuts at: 1913 3434 1913
Size: 1521 4874
HindIII A'AGCT_T
Cuts at: 2740 3292 2740
Size: 552 5843
KasI G'GCGC_C
Cuts at: 3825 4073 3825
Size: 248 6147
KpnI G_GTAC'C
Cuts at: 1426 3415 1426
Size: 1989 4406
MfeI C'AATT_G
Cuts at: 1655 1655
Size: 6395
MluI A'CGCG_T
Cuts at: 5828 5828
Size: 6395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 582 1664 2261 2318 2686 5066 5250 582
Size: 1082 597 57 368 2380 184 1727
Fragments arranged by size:
2380 1727 1082 597 368 184 57
MscI TGG'CCA
Cuts at: 357 3043 3127 3289 4156 357
Size: 2686 84 162 867 2596
Fragments arranged by size:
2686 2596 867 162 84
MslI CAynn'nnrTG
Cuts at: 970 2098 2194 3169 4384 4511 970
Size: 1128 96 975 1215 127 2854
Fragments arranged by size:
2854 1215 1128 975 127 96
MspA1I CmG'CkG
Cuts at: 134 701 1029 1111 2043 3506 3820 4180 5193
Size: 567 328 82 932 1463 314 360 1013
Cuts at: 5193 5438 134
Size: 245 1091
Fragments arranged by size:
1463 1091 1013 932 567 360 328 314
245 82
NaeI GCC'GGC
Cuts at: 4576 4576
Size: 6395
NarI GG'CG_CC
Cuts at: 3826 4074 3826
Size: 248 6147
NcoI C'CATG_G
Cuts at: 107 2195 2358 107
Size: 2088 163 4144
Fragments arranged by size:
4144 2088 163
NgoMIV G'CCGG_C
Cuts at: 4574 4574
Size: 6395
NsiI A_TGCA'T
Cuts at: 1468 1468
Size: 6395
NspI r_CATG'y
Cuts at: 152 894 2193 3172 3448 3948 4855 152
Size: 742 1299 979 276 500 907 1692
Fragments arranged by size:
1692 1299 979 907 742 500 276
PciI A'CATG_T
Cuts at: 3168 4851 3168
Size: 1683 4712
PflMI CCAn_nnn'nTGG
Cuts at: 1242 3069 1242
Size: 1827 4568
PfoI T'CCnGG_A
Cuts at: 923 923
Size: 6395
PmeI GTTT'AAAC
Cuts at: 3394 3394
Size: 6395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2213 2245 5534 5566 2213
Size: 32 3289 32 3042
Fragments arranged by size:
3289 3042 32 32
PsiI TTA'TAA
Cuts at: 1312 1312
Size: 6395
PspOMI G'GGCC_C
Cuts at: 995 995
Size: 6395
PstI C_TGCA'G
Cuts at: 837 2042 2537 3442 4127 837
Size: 1205 495 905 685 3105
Fragments arranged by size:
3105 1205 905 685 495
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6395
PvuII CAG'CTG
Cuts at: 701 1029 1111 2043 3820 4180 701
Size: 328 82 932 1777 360 2916
Fragments arranged by size:
2916 1777 932 360 328 82
RsrII CG'GwC_CG
Cuts at: 4590 4590
Size: 6395
SacI G_AGCT'C
Cuts at: 3409 3409
Size: 6395
SalI G'TCGA_C
Cuts at: 3432 3432
Size: 6395
SapI GCTCTTCn'nnn_
Cuts at: 2432 3311 4418 4628 2432
Size: 879 1107 210 4199
Fragments arranged by size:
4199 1107 879 210
SbfI CC_TGCA'GG
Cuts at: 3442 3442
Size: 6395
ScaI AGT'ACT
Cuts at: 5912 5912
Size: 6395
SfcI C'TryA_G
Cuts at: 33 220 833 1218 2038 2533 3438 4123 5116
Size: 187 613 385 820 495 905 685 993
Cuts at: 5116 5307 33
Size: 191 1121
Fragments arranged by size:
1121 993 905 820 685 613 495 385
191 187
SfiI GGCCn_nnn'nGGCC
Cuts at: 121 121
Size: 6395
SfoI GGC'GCC
Cuts at: 3827 4075 3827
Size: 248 6147
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6395
SmaI CCC'GGG
Cuts at: 197 3417 197
Size: 3220 3175
SmlI C'TyrA_G
Cuts at: 210 882 1736 2558 2725 2746 3232 3550 4465
Size: 672 854 822 167 21 486 318 915
Cuts at: 4465 4957 5219 210
Size: 492 262 1386
Fragments arranged by size:
1386 915 854 822 672 492 486 318
262 167 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6395
SphI G_CATG'C
Cuts at: 3448 3948 3448
Size: 500 5895
StuI AGG'CCT
Cuts at: 2531 2531
Size: 6395
StyI C'CwwG_G
Cuts at: 107 475 569 2195 2358 3048 3529 3883 107
Size: 368 94 1626 163 690 481 354 2619
Fragments arranged by size:
2619 1626 690 481 368 354 163 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3978 4111 4570 4779 3978
Size: 133 459 209 5594
Fragments arranged by size:
5594 459 209 133
TatI w'GTAC_w
Cuts at: 692 2401 5910 692
Size: 1709 3509 1177
Fragments arranged by size:
3509 1709 1177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 289 321 1140 1172 5912 5944 289
Size: 32 819 32 4740 32 740
Fragments arranged by size:
4740 819 740 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2956 4192 2956
Size: 1236 5159
XbaI T'CTAG_A
Cuts at: 3426 3426
Size: 6395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2202 3890 2202
Size: 1688 4707
XmaI C'CCGG_G
Cuts at: 195 3415 195
Size: 3220 3175
XmnI GAAnn'nnTTC
Cuts at: 842 3340 842
Size: 2498 3897
ZraI GAC'GTC
Cuts at: 2829 2829
Size: 6395
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BcgI BmtI BsiWI BsmBI
BssHII BstEII BstZ17I CspCI FseI FspAI HpaI NdeI
NheI NotI NruI PacI PasI PmlI PpuMI PshAI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI