Restriction map for
pF1KA1757
(Circular) (Six-base) MAPSORT of: pF1KA1757.seq Check: 9198 from: 1 to: 7106
pF1KA1757 7106 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 2076 2076
Size: 7106
AccI GT'mk_AC
Cuts at: 223 1220 3971 4144 223
Size: 997 2751 173 3185
Fragments arranged by size:
3185 2751 997 173
Acc65I G'GTAC_C
Cuts at: 4122 4122
Size: 7106
AclI AA'CG_TT
Cuts at: 6946 6946
Size: 7106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1130 1475 1490 1775 3523 3543 3626 4931
Size: 756 345 15 285 1748 20 83 1305
Cuts at: 4931 5363 6110 6378 374
Size: 432 747 268 1102
Fragments arranged by size:
1748 1305 1102 756 747 432 345 285
268 83 20 15
AfeI AGC'GCT
Cuts at: 2122 2122
Size: 7106
AflIII A'CryG_T
Cuts at: 1985 4458 5562 6539 1985
Size: 2473 1104 977 2552
Fragments arranged by size:
2552 2473 1104 977
AgeI A'CCGG_T
Cuts at: 5494 5494
Size: 7106
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 7106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1700 1700
Size: 7106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1031 1063 1031
Size: 32 7074
AlwNI CAG_nnn'CTG
Cuts at: 1804 2111 3070 3409 3498 4062 4411 5978 1804
Size: 307 959 339 89 564 349 1567 2932
Fragments arranged by size:
2932 1567 959 564 349 339 307 89
ApaLI G'TGCA_C
Cuts at: 4039 5876 4039
Size: 1837 5269
ApoI r'AATT_y
Cuts at: 342 363 499 763 1628 3015 3160 4110 6459
Size: 21 136 264 865 1387 145 950 2349
Cuts at: 6459 6715 342
Size: 256 733
Fragments arranged by size:
2349 1387 950 865 733 264 256 145
136 21
AseI AT'TA_AT
Cuts at: 20 533 20
Size: 513 6593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7106
AvaI C'yCGr_G
Cuts at: 2756 4126 2756
Size: 1370 5736
BamHI G'GATC_C
Cuts at: 1831 1956 2893 3124 4131 1831
Size: 125 937 231 1007 4806
Fragments arranged by size:
4806 1007 937 231 125
BanI G'GyrC_C
Cuts at: 3106 4122 4536 4784 4819 3106
Size: 1016 414 248 35 5393
Fragments arranged by size:
5393 1016 414 248 35
BanII G_rGCy'C
Cuts at: 2263 3365 4120 5150 2263
Size: 1102 755 1030 4219
Fragments arranged by size:
4219 1102 1030 755
BbeI G_GCGC'C
Cuts at: 4540 4788 4540
Size: 248 6858
BbsI GAAGACnn'nnnn_
Cuts at: 1535 1535
Size: 7106
BbvCI CC'TCA_GC
Cuts at: 923 3129 923
Size: 2206 4900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3088 3122 3088
Size: 34 7072
BciVI GTATCCnnnnn_n'
Cuts at: 1209 2419 2530 2982 3580 5004 5765 7106 1209
Size: 1210 111 452 598 1424 761 1341 1209
Fragments arranged by size:
1424 1341 1210 1209 761 598 452 111
BclI T'GATC_A
Cuts at: 1206 4626 1206
Size: 3420 3686
BfrBI ATG'CAT
Cuts at: 1332 1332
Size: 7106
BglI GCCn_nnn'nGGC
Cuts at: 1608 1608
Size: 7106
BglII A'GATC_T
Cuts at: 4621 6658 4621
Size: 2037 5069
BlpI GC'TnA_GC
Cuts at: 3441 4218 3441
Size: 777 6329
Bme1580I G_kGCm'C
Cuts at: 1927 1939 3111 4043 4731 4824 5880 1927
Size: 12 1172 932 688 93 1056 3153
Fragments arranged by size:
3153 1172 1056 932 688 93 12
BmgBI CAC'GTC
Cuts at: 3665 3665
Size: 7106
BmrI ACTGGGnnnn_n'
Cuts at: 3944 4318 4399 4734 6878 3944
Size: 374 81 335 2144 4172
Fragments arranged by size:
4172 2144 374 335 81
BmtI G_CTAG'C
Cuts at: 3812 3812
Size: 7106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3417 5473 3417
Size: 2056 5050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2315 3001 3566 3966 4039 1577
Size: 738 686 565 400 73 4644
Fragments arranged by size:
4644 738 686 565 400 73
Bpu10I CC'TnA_GC
Cuts at: 923 3008 3129 923
Size: 2085 121 4900
Fragments arranged by size:
4900 2085 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3233 4282 5161 5653 5951 3233
Size: 1049 879 492 298 4388
Fragments arranged by size:
4388 1049 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3606 6810 3606
Size: 3204 3902
BsaAI yAC'GTr
Cuts at: 1945 4459 1945
Size: 2514 4592
BsaBI GATnn'nnATC
Cuts at: 6 2669 4620 6657 6
Size: 2663 1951 2037 455
Fragments arranged by size:
2663 2037 1951 455
BsaHI Gr'CG_yC
Cuts at: 2073 4537 4785 5487 6983 2073
Size: 2464 248 702 1496 2196
Fragments arranged by size:
2464 2196 1496 702 248
BsaWI w'CCGG_w
Cuts at: 620 998 4295 4816 5494 5768 5915 6326 620
Size: 378 3297 521 678 274 147 411 1400
Fragments arranged by size:
3297 1400 678 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1031 1061 2530 2560 3449 3479 3518 3548 3564
Size: 30 1469 30 889 30 39 30 16
Cuts at: 3564 3594 3964 3994 4020 4050 1031
Size: 30 370 30 26 30 4087
Fragments arranged by size:
4087 1469 889 370 39 30 30 30
30 30 30 30 26 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 1049 1165 2425 2474 2600 2774 3801 211
Size: 838 116 1260 49 126 174 1027 3516
Fragments arranged by size:
3516 1260 1027 838 174 126 116 49
BseYI C'CCAG_C
Cuts at: 188 389 591 2372 3070 3955 4531 5289 5866
Size: 201 202 1781 698 885 576 758 577
Cuts at: 5866 6362 188
Size: 496 932
Fragments arranged by size:
1781 932 885 758 698 577 576 496
202 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 473 3026 3139 3590 335
Size: 138 2553 113 451 3851
Fragments arranged by size:
3851 2553 451 138 113
BsiEI CG_ry'CG
Cuts at: 104 1618 4648 4694 5902 104
Size: 1514 3030 46 1208 1308
Fragments arranged by size:
3030 1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 752 4043 4120 4898 5088 5880 752
Size: 3291 77 778 190 792 1978
Fragments arranged by size:
3291 1978 792 778 190 77
BsmI GAATG_Cn'
Cuts at: 2751 2751
Size: 7106
BsmBI CGTCTCn'nnnn_
Cuts at: 2064 2064
Size: 7106
Bsp1286I G_dGCh'C
Cuts at: 752 1927 1939 2263 3111 3365 4043 4120 4731
Size: 1175 12 324 848 254 678 77 611
Cuts at: 4731 4824 4898 5088 5150 5880 752
Size: 93 74 190 62 730 1978
Fragments arranged by size:
1978 1175 848 730 678 611 324 254
190 93 77 74 62 12
BspEI T'CCGG_A
Cuts at: 6326 6326
Size: 7106
BspHI T'CATG_A
Cuts at: 1209 6282 1209
Size: 5073 2033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4156 4370 4672 5053 4156
Size: 214 302 381 6209
Fragments arranged by size:
6209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5398 5452 6936 7101 50
Size: 5348 54 1484 165 55
Fragments arranged by size:
5348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2214 3916 5018 2214
Size: 1702 1102 4302
Fragments arranged by size:
4302 1702 1102
BsrFI r'CCGG_y
Cuts at: 5104 5285 5494 5104
Size: 181 209 6716
Fragments arranged by size:
6716 209 181
BsrGI T'GTAC_A
Cuts at: 459 835 459
Size: 376 6730
BssSI C'ACGA_G
Cuts at: 605 2680 2888 5377 5735 605
Size: 2075 208 2489 358 1976
Fragments arranged by size:
2489 2075 1976 358 208
BstAPI GCAn_nnn'nTGC
Cuts at: 4483 4483
Size: 7106
BstBI TT'CG_AA
Cuts at: 5467 5467
Size: 7106
BstEII G'GTnAC_C
Cuts at: 927 927
Size: 7106
BstYI r'GATC_y
Cuts at: 1 626 1831 1956 2779 2893 3124 4131 4598
Size: 625 1205 125 823 114 231 1007 467
Cuts at: 4598 4621 4956 5202 6214 6300 6312 6658 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1205 1012 1007 823 625 467 449 346
335 246 231 125 114 86 23 12
BstZ17I GTA'TAC
Cuts at: 224 224
Size: 7106
Bsu36I CC'TnA_GG
Cuts at: 631 631
Size: 7106
BtgI C'CryG_G
Cuts at: 3111 4023 3111
Size: 912 6194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5056 5237 5400 5056
Size: 181 163 6762
Fragments arranged by size:
6762 181 163
BtsI GCAGTG_nn'
Cuts at: 180 3237 3508 4060 4349 180
Size: 3057 271 552 289 2937
Fragments arranged by size:
3057 2937 552 289 271
DraI TTT'AAA
Cuts at: 4105 4105
Size: 7106
DraIII CAC_nnn'GTG
Cuts at: 1519 2908 3690 4024 1519
Size: 1389 782 334 4601
Fragments arranged by size:
4601 1389 782 334
DrdI GACnn_nn'nnGTC
Cuts at: 1667 4812 5670 1667
Size: 3145 858 3103
Fragments arranged by size:
3145 3103 858
EaeI y'GGCC_r
Cuts at: 1615 2378 4691 4865 5256 5283 6628 1615
Size: 763 2313 174 391 27 1345 2093
Fragments arranged by size:
2313 2093 1345 763 391 174 27
EagI C'GGCC_G
Cuts at: 1615 4691 1615
Size: 3076 4030
EarI CTCTTCn'nnn_
Cuts at: 591 757 1241 1250 1436 1615 1715 2229 2402
Size: 166 484 9 186 179 100 514 173
Cuts at: 2402 3663 5129 5339 591
Size: 1261 1466 210 2358
Fragments arranged by size:
2358 1466 1261 514 484 210 186 179
173 166 100 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 156 5314 5624 5770 6916 156
Size: 5158 310 146 1146 346
Fragments arranged by size:
5158 1146 346 310 146
EcoICRI GAG'CTC
Cuts at: 4118 4118
Size: 7106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1130 1475 1490 1577 1775 2315 3001 3523
Size: 756 345 15 87 198 540 686 522
Cuts at: 3523 3543 3566 3626 3966 4039 4931 5363 6110
Size: 20 23 60 340 73 892 432 747
Cuts at: 6110 6378 374
Size: 268 1102
Fragments arranged by size:
1102 892 756 747 686 540 522 432
345 340 268 198 87 73 60 23
20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 2840
Size: 7106
EcoO109I rG'GnC_Cy
Cuts at: 1610 2081 2918 3324 3826 4245 1610
Size: 471 837 406 502 419 4471
Fragments arranged by size:
4471 837 502 471 419 406
EcoRI G'AATT_C
Cuts at: 342 4110 342
Size: 3768 3338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5125 5125
Size: 7106
FspI TGC'GCA
Cuts at: 4887 4887
Size: 7106
HaeII r_GCGC'y
Cuts at: 2124 4540 4788 5810 6789 2124
Size: 2416 248 1022 979 2441
Fragments arranged by size:
2441 2416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1153 1185 1823 1855 2172 2204 2417
Size: 32 979 32 638 32 317 32 213
Cuts at: 2417 2449 3299 3331 3927 3959 4948 4978 4980
Size: 32 850 32 596 32 989 30 2
Cuts at: 4980 5010 142
Size: 30 2238
Fragments arranged by size:
2238 989 979 850 638 596 317 213
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1842 4145 1842
Size: 2303 4803
HindIII A'AGCT_T
Cuts at: 431 431
Size: 7106
KasI G'GCGC_C
Cuts at: 4536 4784 4536
Size: 248 6858
KpnI G_GTAC'C
Cuts at: 4126 4126
Size: 7106
MluI A'CGCG_T
Cuts at: 6539 6539
Size: 7106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1984 2573 3122 5777 5961 1984
Size: 589 549 2655 184 3129
Fragments arranged by size:
3129 2655 589 549 184
MscI TGG'CCA
Cuts at: 2380 4867 2380
Size: 2487 4619
MslI CAynn'nnrTG
Cuts at: 2816 5095 5222 2816
Size: 2279 127 4700
Fragments arranged by size:
4700 2279 127
MspA1I CmG'CkG
Cuts at: 591 2376 2561 3967 4217 4531 4891 5904 6149
Size: 1785 185 1406 250 314 360 1013 245
Cuts at: 6149 591
Size: 1548
Fragments arranged by size:
1785 1548 1406 1013 360 314 250 245
185
NaeI GCC'GGC
Cuts at: 5287 5287
Size: 7106
NarI GG'CG_CC
Cuts at: 4537 4785 4537
Size: 248 6858
NcoI C'CATG_G
Cuts at: 3111 3111
Size: 7106
NgoMIV G'CCGG_C
Cuts at: 5285 5285
Size: 7106
NheI G'CTAG_C
Cuts at: 3808 3808
Size: 7106
NsiI A_TGCA'T
Cuts at: 1334 1334
Size: 7106
NspI r_CATG'y
Cuts at: 1336 1989 4159 4659 5566 1336
Size: 653 2170 500 907 2876
Fragments arranged by size:
2876 2170 907 653 500
PciI A'CATG_T
Cuts at: 1985 5562 1985
Size: 3577 3529
PflMI CCAn_nnn'nTGG
Cuts at: 3754 4024 3754
Size: 270 6836
PfoI T'CCnGG_A
Cuts at: 3582 3582
Size: 7106
PmeI GTTT'AAAC
Cuts at: 4105 4105
Size: 7106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1031 1063 6245 6277 1031
Size: 32 5182 32 1860
Fragments arranged by size:
5182 1860 32 32
PpuMI rG'GwC_Cy
Cuts at: 3324 3826 3324
Size: 502 6604
PsiI TTA'TAA
Cuts at: 667 968 667
Size: 301 6805
PspXI vC'TCGA_Gb
Cuts at: 2756 2756
Size: 7106
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 467 499 467
Size: 32 7074
PstI C_TGCA'G
Cuts at: 1569 2841 3066 3244 3414 3503 3824 4153 4838
Size: 1272 225 178 170 89 321 329 685
Cuts at: 4838 1569
Size: 3837
Fragments arranged by size:
3837 1272 685 329 321 225 178 170
89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7106
PvuII CAG'CTG
Cuts at: 591 2376 2561 3967 4531 4891 591
Size: 1785 185 1406 564 360 2806
Fragments arranged by size:
2806 1785 1406 564 360 185
RsrII CG'GwC_CG
Cuts at: 5301 5301
Size: 7106
SacI G_AGCT'C
Cuts at: 4120 4120
Size: 7106
SalI G'TCGA_C
Cuts at: 4143 4143
Size: 7106
SanDI GG'GwC_CC
Cuts at: 3826 3826
Size: 7106
SapI GCTCTTCn'nnn_
Cuts at: 757 2229 5129 5339 757
Size: 1472 2900 210 2524
Fragments arranged by size:
2900 2524 1472 210
SbfI CC_TGCA'GG
Cuts at: 3824 4153 3824
Size: 329 6777
ScaI AGT'ACT
Cuts at: 6623 6623
Size: 7106
SexAI A'CCwGG_T
Cuts at: 1992 2904 1992
Size: 912 6194
SfcI C'TryA_G
Cuts at: 33 1034 1565 2488 2837 3062 3240 3410 3499
Size: 1001 531 923 349 225 178 170 89
Cuts at: 3499 3820 4149 4834 5827 6018 33
Size: 321 329 685 993 191 1121
Fragments arranged by size:
1121 1001 993 923 685 531 349 329
321 225 191 178 170 89
SfoI GGC'GCC
Cuts at: 4538 4786 4538
Size: 248 6858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7106
SmaI CCC'GGG
Cuts at: 4128 4128
Size: 7106
SmlI C'TyrA_G
Cuts at: 2756 3248 4261 5176 5668 5930 2756
Size: 492 1013 915 492 262 3932
Fragments arranged by size:
3932 1013 915 492 492 262
SnaBI TAC'GTA
Cuts at: 1945 1945
Size: 7106
SpeI A'CTAG_T
Cuts at: 67 1575 67
Size: 1508 5598
SphI G_CATG'C
Cuts at: 1336 4159 4659 1336
Size: 2823 500 3783
Fragments arranged by size:
3783 2823 500
StuI AGG'CCT
Cuts at: 3979 3979
Size: 7106
StyI C'CwwG_G
Cuts at: 914 1379 1715 3111 3320 3781 4240 4594 914
Size: 465 336 1396 209 461 459 354 3426
Fragments arranged by size:
3426 1396 465 461 459 354 336 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2414 4689 4822 5281 5490 2414
Size: 2275 133 459 209 4030
Fragments arranged by size:
4030 2275 459 209 133
TatI w'GTAC_w
Cuts at: 446 459 835 1965 6621 446
Size: 13 376 1130 4656 931
Fragments arranged by size:
4656 1130 931 376 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1941 1973 2045 2077 3518 3550 3676 3708 3879
Size: 32 72 32 1441 32 126 32 171
Cuts at: 3879 3911 4020 4052 6623 6655 1941
Size: 32 109 32 2571 32 2392
Fragments arranged by size:
2571 2392 1441 171 126 109 72 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4903 4903
Size: 7106
XbaI T'CTAG_A
Cuts at: 1767 4137 1767
Size: 2370 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2270 3702 3751 4601 2270
Size: 1432 49 850 4775
Fragments arranged by size:
4775 1432 850 49
XhoI C'TCGA_G
Cuts at: 2756 2756
Size: 7106
XmaI C'CCGG_G
Cuts at: 4126 4126
Size: 7106
ZraI GAC'GTC
Cuts at: 2074 2074
Size: 7106
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AleI ApaI AscI AvrII BaeI BsiWI
BssHII BstXI ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI PacI PasI PmlI PshAI
PspOMI SacII SfiI SgrAI SrfI SspI SwaI XmnI