Restriction map for
pF1KA1763
(Circular) (Six-base) MAPSORT of: pF1KA1763.seq Check: 609 from: 1 to: 7037
pF1KA1763 7037 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1429 3574 1429
Size: 2145 4892
AccI GT'mk_AC
Cuts at: 3953 4075 3953
Size: 122 6915
Acc65I G'GTAC_C
Cuts at: 4053 4053
Size: 7037
AclI AA'CG_TT
Cuts at: 6877 6877
Size: 7037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 1327 1707 1717 1901 2089 3664 4862 5294
Size: 1105 380 10 184 188 1575 1198 432
Cuts at: 5294 6041 6309 222
Size: 747 268 950
Fragments arranged by size:
1575 1198 1105 950 747 432 380 268
188 184 10
AflII C'TTAA_G
Cuts at: 1390 1390
Size: 7037
AflIII A'CryG_T
Cuts at: 3693 4389 5493 6470 3693
Size: 696 1104 977 4260
Fragments arranged by size:
4260 1104 977 696
AgeI A'CCGG_T
Cuts at: 5425 5425
Size: 7037
AleI CACnn'nnGTG
Cuts at: 385 1502 385
Size: 1117 5920
AlwNI CAG_nnn'CTG
Cuts at: 494 1060 3676 4342 5909 494
Size: 566 2616 666 1567 1622
Fragments arranged by size:
2616 1622 1567 666 566
ApaLI G'TGCA_C
Cuts at: 5807 5807
Size: 7037
ApoI r'AATT_y
Cuts at: 186 739 1006 1089 2484 3235 3508 4041 6390
Size: 553 267 83 1395 751 273 533 2349
Cuts at: 6390 6646 186
Size: 256 577
Fragments arranged by size:
2349 1395 751 577 553 533 273 267
256 83
AseI AT'TA_AT
Cuts at: 20 842 20
Size: 822 6215
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7037
AvaI C'yCGr_G
Cuts at: 4057 4057
Size: 7037
BamHI G'GATC_C
Cuts at: 1612 4062 1612
Size: 2450 4587
BanI G'GyrC_C
Cuts at: 1573 2735 4053 4467 4715 4750 1573
Size: 1162 1318 414 248 35 3860
Fragments arranged by size:
3860 1318 1162 414 248 35
BanII G_rGCy'C
Cuts at: 257 715 1237 2952 3286 3800 4051 5081 257
Size: 458 522 1715 334 514 251 1030 2213
Fragments arranged by size:
2213 1715 1030 522 514 458 334 251
BbeI G_GCGC'C
Cuts at: 4471 4719 4471
Size: 248 6789
BbvCI CC'TCA_GC
Cuts at: 3962 3962
Size: 7037
BciVI GTATCCnnnnn_n'
Cuts at: 1372 4935 5696 7037 1372
Size: 3563 761 1341 1372
Fragments arranged by size:
3563 1372 1341 761
BclI T'GATC_A
Cuts at: 696 912 3230 3311 4557 696
Size: 216 2318 81 1246 3176
Fragments arranged by size:
3176 2318 1246 216 81
BglII A'GATC_T
Cuts at: 1686 4552 6589 1686
Size: 2866 2037 2134
Fragments arranged by size:
2866 2134 2037
BlpI GC'TnA_GC
Cuts at: 3217 3625 4149 3217
Size: 408 524 6105
Fragments arranged by size:
6105 524 408
Bme1580I G_kGCm'C
Cuts at: 4662 4755 5811 4662
Size: 93 1056 5888
Fragments arranged by size:
5888 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 429 1418 1797 2250 2455 4249 4330 4665 6809
Size: 989 379 453 205 1794 81 335 2144
Cuts at: 6809 429
Size: 657
Fragments arranged by size:
2144 1794 989 657 453 379 335 205
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 148 844 2658 5404 148
Size: 696 1814 2746 1781
Fragments arranged by size:
2746 1814 1781 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 529 1221 1289 1830 2945 3162 243
Size: 286 692 68 541 1115 217 4118
Fragments arranged by size:
4118 1115 692 541 286 217 68
Bpu10I CC'TnA_GC
Cuts at: 709 1553 3962 709
Size: 844 2409 3784
Fragments arranged by size:
3784 2409 844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 380 1521 4213 5092 5584 5882 118
Size: 262 1141 2692 879 492 298 1273
Fragments arranged by size:
2692 1273 1141 879 492 298 262
BsaI GGTCTCn'nnnn_
Cuts at: 323 2912 6741 323
Size: 2589 3829 619
Fragments arranged by size:
3829 2589 619
BsaAI yAC'GTr
Cuts at: 3633 3696 4390 3633
Size: 63 694 6280
Fragments arranged by size:
6280 694 63
BsaBI GATnn'nnATC
Cuts at: 6 3862 4551 6588 6
Size: 3856 689 2037 455
Fragments arranged by size:
3856 2037 689 455
BsaHI Gr'CG_yC
Cuts at: 4468 4716 5418 6914 4468
Size: 248 702 1496 4591
Fragments arranged by size:
4591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2333 4226 4747 5425 5699 5846 6257 2333
Size: 1893 521 678 274 147 411 3113
Fragments arranged by size:
3113 1893 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3166 3196 3166
Size: 30 7007
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1288 1291 1357 1529 1865 1288
Size: 3 66 172 336 6460
Fragments arranged by size:
6460 336 172 66 3
BseYI C'CCAG_C
Cuts at: 181 1545 2068 3661 3719 4462 5220 5797 6293
Size: 1364 523 1593 58 743 758 577 496
Cuts at: 6293 181
Size: 925
Fragments arranged by size:
1593 1364 925 758 743 577 523 496
58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2722 2989 3048 3536 3560 3640 2144
Size: 578 267 59 488 24 80 5541
Fragments arranged by size:
5541 578 488 267 80 59 24
BsiEI CG_ry'CG
Cuts at: 104 4579 4625 5833 104
Size: 4475 46 1208 1308
Fragments arranged by size:
4475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 935 1237 1816 2132 3286 4051 4829 5019
Size: 678 302 579 316 1154 765 778 190
Cuts at: 5019 5811 257
Size: 792 1483
Fragments arranged by size:
1483 1154 792 778 765 678 579 316
302 190
BsmI GAATG_Cn'
Cuts at: 620 924 1467 2424 2900 620
Size: 304 543 957 476 4757
Fragments arranged by size:
4757 957 543 476 304
Bsp1286I G_dGCh'C
Cuts at: 257 715 935 1237 1816 2132 2952 3286 3800
Size: 458 220 302 579 316 820 334 514
Cuts at: 3800 4051 4662 4755 4829 5019 5081 5811 257
Size: 251 611 93 74 190 62 730 1483
Fragments arranged by size:
1483 820 730 611 579 514 458 334
316 302 251 220 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6257 6257
Size: 7037
BspHI T'CATG_A
Cuts at: 1016 6213 1016
Size: 5197 1840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1429 3574 4087 4301 4603 4984 1429
Size: 2145 513 214 302 381 3482
Fragments arranged by size:
3482 2145 513 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5329 5383 6867 7032 50
Size: 5279 54 1484 165 55
Fragments arranged by size:
5279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1169 2988 3024 3314 4949 1169
Size: 1819 36 290 1635 3257
Fragments arranged by size:
3257 1819 1635 290 36
BsrFI r'CCGG_y
Cuts at: 5035 5216 5425 5035
Size: 181 209 6647
Fragments arranged by size:
6647 209 181
BssSI C'ACGA_G
Cuts at: 5308 5666 5308
Size: 358 6679
BstAPI GCAn_nnn'nTGC
Cuts at: 930 4414 930
Size: 3484 3553
BstBI TT'CG_AA
Cuts at: 1302 5398 1302
Size: 4096 2941
BstEII G'GTnAC_C
Cuts at: 378 423 378
Size: 45 6992
BstXI CCAn_nnnn'nTGG
Cuts at: 3714 3714
Size: 7037
BstYI r'GATC_y
Cuts at: 1 112 1612 1686 3121 4062 4529 4552 4887
Size: 111 1500 74 1435 941 467 23 335
Cuts at: 4887 5133 6145 6231 6243 6589 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1500 1435 1012 941 467 449 346 335
246 111 86 74 23 12
Bsu36I CC'TnA_GG
Cuts at: 227 227
Size: 7037
BtgI C'CryG_G
Cuts at: 107 2535 107
Size: 2428 4609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4987 5168 5331 4987
Size: 181 163 6693
Fragments arranged by size:
6693 181 163
BtsI GCAGTG_nn'
Cuts at: 805 1252 1468 3623 3878 4280 805
Size: 447 216 2155 255 402 3562
Fragments arranged by size:
3562 2155 447 402 255 216
DraI TTT'AAA
Cuts at: 3393 4036 3393
Size: 643 6394
DraIII CAC_nnn'GTG
Cuts at: 1636 2742 1636
Size: 1106 5931
DrdI GACnn_nn'nnGTC
Cuts at: 4743 5601 4743
Size: 858 6179
EaeI y'GGCC_r
Cuts at: 389 1145 1518 2087 2870 4622 4796 5187 5214
Size: 756 373 569 783 1752 174 391 27
Cuts at: 5214 6559 389
Size: 1345 867
Fragments arranged by size:
1752 1345 867 783 756 569 391 373
174 27
EagI C'GGCC_G
Cuts at: 4622 4622
Size: 7037
EarI CTCTTCn'nnn_
Cuts at: 291 813 2860 5060 5270 291
Size: 522 2047 2200 210 2058
Fragments arranged by size:
2200 2058 2047 522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5245 5555 5701 6847 5245
Size: 310 146 1146 5435
Fragments arranged by size:
5435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 255 1235 3284 4049 255
Size: 980 2049 765 3243
Fragments arranged by size:
3243 2049 980 765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 243 529 1221 1289 1327 1707 1717 1830
Size: 21 286 692 68 38 380 10 113
Cuts at: 1830 1901 2089 2945 3162 3664 4862 5294 6041
Size: 71 188 856 217 502 1198 432 747
Cuts at: 6041 6309 222
Size: 268 950
Fragments arranged by size:
1198 950 856 747 692 502 432 380
286 268 217 188 113 71 68 38
21 10
EcoO109I rG'GnC_Cy
Cuts at: 1549 1709 2449 4176 1549
Size: 160 740 1727 4410
Fragments arranged by size:
4410 1727 740 160
EcoRI G'AATT_C
Cuts at: 186 1006 2484 3508 4041 186
Size: 820 1478 1024 533 3182
Fragments arranged by size:
3182 1478 1024 820 533
EcoRV GAT'ATC
Cuts at: 1134 1134
Size: 7037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1515 2398 3319 5056 1515
Size: 883 921 1737 3496
Fragments arranged by size:
3496 1737 921 883
FspI TGC'GCA
Cuts at: 3590 4818 3590
Size: 1228 5809
HaeII r_GCGC'y
Cuts at: 4471 4719 5741 6720 4471
Size: 248 1022 979 4788
Fragments arranged by size:
4788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 785 817 1118 1150 3215 3247 3724
Size: 32 65 32 301 32 2065 32 477
Cuts at: 3724 3756 4879 4909 4911 4941 688
Size: 32 1123 30 2 30 2784
Fragments arranged by size:
2784 2065 1123 477 301 65 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 960 3381 4076 960
Size: 2421 695 3921
Fragments arranged by size:
3921 2421 695
HindIII A'AGCT_T
Cuts at: 1605 3264 1605
Size: 1659 5378
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 7037
KasI G'GCGC_C
Cuts at: 4467 4715 4467
Size: 248 6789
KpnI G_GTAC'C
Cuts at: 4057 4057
Size: 7037
MfeI C'AATT_G
Cuts at: 2319 2319
Size: 7037
MluI A'CGCG_T
Cuts at: 6470 6470
Size: 7037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 2671 5708 5892 440
Size: 2231 3037 184 1585
Fragments arranged by size:
3037 2231 1585 184
MscI TGG'CCA
Cuts at: 391 1147 2089 4798 391
Size: 756 942 2709 2630
Fragments arranged by size:
2709 2630 942 756
MslI CAynn'nnrTG
Cuts at: 385 992 1174 1502 3557 5026 5153 385
Size: 607 182 328 2055 1469 127 2269
Fragments arranged by size:
2269 2055 1469 607 328 182 127
MspA1I CmG'CkG
Cuts at: 385 1347 1673 2790 2818 3710 4148 4462 4822
Size: 962 326 1117 28 892 438 314 360
Cuts at: 4822 5835 6080 385
Size: 1013 245 1342
Fragments arranged by size:
1342 1117 1013 962 892 438 360 326
314 245 28
NaeI GCC'GGC
Cuts at: 5218 5218
Size: 7037
NarI GG'CG_CC
Cuts at: 4468 4716 4468
Size: 248 6789
NcoI C'CATG_G
Cuts at: 107 107
Size: 7037
NdeI CA'TA_TG
Cuts at: 2064 2064
Size: 7037
NgoMIV G'CCGG_C
Cuts at: 5216 5216
Size: 7037
NspI r_CATG'y
Cuts at: 2830 4090 4590 5497 2830
Size: 1260 500 907 4370
Fragments arranged by size:
4370 1260 907 500
PasI CC'CwG_GG
Cuts at: 894 894
Size: 7037
PciI A'CATG_T
Cuts at: 5493 5493
Size: 7037
PflMI CCAn_nnn'nTGG
Cuts at: 945 1060 1636 2068 945
Size: 115 576 432 5914
Fragments arranged by size:
5914 576 432 115
PfoI T'CCnGG_A
Cuts at: 273 1237 1807 273
Size: 964 570 5503
Fragments arranged by size:
5503 964 570
PmeI GTTT'AAAC
Cuts at: 4036 4036
Size: 7037
PmlI CAC'GTG
Cuts at: 3633 3696 3633
Size: 63 6974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6176 6208 6176
Size: 32 7005
PpuMI rG'GwC_Cy
Cuts at: 1709 1709
Size: 7037
PsiI TTA'TAA
Cuts at: 1121 2372 2441 1121
Size: 1251 69 5717
Fragments arranged by size:
5717 1251 69
PstI C_TGCA'G
Cuts at: 2915 3618 3831 4084 4769 2915
Size: 703 213 253 685 5183
Fragments arranged by size:
5183 703 685 253 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7037
PvuII CAG'CTG
Cuts at: 1347 2790 3710 4462 4822 1347
Size: 1443 920 752 360 3562
Fragments arranged by size:
3562 1443 920 752 360
RsrII CG'GwC_CG
Cuts at: 5232 5232
Size: 7037
SacI G_AGCT'C
Cuts at: 257 1237 3286 4051 257
Size: 980 2049 765 3243
Fragments arranged by size:
3243 2049 980 765
SalI G'TCGA_C
Cuts at: 4074 4074
Size: 7037
SanDI GG'GwC_CC
Cuts at: 1709 1709
Size: 7037
SapI GCTCTTCn'nnn_
Cuts at: 813 2860 5060 5270 813
Size: 2047 2200 210 2580
Fragments arranged by size:
2580 2200 2047 210
SbfI CC_TGCA'GG
Cuts at: 4084 4084
Size: 7037
ScaI AGT'ACT
Cuts at: 4024 6554 4024
Size: 2530 4507
SfcI C'TryA_G
Cuts at: 33 537 687 2911 3614 3827 4080 4765 5758
Size: 504 150 2224 703 213 253 685 993
Cuts at: 5758 5949 33
Size: 191 1121
Fragments arranged by size:
2224 1121 993 703 685 504 253 213
191 150
SfoI GGC'GCC
Cuts at: 4469 4717 4469
Size: 248 6789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7037
SmaI CCC'GGG
Cuts at: 4059 4059
Size: 7037
SmlI C'TyrA_G
Cuts at: 133 395 1390 1500 4192 5107 5599 5861 133
Size: 262 995 110 2692 915 492 262 1309
Fragments arranged by size:
2692 1309 995 915 492 262 262 110
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7037
SphI G_CATG'C
Cuts at: 4090 4590 4090
Size: 500 6537
StuI AGG'CCT
Cuts at: 2770 2770
Size: 7037
StyI C'CwwG_G
Cuts at: 107 360 599 1713 4171 4525 107
Size: 253 239 1114 2458 354 2619
Fragments arranged by size:
2619 2458 1114 354 253 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 2002 2724 2728 2851 2894 3683 3715 4620
Size: 1591 722 4 123 43 789 32 905
Cuts at: 4620 4753 5212 5421 411
Size: 133 459 209 2027
Fragments arranged by size:
2027 1591 905 789 722 459 209 133
123 43 32 4
TatI w'GTAC_w
Cuts at: 533 2272 4022 6552 533
Size: 1739 1750 2530 1018
Fragments arranged by size:
2530 1750 1739 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1055 1087 1710 1742 6554 6586 1055
Size: 32 623 32 4812 32 1506
Fragments arranged by size:
4812 1506 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3676 3702 4834 3676
Size: 26 1132 5879
Fragments arranged by size:
5879 1132 26
XbaI T'CTAG_A
Cuts at: 4068 4068
Size: 7037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 4532 876
Size: 3656 3381
XmaI C'CCGG_G
Cuts at: 4057 4057
Size: 7037
XmnI GAAnn'nnTTC
Cuts at: 675 1914 1964 2381 675
Size: 1239 50 417 5331
Fragments arranged by size:
5331 1239 417 50
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoNI FseI
FspAI NheI NotI NruI NsiI PacI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI