Restriction map for
pF1KA1773
(Circular) (Six-base) MAPSORT of: pF1KA1773.seq Check: 2721 from: 1 to: 13007
pF1KA1773 13007 bp
With 182 enzymes: SgfI *
April 9, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 924 1524 1893 3642 4026 4365 6672 7236 9366
Size: 600 369 1749 384 339 2307 564 2130
Cuts at: 9366 9529 924
Size: 163 4402
Fragments arranged by size:
4402 2307 2130 1749 600 564 384 369
339 163
AatII G_ACGT'C
Cuts at: 3525 5033 3525
Size: 1508 11499
AccI GT'mk_AC
Cuts at: 6773 10045 6773
Size: 3272 9735
Acc65I G'GTAC_C
Cuts at: 561 3783 10023 561
Size: 3222 6240 3545
Fragments arranged by size:
6240 3545 3222
AclI AA'CG_TT
Cuts at: 12847 12847
Size: 13007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 3337 3572 3734 6018 6092 7056 7356 7604
Size: 518 235 162 2284 74 964 300 248
Cuts at: 7604 10832 11264 11617 12279 2819
Size: 3228 432 353 662 3547
Fragments arranged by size:
3547 3228 2284 964 662 518 432 353
300 248 235 162 74
AfeI AGC'GCT
Cuts at: 4469 4596 4907 4469
Size: 127 311 12569
Fragments arranged by size:
12569 311 127
AflIII A'CryG_T
Cuts at: 4758 4842 10359 12159 12440 4758
Size: 84 5517 1800 281 5325
Fragments arranged by size:
5517 5325 1800 281 84
AleI CACnn'nnGTG
Cuts at: 2215 2530 4213 5935 6142 6262 7408 7568 8248
Size: 315 1683 1722 207 120 1146 160 680
Cuts at: 8248 8278 9727 2215
Size: 30 1449 5495
Fragments arranged by size:
5495 1722 1683 1449 1146 680 315 207
160 120 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2233 2734 5125 7779 2233
Size: 501 2391 2654 7461
Fragments arranged by size:
7461 2654 2391 501
AlwNI CAG_nnn'CTG
Cuts at: 622 721 825 3165 3334 3645 3694 3757 4029
Size: 99 104 2340 169 311 49 63 272
Cuts at: 4029 4108 4132 5010 5917 5941 7155 7633 7825
Size: 79 24 878 907 24 1214 478 192
Cuts at: 7825 7873 7942 8362 8494 8857 9022 9072 9151
Size: 48 69 420 132 363 165 50 79
Cuts at: 9151 9220 9451 9964 10312 11750 622
Size: 69 231 513 348 1438 1879
Fragments arranged by size:
2340 1879 1438 1214 907 878 513 478
420 363 348 311 272 231 192 169
165 132 104 99 79 79 69 69
63 50 49 48 24 24
ApaI G_GGCC'C
Cuts at: 819 2057 2981 3199 4793 5232 5924 6674 7061
Size: 1238 924 218 1594 439 692 750 387
Cuts at: 7061 7115 8997 9443 819
Size: 54 1882 446 4383
Fragments arranged by size:
4383 1882 1594 1238 924 750 692 446
439 387 218 54
ApaLI G'TGCA_C
Cuts at: 2022 4196 7510 8159 11845 2022
Size: 2174 3314 649 3686 3184
Fragments arranged by size:
3686 3314 3184 2174 649
ApoI r'AATT_y
Cuts at: 10011 12360 12616 10011
Size: 2349 256 10402
Fragments arranged by size:
10402 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 13007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 13007
AvaI C'yCGr_G
Cuts at: 545 1598 2429 2606 2741 3017 4231 4814 5588
Size: 1053 831 177 135 276 1214 583 774
Cuts at: 5588 6200 6314 7545 7751 7916 9785 10027 545
Size: 612 114 1231 206 165 1869 242 3525
Fragments arranged by size:
3525 1869 1231 1214 1053 831 774 612
583 276 242 206 177 165 135 114
AvrII C'CTAG_G
Cuts at: 8979 8979
Size: 13007
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 701 734 701
Size: 33 12974
BamHI G'GATC_C
Cuts at: 1602 2251 2601 3429 3834 5196 7065 10032 1602
Size: 649 350 828 405 1362 1869 2967 4577
Fragments arranged by size:
4577 2967 1869 1362 828 649 405 350
BanI G'GyrC_C
Cuts at: 216 223 478 561 583 1615 1651 1876 2517
Size: 7 255 83 22 1032 36 225 641
Cuts at: 2517 2524 2571 3142 3783 4098 4222 4237 5043
Size: 7 47 571 641 315 124 15 806
Cuts at: 5043 5385 5530 5697 5791 6088 6472 7324 8077
Size: 342 145 167 94 297 384 852 753
Cuts at: 8077 8175 8550 8581 8635 8733 8895 9614 10023
Size: 98 375 31 54 98 162 719 409
Cuts at: 10023 10437 10685 10720 216
Size: 414 248 35 2503
Fragments arranged by size:
2503 1032 852 806 753 719 641 641
571 414 409 384 375 342 315 297
255 248 225 167 162 145 124 98
98 94 83 54 47 36 35 31
22 15 7 7
BanII G_rGCy'C
Cuts at: 157 819 1125 1141 1550 1835 2057 2862 2981
Size: 662 306 16 409 285 222 805 119
Cuts at: 2981 3031 3199 3673 4165 4184 4460 4793 5232
Size: 50 168 474 492 19 276 333 439
Cuts at: 5232 5290 5825 5924 6674 6871 6886 6956 6985
Size: 58 535 99 750 197 15 70 29
Cuts at: 6985 7061 7115 7485 8054 8074 8709 8997 9443
Size: 76 54 370 569 20 635 288 446
Cuts at: 9443 9487 9578 10021 11051 157
Size: 44 91 443 1030 2113
Fragments arranged by size:
2113 1030 805 750 662 635 569 535
492 474 446 443 439 409 370 333
306 288 285 276 222 197 168 119
99 91 76 70 58 54 50 44
29 20 19 16 15
BbeI G_GCGC'C
Cuts at: 1655 5047 5795 10441 10689 1655
Size: 3392 748 4646 248 3973
Fragments arranged by size:
4646 3973 3392 748 248
BbsI GAAGACnn'nnnn_
Cuts at: 2807 3560 4701 4734 7592 7689 9636 2807
Size: 753 1141 33 2858 97 1947 6178
Fragments arranged by size:
6178 2858 1947 1141 753 97 33
BbvCI CC'TCA_GC
Cuts at: 1469 1733 2221 2393 4047 4133 4334 5575 7721
Size: 264 488 172 1654 86 201 1241 2146
Cuts at: 7721 8800 9011 9846 9939 9945 1469
Size: 1079 211 835 93 6 4531
Fragments arranged by size:
4531 2146 1654 1241 1079 835 488 264
211 201 172 93 86 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 283 317 2635 2669 8290 8324 9340
Size: 34 159 34 2318 34 5621 34 1016
Cuts at: 9340 9374 90
Size: 34 3723
Fragments arranged by size:
5621 3723 2318 1016 159 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10905 11961 13007 10905
Size: 1056 1046 10905
Fragments arranged by size:
10905 1056 1046
BclI T'GATC_A
Cuts at: 6266 8208 9245 10527 6266
Size: 1942 1037 1282 8746
Fragments arranged by size:
8746 1942 1282 1037
BglI GCCn_nnn'nGGC
Cuts at: 1937 2359 2976 3242 4788 8009 9274 9559 9833
Size: 422 617 266 1546 3221 1265 285 274
Cuts at: 9833 1937
Size: 5111
Fragments arranged by size:
5111 3221 1546 1265 617 422 285 274
266
BglII A'GATC_T
Cuts at: 4074 6821 10522 12559 4074
Size: 2747 3701 2037 4522
Fragments arranged by size:
4522 3701 2747 2037
BlpI GC'TnA_GC
Cuts at: 4591 4946 6293 6489 6885 7045 7964 9607 10119
Size: 355 1347 196 396 160 919 1643 512
Cuts at: 10119 4591
Size: 7479
Fragments arranged by size:
7479 1643 1347 919 512 396 355 196
160
Bme1580I G_kGCm'C
Cuts at: 376 819 1879 2026 2057 2196 2235 2430 2723
Size: 443 1060 147 31 139 39 195 293
Cuts at: 2723 2981 3199 4200 4240 4793 5232 5370 5924
Size: 258 218 1001 40 553 439 138 554
Cuts at: 5924 6674 7061 7115 7514 7758 8163 8423 8576
Size: 750 387 54 399 244 405 260 153
Cuts at: 8576 8586 8736 8997 9443 9619 9758 10632 10725
Size: 10 150 261 446 176 139 874 93
Cuts at: 10725 11849 376
Size: 1124 1534
Fragments arranged by size:
1534 1124 1060 1001 874 750 554 553
446 443 439 405 399 387 293 261
260 258 244 218 195 176 153 150
147 139 139 138 93 54 40 39
31 10
BmgBI CAC'GTC
Cuts at: 834 1491 4521 5139 834
Size: 657 3030 618 8702
Fragments arranged by size:
8702 3030 657 618
BmrI ACTGGGnnnn_n'
Cuts at: 746 2417 2686 7695 7768 8896 9082 9506 9603
Size: 1671 269 5009 73 1128 186 424 97
Cuts at: 9603 10219 10300 10635 12779 746
Size: 616 81 335 2144 974
Fragments arranged by size:
5009 2144 1671 1128 974 616 424 335
269 186 97 81 73
BmtI G_CTAG'C
Cuts at: 6323 6323
Size: 13007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 1155 3915 5844 9439 11374 777
Size: 378 2760 1929 3595 1935 2410
Fragments arranged by size:
3595 2760 2410 1935 1929 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 619 689 698 706 802 2401 2716 3025
Size: 107 70 9 8 96 1599 315 309
Cuts at: 3025 3031 3268 3608 3785 4006 4922 5401 5540
Size: 6 237 340 177 221 916 479 139
Cuts at: 5540 5648 5780 5839 5852 6734 7469 7639 8252
Size: 108 132 59 13 882 735 170 613
Cuts at: 8252 8540 8827 9173 9991 512
Size: 288 287 346 818 3528
Fragments arranged by size:
3528 1599 916 882 818 735 613 479
346 340 315 309 288 287 237 221
177 170 139 132 108 107 96 70
59 13 9 8 6
Bpu10I CC'TnA_GC
Cuts at: 566 722 1130 1348 1469 1544 1733 2221 2393
Size: 156 408 218 121 75 189 488 172
Cuts at: 2393 4047 4133 4334 5575 5926 7721 8034 8073
Size: 1654 86 201 1241 351 1795 313 39
Cuts at: 8073 8800 9011 9481 9846 9939 9945 566
Size: 727 211 470 365 93 6 3628
Fragments arranged by size:
3628 1795 1654 1241 727 488 470 408
365 351 313 218 211 201 189 172
156 121 93 86 75 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1446 2043 2086 4878 7285 9649 10183 11062 11776
Size: 597 43 2792 2407 2364 534 879 714
Cuts at: 11776 12074 1446
Size: 298 2379
Fragments arranged by size:
2792 2407 2379 2364 879 714 597 534
298 43
BsaI GGTCTCn'nnnn_
Cuts at: 667 4612 7466 8104 12711 667
Size: 3945 2854 638 4607 963
Fragments arranged by size:
4607 3945 2854 963 638
BsaAI yAC'GTr
Cuts at: 4806 4953 6757 6771 7515 8610 10360 4806
Size: 147 1804 14 744 1095 1750 7453
Fragments arranged by size:
7453 1804 1750 1095 744 147 14
BsaBI GATnn'nnATC
Cuts at: 6 6907 10521 12558 6
Size: 6901 3614 2037 455
Fragments arranged by size:
6901 3614 2037 455
BsaHI Gr'CG_yC
Cuts at: 627 1652 3522 4493 5030 5044 5171 5792 10438
Size: 1025 1870 971 537 14 127 621 4646
Cuts at: 10438 10686 11388 12884 627
Size: 248 702 1496 750
Fragments arranged by size:
4646 1870 1496 1025 971 750 702 621
537 248 127 14
BsaWI w'CCGG_w
Cuts at: 992 1978 10196 10717 11395 11806 11953 12227 992
Size: 986 8218 521 678 411 147 274 1772
Fragments arranged by size:
8218 1772 986 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 312 342 1654 1684 3921 3951 4966 4996 5486
Size: 30 1312 30 2237 30 1015 30 490
Cuts at: 5486 5516 6114 6144 7082 7112 8316 8346 8936
Size: 30 598 30 938 30 1204 30 590
Cuts at: 8936 8966 312
Size: 30 4353
Fragments arranged by size:
4353 2237 1312 1204 1015 938 598 590
490 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 320 1420 1776 1933 2144 3995 5266 5285
Size: 40 1100 356 157 211 1851 1271 19
Cuts at: 5285 6569 9270 9448 9583 9586 9875 280
Size: 1284 2701 178 135 3 289 3412
Fragments arranged by size:
3412 2701 1851 1284 1271 1100 356 289
211 178 157 135 40 19 3
BseYI C'CCAG_C
Cuts at: 120 204 211 238 622 1134 1880 2724 2911
Size: 84 7 27 384 512 746 844 187
Cuts at: 2911 4092 5824 5875 5917 6028 6746 6870 7726
Size: 1181 1732 51 42 111 718 124 856
Cuts at: 7726 8217 8311 8350 8476 9130 9444 9513 9690
Size: 491 94 39 126 654 314 69 177
Cuts at: 9690 9862 10432 11190 11855 12263 120
Size: 172 570 758 665 408 864
Fragments arranged by size:
1732 1181 864 856 844 758 746 718
665 654 570 512 491 408 384 314
187 177 172 126 124 111 94 84
69 51 42 39 27 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1232 1471 2395 2777 3193 3670 3676 3889 4054
Size: 239 924 382 416 477 6 213 165
Cuts at: 4054 4393 4405 4706 4786 4823 4873 5918 5930
Size: 339 12 301 80 37 50 1045 12
Cuts at: 5930 7537 8632 9431 9473 9979 1232
Size: 1607 1095 799 42 506 4260
Fragments arranged by size:
4260 1607 1095 1045 924 799 506 477
416 382 339 301 239 213 165 80
50 42 37 12 12 6
BsiEI CG_ry'CG
Cuts at: 104 4659 4924 10549 10595 11825 104
Size: 4555 265 5625 46 1230 1286
Fragments arranged by size:
5625 4555 1286 1230 265 46
BsiHKAI G_wGCw'C
Cuts at: 2026 3987 4200 4952 6956 7271 7514 8074 8163
Size: 1961 213 752 2004 315 243 560 89
Cuts at: 8163 8438 9110 9578 10021 10799 10989 11849 2026
Size: 275 672 468 443 778 190 860 3184
Fragments arranged by size:
3184 2004 1961 860 778 752 672 560
468 443 315 275 243 213 190 89
BsmI GAATG_Cn'
Cuts at: 6351 6422 6351
Size: 71 12936
BsmBI CGTCTCn'nnnn_
Cuts at: 5178 7305 5178
Size: 2127 10880
Bsp1286I G_dGCh'C
Cuts at: 157 376 819 1125 1141 1550 1835 1879 2026
Size: 219 443 306 16 409 285 44 147
Cuts at: 2026 2057 2196 2235 2430 2723 2862 2981 3031
Size: 31 139 39 195 293 139 119 50
Cuts at: 3031 3199 3673 3987 4165 4184 4200 4240 4460
Size: 168 474 314 178 19 16 40 220
Cuts at: 4460 4793 4952 5232 5290 5370 5825 5924 6674
Size: 333 159 280 58 80 455 99 750
Cuts at: 6674 6871 6886 6956 6985 7061 7115 7271 7485
Size: 197 15 70 29 76 54 156 214
Cuts at: 7485 7514 7758 8054 8074 8163 8423 8438 8576
Size: 29 244 296 20 89 260 15 138
Cuts at: 8576 8586 8709 8736 8997 9110 9443 9487 9578
Size: 10 123 27 261 113 333 44 91
Cuts at: 9578 9619 9758 10021 10632 10725 10799 10989 11051
Size: 41 139 263 611 93 74 190 62
Cuts at: 11051 11849 157
Size: 798 1315
Fragments arranged by size:
1315 798 750 611 474 455 443 409
333 333 314 306 296 293 285 280
263 261 260 244 220 219 214 197
195 190 178 168 159 156 147 139
139 139 138 123 119 113 99 93
91 89 80 76 74 70 62 58
54 50 44 44 41 40 39 31
29 29 27 20 19 16 16 15
15 10
BspEI T'CCGG_A
Cuts at: 1978 1978
Size: 13007
BspHI T'CATG_A
Cuts at: 3987 5745 7173 11439 3987
Size: 1758 1428 4266 5555
Fragments arranged by size:
5555 4266 1758 1428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 549 924 1524 1893 3140 3642 4026 4365 4995
Size: 375 600 369 1247 502 384 339 630
Cuts at: 4995 6672 7236 7598 8723 8918 9366 9529 9611
Size: 1677 564 362 1125 195 448 163 82
Cuts at: 9611 10057 10271 10573 10954 549
Size: 446 214 302 381 2602
Fragments arranged by size:
2602 1677 1247 1125 630 600 564 502
448 446 384 381 375 369 362 339
302 214 195 163 82
BsrBI CCG'CTC
Cuts at: 50 761 1059 3957 4086 6179 9815 11299 11353
Size: 711 298 2898 129 2093 3636 1484 54
Cuts at: 11353 12837 13002 50
Size: 1484 165 55
Fragments arranged by size:
3636 2898 2093 1484 1484 711 298 165
129 55 54
BsrDI GCAATG_nn'
Cuts at: 178 10919 178
Size: 10741 2266
BsrFI r'CCGG_y
Cuts at: 4557 4658 5794 6110 6750 9562 11005 11186 4557
Size: 101 1136 316 640 2812 1443 181 6378
Fragments arranged by size:
6378 2812 1443 1136 640 316 181 101
BsrGI T'GTAC_A
Cuts at: 3308 3866 4472 3308
Size: 558 606 11843
Fragments arranged by size:
11843 606 558
BssHII G'CGCG_C
Cuts at: 4314 4316 4355 4417 4576 4690 4702 4314
Size: 2 39 62 159 114 12 12619
Fragments arranged by size:
12619 159 114 62 39 12 2
BssSI C'ACGA_G
Cuts at: 608 1109 7469 7499 8014 8278 11278 11986 608
Size: 501 6360 30 515 264 3000 708 1629
Fragments arranged by size:
6360 3000 1629 708 515 501 264 30
BstAPI GCAn_nnn'nTGC
Cuts at: 949 8167 9220 10384 949
Size: 7218 1053 1164 3572
Fragments arranged by size:
7218 3572 1164 1053
BstBI TT'CG_AA
Cuts at: 4295 11368 4295
Size: 7073 5934
BstXI CCAn_nnnn'nTGG
Cuts at: 227 2370 3441 4786 227
Size: 2143 1071 1345 8448
Fragments arranged by size:
8448 2143 1345 1071
BstYI r'GATC_y
Cuts at: 1 1602 1981 2251 2601 3429 3834 4074 4824
Size: 1601 379 270 350 828 405 240 750
Cuts at: 4824 5196 6821 7065 9236 10032 10499 10522 10857
Size: 372 1625 244 2171 796 467 23 335
Cuts at: 10857 11103 11409 11421 11507 12559 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2171 1625 1601 1052 828 796 750 467
449 405 379 372 350 335 306 270
246 244 240 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1556 1686 1871 2982 3138 4260 5390 7943 8363
Size: 130 185 1111 156 1122 1130 2553 420
Cuts at: 8363 1556
Size: 6200
Fragments arranged by size:
6200 2553 1130 1122 1111 420 185 156
130
BtgI C'CryG_G
Cuts at: 2108 2167 2256 2933 3087 4184 4309 4793 4913
Size: 59 89 677 154 1097 125 484 120
Cuts at: 4913 4977 6520 7476 7836 8791 8864 8927 9168
Size: 64 1543 956 360 955 73 63 241
Cuts at: 9168 9294 9579 9728 2108
Size: 126 285 149 5387
Fragments arranged by size:
5387 1543 1097 956 955 677 484 360
285 241 154 149 126 125 120 89
73 64 63 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5975 10957 11138 11301 5975
Size: 4982 181 163 7681
Fragments arranged by size:
7681 4982 181 163
BtsI GCAGTG_nn'
Cuts at: 458 838 1459 1864 2718 2825 2851 3146 3335
Size: 380 621 405 854 107 26 295 189
Cuts at: 3335 3713 3929 5906 6330 7135 7290 7454 9061
Size: 378 216 1977 424 805 155 164 1607
Cuts at: 9061 9449 9722 10250 458
Size: 388 273 528 3215
Fragments arranged by size:
3215 1977 1607 854 805 621 528 424
405 388 380 378 295 273 216 189
164 155 107 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 6216 6251 6216
Size: 35 12972
DraI TTT'AAA
Cuts at: 10006 10006
Size: 13007
DraIII CAC_nnn'GTG
Cuts at: 3259 3969 3982 3259
Size: 710 13 12284
Fragments arranged by size:
12284 710 13
DrdI GACnn_nn'nnGTC
Cuts at: 10713 12057 10713
Size: 1344 11663
EaeI y'GGCC_r
Cuts at: 383 1060 1754 2351 4058 4875 4921 4997 5539
Size: 677 694 597 1707 817 46 76 542
Cuts at: 5539 5769 6056 6224 6237 6851 7080 8252 8592
Size: 230 287 168 13 614 229 1172 340
Cuts at: 8592 9201 9266 9560 9635 9679 10592 10766 11157
Size: 609 65 294 75 44 913 174 391
Cuts at: 11157 11184 12529 383
Size: 27 1345 861
Fragments arranged by size:
1707 1345 1172 913 861 817 694 677
614 609 597 542 391 340 294 287
230 229 174 168 76 75 65 46
44 27 13
EagI C'GGCC_G
Cuts at: 4921 10592 4921
Size: 5671 7336
EarI CTCTTCn'nnn_
Cuts at: 145 2620 5228 6220 8676 9131 11030 11240 145
Size: 2475 2608 992 2456 455 1899 210 1912
Fragments arranged by size:
2608 2475 2456 1912 1899 992 455 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6706 11215 11957 12103 12817 6706
Size: 4509 742 146 714 6896
Fragments arranged by size:
6896 4509 742 714 146
EcoICRI GAG'CTC
Cuts at: 6954 8072 9576 10019 6954
Size: 1118 1504 443 9942
Fragments arranged by size:
9942 1504 1118 443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 619 689 698 706 802 2401 2716 2819
Size: 107 70 9 8 96 1599 315 103
Cuts at: 2819 3025 3031 3268 3337 3572 3608 3734 3785
Size: 206 6 237 69 235 36 126 51
Cuts at: 3785 4006 4922 5401 5540 5648 5780 5839 5852
Size: 221 916 479 139 108 132 59 13
Cuts at: 5852 6018 6092 6734 7056 7356 7469 7604 7639
Size: 166 74 642 322 300 113 135 35
Cuts at: 7639 8252 8540 8827 9173 9991 10832 11264 11617
Size: 613 288 287 346 818 841 432 353
Cuts at: 11617 12279 512
Size: 662 1240
Fragments arranged by size:
1599 1240 916 841 818 662 642 613
479 432 353 346 322 315 300 288
287 237 235 221 206 166 139 135
132 126 113 108 107 103 96 74
70 69 59 51 36 35 13 9
8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1555 1610 3649 3914 4771 6467 6679 7249 8344
Size: 55 2039 265 857 1696 212 570 1095
Cuts at: 8344 9305 1555
Size: 961 5257
Fragments arranged by size:
5257 2039 1696 1095 961 857 570 265
212 55
EcoO109I rG'GnC_Cy
Cuts at: 161 815 822 1973 2053 2054 2977 2978 3162
Size: 654 7 1151 80 1 923 1 184
Cuts at: 3162 3195 3196 3511 3768 4089 4818 5007 5229
Size: 33 1 315 257 321 729 189 222
Cuts at: 5229 5400 5484 5720 5921 6025 6196 6670 6671
Size: 171 84 236 201 104 171 474 1
Cuts at: 6671 7024 7058 7111 7112 7394 7747 8359 8824
Size: 353 34 53 1 282 353 612 465
Cuts at: 8824 9157 9439 9440 9934 10146 161
Size: 333 282 1 494 212 3022
Fragments arranged by size:
3022 1151 923 729 654 612 494 474
465 353 353 333 321 315 282 282
257 236 222 212 201 189 184 171
171 104 84 80 53 34 33 7
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 10011 10011
Size: 13007
EcoRV GAT'ATC
Cuts at: 8871 8871
Size: 13007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 11026 11026
Size: 13007
FspI TGC'GCA
Cuts at: 5842 10788 5842
Size: 4946 8061
HaeII r_GCGC'y
Cuts at: 1655 4177 4269 4435 4441 4471 4564 4591 4598
Size: 2522 92 166 6 30 93 27 7
Cuts at: 4598 4909 5047 5795 10441 10689 11919 12690 1655
Size: 311 138 748 4646 248 1230 771 1972
Fragments arranged by size:
4646 2522 1972 1230 771 748 311 248
166 138 93 92 30 27 7 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1467 1669 1701 2236 2268 2305 2337 2423
Size: 32 202 32 535 32 37 32 86
Cuts at: 2423 2455 2617 2649 2857 2889 3013 3045 3121
Size: 32 162 32 208 32 124 32 76
Cuts at: 3121 3153 3490 3522 3621 3653 4243 4275 4966
Size: 32 337 32 99 32 590 32 691
Cuts at: 4966 4998 5272 5304 5355 5387 5486 5518 6194
Size: 32 274 32 51 32 99 32 676
Cuts at: 6194 6226 6694 6726 7003 7035 7082 7114 7303
Size: 32 468 32 277 32 47 32 189
Cuts at: 7303 7335 8056 8088 8103 8135 10849 10879 10881
Size: 32 721 32 15 32 2714 30 2
Cuts at: 10881 10911 1435
Size: 30 3531
Fragments arranged by size:
3531 2714 721 691 676 590 535 468
337 277 274 208 202 189 162 124
99 99 86 76 51 47 37 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
15 2
HincII GTy'rAC
Cuts at: 1503 2333 2883 5034 5303 7380 10046 1503
Size: 830 550 2151 269 2077 2666 4464
Fragments arranged by size:
4464 2666 2151 2077 830 550 269
HindIII A'AGCT_T
Cuts at: 2061 5184 8445 2061
Size: 3123 3261 6623
Fragments arranged by size:
6623 3261 3123
HpaI GTT'AAC
Cuts at: 2883 5303 2883
Size: 2420 10587
KasI G'GCGC_C
Cuts at: 1651 5043 5791 10437 10685 1651
Size: 3392 748 4646 248 3973
Fragments arranged by size:
4646 3973 3392 748 248
KpnI G_GTAC'C
Cuts at: 565 3787 10027 565
Size: 3222 6240 3545
Fragments arranged by size:
6240 3545 3222
MfeI C'AATT_G
Cuts at: 8186 8186
Size: 13007
MluI A'CGCG_T
Cuts at: 4758 4842 12440 4758
Size: 84 7598 5325
Fragments arranged by size:
7598 5325 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2669 4469 4907 6255 7938 8324 9651 11766
Size: 963 1800 438 1348 1683 386 1327 2115
Cuts at: 11766 11950 1706
Size: 184 2763
Fragments arranged by size:
2763 2115 1800 1683 1348 1327 963 438
386 184
MscI TGG'CCA
Cuts at: 385 1756 2353 5771 6058 6226 6853 8594 9268
Size: 1371 597 3418 287 168 627 1741 674
Cuts at: 9268 9637 10768 385
Size: 369 1131 2624
Fragments arranged by size:
3418 2624 1741 1371 1131 674 627 597
369 287 168
MslI CAynn'nnrTG
Cuts at: 1273 1310 2012 2215 2530 4213 5935 5953 6142
Size: 37 702 203 315 1683 1722 18 189
Cuts at: 6142 6262 7408 7568 8248 8278 9197 9727 10996
Size: 120 1146 160 680 30 919 530 1269
Cuts at: 10996 11123 1273
Size: 127 3157
Fragments arranged by size:
3157 1722 1683 1269 1146 919 702 680
530 315 203 189 160 127 120 37
30 18
MspA1I CmG'CkG
Cuts at: 277 365 441 780 1080 1743 2083 2215 2379
Size: 88 76 339 300 663 340 132 164
Cuts at: 2379 3546 4026 4096 4132 4186 4428 4795 5574
Size: 1167 480 70 36 54 242 367 779
Cuts at: 5574 5848 5914 7146 7152 7521 7657 7704 7831
Size: 274 66 1232 6 369 136 47 127
Cuts at: 7831 8744 9778 9850 10118 10432 10792 11576 11821
Size: 913 1034 72 268 314 360 784 245
Cuts at: 11821 277
Size: 1463
Fragments arranged by size:
1463 1232 1167 1034 913 784 779 663
480 369 367 360 340 339 314 300
274 268 245 242 164 136 132 127
88 76 72 70 66 54 47 36
6
NaeI GCC'GGC
Cuts at: 4559 6752 9564 11188 4559
Size: 2193 2812 1624 6378
Fragments arranged by size:
6378 2812 2193 1624
NarI GG'CG_CC
Cuts at: 1652 5044 5792 10438 10686 1652
Size: 3392 748 4646 248 3973
Fragments arranged by size:
4646 3973 3392 748 248
NcoI C'CATG_G
Cuts at: 2108 2256 2108
Size: 148 12859
NgoMIV G'CCGG_C
Cuts at: 4557 6750 9562 11186 4557
Size: 2193 2812 1624 6378
Fragments arranged by size:
6378 2812 2193 1624
NheI G'CTAG_C
Cuts at: 6319 6319
Size: 13007
NspI r_CATG'y
Cuts at: 1162 3703 4405 5311 7978 8296 10060 10560 12163
Size: 2541 702 906 2667 318 1764 500 1603
Cuts at: 12163 1162
Size: 2006
Fragments arranged by size:
2667 2541 2006 1764 1603 906 702 500
318
PasI CC'CwG_GG
Cuts at: 639 2833 3466 6355 6377 6947 9930 639
Size: 2194 633 2889 22 570 2983 3716
Fragments arranged by size:
3716 2983 2889 2194 633 570 22
PciI A'CATG_T
Cuts at: 12159 12159
Size: 13007
PflMI CCAn_nnn'nTGG
Cuts at: 894 1095 1291 2280 3003 3259 3612 4029 5359
Size: 201 196 989 723 256 353 417 1330
Cuts at: 5359 6133 6636 8100 8601 9151 9520 9633 894
Size: 774 503 1464 501 550 369 113 4268
Fragments arranged by size:
4268 1464 1330 989 774 723 550 503
501 417 369 353 256 201 196 113
PfoI T'CCnGG_A
Cuts at: 3801 6809 8195 8839 3801
Size: 3008 1386 644 7969
Fragments arranged by size:
7969 3008 1386 644
PmeI GTTT'AAAC
Cuts at: 10006 10006
Size: 13007
PmlI CAC'GTG
Cuts at: 4806 6757 7515 8610 4806
Size: 1951 758 1095 9203
Fragments arranged by size:
9203 1951 1095 758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11453 11485 11453
Size: 32 12975
PpuMI rG'GwC_Cy
Cuts at: 1973 3162 3511 4089 4818 5007 5400 5484 5720
Size: 1189 349 578 729 189 393 84 236
Cuts at: 5720 7024 7394 8359 9157 9934 1973
Size: 1304 370 965 798 777 5046
Fragments arranged by size:
5046 1304 1189 965 798 777 729 578
393 370 349 236 189 84
PshAI GACnn'nnGTC
Cuts at: 1971 1971
Size: 13007
PspOMI G'GGCC_C
Cuts at: 815 2053 2977 3195 4789 5228 5920 6670 7057
Size: 1238 924 218 1594 439 692 750 387
Cuts at: 7057 7111 8993 9439 815
Size: 54 1882 446 4383
Fragments arranged by size:
4383 1882 1594 1238 924 750 692 446
439 387 218 54
PstI C_TGCA'G
Cuts at: 465 560 1052 1904 3746 3827 3936 5718 5735
Size: 95 492 852 1842 81 109 1782 17
Cuts at: 5735 5853 6284 6542 6683 7151 7526 7830 9068
Size: 118 431 258 141 468 375 304 1238
Cuts at: 9068 9225 9783 10054 10739 465
Size: 157 558 271 685 2733
Fragments arranged by size:
2733 1842 1782 1238 852 685 558 492
468 431 375 304 271 258 157 141
118 109 95 81 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 13007
PvuII CAG'CTG
Cuts at: 780 1743 2083 2215 2379 3546 4026 4132 5574
Size: 963 340 132 164 1167 480 106 1442
Cuts at: 5574 5848 5914 7146 7152 7521 7704 7831 9778
Size: 274 66 1232 6 369 183 127 1947
Cuts at: 9778 9850 10432 10792 780
Size: 72 582 360 2995
Fragments arranged by size:
2995 1947 1442 1232 1167 963 582 480
369 360 340 274 183 164 132 127
106 72 66 6
RsrII CG'GwC_CG
Cuts at: 11202 11202
Size: 13007
SacI G_AGCT'C
Cuts at: 6956 8074 9578 10021 6956
Size: 1118 1504 443 9942
Fragments arranged by size:
9942 1504 1118 443
SacII CC_GC'GG
Cuts at: 4187 4796 4187
Size: 609 12398
SalI G'TCGA_C
Cuts at: 10044 10044
Size: 13007
SanDI GG'GwC_CC
Cuts at: 4089 4818 5484 5720 7394 9934 4089
Size: 729 666 236 1674 2540 7162
Fragments arranged by size:
7162 2540 1674 729 666 236
SapI GCTCTTCn'nnn_
Cuts at: 145 11030 11240 145
Size: 10885 210 1912
Fragments arranged by size:
10885 1912 210
SbfI CC_TGCA'GG
Cuts at: 560 1904 3746 5718 10054 560
Size: 1344 1842 1972 4336 3513
Fragments arranged by size:
4336 3513 1972 1842 1344
ScaI AGT'ACT
Cuts at: 12524 12524
Size: 13007
SexAI A'CCwGG_T
Cuts at: 2559 7250 9370 2559
Size: 4691 2120 6196
Fragments arranged by size:
6196 4691 2120
SfcI C'TryA_G
Cuts at: 33 461 556 775 1048 1900 2410 2485 2735
Size: 428 95 219 273 852 510 75 250
Cuts at: 2735 3531 3742 3823 3932 5714 5731 5737 5849
Size: 796 211 81 109 1782 17 6 112
Cuts at: 5849 6185 6280 6417 6538 6679 6729 7147 7522
Size: 336 95 137 121 141 50 418 375
Cuts at: 7522 7625 7665 7826 8699 9064 9221 9426 9779
Size: 103 40 161 873 365 157 205 353
Cuts at: 9779 10050 10735 11703 11894 33
Size: 271 685 968 191 1146
Fragments arranged by size:
1782 1146 968 873 852 796 685 510
428 418 375 365 353 336 273 271
250 219 211 205 191 161 157 141
137 121 112 109 103 95 95 81
75 50 40 17 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 1937 2359 2976 8009 9274 1937
Size: 422 617 5033 1265 5670
Fragments arranged by size:
5670 5033 1265 617 422
SfoI GGC'GCC
Cuts at: 1653 5045 5793 10439 10687 1653
Size: 3392 748 4646 248 3973
Fragments arranged by size:
4646 3973 3392 748 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 13007
SmaI CCC'GGG
Cuts at: 3019 4816 5590 6316 7753 9787 10029 3019
Size: 1797 774 726 1437 2034 242 5997
Fragments arranged by size:
5997 2034 1797 1437 774 726 242
SmlI C'TyrA_G
Cuts at: 1425 2058 2101 4893 7264 9664 10162 11077 11791
Size: 633 43 2792 2371 2400 498 915 714
Cuts at: 11791 12053 1425
Size: 262 2379
Fragments arranged by size:
2792 2400 2379 2371 915 714 633 498
262 43
SpeI A'CTAG_T
Cuts at: 67 67
Size: 13007
SphI G_CATG'C
Cuts at: 4405 8296 10060 10560 4405
Size: 3891 1764 500 6852
Fragments arranged by size:
6852 3891 1764 500
SrfI GCCC'GGGC
Cuts at: 9787 9787
Size: 13007
StuI AGG'CCT
Cuts at: 787 2099 2970 3565 7984 787
Size: 1312 871 595 4419 5810
Fragments arranged by size:
5810 4419 1312 871 595
StyI C'CwwG_G
Cuts at: 1611 1955 2108 2256 2309 2348 3993 5855 6662
Size: 344 153 148 53 39 1645 1862 807
Cuts at: 6662 7424 8939 8979 9179 10141 10495 1611
Size: 762 1515 40 200 962 354 4123
Fragments arranged by size:
4123 1862 1645 1515 962 807 762 354
344 200 153 148 53 40 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 764 1369 1675 1894 1948 2484 3251 3261 3515
Size: 605 306 219 54 536 767 10 254
Cuts at: 3515 4183 4628 5994 8452 8890 9507 9752 10590
Size: 668 445 1366 2458 438 617 245 838
Cuts at: 10590 10723 11182 11391 764
Size: 133 459 209 2380
Fragments arranged by size:
2458 2380 1366 838 767 668 617 605
536 459 445 438 306 254 245 219
209 133 54 10
TatI w'GTAC_w
Cuts at: 338 3308 3866 4472 5066 6466 7808 7881 8136
Size: 2970 558 606 594 1400 1342 73 255
Cuts at: 8136 12522 338
Size: 4386 823
Fragments arranged by size:
4386 2970 1400 1342 823 606 594 558
255 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 312 344 1654 1686 3797 3829 3921 3953 5969
Size: 32 1310 32 2111 32 92 32 2016
Cuts at: 5969 6001 6114 6146 7339 7371 8543 8575 8936
Size: 32 113 32 1193 32 1172 32 361
Cuts at: 8936 8968 12524 12556 312
Size: 32 3556 32 763
Fragments arranged by size:
3556 2111 2016 1310 1193 1172 763 361
113 92 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1363 10804 1363
Size: 9441 3566
XbaI T'CTAG_A
Cuts at: 7785 10038 7785
Size: 2253 10754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1618 1800 2254 2281 2430 3187 3260 3348 3583
Size: 182 454 27 149 757 73 88 235
Cuts at: 3583 6754 6807 9462 10502 1618
Size: 3171 53 2655 1040 4123
Fragments arranged by size:
4123 3171 2655 1040 757 454 235 182
149 88 73 53 27
XmaI C'CCGG_G
Cuts at: 3017 4814 5588 6314 7751 9785 10027 3017
Size: 1797 774 726 1437 2034 242 5997
Fragments arranged by size:
5997 2034 1797 1437 774 726 242
ZraI GAC'GTC
Cuts at: 3523 5031 3523
Size: 1508 11499
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI BfrBI BsiWI BstEII
BstZ17I ClaI FseI FspAI NdeI NotI NruI NsiI
PacI PsiI PspXI PsrI SgrAI SnaBI SspI SwaI
XhoI XmnI