Restriction map for
pF1KA1760
(Circular) (Six-base) MAPSORT of: pF1KA1760.seq Check: 7147 from: 1 to: 9743
pF1KA1760 9743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2348 3919 5040 6384 2348
Size: 1571 1121 1344 5707
Fragments arranged by size:
5707 1571 1344 1121
AatII G_ACGT'C
Cuts at: 1202 1640 2837 3170 4363 5384 1202
Size: 438 1197 333 1193 1021 5561
Fragments arranged by size:
5561 1197 1193 1021 438 333
AccI GT'mk_AC
Cuts at: 692 5384 6781 692
Size: 4692 1397 3654
Fragments arranged by size:
4692 3654 1397
Acc65I G'GTAC_C
Cuts at: 6421 6759 6421
Size: 338 9405
AclI AA'CG_TT
Cuts at: 3799 9583 3799
Size: 5784 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 946 1003 1543 2410 5800 5947 6310 6511
Size: 12 57 540 867 3390 147 363 201
Cuts at: 6511 6532 7568 8000 8353 9015 934
Size: 21 1036 432 353 662 1662
Fragments arranged by size:
3390 1662 1036 867 662 540 432 363
353 201 147 57 21 12
AfeI AGC'GCT
Cuts at: 184 365 184
Size: 181 9562
AflIII A'CryG_T
Cuts at: 3589 3644 5118 7095 8895 9176 3589
Size: 55 1474 1977 1800 281 4156
Fragments arranged by size:
4156 1977 1800 1474 281 55
AhdI GACnn_n'nnGTC
Cuts at: 4122 4122
Size: 9743
AleI CACnn'nnGTG
Cuts at: 1438 1631 1438
Size: 193 9550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2230 2444 5869 2230
Size: 214 3425 6104
Fragments arranged by size:
6104 3425 214
AlwNI CAG_nnn'CTG
Cuts at: 276 460 981 2061 2295 3135 3911 4167 4303
Size: 184 521 1080 234 840 776 256 136
Cuts at: 4303 4341 4450 5763 6355 6528 7048 8486 276
Size: 38 109 1313 592 173 520 1438 1533
Fragments arranged by size:
1533 1438 1313 1080 840 776 592 521
520 256 234 184 173 136 109 38
ApaI G_GGCC'C
Cuts at: 134 179 259 2045 2227 2379 3371 4183 4628
Size: 45 80 1786 182 152 992 812 445
Cuts at: 4628 5506 5629 134
Size: 878 123 4248
Fragments arranged by size:
4248 1786 992 878 812 445 182 152
123 80 45
ApaLI G'TGCA_C
Cuts at: 1327 8581 1327
Size: 7254 2489
ApoI r'AATT_y
Cuts at: 6747 9096 9352 6747
Size: 2349 256 7138
Fragments arranged by size:
7138 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9743
AvaI C'yCGr_G
Cuts at: 115 152 412 559 1147 1162 1816 1831 3370
Size: 37 260 147 588 15 654 15 1539
Cuts at: 3370 4036 4354 4958 5234 5507 5609 5629 5728
Size: 666 318 604 276 273 102 20 99
Cuts at: 5728 6593 6763 115
Size: 865 170 3095
Fragments arranged by size:
3095 1539 865 666 654 604 588 318
276 273 260 170 147 102 99 37
20 15 15
AvrII C'CTAG_G
Cuts at: 6303 6303
Size: 9743
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2782 2815 2782
Size: 33 9710
BamHI G'GATC_C
Cuts at: 2166 6768 2166
Size: 4602 5141
BanI G'GyrC_C
Cuts at: 249 968 1158 1770 2412 2664 2823 3529 3922
Size: 719 190 612 642 252 159 706 393
Cuts at: 3922 4200 5883 6421 6759 7173 7421 7456 249
Size: 278 1683 538 338 414 248 35 2536
Fragments arranged by size:
2536 1683 719 706 642 612 538 414
393 338 278 252 248 190 159 35
BanII G_rGCy'C
Cuts at: 116 134 179 259 387 396 419 437 560
Size: 18 45 80 128 9 23 18 123
Cuts at: 560 584 1454 1832 1930 2045 2119 2227 2379
Size: 24 870 378 98 115 74 108 152
Cuts at: 2379 3077 3260 3371 4183 4355 4487 4511 4628
Size: 698 183 111 812 172 132 24 117
Cuts at: 4628 4826 5129 5174 5477 5506 5629 5729 6053
Size: 198 303 45 303 29 123 100 324
Cuts at: 6053 6757 7787 116
Size: 704 1030 2072
Fragments arranged by size:
2072 1030 870 812 704 698 378 324
303 303 198 183 172 152 132 128
123 123 117 115 111 108 100 98
80 74 45 45 29 24 24 23
18 18 9
BbeI G_GCGC'C
Cuts at: 253 1162 2416 2668 2827 3533 7177 7425 253
Size: 909 1254 252 159 706 3644 248 2571
Fragments arranged by size:
3644 2571 1254 909 706 252 248 159
BbsI GAAGACnn'nnnn_
Cuts at: 922 1514 6119 922
Size: 592 4605 4546
Fragments arranged by size:
4605 4546 592
BbvCI CC'TCA_GC
Cuts at: 3904 4770 4776 3904
Size: 866 6 8871
Fragments arranged by size:
8871 866 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2265 2299 3730 3764 2265
Size: 34 1431 34 8244
Fragments arranged by size:
8244 1431 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7641 8697 9743 7641
Size: 1056 1046 7641
Fragments arranged by size:
7641 1056 1046
BclI T'GATC_A
Cuts at: 7263 7263
Size: 9743
BglI GCCn_nnn'nGGC
Cuts at: 3366 4039 4178 4216 4312 5843 3366
Size: 673 139 38 96 1531 7266
Fragments arranged by size:
7266 1531 673 139 96 38
BglII A'GATC_T
Cuts at: 1277 5786 7258 9295 1277
Size: 4509 1472 2037 1725
Fragments arranged by size:
4509 2037 1725 1472
BlpI GC'TnA_GC
Cuts at: 1408 2384 3847 4022 6147 6855 1408
Size: 976 1463 175 2125 708 4296
Fragments arranged by size:
4296 2125 1463 976 708 175
Bme1580I G_kGCm'C
Cuts at: 134 179 259 427 1331 1775 2045 2227 2379
Size: 45 80 168 904 444 270 182 152
Cuts at: 2379 2471 3317 3371 3925 4183 4203 4476 4628
Size: 92 846 54 554 258 20 273 152
Cuts at: 4628 4763 4984 5194 5506 5629 6686 7368 7461
Size: 135 221 210 312 123 1057 682 93
Cuts at: 7461 8585 134
Size: 1124 1292
Fragments arranged by size:
1292 1124 1057 904 846 682 554 444
312 273 270 258 221 210 182 168
152 152 135 123 93 92 80 54
45 20
BmgBI CAC'GTC
Cuts at: 4985 5415 6384 6616 4985
Size: 430 969 232 8112
Fragments arranged by size:
8112 969 430 232
BmrI ACTGGGnnnn_n'
Cuts at: 1079 2004 3665 4876 5685 6955 7036 7371 9515
Size: 925 1661 1211 809 1270 81 335 2144
Cuts at: 9515 1079
Size: 1307
Fragments arranged by size:
2144 1661 1307 1270 1211 925 809 335
81
BmtI G_CTAG'C
Cuts at: 5213 5213
Size: 9743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3450 4470 4917 5418 5624 8110 3450
Size: 1020 447 501 206 2486 5083
Fragments arranged by size:
5083 2486 1020 501 447 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1246 1591 2134 2305 2342 2383 2555 3064
Size: 459 345 543 171 37 41 172 509
Cuts at: 3064 3092 3571 3703 4250 4421 4427 4678 4840
Size: 28 479 132 547 171 6 251 162
Cuts at: 4840 5174 5388 5964 5988 787
Size: 334 214 576 24 4542
Fragments arranged by size:
4542 576 547 543 509 479 459 345
334 251 214 172 171 171 162 132
41 37 28 24 6
Bpu10I CC'TnA_GC
Cuts at: 554 3071 3176 3254 3904 4168 4505 4770 4776
Size: 2517 105 78 650 264 337 265 6
Cuts at: 4776 6023 6073 6356 554
Size: 1247 50 283 3941
Fragments arranged by size:
3941 2517 1247 650 337 283 265 264
105 78 50 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 634 2417 5950 6919 7798 8512 8810 598
Size: 36 1783 3533 969 879 714 298 1531
Fragments arranged by size:
3533 1783 1531 969 879 714 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 706 1025 4905 5103 5508 9447 706
Size: 319 3880 198 405 3939 1002
Fragments arranged by size:
3939 3880 1002 405 319 198
BsaAI yAC'GTr
Cuts at: 3755 4758 5119 7096 3755
Size: 1003 361 1977 6402
Fragments arranged by size:
6402 1977 1003 361
BsaBI GATnn'nnATC
Cuts at: 6 7257 9294 6
Size: 7251 2037 455
Fragments arranged by size:
7251 2037 455
BsaHI Gr'CG_yC
Cuts at: 250 339 404 1012 1159 1199 1582 1637 2413
Size: 89 65 608 147 40 383 55 776
Cuts at: 2413 2665 2824 2834 3167 3530 3863 4360 4466
Size: 252 159 10 333 363 333 497 106
Cuts at: 4466 5381 5536 7174 7422 8124 9620 250
Size: 915 155 1638 248 702 1496 373
Fragments arranged by size:
1638 1496 915 776 702 608 497 383
373 363 333 333 252 248 159 155
147 106 89 65 55 40 10
BsaWI w'CCGG_w
Cuts at: 749 1060 4014 6932 7453 8131 8542 8689 8963
Size: 311 2954 2918 521 678 411 147 274
Cuts at: 8963 749
Size: 1529
Fragments arranged by size:
2954 2918 1529 678 521 411 311 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 2526 2556 3859 3889 4425 4455 6124
Size: 30 1124 30 1303 30 536 30 1669
Cuts at: 6124 6154 1372
Size: 30 4961
Fragments arranged by size:
4961 1669 1303 1124 536 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 514 604 607 1189 1474 1849 2249 3439
Size: 279 90 3 582 285 375 400 1190
Cuts at: 3439 3946 4215 4758 5439 235
Size: 507 269 543 681 4539
Fragments arranged by size:
4539 1190 681 582 543 507 400 375
285 279 269 90 3
BseYI C'CCAG_C
Cuts at: 179 666 1807 2070 2203 2270 2619 2777 2802
Size: 487 1141 263 133 67 349 158 25
Cuts at: 2802 3147 3274 4141 4183 4206 4510 4601 4780
Size: 345 127 867 42 23 304 91 179
Cuts at: 4780 4922 5077 5622 5638 6095 6368 6554 6624
Size: 142 155 545 16 457 273 186 70
Cuts at: 6624 7168 7926 8591 8999 179
Size: 544 758 665 408 923
Fragments arranged by size:
1141 923 867 758 665 545 544 487
457 408 349 345 304 273 263 186
179 158 155 142 133 127 91 70
67 42 25 23 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 2040 2081 2881 3325 3967 4423 4484 5099
Size: 1055 41 800 444 642 456 61 615
Cuts at: 5099 5545 6109 6364 6424 985
Size: 446 564 255 60 4304
Fragments arranged by size:
4304 1055 800 642 615 564 456 446
444 255 61 60 41
BsiEI CG_ry'CG
Cuts at: 104 172 515 542 642 2531 3870 7285 7331
Size: 68 343 27 100 1889 1339 3415 46
Cuts at: 7331 8561 104
Size: 1230 1286
Fragments arranged by size:
3415 1889 1339 1286 1230 343 100 68
46 27
BsiHKAI G_wGCw'C
Cuts at: 1181 1331 1454 3260 4028 4355 4757 5729 6246
Size: 150 123 1806 768 327 402 972 517
Cuts at: 6246 6757 7535 7725 8585 1181
Size: 511 778 190 860 2339
Fragments arranged by size:
2339 1806 972 860 778 768 517 511
402 327 190 150 123
BsmI GAATG_Cn'
Cuts at: 1272 1272
Size: 9743
BsmBI CGTCTCn'nnnn_
Cuts at: 1573 4351 4367 5015 6301 6720 1573
Size: 2778 16 648 1286 419 4596
Fragments arranged by size:
4596 2778 1286 648 419 16
Bsp1286I G_dGCh'C
Cuts at: 116 134 179 259 387 396 419 427 437
Size: 18 45 80 128 9 23 8 10
Cuts at: 437 560 584 1181 1331 1454 1775 1832 1930
Size: 123 24 597 150 123 321 57 98
Cuts at: 1930 2045 2119 2227 2379 2471 3077 3260 3317
Size: 115 74 108 152 92 606 183 57
Cuts at: 3317 3371 3925 4028 4183 4203 4355 4476 4487
Size: 54 554 103 155 20 152 121 11
Cuts at: 4487 4511 4628 4757 4763 4826 4984 5129 5174
Size: 24 117 129 6 63 158 145 45
Cuts at: 5174 5194 5477 5506 5629 5729 6053 6246 6686
Size: 20 283 29 123 100 324 193 440
Cuts at: 6686 6757 7368 7461 7535 7725 7787 8585 116
Size: 71 611 93 74 190 62 798 1274
Fragments arranged by size:
1274 798 611 606 597 554 440 324
321 283 193 190 183 158 155 152
152 150 145 129 128 123 123 123
121 117 115 108 103 100 98 93
92 80 74 74 71 63 62 57
57 54 45 45 29 24 24 23
20 20 18 11 10 9 8 6
BspHI T'CATG_A
Cuts at: 8175 8175
Size: 9743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1412 1770 2280 2348 2531 3572 3919 5040 5106
Size: 358 510 68 183 1041 347 1121 66
Cuts at: 5106 5121 6384 6446 6793 7007 7309 7690 1412
Size: 15 1263 62 347 214 302 381 3465
Fragments arranged by size:
3465 1263 1121 1041 510 381 358 347
347 302 214 183 68 66 62 15
BsrBI CCG'CTC
Cuts at: 50 771 2383 3175 3792 4847 6077 8035 8089
Size: 721 1612 792 617 1055 1230 1958 54
Cuts at: 8089 9573 9738 50
Size: 1484 165 55
Fragments arranged by size:
1958 1612 1484 1230 1055 792 721 617
165 55 54
BsrDI GCAATG_nn'
Cuts at: 7655 7655
Size: 9743
BsrFI r'CCGG_y
Cuts at: 406 971 1308 1767 2109 2325 2706 2889 3051
Size: 565 337 459 342 216 381 183 162
Cuts at: 3051 3201 3620 5614 7741 7922 406
Size: 150 419 1994 2127 181 2227
Fragments arranged by size:
2227 2127 1994 565 459 419 381 342
337 216 183 181 162 150
BssHII G'CGCG_C
Cuts at: 327 3595 327
Size: 3268 6475
BssSI C'ACGA_G
Cuts at: 1627 4384 4411 6239 8014 8722 1627
Size: 2757 27 1828 1775 708 2648
Fragments arranged by size:
2757 2648 1828 1775 708 27
BstAPI GCAn_nnn'nTGC
Cuts at: 276 872 968 1860 2061 3018 3312 5763 7120
Size: 596 96 892 201 957 294 2451 1357
Cuts at: 7120 276
Size: 2899
Fragments arranged by size:
2899 2451 1357 957 892 596 294 201
96
BstBI TT'CG_AA
Cuts at: 8104 8104
Size: 9743
BstEII G'GTnAC_C
Cuts at: 1290 1788 3705 1290
Size: 498 1917 7328
Fragments arranged by size:
7328 1917 498
BstXI CCAn_nnnn'nTGG
Cuts at: 633 1893 2659 3282 3759 5298 5646 6342 633
Size: 1260 766 623 477 1539 348 696 4034
Fragments arranged by size:
4034 1539 1260 766 696 623 477 348
BstYI r'GATC_y
Cuts at: 1 977 1277 1700 2166 5786 6768 7235 7258
Size: 976 300 423 466 3620 982 467 23
Cuts at: 7258 7593 7839 8145 8157 8243 9295 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
3620 1052 982 976 467 466 449 423
335 306 300 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 903 1492 4334 4889 903
Size: 589 2842 555 5757
Fragments arranged by size:
5757 2842 589 555
BtgI C'CryG_G
Cuts at: 107 134 398 419 620 672 1193 1467 2090
Size: 27 264 21 201 52 521 274 623
Cuts at: 2090 2219 2309 2771 3287 3870 6647 107
Size: 129 90 462 516 583 2777 3203
Fragments arranged by size:
3203 2777 623 583 521 516 462 274
264 201 129 90 52 27 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 303 435 1470 7693 7874 8037 303
Size: 132 1035 6223 181 163 2009
Fragments arranged by size:
6223 2009 1035 181 163 132
BtsI GCAGTG_nn'
Cuts at: 2893 2911 3055 4580 5432 5851 6053 6092 6986
Size: 18 144 1525 852 419 202 39 894
Cuts at: 6986 2893
Size: 5650
Fragments arranged by size:
5650 1525 894 852 419 202 144 39
18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1853 1888 1853
Size: 35 9708
DraI TTT'AAA
Cuts at: 6742 6742
Size: 9743
DraIII CAC_nnn'GTG
Cuts at: 2859 2859
Size: 9743
DrdI GACnn_nn'nnGTC
Cuts at: 5004 6222 7449 8793 5004
Size: 1218 1227 1344 5954
Fragments arranged by size:
5954 1344 1227 1218
EaeI y'GGCC_r
Cuts at: 169 539 623 639 1100 1232 1310 1649 1920
Size: 370 84 16 461 132 78 339 271
Cuts at: 1920 2171 2247 2672 2708 2726 3141 3284 4592
Size: 251 76 425 36 18 415 143 1308
Cuts at: 4592 5895 6174 7328 7502 7893 7920 9265 169
Size: 1303 279 1154 174 391 27 1345 647
Fragments arranged by size:
1345 1308 1303 1154 647 461 425 415
391 370 339 279 271 251 174 143
132 84 78 76 36 27 18 16
EagI C'GGCC_G
Cuts at: 169 539 639 7328 169
Size: 370 100 6689 2584
Fragments arranged by size:
6689 2584 370 100
EarI CTCTTCn'nnn_
Cuts at: 581 782 3988 7766 7976 581
Size: 201 3206 3778 210 2348
Fragments arranged by size:
3778 3206 2348 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 1754 6179 7951 8693 8839 9553 160
Size: 1594 4425 1772 742 146 714 350
Fragments arranged by size:
4425 1772 1594 742 714 350 146
EcoICRI GAG'CTC
Cuts at: 1452 3258 4353 5727 6755 1452
Size: 1806 1095 1374 1028 4440
Fragments arranged by size:
4440 1806 1374 1095 1028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 934 946 1003 1246 1543 1591 2134 2305
Size: 147 12 57 243 297 48 543 171
Cuts at: 2305 2342 2383 2410 2555 3064 3092 3571 3703
Size: 37 41 27 145 509 28 479 132
Cuts at: 3703 4250 4421 4427 4678 4840 5174 5388 5800
Size: 547 171 6 251 162 334 214 412
Cuts at: 5800 5947 5964 5988 6310 6511 6532 7568 8000
Size: 147 17 24 322 201 21 1036 432
Cuts at: 8000 8353 9015 787
Size: 353 662 1515
Fragments arranged by size:
1515 1036 662 547 543 509 479 432
412 353 334 322 297 251 243 214
201 171 171 162 147 147 145 132
57 48 41 37 28 27 24 21
17 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1573 2287 4171 4458 5392 6359 6596 1573
Size: 714 1884 287 934 967 237 4720
Fragments arranged by size:
4720 1884 967 934 714 287 237
EcoO109I rG'GnC_Cy
Cuts at: 130 175 176 987 2041 2075 2292 2375 2376
Size: 45 1 811 1054 34 217 83 1
Cuts at: 2376 3180 4041 4180 4389 4526 4624 4784 4866
Size: 804 861 139 209 137 98 160 82
Cuts at: 4866 5246 5397 5502 5503 5626 5658 6013 6027
Size: 380 151 105 1 123 32 355 14
Cuts at: 6027 6326 6352 6364 6882 130
Size: 299 26 12 518 2991
Fragments arranged by size:
2991 1054 861 811 804 518 380 355
299 217 209 160 151 139 137 123
105 98 83 82 45 34 32 26
14 12 1 1 1
EcoRI G'AATT_C
Cuts at: 6747 6747
Size: 9743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3738 6441 7762 3738
Size: 2703 1321 5719
Fragments arranged by size:
5719 2703 1321
FspI TGC'GCA
Cuts at: 7524 7524
Size: 9743
HaeII r_GCGC'y
Cuts at: 186 253 285 367 860 1162 1886 1999 2416
Size: 67 32 82 493 302 724 113 417
Cuts at: 2416 2668 2827 3533 4108 5234 6631 7177 7425
Size: 252 159 706 575 1126 1397 546 248
Cuts at: 7425 8655 9426 186
Size: 1230 771 503
Fragments arranged by size:
1397 1230 1126 771 724 706 575 546
503 493 417 302 252 248 159 113
82 67 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1128 1160 2526 2558 3859 3891 4108 4140 4279
Size: 32 1366 32 1301 32 217 32 139
Cuts at: 4279 4311 5266 5298 5464 5496 5811 5843 6124
Size: 32 955 32 166 32 315 32 281
Cuts at: 6124 6156 6576 6600 6608 6632 7585 7615 7617
Size: 32 420 24 8 24 953 30 2
Cuts at: 7617 7647 1128
Size: 30 3224
Fragments arranged by size:
3224 1366 1301 955 953 420 315 281
217 166 139 32 32 32 32 32
32 32 32 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 6782 6782
Size: 9743
KasI G'GCGC_C
Cuts at: 249 1158 2412 2664 2823 3529 7173 7421 249
Size: 909 1254 252 159 706 3644 248 2571
Fragments arranged by size:
3644 2571 1254 909 706 252 248 159
KpnI G_GTAC'C
Cuts at: 6425 6763 6425
Size: 338 9405
MluI A'CGCG_T
Cuts at: 3589 9176 3589
Size: 5587 4156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 3902 4643 8502 8686 191
Size: 3711 741 3859 184 1248
Fragments arranged by size:
3859 3711 1248 741 184
MscI TGG'CCA
Cuts at: 625 1102 1234 1651 2674 3143 3286 4594 6176
Size: 477 132 417 1023 469 143 1308 1582
Cuts at: 6176 7504 625
Size: 1328 2864
Fragments arranged by size:
2864 1582 1328 1308 1023 477 469 417
143 132
MslI CAynn'nnrTG
Cuts at: 1184 1438 1631 2212 3757 7732 7859 1184
Size: 254 193 581 1545 3975 127 3068
Fragments arranged by size:
3975 3068 1545 581 254 193 127
MspA1I CmG'CkG
Cuts at: 136 400 460 674 777 965 1919 2265 2388
Size: 264 60 214 103 188 954 346 123
Cuts at: 2388 2806 3112 3357 3402 3521 3681 4122 4523
Size: 418 306 245 45 119 160 441 401
Cuts at: 4523 4780 4917 6489 6510 6854 7168 7528 8312
Size: 257 137 1572 21 344 314 360 784
Cuts at: 8312 8557 136
Size: 245 1322
Fragments arranged by size:
1572 1322 954 784 441 418 401 360
346 344 314 306 264 257 245 245
214 188 160 137 123 119 103 60
45 21
NaeI GCC'GGC
Cuts at: 408 973 1310 2891 3053 3622 5616 7924 408
Size: 565 337 1581 162 569 1994 2308 2227
Fragments arranged by size:
2308 2227 1994 1581 569 565 337 162
NarI GG'CG_CC
Cuts at: 250 1159 2413 2665 2824 3530 7174 7422 250
Size: 909 1254 252 159 706 3644 248 2571
Fragments arranged by size:
3644 2571 1254 909 706 252 248 159
NcoI C'CATG_G
Cuts at: 107 107
Size: 9743
NgoMIV G'CCGG_C
Cuts at: 406 971 1308 2889 3051 3620 5614 7922 406
Size: 565 337 1581 162 569 1994 2308 2227
Fragments arranged by size:
2308 2227 1994 1581 569 565 337 162
NheI G'CTAG_C
Cuts at: 5209 5209
Size: 9743
NspI r_CATG'y
Cuts at: 1224 3846 6796 7296 8899 1224
Size: 2622 2950 500 1603 2068
Fragments arranged by size:
2950 2622 2068 1603 500
PasI CC'CwG_GG
Cuts at: 494 2659 3883 4620 4685 494
Size: 2165 1224 737 65 5552
Fragments arranged by size:
5552 2165 1224 737 65
PciI A'CATG_T
Cuts at: 8895 8895
Size: 9743
PflMI CCAn_nnn'nTGG
Cuts at: 3702 4929 3702
Size: 1227 8516
PfoI T'CCnGG_A
Cuts at: 2162 6657 2162
Size: 4495 5248
PmeI GTTT'AAAC
Cuts at: 6742 6742
Size: 9743
PmlI CAC'GTG
Cuts at: 4758 5119 4758
Size: 361 9382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8189 8221 8189
Size: 32 9711
PpuMI rG'GwC_Cy
Cuts at: 2292 3180 4526 4784 4866 5246 5658 6027 6326
Size: 888 1346 258 82 380 412 369 299
Cuts at: 6326 2292
Size: 5709
Fragments arranged by size:
5709 1346 888 412 380 369 299 258
82
PspOMI G'GGCC_C
Cuts at: 130 175 255 2041 2223 2375 3367 4179 4624
Size: 45 80 1786 182 152 992 812 445
Cuts at: 4624 5502 5625 130
Size: 878 123 4248
Fragments arranged by size:
4248 1786 992 878 812 445 182 152
123 80 45
PspXI vC'TCGA_Gb
Cuts at: 4958 5234 5728 6593 4958
Size: 276 494 865 8108
Fragments arranged by size:
8108 865 494 276
PstI C_TGCA'G
Cuts at: 281 746 815 1718 1781 2423 2636 3785 5600
Size: 465 69 903 63 642 213 1149 1815
Cuts at: 5600 5759 5801 6060 6790 7475 281
Size: 159 42 259 730 685 2549
Fragments arranged by size:
2549 1815 1149 903 730 685 642 465
259 213 159 69 63 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9743
PvuII CAG'CTG
Cuts at: 965 2388 3112 3402 3521 3681 4122 4780 4917
Size: 1423 724 290 119 160 441 658 137
Cuts at: 4917 6489 6510 7168 7528 965
Size: 1572 21 658 360 3180
Fragments arranged by size:
3180 1572 1423 724 658 658 441 360
290 160 137 119 21
RsrII CG'GwC_CG
Cuts at: 7938 7938
Size: 9743
SacI G_AGCT'C
Cuts at: 1454 3260 4355 5729 6757 1454
Size: 1806 1095 1374 1028 4440
Fragments arranged by size:
4440 1806 1374 1095 1028
SacII CC_GC'GG
Cuts at: 137 401 675 137
Size: 264 274 9205
Fragments arranged by size:
9205 274 264
SalI G'TCGA_C
Cuts at: 6780 6780
Size: 9743
SanDI GG'GwC_CC
Cuts at: 4526 4784 5246 4526
Size: 258 462 9023
Fragments arranged by size:
9023 462 258
SapI GCTCTTCn'nnn_
Cuts at: 7766 7976 7766
Size: 210 9533
SbfI CC_TGCA'GG
Cuts at: 1781 3785 6790 1781
Size: 2004 3005 4734
Fragments arranged by size:
4734 3005 2004
ScaI AGT'ACT
Cuts at: 9260 9260
Size: 9743
SexAI A'CCwGG_T
Cuts at: 6690 6690
Size: 9743
SfcI C'TryA_G
Cuts at: 33 277 742 811 1714 1777 2419 2632 3781
Size: 244 465 69 903 63 642 213 1149
Cuts at: 3781 5596 5755 5797 6056 6786 7471 8439 8630
Size: 1815 159 42 259 730 685 968 191
Cuts at: 8630 33
Size: 1146
Fragments arranged by size:
1815 1149 1146 968 903 730 685 642
465 259 244 213 191 159 69 63
42
SfoI GGC'GCC
Cuts at: 251 1160 2414 2666 2825 3531 7175 7423 251
Size: 909 1254 252 159 706 3644 248 2571
Fragments arranged by size:
3644 2571 1254 909 706 252 248 159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9743
SgrAI Cr'CCGG_yG
Cuts at: 1767 1767
Size: 9743
SmaI CCC'GGG
Cuts at: 117 561 1818 3372 6765 117
Size: 444 1257 1554 3393 3095
Fragments arranged by size:
3393 3095 1554 1257 444
SmlI C'TyrA_G
Cuts at: 613 649 2432 4354 4958 5234 5728 5965 6593
Size: 36 1783 1922 604 276 494 237 628
Cuts at: 6593 6898 7813 8527 8789 613
Size: 305 915 714 262 1567
Fragments arranged by size:
1922 1783 1567 915 714 628 604 494
305 276 262 237 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9743
SphI G_CATG'C
Cuts at: 1224 6796 7296 1224
Size: 5572 500 3671
Fragments arranged by size:
5572 3671 500
SrfI GCCC'GGGC
Cuts at: 117 561 1818 117
Size: 444 1257 8042
Fragments arranged by size:
8042 1257 444
SspI AAT'ATT
Cuts at: 1050 1050
Size: 9743
StuI AGG'CCT
Cuts at: 809 3433 5604 809
Size: 2624 2171 4948
Fragments arranged by size:
4948 2624 2171
StyI C'CwwG_G
Cuts at: 107 860 950 1535 1721 4477 4541 4928 6177
Size: 753 90 585 186 2756 64 387 1249
Cuts at: 6177 6303 6877 7231 107
Size: 126 574 354 2619
Fragments arranged by size:
2756 2619 1249 753 585 574 387 354
186 126 90 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 708 1032 1491 3161 3334 5652 6212 6738 7326
Size: 324 459 1670 173 2318 560 526 588
Cuts at: 7326 7459 7918 8127 708
Size: 133 459 209 2324
Fragments arranged by size:
2324 2318 1670 588 560 526 459 459
324 209 173 133
TatI w'GTAC_w
Cuts at: 1937 9258 1937
Size: 7321 2422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1007 1039 3568 3600 4425 4457 5036 5068 6639
Size: 32 2529 32 825 32 579 32 1571
Cuts at: 6639 6671 9260 9292 1007
Size: 32 2589 32 1458
Fragments arranged by size:
2589 2529 1571 1458 825 579 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6675 7540 6675
Size: 865 8878
XbaI T'CTAG_A
Cuts at: 6774 6774
Size: 9743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1804 2481 3136 3759 4716 6339 7238 1804
Size: 677 655 623 957 1623 899 4309
Fragments arranged by size:
4309 1623 957 899 677 655 623
XhoI C'TCGA_G
Cuts at: 4354 4958 5234 5728 6593 4354
Size: 604 276 494 865 7504
Fragments arranged by size:
7504 865 604 494 276
XmaI C'CCGG_G
Cuts at: 115 559 1816 3370 6763 115
Size: 444 1257 1554 3393 3095
Fragments arranged by size:
3393 3095 1554 1257 444
XmnI GAAnn'nnTTC
Cuts at: 931 931
Size: 9743
ZraI GAC'GTC
Cuts at: 1200 1638 2835 3168 4361 5382 1200
Size: 438 1197 333 1193 1021 5561
Fragments arranged by size:
5561 1197 1193 1021 438 333
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI BfrBI BsiWI BspEI BsrGI
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PshAI PsiI PsrI
SfiI SnaBI SwaI