Restriction map for
pF1KA1762
(Circular) (Six-base) MAPSORT of: pF1KA1762.seq Check: 431 from: 1 to: 7679
pF1KA1762 7679 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2856 3579 3591 4505 2856
Size: 723 12 914 6030
Fragments arranged by size:
6030 914 723 12
AccI GT'mk_AC
Cuts at: 4717 4717
Size: 7679
Acc65I G'GTAC_C
Cuts at: 4695 4695
Size: 7679
AclI AA'CG_TT
Cuts at: 7519 7519
Size: 7679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1673 2249 3208 3523 5504 5936 6289 6951
Size: 1284 576 959 315 1981 432 353 662
Cuts at: 6951 389
Size: 1117
Fragments arranged by size:
1981 1284 1117 959 662 576 432 353
315
AflII C'TTAA_G
Cuts at: 1024 1024
Size: 7679
AflIII A'CryG_T
Cuts at: 1458 5031 6831 7112 1458
Size: 3573 1800 281 2025
Fragments arranged by size:
3573 2025 1800 281
AleI CACnn'nnGTG
Cuts at: 3754 3754
Size: 7679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2369 2369
Size: 7679
AlwNI CAG_nnn'CTG
Cuts at: 349 1044 1627 2149 3346 3468 3541 3993 4984
Size: 695 583 522 1197 122 73 452 991
Cuts at: 4984 6422 349
Size: 1438 1606
Fragments arranged by size:
1606 1438 1197 991 695 583 522 452
122 73
ApaI G_GGCC'C
Cuts at: 889 1085 1606 2158 2688 2888 2986 4546 889
Size: 196 521 552 530 200 98 1560 4022
Fragments arranged by size:
4022 1560 552 530 521 200 196 98
ApaLI G'TGCA_C
Cuts at: 534 705 6517 534
Size: 171 5812 1696
Fragments arranged by size:
5812 1696 171
ApoI r'AATT_y
Cuts at: 4683 7032 7288 4683
Size: 2349 256 5074
Fragments arranged by size:
5074 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7679
AvaI C'yCGr_G
Cuts at: 785 1378 2687 3598 4699 785
Size: 593 1309 911 1101 3765
Fragments arranged by size:
3765 1309 1101 911 593
BamHI G'GATC_C
Cuts at: 4704 4704
Size: 7679
BanI G'GyrC_C
Cuts at: 661 1008 1728 2727 3627 3832 4425 4695 5109
Size: 347 720 999 900 205 593 270 414
Cuts at: 5109 5357 5392 661
Size: 248 35 2948
Fragments arranged by size:
2948 999 900 720 593 414 347 270
248 205 35
BanII G_rGCy'C
Cuts at: 428 739 842 889 1085 1606 2158 2519 2645
Size: 311 103 47 196 521 552 361 126
Cuts at: 2645 2688 2888 2986 3498 3869 4187 4396 4405
Size: 43 200 98 512 371 318 209 9
Cuts at: 4405 4546 4693 5723 428
Size: 141 147 1030 2384
Fragments arranged by size:
2384 1030 552 521 512 371 361 318
311 209 200 196 147 141 126 103
98 47 43 9
BbeI G_GCGC'C
Cuts at: 3631 5113 5361 3631
Size: 1482 248 5949
Fragments arranged by size:
5949 1482 248
BbsI GAAGACnn'nnnn_
Cuts at: 377 3964 377
Size: 3587 4092
BbvCI CC'TCA_GC
Cuts at: 398 398
Size: 7679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 794 2598 2632 760
Size: 34 1804 34 5807
Fragments arranged by size:
5807 1804 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5577 6633 7679 5577
Size: 1056 1046 5577
Fragments arranged by size:
5577 1056 1046
BclI T'GATC_A
Cuts at: 5199 5199
Size: 7679
BglI GCCn_nnn'nGGC
Cuts at: 1405 3722 1405
Size: 2317 5362
BglII A'GATC_T
Cuts at: 2373 3042 5194 7231 2373
Size: 669 2152 2037 2821
Fragments arranged by size:
2821 2152 2037 669
BlpI GC'TnA_GC
Cuts at: 1166 1175 2188 3659 4791 1166
Size: 9 1013 1471 1132 4054
Fragments arranged by size:
4054 1471 1132 1013 9
Bme1580I G_kGCm'C
Cuts at: 538 666 709 874 889 1013 1085 1346 1606
Size: 128 43 165 15 124 72 261 260
Cuts at: 1606 1650 2158 2688 2888 2986 4430 4546 5304
Size: 44 508 530 200 98 1444 116 758
Cuts at: 5304 5397 6521 538
Size: 93 1124 1696
Fragments arranged by size:
1696 1444 1124 758 530 508 261 260
200 165 128 124 116 98 93 72
44 43 15
BmrI ACTGGGnnnn_n'
Cuts at: 428 1964 2345 2828 2963 3137 3365 4891 4972
Size: 1536 381 483 135 174 228 1526 81
Cuts at: 4972 5307 7451 428
Size: 335 2144 656
Fragments arranged by size:
2144 1536 1526 656 483 381 335 228
174 135 81
BmtI G_CTAG'C
Cuts at: 1865 1865
Size: 7679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 447 3466 6046 447
Size: 3019 2580 2080
Fragments arranged by size:
3019 2580 2080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 326 431 766 1015 1689 1734 2123 2156
Size: 12 105 335 249 674 45 389 33
Cuts at: 2156 2277 2993 3116 3137 3870 4144 4211 314
Size: 121 716 123 21 733 274 67 3782
Fragments arranged by size:
3782 733 716 674 389 335 274 249
123 121 105 67 45 33 21 12
Bpu10I CC'TnA_GC
Cuts at: 263 398 1492 2555 4181 263
Size: 135 1094 1063 1626 3761
Fragments arranged by size:
3761 1626 1094 1063 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1426 2008 3463 3544 3922 4520 4855 5734
Size: 369 582 1455 81 378 598 335 879
Cuts at: 5734 6448 6746 1057
Size: 714 298 1990
Fragments arranged by size:
1990 1455 879 714 598 582 378 369
335 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 200 1580 3789 3888 7383 200
Size: 1380 2209 99 3495 496
Fragments arranged by size:
3495 2209 1380 496 99
BsaAI yAC'GTr
Cuts at: 5032 5032
Size: 7679
BsaBI GATnn'nnATC
Cuts at: 6 5193 7230 6
Size: 5187 2037 455
Fragments arranged by size:
5187 2037 455
BsaHI Gr'CG_yC
Cuts at: 3628 5110 5358 6060 7556 3628
Size: 1482 248 702 1496 3751
Fragments arranged by size:
3751 1496 1482 702 248
BsaWI w'CCGG_w
Cuts at: 1942 4868 5389 6067 6478 6625 6899 1942
Size: 2926 521 678 411 147 274 2722
Fragments arranged by size:
2926 2722 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 721 751 1125 1155 2611 2641 3868 3898 4185
Size: 30 374 30 1456 30 1227 30 287
Cuts at: 4185 4215 4486 4516 4615 4645 721
Size: 30 271 30 99 30 3755
Fragments arranged by size:
3755 1456 1227 374 287 271 99 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 371 415 1130 1133 1961 2071 2074 2101 2131
Size: 44 715 3 828 110 3 27 30
Cuts at: 2131 2197 2516 2647 3271 3699 3709 3912 4147
Size: 66 319 131 624 428 10 203 235
Cuts at: 4147 4494 371
Size: 347 3556
Fragments arranged by size:
3556 828 715 624 428 347 319 235
203 131 110 66 44 30 27 10
3 3
BseYI C'CCAG_C
Cuts at: 963 1099 2256 2888 3249 3346 3386 3497 3534
Size: 136 1157 632 361 97 40 111 37
Cuts at: 3534 3552 3648 3669 3969 4015 4049 4172 5104
Size: 18 96 21 300 46 34 123 932
Cuts at: 5104 5862 6527 6935 963
Size: 758 665 408 1707
Fragments arranged by size:
1707 1157 932 758 665 632 408 361
300 136 123 111 97 96 46 40
37 34 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 629 937 1091 4419 119
Size: 510 308 154 3328 3379
Fragments arranged by size:
3379 3328 510 308 154
BsiEI CG_ry'CG
Cuts at: 104 1740 5221 5267 6497 104
Size: 1636 3481 46 1230 1286
Fragments arranged by size:
3481 1636 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 428 538 709 739 2358 3355 4693 5471 5661
Size: 110 171 30 1619 997 1338 778 190
Cuts at: 5661 6521 428
Size: 860 1586
Fragments arranged by size:
1619 1586 1338 997 860 778 190 171
110 30
BsmI GAATG_Cn'
Cuts at: 1896 3306 1896
Size: 1410 6269
Bsp1286I G_dGCh'C
Cuts at: 428 538 666 709 739 842 874 889 1013
Size: 110 128 43 30 103 32 15 124
Cuts at: 1013 1085 1346 1606 1650 2158 2358 2519 2645
Size: 72 261 260 44 508 200 161 126
Cuts at: 2645 2688 2888 2986 3355 3498 3869 4187 4396
Size: 43 200 98 369 143 371 318 209
Cuts at: 4396 4405 4430 4546 4693 5304 5397 5471 5661
Size: 9 25 116 147 611 93 74 190
Cuts at: 5661 5723 6521 428
Size: 62 798 1586
Fragments arranged by size:
1586 798 611 508 371 369 318 261
260 209 200 200 190 161 147 143
128 126 124 116 110 103 98 93
74 72 62 44 43 43 32 30
25 15 9
BspHI T'CATG_A
Cuts at: 3194 6111 3194
Size: 2917 4762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 959 2856 3579 3591 4505 4729 4943 5245 5626
Size: 1897 723 12 914 224 214 302 381
Cuts at: 5626 959
Size: 3012
Fragments arranged by size:
3012 1897 914 723 381 302 224 214
12
BsrBI CCG'CTC
Cuts at: 50 431 1179 1191 5971 6025 7509 7674 50
Size: 381 748 12 4780 54 1484 165 55
Fragments arranged by size:
4780 1484 748 381 165 55 54 12
BsrDI GCAATG_nn'
Cuts at: 1246 4003 4417 5591 1246
Size: 2757 414 1174 3334
Fragments arranged by size:
3334 2757 1174 414
BsrFI r'CCGG_y
Cuts at: 658 1735 1742 3630 5677 5858 658
Size: 1077 7 1888 2047 181 2479
Fragments arranged by size:
2479 2047 1888 1077 181 7
BssSI C'ACGA_G
Cuts at: 1450 3305 5950 6658 1450
Size: 1855 2645 708 2471
Fragments arranged by size:
2645 2471 1855 708
BstAPI GCAn_nnn'nTGC
Cuts at: 713 1044 1341 1979 3398 5056 713
Size: 331 297 638 1419 1658 3336
Fragments arranged by size:
3336 1658 1419 638 331 297
BstBI TT'CG_AA
Cuts at: 1515 2709 6040 1515
Size: 1194 3331 3154
Fragments arranged by size:
3331 3154 1194
BstXI CCAn_nnnn'nTGG
Cuts at: 3756 3769 3756
Size: 13 7666
BstYI r'GATC_y
Cuts at: 1 252 749 2373 2567 3042 4369 4704 5171
Size: 251 497 1624 194 475 1327 335 467
Cuts at: 5171 5194 5529 5775 6081 6093 6179 7231 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1624 1327 1052 497 475 467 449 335
335 306 251 246 194 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 303 1370 1628 2150 3542 3711 303
Size: 1067 258 522 1392 169 4271
Fragments arranged by size:
4271 1392 1067 522 258 169
BtgI C'CryG_G
Cuts at: 392 1199 4289 392
Size: 807 3090 3782
Fragments arranged by size:
3782 3090 807
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4288 5629 5810 5973 4288
Size: 1341 181 163 5994
Fragments arranged by size:
5994 1341 181 163
BtsI GCAGTG_nn'
Cuts at: 677 1018 1268 2493 2747 2864 3372 4443 4571
Size: 341 250 1225 254 117 508 1071 128
Cuts at: 4571 4922 677
Size: 351 3434
Fragments arranged by size:
3434 1225 1071 508 351 341 254 250
128 117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 474 509 926 961 4400 4435 474
Size: 35 417 35 3439 35 3718
Fragments arranged by size:
3718 3439 417 35 35 35
DraI TTT'AAA
Cuts at: 4678 4678
Size: 7679
DraIII CAC_nnn'GTG
Cuts at: 542 2971 542
Size: 2429 5250
DrdI GACnn_nn'nnGTC
Cuts at: 1844 5385 6729 1844
Size: 3541 1344 2794
Fragments arranged by size:
3541 2794 1344
EaeI y'GGCC_r
Cuts at: 162 315 1202 1737 3470 5264 5438 5829 5856
Size: 153 887 535 1733 1794 174 391 27
Cuts at: 5856 7201 162
Size: 1345 640
Fragments arranged by size:
1794 1733 1345 887 640 535 391 174
153 27
EagI C'GGCC_G
Cuts at: 1737 5264 1737
Size: 3527 4152
EarI CTCTTCn'nnn_
Cuts at: 263 401 830 979 1426 1649 1685 2048 2060
Size: 138 429 149 447 223 36 363 12
Cuts at: 2060 2078 2087 2225 2972 3499 5702 5912 263
Size: 18 9 138 747 527 2203 210 2030
Fragments arranged by size:
2203 2030 747 527 447 429 363 223
210 149 138 138 36 18 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 374 5887 6629 6775 7489 374
Size: 5513 742 146 714 564
Fragments arranged by size:
5513 742 714 564 146
EcoICRI GAG'CTC
Cuts at: 426 737 4691 426
Size: 311 3954 3414
Fragments arranged by size:
3954 3414 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 326 389 431 766 1015 1673 1689 1734
Size: 12 63 42 335 249 658 16 45
Cuts at: 1734 2123 2156 2249 2277 2993 3116 3137 3208
Size: 389 33 93 28 716 123 21 71
Cuts at: 3208 3523 3870 4144 4211 5504 5936 6289 6951
Size: 315 347 274 67 1293 432 353 662
Cuts at: 6951 314
Size: 1042
Fragments arranged by size:
1293 1042 716 662 658 432 389 353
347 335 315 274 249 123 93 71
67 63 45 42 33 28 21 16
12
EcoNI CCTnn'n_nnAGG
Cuts at: 1631 2053 2141 3848 4226 1631
Size: 422 88 1707 378 5084
Fragments arranged by size:
5084 1707 422 378 88
EcoO109I rG'GnC_Cy
Cuts at: 223 288 885 886 1081 1225 1374 1406 1640
Size: 65 597 1 195 144 149 32 234
Cuts at: 1640 2127 2146 2154 2301 2684 2885 2982 3112
Size: 487 19 8 147 383 201 97 130
Cuts at: 3112 3164 3372 3564 3853 3910 4061 4347 4542
Size: 52 208 192 289 57 151 286 195
Cuts at: 4542 4818 223
Size: 276 3084
Fragments arranged by size:
3084 597 487 383 289 286 276 234
208 201 195 195 192 151 149 147
144 130 97 65 57 52 32 19
8 1
EcoRI G'AATT_C
Cuts at: 4683 4683
Size: 7679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 570 5698 570
Size: 5128 2551
FspI TGC'GCA
Cuts at: 2539 5460 2539
Size: 2921 4758
HaeII r_GCGC'y
Cuts at: 1811 2535 3159 3631 5113 5361 6591 7362 1811
Size: 724 624 472 1482 248 1230 771 2128
Fragments arranged by size:
2128 1482 1230 771 724 624 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 768 800 1915 1947 2611 2643 3868
Size: 32 450 32 1115 32 664 32 1225
Cuts at: 3868 3900 5521 5551 5553 5583 286
Size: 32 1621 30 2 30 2382
Fragments arranged by size:
2382 1621 1225 1115 664 450 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4718 4718
Size: 7679
HindIII A'AGCT_T
Cuts at: 3002 3212 3002
Size: 210 7469
KasI G'GCGC_C
Cuts at: 3627 5109 5357 3627
Size: 1482 248 5949
Fragments arranged by size:
5949 1482 248
KpnI G_GTAC'C
Cuts at: 4699 4699
Size: 7679
MluI A'CGCG_T
Cuts at: 7112 7112
Size: 7679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2621 4095 4658 6438 6622 2621
Size: 1474 563 1780 184 3678
Fragments arranged by size:
3678 1780 1474 563 184
MscI TGG'CCA
Cuts at: 164 317 1204 3472 5440 164
Size: 153 887 2268 1968 2403
Fragments arranged by size:
2403 2268 1968 887 153
MslI CAynn'nnrTG
Cuts at: 2267 3754 5668 5795 2267
Size: 1487 1914 127 4151
Fragments arranged by size:
4151 1914 1487 127
MspA1I CmG'CkG
Cuts at: 451 1044 1129 3153 3186 3346 3501 3669 4036
Size: 593 85 2024 33 160 155 168 367
Cuts at: 4036 4790 5104 5464 6248 6493 451
Size: 754 314 360 784 245 1637
Fragments arranged by size:
2024 1637 784 754 593 367 360 314
245 168 160 155 85 33
NaeI GCC'GGC
Cuts at: 1737 5860 1737
Size: 4123 3556
NarI GG'CG_CC
Cuts at: 3628 5110 5358 3628
Size: 1482 248 5949
Fragments arranged by size:
5949 1482 248
NcoI C'CATG_G
Cuts at: 392 1199 392
Size: 807 6872
NgoMIV G'CCGG_C
Cuts at: 1735 5858 1735
Size: 4123 3556
NheI G'CTAG_C
Cuts at: 1861 1861
Size: 7679
NspI r_CATG'y
Cuts at: 759 1462 2589 4457 4732 5232 6835 759
Size: 703 1127 1868 275 500 1603 1603
Fragments arranged by size:
1868 1603 1603 1127 703 500 275
PasI CC'CwG_GG
Cuts at: 2522 2719 3088 2522
Size: 197 369 7113
Fragments arranged by size:
7113 369 197
PciI A'CATG_T
Cuts at: 1458 6831 1458
Size: 5373 2306
PflMI CCAn_nnn'nTGG
Cuts at: 4449 4449
Size: 7679
PmeI GTTT'AAAC
Cuts at: 4678 4678
Size: 7679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6125 6157 6125
Size: 32 7647
PpuMI rG'GwC_Cy
Cuts at: 288 1640 2301 3112 3164 288
Size: 1352 661 811 52 4803
Fragments arranged by size:
4803 1352 811 661 52
PshAI GACnn'nnGTC
Cuts at: 3964 3964
Size: 7679
PsiI TTA'TAA
Cuts at: 587 587
Size: 7679
PspOMI G'GGCC_C
Cuts at: 885 1081 1602 2154 2684 2884 2982 4542 885
Size: 196 521 552 530 200 98 1560 4022
Fragments arranged by size:
4022 1560 552 530 521 200 196 98
PstI C_TGCA'G
Cuts at: 438 718 1275 1790 1975 2348 3039 3185 4726
Size: 280 557 515 185 373 691 146 1541
Cuts at: 4726 5411 438
Size: 685 2706
Fragments arranged by size:
2706 1541 691 685 557 515 373 280
185 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7679
PvuII CAG'CTG
Cuts at: 451 1044 1129 3186 3501 4036 5104 5464 451
Size: 593 85 2057 315 535 1068 360 2666
Fragments arranged by size:
2666 2057 1068 593 535 360 315 85
RsrII CG'GwC_CG
Cuts at: 5874 5874
Size: 7679
SacI G_AGCT'C
Cuts at: 428 739 4693 428
Size: 311 3954 3414
Fragments arranged by size:
3954 3414 311
SalI G'TCGA_C
Cuts at: 4716 4716
Size: 7679
SanDI GG'GwC_CC
Cuts at: 1640 1640
Size: 7679
SapI GCTCTTCn'nnn_
Cuts at: 830 1649 5702 5912 830
Size: 819 4053 210 2597
Fragments arranged by size:
4053 2597 819 210
SbfI CC_TGCA'GG
Cuts at: 4726 4726
Size: 7679
ScaI AGT'ACT
Cuts at: 7196 7196
Size: 7679
SfcI C'TryA_G
Cuts at: 33 434 714 1271 1786 1971 2344 3035 3159
Size: 401 280 557 515 185 373 691 124
Cuts at: 3159 3181 3331 4054 4722 5407 6375 6566 33
Size: 22 150 723 668 685 968 191 1146
Fragments arranged by size:
1146 968 723 691 685 668 557 515
401 373 280 191 185 150 124 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 1405 3722 1405
Size: 2317 5362
SfoI GGC'GCC
Cuts at: 3629 5111 5359 3629
Size: 1482 248 5949
Fragments arranged by size:
5949 1482 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7679
SmaI CCC'GGG
Cuts at: 2689 4701 2689
Size: 2012 5667
SmlI C'TyrA_G
Cuts at: 1024 1072 1441 2023 3478 3559 3937 4535 4834
Size: 48 369 582 1455 81 378 598 299
Cuts at: 4834 5749 6463 6725 1024
Size: 915 714 262 1978
Fragments arranged by size:
1978 1455 915 714 598 582 378 369
299 262 81 48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7679
SphI G_CATG'C
Cuts at: 4732 5232 4732
Size: 500 7179
SspI AAT'ATT
Cuts at: 570 570
Size: 7679
StuI AGG'CCT
Cuts at: 3716 3716
Size: 7679
StyI C'CwwG_G
Cuts at: 392 842 944 1160 1199 3311 3869 4813 5167
Size: 450 102 216 39 2112 558 944 354
Cuts at: 5167 392
Size: 2904
Fragments arranged by size:
2904 2112 944 558 450 354 216 102
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 574 684 2982 3726 4563 5262 5395 5854
Size: 421 110 2298 744 837 699 133 459
Cuts at: 5854 6063 153
Size: 209 1769
Fragments arranged by size:
2298 1769 837 744 699 459 421 209
133 110
TatI w'GTAC_w
Cuts at: 1319 7194 1319
Size: 5875 1804
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1125 1157 4185 4217 4354 4386 4486 4518 4615
Size: 32 3028 32 137 32 100 32 97
Cuts at: 4615 4647 7196 7228 1125
Size: 32 2549 32 1576
Fragments arranged by size:
3028 2549 1576 137 100 97 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3844 3880 5476 3844
Size: 36 1596 6047
Fragments arranged by size:
6047 1596 36
XbaI T'CTAG_A
Cuts at: 1287 4710 1287
Size: 3423 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 495 1167 4078 5174 495
Size: 672 2911 1096 3000
Fragments arranged by size:
3000 2911 1096 672
XmaI C'CCGG_G
Cuts at: 2687 4699 2687
Size: 2012 5667
XmnI GAAnn'nnTTC
Cuts at: 1420 1420
Size: 7679
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI AscI AvrII BaeI
BfrBI BmgBI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI NsiI PacI PfoI PmlI PspXI PsrI
SacII SexAI SgrAI SnaBI SrfI SwaI XhoI ZraI