Restriction map for
pF1KA1775
(Circular) (Six-base) MAPSORT of: pF1KA1775.seq Check: 1048 from: 1 to: 5690
pF1KA1775 5690 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 269 2728 269
Size: 2459 3231
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 2088 3515 3947 4300 4962 925
Size: 1163 1427 432 353 662 1653
Fragments arranged by size:
1653 1427 1163 662 432 353
AflIII A'CryG_T
Cuts at: 1961 3042 4842 5123 1961
Size: 1081 1800 281 2528
Fragments arranged by size:
2528 1800 1081 281
AleI CACnn'nnGTG
Cuts at: 668 1594 2242 668
Size: 926 648 4116
Fragments arranged by size:
4116 926 648
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 657 1829 1861 625
Size: 32 1172 32 4454
Fragments arranged by size:
4454 1172 32 32
AlwNI CAG_nnn'CTG
Cuts at: 292 604 636 1494 2143 2995 4433 292
Size: 312 32 858 649 852 1438 1549
Fragments arranged by size:
1549 1438 858 852 649 312 32
ApaLI G'TGCA_C
Cuts at: 2665 4528 2665
Size: 1863 3827
ApoI r'AATT_y
Cuts at: 947 1288 2004 2694 5043 5299 947
Size: 341 716 690 2349 256 1338
Fragments arranged by size:
2349 1338 716 690 341 256
AseI AT'TA_AT
Cuts at: 20 992 20
Size: 972 4718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 541 2472 2710 541
Size: 1931 238 3521
Fragments arranged by size:
3521 1931 238
BamHI G'GATC_C
Cuts at: 1599 2715 1599
Size: 1116 4574
BanI G'GyrC_C
Cuts at: 501 2227 2508 2706 3120 3368 3403 501
Size: 1726 281 198 414 248 35 2788
Fragments arranged by size:
2788 1726 414 281 248 198 35
BanII G_rGCy'C
Cuts at: 895 1030 1087 1099 1573 1955 2095 2164 2481
Size: 135 57 12 474 382 140 69 317
Cuts at: 2481 2639 2704 3734 895
Size: 158 65 1030 2851
Fragments arranged by size:
2851 1030 474 382 317 158 140 135
69 65 57 12
BbeI G_GCGC'C
Cuts at: 505 2512 3124 3372 505
Size: 2007 612 248 2823
Fragments arranged by size:
2823 2007 612 248
BbsI GAAGACnn'nnnn_
Cuts at: 834 1861 834
Size: 1027 4663
BbvCI CC'TCA_GC
Cuts at: 1721 1787 1721
Size: 66 5624
BciVI GTATCCnnnnn_n'
Cuts at: 3588 4644 5690 3588
Size: 1056 1046 3588
Fragments arranged by size:
3588 1056 1046
BclI T'GATC_A
Cuts at: 3210 3210
Size: 5690
BglI GCCn_nnn'nGGC
Cuts at: 133 2316 133
Size: 2183 3507
BglII A'GATC_T
Cuts at: 1991 3205 5242 1991
Size: 1214 2037 2439
Fragments arranged by size:
2439 2037 1214
BlpI GC'TnA_GC
Cuts at: 2802 2802
Size: 5690
Bme1580I G_kGCm'C
Cuts at: 853 2669 3315 3408 4532 853
Size: 1816 646 93 1124 2011
Fragments arranged by size:
2011 1816 1124 646 93
BmrI ACTGGGnnnn_n'
Cuts at: 1114 1658 1691 1870 2523 2902 2983 3318 5462
Size: 544 33 179 653 379 81 335 2144
Cuts at: 5462 1114
Size: 1342
Fragments arranged by size:
2144 1342 653 544 379 335 179 81
33
BmtI G_CTAG'C
Cuts at: 1712 1712
Size: 5690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2169 4057 2169
Size: 1888 3802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 667 1024 1025 1557 626
Size: 41 357 1 532 4759
Fragments arranged by size:
4759 532 357 41 1
Bpu10I CC'TnA_GC
Cuts at: 164 1721 1787 164
Size: 1557 66 4067
Fragments arranged by size:
4067 1557 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 1984 2107 2359 2623 2866 3745 4459 4757
Size: 753 123 252 264 243 879 714 298
Cuts at: 4757 1231
Size: 2164
Fragments arranged by size:
2164 879 753 714 298 264 252 243
123
BsaI GGTCTCn'nnnn_
Cuts at: 293 920 2140 5394 293
Size: 627 1220 3254 589
Fragments arranged by size:
3254 1220 627 589
BsaAI yAC'GTr
Cuts at: 267 3043 267
Size: 2776 2914
BsaBI GATnn'nnATC
Cuts at: 6 2131 3204 5241 6
Size: 2125 1073 2037 455
Fragments arranged by size:
2125 2037 1073 455
BsaHI Gr'CG_yC
Cuts at: 502 2509 3121 3369 4071 5567 502
Size: 2007 612 248 702 1496 625
Fragments arranged by size:
2007 1496 702 625 612 248
BsaWI w'CCGG_w
Cuts at: 2879 3400 4078 4489 4636 4910 2879
Size: 521 678 411 147 274 3659
Fragments arranged by size:
3659 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 599 625 629 655 1759 1789 2077
Size: 30 277 26 4 26 1104 30 288
Cuts at: 2077 2107 292
Size: 30 3875
Fragments arranged by size:
3875 1104 288 277 30 30 30 26
26 4
BseYI C'CCAG_C
Cuts at: 331 556 688 1034 1098 1697 2338 2500 2512
Size: 225 132 346 64 599 641 162 12
Cuts at: 2512 3115 3873 4538 4946 331
Size: 603 758 665 408 1075
Fragments arranged by size:
1075 758 665 641 603 599 408 346
225 162 132 64 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 2014 2095 622
Size: 1392 81 4217
Fragments arranged by size:
4217 1392 81
BsiEI CG_ry'CG
Cuts at: 104 3232 3278 4508 104
Size: 3128 46 1230 1286
Fragments arranged by size:
3128 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1217 2327 2389 2481 2639 2669 2704 3482 3672
Size: 1110 62 92 158 30 35 778 190
Cuts at: 3672 4532 1217
Size: 860 2375
Fragments arranged by size:
2375 1110 860 778 190 158 92 62
35 30
BsmBI CGTCTCn'nnnn_
Cuts at: 2553 2553
Size: 5690
Bsp1286I G_dGCh'C
Cuts at: 853 895 1030 1087 1099 1217 1573 1955 2095
Size: 42 135 57 12 118 356 382 140
Cuts at: 2095 2164 2327 2389 2481 2639 2669 2704 3315
Size: 69 163 62 92 158 30 35 611
Cuts at: 3315 3408 3482 3672 3734 4532 853
Size: 93 74 190 62 798 2011
Fragments arranged by size:
2011 798 611 382 356 190 163 158
140 135 118 93 92 74 69 62
62 57 42 35 30 12
BspHI T'CATG_A
Cuts at: 4122 4122
Size: 5690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 561 644 2740 2954 3256 3637 561
Size: 83 2096 214 302 381 2614
Fragments arranged by size:
2614 2096 381 302 214 83
BsrBI CCG'CTC
Cuts at: 50 1178 2015 3982 4036 5520 5685 50
Size: 1128 837 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 1128 837 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3602 3602
Size: 5690
BsrFI r'CCGG_y
Cuts at: 119 2224 3688 3869 119
Size: 2105 1464 181 1940
Fragments arranged by size:
2105 1940 1464 181
BsrGI T'GTAC_A
Cuts at: 1065 1065
Size: 5690
BssSI C'ACGA_G
Cuts at: 3961 4669 3961
Size: 708 4982
BstAPI GCAn_nnn'nTGC
Cuts at: 636 3067 636
Size: 2431 3259
BstBI TT'CG_AA
Cuts at: 4051 4051
Size: 5690
BstEII G'GTnAC_C
Cuts at: 480 480
Size: 5690
BstXI CCAn_nnnn'nTGG
Cuts at: 1581 1923 2244 2601 1581
Size: 342 321 357 4670
Fragments arranged by size:
4670 357 342 321
BstYI r'GATC_y
Cuts at: 1 1232 1599 1991 2715 3182 3205 3540 3786
Size: 1231 367 392 724 467 23 335 246
Cuts at: 3786 4092 4104 4190 5242 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1231 1052 724 467 449 392 367 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 270 270
Size: 5690
Bsu36I CC'TnA_GG
Cuts at: 293 293
Size: 5690
BtgI C'CryG_G
Cuts at: 653 734 797 917 2165 2210 2231 653
Size: 81 63 120 1248 45 21 4112
Fragments arranged by size:
4112 1248 120 81 63 45 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3640 3821 3984 3640
Size: 181 163 5346
Fragments arranged by size:
5346 181 163
BtsI GCAGTG_nn'
Cuts at: 1727 1801 2933 1727
Size: 74 1132 4484
Fragments arranged by size:
4484 1132 74
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1355 1390 1355
Size: 35 5655
DraI TTT'AAA
Cuts at: 2689 2689
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 199 3396 4740 199
Size: 3197 1344 1149
Fragments arranged by size:
3197 1344 1149
EaeI y'GGCC_r
Cuts at: 458 2222 2234 2246 3275 3449 3840 3867 5212
Size: 1764 12 12 1029 174 391 27 1345
Cuts at: 5212 458
Size: 936
Fragments arranged by size:
1764 1345 1029 936 391 174 27 12
12
EagI C'GGCC_G
Cuts at: 3275 3275
Size: 5690
EarI CTCTTCn'nnn_
Cuts at: 383 1666 2653 3713 3923 383
Size: 1283 987 1060 210 2150
Fragments arranged by size:
2150 1283 1060 987 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3898 4640 4786 5500 3898
Size: 742 146 714 4088
Fragments arranged by size:
4088 742 714 146
EcoICRI GAG'CTC
Cuts at: 2479 2637 2702 2479
Size: 158 65 5467
Fragments arranged by size:
5467 158 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 667 925 1024 1025 1557 2088 3515 3947
Size: 41 258 99 1 532 531 1427 432
Cuts at: 3947 4300 4962 626
Size: 353 662 1354
Fragments arranged by size:
1427 1354 662 532 531 432 353 258
99 41 1
EcoNI CCTnn'n_nnAGG
Cuts at: 241 241
Size: 5690
EcoO109I rG'GnC_Cy
Cuts at: 713 1242 1309 1612 1871 2304 2829 713
Size: 529 67 303 259 433 525 3574
Fragments arranged by size:
3574 529 525 433 303 259 67
EcoRI G'AATT_C
Cuts at: 947 2004 2694 947
Size: 1057 690 3943
Fragments arranged by size:
3943 1057 690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2697 3709 2697
Size: 1012 4678
FspI TGC'GCA
Cuts at: 3471 3471
Size: 5690
HaeII r_GCGC'y
Cuts at: 117 505 2512 3124 3372 4602 5373 117
Size: 388 2007 612 248 1230 771 434
Fragments arranged by size:
2007 1230 771 612 434 388 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 290 292 322 324 586 599 618 631 2077
Size: 2 30 2 262 13 19 13 1446
Cuts at: 2077 2109 3532 3562 3564 3594 290
Size: 32 1423 30 2 30 2386
Fragments arranged by size:
2386 1446 1423 262 32 30 30 30
19 13 13 2 2 2
HincII GTy'rAC
Cuts at: 357 2621 2729 357
Size: 2264 108 3318
Fragments arranged by size:
3318 2264 108
KasI G'GCGC_C
Cuts at: 501 2508 3120 3368 501
Size: 2007 612 248 2823
Fragments arranged by size:
2823 2007 612 248
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MluI A'CGCG_T
Cuts at: 5123 5123
Size: 5690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1292 4449 4633 1292
Size: 3157 184 2349
Fragments arranged by size:
3157 2349 184
MscI TGG'CCA
Cuts at: 2236 2248 3451 2236
Size: 12 1203 4475
Fragments arranged by size:
4475 1203 12
MslI CAynn'nnrTG
Cuts at: 668 733 1594 2242 3679 3806 668
Size: 65 861 648 1437 127 2552
Fragments arranged by size:
2552 1437 861 648 127 65
MspA1I CmG'CkG
Cuts at: 668 1102 1302 1387 1474 1594 2801 3115 3475
Size: 434 200 85 87 120 1207 314 360
Cuts at: 3475 4259 4504 668
Size: 784 245 1854
Fragments arranged by size:
1854 1207 784 434 360 314 245 200
120 87 85
NaeI GCC'GGC
Cuts at: 2226 3871 2226
Size: 1645 4045
NarI GG'CG_CC
Cuts at: 502 2509 3121 3369 502
Size: 2007 612 248 2823
Fragments arranged by size:
2823 2007 612 248
NcoI C'CATG_G
Cuts at: 917 2165 2231 917
Size: 1248 66 4376
Fragments arranged by size:
4376 1248 66
NgoMIV G'CCGG_C
Cuts at: 2224 3869 2224
Size: 1645 4045
NheI G'CTAG_C
Cuts at: 1708 1708
Size: 5690
NspI r_CATG'y
Cuts at: 1066 2743 3243 4846 1066
Size: 1677 500 1603 1910
Fragments arranged by size:
1910 1677 1603 500
PasI CC'CwG_GG
Cuts at: 138 1224 1313 1564 1616 1703 138
Size: 1086 89 251 52 87 4125
Fragments arranged by size:
4125 1086 251 89 87 52
PciI A'CATG_T
Cuts at: 4842 4842
Size: 5690
PflMI CCAn_nnn'nTGG
Cuts at: 585 1494 1681 2279 585
Size: 909 187 598 3996
Fragments arranged by size:
3996 909 598 187
PfoI T'CCnGG_A
Cuts at: 515 715 1493 2155 515
Size: 200 778 662 4050
Fragments arranged by size:
4050 778 662 200
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 625 657 4136 4168 625
Size: 32 3479 32 2147
Fragments arranged by size:
3479 2147 32 32
PpuMI rG'GwC_Cy
Cuts at: 713 1309 1612 1871 2304 713
Size: 596 303 259 433 4099
Fragments arranged by size:
4099 596 433 303 259
PstI C_TGCA'G
Cuts at: 632 2737 3422 632
Size: 2105 685 2900
Fragments arranged by size:
2900 2105 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 1102 1302 1387 1594 3115 3475 1102
Size: 200 85 207 1521 360 3317
Fragments arranged by size:
3317 1521 360 207 200 85
RsrII CG'GwC_CG
Cuts at: 3885 3885
Size: 5690
SacI G_AGCT'C
Cuts at: 2481 2639 2704 2481
Size: 158 65 5467
Fragments arranged by size:
5467 158 65
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SanDI GG'GwC_CC
Cuts at: 1309 1871 1309
Size: 562 5128
SapI GCTCTTCn'nnn_
Cuts at: 383 3713 3923 383
Size: 3330 210 2150
Fragments arranged by size:
3330 2150 210
SbfI CC_TGCA'GG
Cuts at: 2737 2737
Size: 5690
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 1922 1922
Size: 5690
SfcI C'TryA_G
Cuts at: 33 254 628 954 2733 3418 4386 4577 33
Size: 221 374 326 1779 685 968 191 1146
Fragments arranged by size:
1779 1146 968 685 374 326 221 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 133 133
Size: 5690
SfoI GGC'GCC
Cuts at: 503 2510 3122 3370 503
Size: 2007 612 248 2823
Fragments arranged by size:
2823 2007 612 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SmaI CCC'GGG
Cuts at: 2712 2712
Size: 5690
SmlI C'TyrA_G
Cuts at: 1246 1999 2122 2374 2638 2845 3760 4474 4736
Size: 753 123 252 264 207 915 714 262
Cuts at: 4736 1246
Size: 2200
Fragments arranged by size:
2200 915 753 714 264 262 252 207
123
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 2743 3243 2743
Size: 500 5190
SspI AAT'ATT
Cuts at: 1633 1633
Size: 5690
StyI C'CwwG_G
Cuts at: 743 917 2081 2165 2189 2231 2824 3178 743
Size: 174 1164 84 24 42 593 354 3255
Fragments arranged by size:
3255 1164 593 354 174 84 42 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 499 1232 1688 1711 1957 2203 2514 3273 3406
Size: 733 456 23 246 246 311 759 133
Cuts at: 3406 3865 4074 499
Size: 459 209 2115
Fragments arranged by size:
2115 759 733 459 456 311 246 246
209 133 23
TatI w'GTAC_w
Cuts at: 1065 1775 5205 1065
Size: 710 3430 1550
Fragments arranged by size:
3430 1550 710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1328 1360 5207 5239 1328
Size: 32 3847 32 1779
Fragments arranged by size:
3847 1779 32 32
Tth111I GACn'n_nGTC
Cuts at: 1510 3487 1510
Size: 1977 3713
XbaI T'CTAG_A
Cuts at: 2721 2721
Size: 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 1134 1678 1923 2016 2208 3185 219
Size: 915 544 245 93 192 977 2724
Fragments arranged by size:
2724 977 915 544 245 192 93
XmaI C'CCGG_G
Cuts at: 2710 2710
Size: 5690
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BseRI BsiWI
BsmI BspEI BssHII ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI