Restriction map for
pF1KA1777
(Circular) (Six-base) MAPSORT of: pF1KA1777.seq Check: 7618 from: 1 to: 5957
pF1KA1777 5957 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 790 790
Size: 5957
AatII G_ACGT'C
Cuts at: 1334 1334
Size: 5957
AccI GT'mk_AC
Cuts at: 839 2995 839
Size: 2156 3801
Acc65I G'GTAC_C
Cuts at: 520 2973 520
Size: 2453 3504
AclI AA'CG_TT
Cuts at: 915 5797 915
Size: 4882 1075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 1916 2185 2326 3782 4214 4567 5229 410
Size: 1506 269 141 1456 432 353 662 1138
Fragments arranged by size:
1506 1456 1138 662 432 353 269 141
AflIII A'CryG_T
Cuts at: 3309 5109 5390 3309
Size: 1800 281 3876
Fragments arranged by size:
3876 1800 281
AleI CACnn'nnGTG
Cuts at: 312 832 312
Size: 520 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 330 330
Size: 5957
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 584 616 1844 1876 2398 2430 2528 2560 584
Size: 32 1228 32 522 32 98 32 3981
Fragments arranged by size:
3981 1228 522 98 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 199 241 2295 2448 3262 4700 199
Size: 42 2054 153 814 1438 1456
Fragments arranged by size:
2054 1456 1438 814 153 42
ApaI G_GGCC'C
Cuts at: 479 479
Size: 5957
ApaLI G'TGCA_C
Cuts at: 1086 4795 1086
Size: 3709 2248
ApoI r'AATT_y
Cuts at: 1117 1413 1815 2648 2751 2961 5310 5566 1117
Size: 296 402 833 103 210 2349 256 1508
Fragments arranged by size:
2349 1508 833 402 296 256 210 103
AseI AT'TA_AT
Cuts at: 20 20
Size: 5957
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
AvaI C'yCGr_G
Cuts at: 478 1101 1242 2977 478
Size: 623 141 1735 3458
Fragments arranged by size:
3458 1735 623 141
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 504 537 504
Size: 33 5924
BamHI G'GATC_C
Cuts at: 1076 2982 1076
Size: 1906 4051
BanI G'GyrC_C
Cuts at: 491 520 1798 2973 3387 3635 3670 491
Size: 29 1278 1175 414 248 35 2778
Fragments arranged by size:
2778 1278 1175 414 248 35 29
BanII G_rGCy'C
Cuts at: 412 479 689 1496 1555 2971 4001 412
Size: 67 210 807 59 1416 1030 2368
Fragments arranged by size:
2368 1416 1030 807 210 67 59
BbeI G_GCGC'C
Cuts at: 3391 3639 3391
Size: 248 5709
BbsI GAAGACnn'nnnn_
Cuts at: 851 2332 851
Size: 1481 4476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 743 777 743
Size: 34 5923
BciVI GTATCCnnnnn_n'
Cuts at: 3855 4911 5957 3855
Size: 1056 1046 3855
Fragments arranged by size:
3855 1056 1046
BclI T'GATC_A
Cuts at: 740 3477 740
Size: 2737 3220
BfrBI ATG'CAT
Cuts at: 1656 1656
Size: 5957
BglI GCCn_nnn'nGGC
Cuts at: 1264 1264
Size: 5957
BglII A'GATC_T
Cuts at: 1431 3472 5509 1431
Size: 2041 2037 1879
Fragments arranged by size:
2041 2037 1879
BlpI GC'TnA_GC
Cuts at: 3069 3069
Size: 5957
Bme1580I G_kGCm'C
Cuts at: 479 1090 2856 3582 3675 4799 479
Size: 611 1766 726 93 1124 1637
Fragments arranged by size:
1766 1637 1124 726 611 93
BmgBI CAC'GTC
Cuts at: 384 384
Size: 5957
BmrI ACTGGGnnnn_n'
Cuts at: 3169 3250 3585 5729 3169
Size: 81 335 2144 3397
Fragments arranged by size:
3397 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 402 4324 402
Size: 3922 2035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 1898 2150 2397 2488 1846
Size: 52 252 247 91 5315
Fragments arranged by size:
5315 252 247 91 52
Bpu10I CC'TnA_GC
Cuts at: 411 411
Size: 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2235 3133 4012 4726 5024 2235
Size: 898 879 714 298 3168
Fragments arranged by size:
3168 898 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5661 5661
Size: 5957
BsaAI yAC'GTr
Cuts at: 843 3310 843
Size: 2467 3490
BsaBI GATnn'nnATC
Cuts at: 6 2831 3471 5508 6
Size: 2825 640 2037 455
Fragments arranged by size:
2825 2037 640 455
BsaHI Gr'CG_yC
Cuts at: 1331 3388 3636 4338 5834 1331
Size: 2057 248 702 1496 1454
Fragments arranged by size:
2057 1496 1454 702 248
BsaWI w'CCGG_w
Cuts at: 3146 3667 4345 4756 4903 5177 3146
Size: 521 678 411 147 274 3926
Fragments arranged by size:
3926 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 1299 1329 1844 1874 2528 2558 163
Size: 30 1106 30 515 30 654 30 3562
Fragments arranged by size:
3562 1106 654 515 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 825 932 1825 280
Size: 545 107 893 4412
Fragments arranged by size:
4412 893 545 107
BseYI C'CCAG_C
Cuts at: 148 936 1022 1855 2492 2906 3382 4140 4805
Size: 788 86 833 637 414 476 758 665
Cuts at: 4805 5213 148
Size: 408 892
Fragments arranged by size:
892 833 788 758 665 637 476 414
408 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 912 978 1000 1137 1982 2434 2451 2572
Size: 302 66 22 137 845 452 17 121
Cuts at: 2572 2606 610
Size: 34 3961
Fragments arranged by size:
3961 845 452 302 137 121 66 34
22 17
BsiEI CG_ry'CG
Cuts at: 104 3499 3545 4775 104
Size: 3395 46 1230 1286
Fragments arranged by size:
3395 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 383 412 1090 1496 1555 1886 2099 2971 3749
Size: 29 678 406 59 331 213 872 778
Cuts at: 3749 3939 4799 383
Size: 190 860 1541
Fragments arranged by size:
1541 872 860 778 678 406 331 213
190 59 29
BsmI GAATG_Cn'
Cuts at: 1224 2513 1224
Size: 1289 4668
BsmBI CGTCTCn'nnnn_
Cuts at: 390 390
Size: 5957
Bsp1286I G_dGCh'C
Cuts at: 383 412 479 689 1090 1496 1555 1886 2099
Size: 29 67 210 401 406 59 331 213
Cuts at: 2099 2856 2971 3582 3675 3749 3939 4001 4799
Size: 757 115 611 93 74 190 62 798
Cuts at: 4799 383
Size: 1541
Fragments arranged by size:
1541 798 757 611 406 401 331 213
210 190 115 93 74 67 62 59
29
BspHI T'CATG_A
Cuts at: 1191 1496 4389 1191
Size: 305 2893 2759
Fragments arranged by size:
2893 2759 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 934 1000 3007 3221 3523 3904 790
Size: 144 66 2007 214 302 381 2843
Fragments arranged by size:
2843 2007 381 302 214 144 66
BsrBI CCG'CTC
Cuts at: 50 4249 4303 5787 5952 50
Size: 4199 54 1484 165 55
Fragments arranged by size:
4199 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 891 1229 3869 891
Size: 338 2640 2979
Fragments arranged by size:
2979 2640 338
BsrFI r'CCGG_y
Cuts at: 2438 3955 4136 2438
Size: 1517 181 4259
Fragments arranged by size:
4259 1517 181
BssSI C'ACGA_G
Cuts at: 4228 4936 4228
Size: 708 5249
BstAPI GCAn_nnn'nTGC
Cuts at: 2448 2510 3334 2448
Size: 62 824 5071
Fragments arranged by size:
5071 824 62
BstBI TT'CG_AA
Cuts at: 4318 4318
Size: 5957
BstXI CCAn_nnnn'nTGG
Cuts at: 114 801 834 1870 2375 114
Size: 687 33 1036 505 3696
Fragments arranged by size:
3696 1036 687 505 33
BstYI r'GATC_y
Cuts at: 1 1076 1431 2540 2982 3449 3472 3807 4053
Size: 1075 355 1109 442 467 23 335 246
Cuts at: 4053 4359 4371 4457 5509 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1109 1075 1052 467 449 442 355 335
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 471 1596 1626 471
Size: 1125 30 4802
Fragments arranged by size:
4802 1125 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3907 4088 4251 3907
Size: 181 163 5613
Fragments arranged by size:
5613 181 163
BtsI GCAGTG_nn'
Cuts at: 247 627 2393 3200 247
Size: 380 1766 807 3004
Fragments arranged by size:
3004 1766 807 380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1920 1955 1920
Size: 35 5922
DraI TTT'AAA
Cuts at: 1997 2956 1997
Size: 959 4998
DraIII CAC_nnn'GTG
Cuts at: 1798 1798
Size: 5957
DrdI GACnn_nn'nnGTC
Cuts at: 873 1466 1924 3663 5007 873
Size: 593 458 1739 1344 1823
Fragments arranged by size:
1823 1739 1344 593 458
EaeI y'GGCC_r
Cuts at: 824 1265 1737 3542 3716 4107 4134 5479 824
Size: 441 472 1805 174 391 27 1345 1302
Fragments arranged by size:
1805 1345 1302 472 441 391 174 27
EagI C'GGCC_G
Cuts at: 3542 3542
Size: 5957
EarI CTCTTCn'nnn_
Cuts at: 386 677 1193 1347 1996 2855 3980 4190 386
Size: 291 516 154 649 859 1125 210 2153
Fragments arranged by size:
2153 1125 859 649 516 291 210 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4165 4907 5053 5767 4165
Size: 742 146 714 4355
Fragments arranged by size:
4355 742 714 146
EcoICRI GAG'CTC
Cuts at: 410 1494 1553 2969 410
Size: 1084 59 1416 3398
Fragments arranged by size:
3398 1416 1084 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 1846 1898 1916 2150 2185 2326 2397 2488
Size: 1436 52 18 234 35 141 71 91
Cuts at: 2488 3782 4214 4567 5229 410
Size: 1294 432 353 662 1138
Fragments arranged by size:
1436 1294 1138 662 432 353 234 141
91 71 52 35 18
EcoO109I rG'GnC_Cy
Cuts at: 956 1476 2114 3096 956
Size: 520 638 982 3817
Fragments arranged by size:
3817 982 638 520
EcoRI G'AATT_C
Cuts at: 1413 1815 2961 1413
Size: 402 1146 4409
Fragments arranged by size:
4409 1146 402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3976 3976
Size: 5957
FspI TGC'GCA
Cuts at: 550 3738 550
Size: 3188 2769
FspAI rTGC'GCAy
Cuts at: 550 550
Size: 5957
HaeII r_GCGC'y
Cuts at: 1755 3391 3639 4869 5640 1755
Size: 1636 248 1230 771 2072
Fragments arranged by size:
2072 1636 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 237 269 394 426 958 990 2123
Size: 32 42 32 125 32 532 32 1133
Cuts at: 2123 2155 2570 2602 2824 2856 3799 3829 3831
Size: 32 415 32 222 32 943 30 2
Cuts at: 3831 3861 163
Size: 30 2259
Fragments arranged by size:
2259 1133 943 532 415 222 125 42
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2996 2996
Size: 5957
KasI G'GCGC_C
Cuts at: 3387 3635 3387
Size: 248 5709
KpnI G_GTAC'C
Cuts at: 524 2977 524
Size: 2453 3504
MluI A'CGCG_T
Cuts at: 5390 5390
Size: 5957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 1508 4716 4900 149
Size: 1359 3208 184 1206
Fragments arranged by size:
3208 1359 1206 184
MscI TGG'CCA
Cuts at: 1739 3718 1739
Size: 1979 3978
MslI CAynn'nnrTG
Cuts at: 312 784 832 1537 2282 2822 2828 3946 4073
Size: 472 48 705 745 540 6 1118 127
Cuts at: 4073 312
Size: 2196
Fragments arranged by size:
2196 1118 745 705 540 472 127 48
6
MspA1I CmG'CkG
Cuts at: 1457 2496 2670 3068 3382 3742 4526 4771 1457
Size: 1039 174 398 314 360 784 245 2643
Fragments arranged by size:
2643 1039 784 398 360 314 245 174
NaeI GCC'GGC
Cuts at: 4138 4138
Size: 5957
NarI GG'CG_CC
Cuts at: 3388 3636 3388
Size: 248 5709
NcoI C'CATG_G
Cuts at: 471 1626 471
Size: 1155 4802
NgoMIV G'CCGG_C
Cuts at: 4136 4136
Size: 5957
NsiI A_TGCA'T
Cuts at: 1658 1658
Size: 5957
NspI r_CATG'y
Cuts at: 2190 3010 3510 5113 2190
Size: 820 500 1603 3034
Fragments arranged by size:
3034 1603 820 500
PasI CC'CwG_GG
Cuts at: 1569 1569
Size: 5957
PciI A'CATG_T
Cuts at: 5109 5109
Size: 5957
PflMI CCAn_nnn'nTGG
Cuts at: 501 501
Size: 5957
PfoI T'CCnGG_A
Cuts at: 862 919 1079 1612 2099 2294 862
Size: 57 160 533 487 195 4525
Fragments arranged by size:
4525 533 487 195 160 57
PmeI GTTT'AAAC
Cuts at: 2956 2956
Size: 5957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1844 1876 2528 2560 4403 4435 1844
Size: 32 652 32 1843 32 3366
Fragments arranged by size:
3366 1843 652 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1476 2114 1476
Size: 638 5319
PspOMI G'GGCC_C
Cuts at: 475 475
Size: 5957
PstI C_TGCA'G
Cuts at: 1054 1475 3004 3689 1054
Size: 421 1529 685 3322
Fragments arranged by size:
3322 1529 685 421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5957
PvuII CAG'CTG
Cuts at: 2496 3382 3742 2496
Size: 886 360 4711
Fragments arranged by size:
4711 886 360
RsrII CG'GwC_CG
Cuts at: 4152 4152
Size: 5957
SacI G_AGCT'C
Cuts at: 412 1496 1555 2971 412
Size: 1084 59 1416 3398
Fragments arranged by size:
3398 1416 1084 59
SalI G'TCGA_C
Cuts at: 2994 2994
Size: 5957
SapI GCTCTTCn'nnn_
Cuts at: 677 3980 4190 677
Size: 3303 210 2444
Fragments arranged by size:
3303 2444 210
SbfI CC_TGCA'GG
Cuts at: 3004 3004
Size: 5957
ScaI AGT'ACT
Cuts at: 5474 5474
Size: 5957
SfcI C'TryA_G
Cuts at: 33 1050 1471 2931 3000 3685 4653 4844 33
Size: 1017 421 1460 69 685 968 191 1146
Fragments arranged by size:
1460 1146 1017 968 685 421 191 69
SfoI GGC'GCC
Cuts at: 3389 3637 3389
Size: 248 5709
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
SmaI CCC'GGG
Cuts at: 2979 2979
Size: 5957
SmlI C'TyrA_G
Cuts at: 2214 3112 4027 4741 5003 2214
Size: 898 915 714 262 3168
Fragments arranged by size:
3168 915 898 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5957
SphI G_CATG'C
Cuts at: 3010 3510 3010
Size: 500 5457
StuI AGG'CCT
Cuts at: 541 541
Size: 5957
StyI C'CwwG_G
Cuts at: 471 1626 1845 3091 3445 471
Size: 1155 219 1246 354 2983
Fragments arranged by size:
2983 1246 1155 354 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1240 2506 3540 3673 4132 4341 1240
Size: 1266 1034 133 459 209 2856
Fragments arranged by size:
2856 1266 1034 459 209 133
TatI w'GTAC_w
Cuts at: 621 1238 1648 5472 621
Size: 617 410 3824 1106
Fragments arranged by size:
3824 1106 617 410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 962 994 1299 1331 1713 1745 1893
Size: 32 455 32 305 32 382 32 148
Cuts at: 1893 1925 5474 5506 475
Size: 32 3549 32 926
Fragments arranged by size:
3549 926 455 382 305 148 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1503 3754 1503
Size: 2251 3706
XbaI T'CTAG_A
Cuts at: 2988 2988
Size: 5957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1318 3452 1318
Size: 2134 3823
XmaI C'CCGG_G
Cuts at: 2977 2977
Size: 5957
XmnI GAAnn'nnTTC
Cuts at: 215 215
Size: 5957
ZraI GAC'GTC
Cuts at: 1332 1332
Size: 5957
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI AvrII BbvCI BmtI
BsiWI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI