Restriction map for
pF1KA1761
(Circular) (Six-base) MAPSORT of: pF1KA1761.seq Check: 2001 from: 1 to: 5624
pF1KA1761 5624 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2059 2059
Size: 5624
AatII G_ACGT'C
Cuts at: 1751 1751
Size: 5624
AccI GT'mk_AC
Cuts at: 1889 2662 1889
Size: 773 4851
Acc65I G'GTAC_C
Cuts at: 2640 2640
Size: 5624
AclI AA'CG_TT
Cuts at: 5464 5464
Size: 5624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 834 1112 1702 2269 3449 3881 4234 4896 834
Size: 278 590 567 1180 432 353 662 1562
Fragments arranged by size:
1562 1180 662 590 567 432 353 278
AflIII A'CryG_T
Cuts at: 1532 2976 4776 5057 1532
Size: 1444 1800 281 2099
Fragments arranged by size:
2099 1800 1444 281
AleI CACnn'nnGTG
Cuts at: 1474 1774 1474
Size: 300 5324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1637 1637
Size: 5624
AlwNI CAG_nnn'CTG
Cuts at: 1123 1917 2929 4367 1123
Size: 794 1012 1438 2380
Fragments arranged by size:
2380 1438 1012 794
ApaI G_GGCC'C
Cuts at: 136 2211 2466 136
Size: 2075 255 3294
Fragments arranged by size:
3294 2075 255
ApaLI G'TGCA_C
Cuts at: 993 4462 993
Size: 3469 2155
ApoI r'AATT_y
Cuts at: 385 922 1435 2628 4977 5233 385
Size: 537 513 1193 2349 256 776
Fragments arranged by size:
2349 1193 776 537 513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5624
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
AvaI C'yCGr_G
Cuts at: 205 542 1241 2644 205
Size: 337 699 1403 3185
Fragments arranged by size:
3185 1403 699 337
BamHI G'GATC_C
Cuts at: 425 1218 2649 425
Size: 793 1431 3400
Fragments arranged by size:
3400 1431 793
BanI G'GyrC_C
Cuts at: 210 2640 3054 3302 3337 210
Size: 2430 414 248 35 2497
Fragments arranged by size:
2497 2430 414 248 35
BanII G_rGCy'C
Cuts at: 136 353 868 1373 1383 2211 2466 2638 3668
Size: 217 515 505 10 828 255 172 1030
Cuts at: 3668 136
Size: 2092
Fragments arranged by size:
2092 1030 828 515 505 255 217 172
10
BbeI G_GCGC'C
Cuts at: 3058 3306 3058
Size: 248 5376
BbsI GAAGACnn'nnnn_
Cuts at: 988 2314 2574 988
Size: 1326 260 4038
Fragments arranged by size:
4038 1326 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 335 369 335
Size: 34 5590
BciVI GTATCCnnnnn_n'
Cuts at: 453 3522 4578 5624 453
Size: 3069 1056 1046 453
Fragments arranged by size:
3069 1056 1046 453
BclI T'GATC_A
Cuts at: 3144 3144
Size: 5624
BglII A'GATC_T
Cuts at: 3139 5176 3139
Size: 2037 3587
BlpI GC'TnA_GC
Cuts at: 2736 2736
Size: 5624
Bme1580I G_kGCm'C
Cuts at: 136 213 649 997 2211 2418 2466 3249 3342
Size: 77 436 348 1214 207 48 783 93
Cuts at: 3342 4466 136
Size: 1124 1294
Fragments arranged by size:
1294 1214 1124 783 436 348 207 93
77 48
BmgBI CAC'GTC
Cuts at: 1535 1535
Size: 5624
BmrI ACTGGGnnnn_n'
Cuts at: 2367 2836 2917 3252 5396 2367
Size: 469 81 335 2144 2595
Fragments arranged by size:
2595 2144 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 568 603 882 2601 3991 568
Size: 35 279 1719 1390 2201
Fragments arranged by size:
2201 1719 1390 279 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 658 1300 2112 331
Size: 327 642 812 3843
Fragments arranged by size:
3843 812 642 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 548 1438 1849 2242 2800 3679 4393 4691
Size: 31 890 411 393 558 879 714 298
Cuts at: 4691 517
Size: 1450
Fragments arranged by size:
1450 890 879 714 558 411 393 298
31
BsaI GGTCTCn'nnnn_
Cuts at: 784 5328 784
Size: 4544 1080
BsaAI yAC'GTr
Cuts at: 2977 2977
Size: 5624
BsaBI GATnn'nnATC
Cuts at: 6 3138 5175 6
Size: 3132 2037 455
Fragments arranged by size:
3132 2037 455
BsaHI Gr'CG_yC
Cuts at: 1748 3055 3303 4005 5501 1748
Size: 1307 248 702 1496 1871
Fragments arranged by size:
1871 1496 1307 702 248
BsaWI w'CCGG_w
Cuts at: 422 473 2813 3334 4012 4423 4570 4844 422
Size: 51 2340 521 678 411 147 274 1202
Fragments arranged by size:
2340 1202 678 521 411 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 1564 1594 304
Size: 30 1230 30 4334
Fragments arranged by size:
4334 1230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 622 1027 1270 1579 1774 2422 427
Size: 195 405 243 309 195 648 3629
Fragments arranged by size:
3629 648 405 309 243 195 195
BseYI C'CCAG_C
Cuts at: 161 861 1651 1794 1910 3049 3807 4472 4880
Size: 700 790 143 116 1139 758 665 408
Cuts at: 4880 161
Size: 905
Fragments arranged by size:
1139 905 790 758 700 665 408 143
116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 622 603
Size: 19 5605
BsiEI CG_ry'CG
Cuts at: 104 3166 3212 4442 104
Size: 3062 46 1230 1286
Fragments arranged by size:
3062 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 353 997 1172 2638 3416 3606 4466 353
Size: 644 175 1466 778 190 860 1511
Fragments arranged by size:
1511 1466 860 778 644 190 175
BsmI GAATG_Cn'
Cuts at: 1934 2154 1934
Size: 220 5404
BsmBI CGTCTCn'nnnn_
Cuts at: 1282 1282
Size: 5624
Bsp1286I G_dGCh'C
Cuts at: 136 213 353 649 868 997 1172 1373 1383
Size: 77 140 296 219 129 175 201 10
Cuts at: 1383 2211 2418 2466 2638 3249 3342 3416 3606
Size: 828 207 48 172 611 93 74 190
Cuts at: 3606 3668 4466 136
Size: 62 798 1294
Fragments arranged by size:
1294 828 798 611 296 219 207 201
190 175 172 140 129 93 77 74
62 48 10
BspEI T'CCGG_A
Cuts at: 422 422
Size: 5624
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 5624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2059 2607 2674 2888 3190 3571 2059
Size: 548 67 214 302 381 4112
Fragments arranged by size:
4112 548 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1215 1580 3916 3970 5454 5619 50
Size: 1165 365 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 1165 365 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2376 3536 2376
Size: 1160 4464
BsrFI r'CCGG_y
Cuts at: 114 183 3622 3803 114
Size: 69 3439 181 1935
Fragments arranged by size:
3439 1935 181 69
BsrGI T'GTAC_A
Cuts at: 875 875
Size: 5624
BssSI C'ACGA_G
Cuts at: 3895 4603 3895
Size: 708 4916
BstAPI GCAn_nnn'nTGC
Cuts at: 2037 3001 2037
Size: 964 4660
BstBI TT'CG_AA
Cuts at: 3985 3985
Size: 5624
BstEII G'GTnAC_C
Cuts at: 915 915
Size: 5624
BstXI CCAn_nnnn'nTGG
Cuts at: 735 2277 735
Size: 1542 4082
BstYI r'GATC_y
Cuts at: 1 425 1218 1685 2126 2649 3116 3139 3474
Size: 424 793 467 441 523 467 23 335
Cuts at: 3474 3720 4026 4038 4124 5176 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 793 523 467 467 449 441 424
335 306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 754 1070 1757 2537 754
Size: 316 687 780 3841
Fragments arranged by size:
3841 780 687 316
BtgI C'CryG_G
Cuts at: 107 214 224 1375 107
Size: 107 10 1151 4356
Fragments arranged by size:
4356 1151 107 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3574 3755 3918 3574
Size: 181 163 5280
Fragments arranged by size:
5280 181 163
BtsI GCAGTG_nn'
Cuts at: 512 714 936 1464 1982 2019 2293 2867 512
Size: 202 222 528 518 37 274 574 3269
Fragments arranged by size:
3269 574 528 518 274 222 202 37
DraI TTT'AAA
Cuts at: 2623 2623
Size: 5624
DraIII CAC_nnn'GTG
Cuts at: 1920 2025 1920
Size: 105 5519
DrdI GACnn_nn'nnGTC
Cuts at: 757 3330 4674 757
Size: 2573 1344 1707
Fragments arranged by size:
2573 1707 1344
EaeI y'GGCC_r
Cuts at: 3209 3383 3774 3801 5146 3209
Size: 174 391 27 1345 3687
Fragments arranged by size:
3687 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3209 3209
Size: 5624
EarI CTCTTCn'nnn_
Cuts at: 520 1241 1247 2600 3647 3857 520
Size: 721 6 1353 1047 210 2287
Fragments arranged by size:
2287 1353 1047 721 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 167 2068 3832 4574 4720 5434 167
Size: 1901 1764 742 146 714 357
Fragments arranged by size:
1901 1764 742 714 357 146
EcoICRI GAG'CTC
Cuts at: 351 2636 351
Size: 2285 3339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 658 834 1112 1300 1702 2112 2269 3449
Size: 327 176 278 188 402 410 157 1180
Cuts at: 3449 3881 4234 4896 331
Size: 432 353 662 1059
Fragments arranged by size:
1180 1059 662 432 410 402 353 327
278 188 176 157
EcoNI CCTnn'n_nnAGG
Cuts at: 637 1589 2536 637
Size: 952 947 3725
Fragments arranged by size:
3725 952 947
EcoO109I rG'GnC_Cy
Cuts at: 759 2208 2763 759
Size: 1449 555 3620
Fragments arranged by size:
3620 1449 555
EcoRI G'AATT_C
Cuts at: 385 2628 385
Size: 2243 3381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 5624
FspI TGC'GCA
Cuts at: 3405 3405
Size: 5624
HaeII r_GCGC'y
Cuts at: 2454 3058 3306 4536 5307 2454
Size: 604 248 1230 771 2771
Fragments arranged by size:
2771 1230 771 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 589 621 783 815 3466 3496 3498 3528 589
Size: 32 162 32 2651 30 2 30 2685
Fragments arranged by size:
2685 2651 162 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2663 2663
Size: 5624
KasI G'GCGC_C
Cuts at: 3054 3302 3054
Size: 248 5376
KpnI G_GTAC'C
Cuts at: 2644 2644
Size: 5624
MluI A'CGCG_T
Cuts at: 5057 5057
Size: 5624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4383 4567 4383
Size: 184 5440
MscI TGG'CCA
Cuts at: 3385 3385
Size: 5624
MslI CAynn'nnrTG
Cuts at: 1084 1408 1474 1774 3613 3740 1084
Size: 324 66 300 1839 127 2968
Fragments arranged by size:
2968 1839 324 300 127 66
MspA1I CmG'CkG
Cuts at: 216 226 873 2735 3049 3409 4193 4438 216
Size: 10 647 1862 314 360 784 245 1402
Fragments arranged by size:
1862 1402 784 647 360 314 245 10
NaeI GCC'GGC
Cuts at: 116 185 3805 116
Size: 69 3620 1935
Fragments arranged by size:
3620 1935 69
NarI GG'CG_CC
Cuts at: 3055 3303 3055
Size: 248 5376
NcoI C'CATG_G
Cuts at: 107 107
Size: 5624
NgoMIV G'CCGG_C
Cuts at: 114 183 3803 114
Size: 69 3620 1935
Fragments arranged by size:
3620 1935 69
NspI r_CATG'y
Cuts at: 2677 3177 4780 2677
Size: 500 1603 3521
Fragments arranged by size:
3521 1603 500
PciI A'CATG_T
Cuts at: 4776 4776
Size: 5624
PflMI CCAn_nnn'nTGG
Cuts at: 1917 2526 2600 1917
Size: 609 74 4941
Fragments arranged by size:
4941 609 74
PfoI T'CCnGG_A
Cuts at: 494 607 635 2006 494
Size: 113 28 1371 4112
Fragments arranged by size:
4112 1371 113 28
PmeI GTTT'AAAC
Cuts at: 2623 2623
Size: 5624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 5592
PpuMI rG'GwC_Cy
Cuts at: 759 759
Size: 5624
PshAI GACnn'nnGTC
Cuts at: 1274 1274
Size: 5624
PspOMI G'GGCC_C
Cuts at: 132 2207 2462 132
Size: 2075 255 3294
Fragments arranged by size:
3294 2075 255
PspXI vC'TCGA_Gb
Cuts at: 542 542
Size: 5624
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1873 1905 1873
Size: 32 5592
PstI C_TGCA'G
Cuts at: 348 706 1022 1626 1701 2507 2618 2671 3356
Size: 358 316 604 75 806 111 53 685
Cuts at: 3356 348
Size: 2616
Fragments arranged by size:
2616 806 685 604 358 316 111 75
53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5624
PvuII CAG'CTG
Cuts at: 3049 3409 3049
Size: 360 5264
RsrII CG'GwC_CG
Cuts at: 127 3819 127
Size: 3692 1932
SacI G_AGCT'C
Cuts at: 353 2638 353
Size: 2285 3339
SacII CC_GC'GG
Cuts at: 217 227 217
Size: 10 5614
SalI G'TCGA_C
Cuts at: 2661 2661
Size: 5624
SapI GCTCTTCn'nnn_
Cuts at: 2600 3647 3857 2600
Size: 1047 210 4367
Fragments arranged by size:
4367 1047 210
SbfI CC_TGCA'GG
Cuts at: 2671 2671
Size: 5624
ScaI AGT'ACT
Cuts at: 5141 5141
Size: 5624
SexAI A'CCwGG_T
Cuts at: 1916 1916
Size: 5624
SfcI C'TryA_G
Cuts at: 33 344 702 1018 1330 1622 1697 2503 2614
Size: 311 358 316 312 292 75 806 111
Cuts at: 2614 2667 3352 4320 4511 33
Size: 53 685 968 191 1146
Fragments arranged by size:
1146 968 806 685 358 316 312 311
292 191 111 75 53
SfoI GGC'GCC
Cuts at: 3056 3304 3056
Size: 248 5376
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
SmaI CCC'GGG
Cuts at: 207 2646 207
Size: 2439 3185
SmlI C'TyrA_G
Cuts at: 532 542 563 1241 1417 1864 2257 2779 3694
Size: 10 21 678 176 447 393 522 915
Cuts at: 3694 4408 4670 532
Size: 714 262 1486
Fragments arranged by size:
1486 915 714 678 522 447 393 262
176 21 10
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5624
SphI G_CATG'C
Cuts at: 2677 3177 2677
Size: 500 5124
StyI C'CwwG_G
Cuts at: 107 1385 1638 2389 2758 3112 107
Size: 1278 253 751 369 354 2619
Fragments arranged by size:
2619 1278 751 369 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1352 1719 3207 3340 3799 4008 1352
Size: 367 1488 133 459 209 2968
Fragments arranged by size:
2968 1488 459 367 209 133
TatI w'GTAC_w
Cuts at: 875 1529 5139 875
Size: 654 3610 1360
Fragments arranged by size:
3610 1360 654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5141 5173 5141
Size: 32 5592
Tth111I GACn'n_nGTC
Cuts at: 2313 3421 2313
Size: 1108 4516
XbaI T'CTAG_A
Cuts at: 2655 2655
Size: 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 3119 492
Size: 2627 2997
XhoI C'TCGA_G
Cuts at: 542 1241 542
Size: 699 4925
XmaI C'CCGG_G
Cuts at: 205 2644 205
Size: 2439 3185
ZraI GAC'GTC
Cuts at: 1749 1749
Size: 5624
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI Bpu10I BsiWI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI