Restriction map for
pF1KA1779
(Circular) (Six-base) MAPSORT of: pF1KA1779.seq Check: 1940 from: 1 to: 6743
pF1KA1779 6743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2499 2499
Size: 6743
AccI GT'mk_AC
Cuts at: 3781 3781
Size: 6743
Acc65I G'GTAC_C
Cuts at: 3495 3759 3495
Size: 264 6479
AclI AA'CG_TT
Cuts at: 6583 6583
Size: 6743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 458 1070 1792 2236 2801 2905 4568 5000
Size: 174 612 722 444 565 104 1663 432
Cuts at: 5000 5353 6015 284
Size: 353 662 1012
Fragments arranged by size:
1663 1012 722 662 612 565 444 432
353 174 104
AfeI AGC'GCT
Cuts at: 3099 3153 3099
Size: 54 6689
AflIII A'CryG_T
Cuts at: 2142 2972 4095 5895 6176 2142
Size: 830 1123 1800 281 2709
Fragments arranged by size:
2709 1800 1123 830 281
AgeI A'CCGG_T
Cuts at: 3040 3040
Size: 6743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1514 2454 1514
Size: 940 5803
AlwNI CAG_nnn'CTG
Cuts at: 1014 1977 2161 3720 4048 5486 1014
Size: 963 184 1559 328 1438 2271
Fragments arranged by size:
2271 1559 1438 963 328 184
ApaLI G'TGCA_C
Cuts at: 541 3044 5581 541
Size: 2503 2537 1703
Fragments arranged by size:
2537 2503 1703
ApoI r'AATT_y
Cuts at: 1328 2134 2209 3747 6096 6352 1328
Size: 806 75 1538 2349 256 1719
Fragments arranged by size:
2349 1719 1538 806 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 6743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6743
AvaI C'yCGr_G
Cuts at: 133 193 2472 3763 133
Size: 60 2279 1291 3113
Fragments arranged by size:
3113 2279 1291 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 491 524 491
Size: 33 6710
BamHI G'GATC_C
Cuts at: 746 908 3768 746
Size: 162 2860 3721
Fragments arranged by size:
3721 2860 162
BanI G'GyrC_C
Cuts at: 1448 2502 2870 3495 3759 4173 4421 4456 1448
Size: 1054 368 625 264 414 248 35 3735
Fragments arranged by size:
3735 1054 625 414 368 264 248 35
BanII G_rGCy'C
Cuts at: 193 666 1249 1694 1968 2093 2153 2479 3443
Size: 473 583 445 274 125 60 326 964
Cuts at: 3443 3757 4787 193
Size: 314 1030 2149
Fragments arranged by size:
2149 1030 964 583 473 445 326 314
274 125 60
BbeI G_GCGC'C
Cuts at: 4177 4425 4177
Size: 248 6495
BbsI GAAGACnn'nnnn_
Cuts at: 590 767 1853 3536 3734 590
Size: 177 1086 1683 198 3599
Fragments arranged by size:
3599 1683 1086 198 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1219 1253 90
Size: 34 1095 34 5580
Fragments arranged by size:
5580 1095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2658 4641 5697 6743 2658
Size: 1983 1056 1046 2658
Fragments arranged by size:
2658 1983 1056 1046
BclI T'GATC_A
Cuts at: 4263 4263
Size: 6743
BglII A'GATC_T
Cuts at: 4258 6295 4258
Size: 2037 4706
BlpI GC'TnA_GC
Cuts at: 665 1993 3855 665
Size: 1328 1862 3553
Fragments arranged by size:
3553 1862 1328
Bme1580I G_kGCm'C
Cuts at: 545 1451 2507 2873 2980 3048 4368 4461 5585
Size: 906 1056 366 107 68 1320 93 1124
Cuts at: 5585 545
Size: 1703
Fragments arranged by size:
1703 1320 1124 1056 906 366 107 93
68
BmrI ACTGGGnnnn_n'
Cuts at: 907 3564 3955 4036 4371 6515 907
Size: 2657 391 81 335 2144 1135
Fragments arranged by size:
2657 2144 1135 391 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 5110 2816
Size: 2294 4449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1218 1416 2936 3468 1218
Size: 198 1520 532 4493
Fragments arranged by size:
4493 1520 532 198
Bpu10I CC'TnA_GC
Cuts at: 149 1033 3470 3602 149
Size: 884 2437 132 3290
Fragments arranged by size:
3290 2437 884 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 611 3623 3919 4798 5512 5810 472
Size: 139 3012 296 879 714 298 1405
Fragments arranged by size:
3012 1405 879 714 298 296 139
BsaI GGTCTCn'nnnn_
Cuts at: 6447 6447
Size: 6743
BsaAI yAC'GTr
Cuts at: 1359 2145 2975 4096 1359
Size: 786 830 1121 4006
Fragments arranged by size:
4006 1121 830 786
BsaBI GATnn'nnATC
Cuts at: 6 4257 6294 6
Size: 4251 2037 455
Fragments arranged by size:
4251 2037 455
BsaHI Gr'CG_yC
Cuts at: 595 4174 4422 5124 6620 595
Size: 3579 248 702 1496 718
Fragments arranged by size:
3579 1496 718 702 248
BsaWI w'CCGG_w
Cuts at: 723 794 2592 3040 3932 4453 5131 5542 5689
Size: 71 1798 448 892 521 678 411 147
Cuts at: 5689 5963 723
Size: 274 1503
Fragments arranged by size:
1798 1503 892 678 521 448 411 274
147 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2833 2863 2833
Size: 30 6713
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 893 1419 1474 1827 1973 1976 2448 2854
Size: 424 526 55 353 146 3 472 406
Cuts at: 2854 3607 469
Size: 753 3605
Fragments arranged by size:
3605 753 526 472 424 406 353 146
55 3
BseYI C'CCAG_C
Cuts at: 833 993 1905 2506 2529 2544 2640 3229 3283
Size: 160 912 601 23 15 96 589 54
Cuts at: 3283 4168 4926 5591 5999 833
Size: 885 758 665 408 1577
Fragments arranged by size:
1577 912 885 758 665 601 589 408
160 96 54 23 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1027 2573 689
Size: 338 1546 4859
Fragments arranged by size:
4859 1546 338
BsiEI CG_ry'CG
Cuts at: 104 2323 2327 4285 4331 5561 104
Size: 2219 4 1958 46 1230 1286
Fragments arranged by size:
2219 1958 1286 1230 46 4
BsiHKAI G_wGCw'C
Cuts at: 423 545 666 723 1721 1968 3048 3757 4535
Size: 122 121 57 998 247 1080 709 778
Cuts at: 4535 4725 5585 423
Size: 190 860 1581
Fragments arranged by size:
1581 1080 998 860 778 709 247 190
122 121 57
BsmI GAATG_Cn'
Cuts at: 3476 3476
Size: 6743
BsmBI CGTCTCn'nnnn_
Cuts at: 132 309 1456 132
Size: 177 1147 5419
Fragments arranged by size:
5419 1147 177
Bsp1286I G_dGCh'C
Cuts at: 193 423 545 666 723 1249 1451 1694 1721
Size: 230 122 121 57 526 202 243 27
Cuts at: 1721 1968 2093 2153 2479 2507 2873 2980 3048
Size: 247 125 60 326 28 366 107 68
Cuts at: 3048 3443 3757 4368 4461 4535 4725 4787 5585
Size: 395 314 611 93 74 190 62 798
Cuts at: 5585 193
Size: 1351
Fragments arranged by size:
1351 798 611 526 395 366 326 314
247 243 230 202 190 125 122 121
107 93 74 68 62 60 57 28
27
BspEI T'CCGG_A
Cuts at: 794 794
Size: 6743
BspHI T'CATG_A
Cuts at: 3482 5175 3482
Size: 1693 5050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 623 1049 1482 2499 3793 4007 4309 4690 623
Size: 426 433 1017 1294 214 302 381 2676
Fragments arranged by size:
2676 1294 1017 433 426 381 302 214
BsrBI CCG'CTC
Cuts at: 50 183 5035 5089 6573 6738 50
Size: 133 4852 54 1484 165 55
Fragments arranged by size:
4852 1484 165 133 55 54
BsrDI GCAATG_nn'
Cuts at: 2795 4655 2795
Size: 1860 4883
BsrFI r'CCGG_y
Cuts at: 2326 2395 3040 4741 4922 2326
Size: 69 645 1701 181 4147
Fragments arranged by size:
4147 1701 645 181 69
BsrGI T'GTAC_A
Cuts at: 443 819 1682 1816 443
Size: 376 863 134 5370
Fragments arranged by size:
5370 863 376 134
BssSI C'ACGA_G
Cuts at: 5014 5722 5014
Size: 708 6035
BstAPI GCAn_nnn'nTGC
Cuts at: 4120 4120
Size: 6743
BstBI TT'CG_AA
Cuts at: 1239 5104 1239
Size: 3865 2878
BstEII G'GTnAC_C
Cuts at: 997 3059 3275 997
Size: 2062 216 4465
Fragments arranged by size:
4465 2062 216
BstYI r'GATC_y
Cuts at: 1 746 908 1973 2222 3768 4235 4258 4593
Size: 745 162 1065 249 1546 467 23 335
Cuts at: 4593 4839 5145 5157 5243 6295 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1546 1065 1052 745 467 449 335 306
249 246 162 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 425 1007 425
Size: 582 6161
BtgI C'CryG_G
Cuts at: 114 750 1982 2450 2498 3053 3161 3215 3269
Size: 636 1232 468 48 555 108 54 54
Cuts at: 3269 114
Size: 3588
Fragments arranged by size:
3588 1232 636 555 468 108 54 54
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1407 2002 4693 4874 5037 1407
Size: 595 2691 181 163 3113
Fragments arranged by size:
3113 2691 595 181 163
BtsI GCAGTG_nn'
Cuts at: 653 989 3024 3986 653
Size: 336 2035 962 3410
Fragments arranged by size:
3410 2035 962 336
ClaI AT'CG_AT
Cuts at: 1027 1027
Size: 6743
DraI TTT'AAA
Cuts at: 2139 3742 2139
Size: 1603 5140
DraIII CAC_nnn'GTG
Cuts at: 1455 1455
Size: 6743
DrdI GACnn_nn'nnGTC
Cuts at: 2698 4449 5793 2698
Size: 1751 1344 3648
Fragments arranged by size:
3648 1751 1344
EaeI y'GGCC_r
Cuts at: 461 2324 3698 4328 4502 4893 4920 6265 461
Size: 1863 1374 630 174 391 27 1345 939
Fragments arranged by size:
1863 1374 1345 939 630 391 174 27
EagI C'GGCC_G
Cuts at: 2324 4328 2324
Size: 2004 4739
EarI CTCTTCn'nnn_
Cuts at: 1768 2086 2183 2559 2763 2921 3431 4766 4976
Size: 318 97 376 204 158 510 1335 210
Cuts at: 4976 1768
Size: 3535
Fragments arranged by size:
3535 1335 510 376 318 210 204 158
97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 235 1486 4951 5693 5839 6553 235
Size: 1251 3465 742 146 714 425
Fragments arranged by size:
3465 1251 742 714 425 146
EcoICRI GAG'CTC
Cuts at: 664 1966 3755 664
Size: 1302 1789 3652
Fragments arranged by size:
3652 1789 1302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 458 1070 1218 1416 1792 2236 2801 2905
Size: 174 612 148 198 376 444 565 104
Cuts at: 2905 2936 3468 4568 5000 5353 6015 284
Size: 31 532 1100 432 353 662 1012
Fragments arranged by size:
1100 1012 662 612 565 532 444 432
376 353 198 174 148 104 31
EcoNI CCTnn'n_nnAGG
Cuts at: 1006 1006
Size: 6743
EcoO109I rG'GnC_Cy
Cuts at: 159 1011 2514 3882 159
Size: 852 1503 1368 3020
Fragments arranged by size:
3020 1503 1368 852
EcoRI G'AATT_C
Cuts at: 3747 3747
Size: 6743
EcoRV GAT'ATC
Cuts at: 2768 2768
Size: 6743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4762 4762
Size: 6743
FspI TGC'GCA
Cuts at: 4524 4524
Size: 6743
HaeII r_GCGC'y
Cuts at: 3101 3155 4177 4425 5655 6426 3101
Size: 54 1022 248 1230 771 3418
Fragments arranged by size:
3418 1230 1022 771 248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 393 425 558 590 1142 1174 2207 2239 3064
Size: 32 133 32 552 32 1033 32 825
Cuts at: 3064 3096 3621 3653 4585 4615 4617 4647 393
Size: 32 525 32 932 30 2 30 2489
Fragments arranged by size:
2489 1033 932 825 552 525 133 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 612 3782 612
Size: 3170 3573
KasI G'GCGC_C
Cuts at: 4173 4421 4173
Size: 248 6495
KpnI G_GTAC'C
Cuts at: 3499 3763 3499
Size: 264 6479
MluI A'CGCG_T
Cuts at: 6176 6176
Size: 6743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2821 5502 5686 2821
Size: 2681 184 3878
Fragments arranged by size:
3878 2681 184
MscI TGG'CCA
Cuts at: 463 4504 463
Size: 4041 2702
MslI CAynn'nnrTG
Cuts at: 1987 3481 4732 4859 1987
Size: 1494 1251 127 3871
Fragments arranged by size:
3871 1494 1251 127
MspA1I CmG'CkG
Cuts at: 116 362 703 1373 1397 1445 2158 2452 3688
Size: 246 341 670 24 48 713 294 1236
Cuts at: 3688 3734 3854 4168 4528 5312 5557 116
Size: 46 120 314 360 784 245 1302
Fragments arranged by size:
1302 1236 784 713 670 360 341 314
294 246 245 120 48 46 24
NaeI GCC'GGC
Cuts at: 2328 4924 2328
Size: 2596 4147
NarI GG'CG_CC
Cuts at: 4174 4422 4174
Size: 248 6495
NcoI C'CATG_G
Cuts at: 2498 3215 2498
Size: 717 6026
NgoMIV G'CCGG_C
Cuts at: 2326 4922 2326
Size: 2596 4147
NspI r_CATG'y
Cuts at: 444 2109 3796 4296 5899 444
Size: 1665 1687 500 1603 1288
Fragments arranged by size:
1687 1665 1603 1288 500
PasI CC'CwG_GG
Cuts at: 162 949 3068 162
Size: 787 2119 3837
Fragments arranged by size:
3837 2119 787
PciI A'CATG_T
Cuts at: 5895 5895
Size: 6743
PflMI CCAn_nnn'nTGG
Cuts at: 1936 1936
Size: 6743
PmeI GTTT'AAAC
Cuts at: 3742 3742
Size: 6743
PmlI CAC'GTG
Cuts at: 1359 2145 2975 1359
Size: 786 830 5127
Fragments arranged by size:
5127 830 786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2415 5189 5221 2383
Size: 32 2774 32 3905
Fragments arranged by size:
3905 2774 32 32
PpuMI rG'GwC_Cy
Cuts at: 1011 1011
Size: 6743
PspXI vC'TCGA_Gb
Cuts at: 2472 2472
Size: 6743
PstI C_TGCA'G
Cuts at: 209 745 2335 3790 4475 209
Size: 536 1590 1455 685 2477
Fragments arranged by size:
2477 1590 1455 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6743
PvuII CAG'CTG
Cuts at: 703 3688 3734 4168 4528 703
Size: 2985 46 434 360 2918
Fragments arranged by size:
2985 2918 434 360 46
RsrII CG'GwC_CG
Cuts at: 4938 4938
Size: 6743
SacI G_AGCT'C
Cuts at: 666 1968 3757 666
Size: 1302 1789 3652
Fragments arranged by size:
3652 1789 1302
SacII CC_GC'GG
Cuts at: 117 2453 117
Size: 2336 4407
SalI G'TCGA_C
Cuts at: 3780 3780
Size: 6743
SapI GCTCTTCn'nnn_
Cuts at: 1768 2183 3431 4766 4976 1768
Size: 415 1248 1335 210 3535
Fragments arranged by size:
3535 1335 1248 415 210
SbfI CC_TGCA'GG
Cuts at: 3790 3790
Size: 6743
ScaI AGT'ACT
Cuts at: 6260 6260
Size: 6743
SexAI A'CCwGG_T
Cuts at: 3491 3491
Size: 6743
SfcI C'TryA_G
Cuts at: 33 205 741 2238 2331 3786 4471 5439 5630
Size: 172 536 1497 93 1455 685 968 191
Cuts at: 5630 33
Size: 1146
Fragments arranged by size:
1497 1455 1146 968 685 536 191 172
93
SfoI GGC'GCC
Cuts at: 4175 4423 4175
Size: 248 6495
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6743
SmaI CCC'GGG
Cuts at: 135 3765 135
Size: 3630 3113
SmlI C'TyrA_G
Cuts at: 451 626 2472 3638 3898 4813 5527 5789 451
Size: 175 1846 1166 260 915 714 262 1405
Fragments arranged by size:
1846 1405 1166 915 714 262 260 175
SpeI A'CTAG_T
Cuts at: 67 3349 67
Size: 3282 3461
SphI G_CATG'C
Cuts at: 3796 4296 3796
Size: 500 6243
SspI AAT'ATT
Cuts at: 2281 2281
Size: 6743
StuI AGG'CCT
Cuts at: 2600 2600
Size: 6743
StyI C'CwwG_G
Cuts at: 2498 3215 3877 4231 2498
Size: 717 662 354 5010
Fragments arranged by size:
5010 717 662 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2194 4326 4459 4918 5127 2194
Size: 2132 133 459 209 3810
Fragments arranged by size:
3810 2132 459 209 133
TatI w'GTAC_w
Cuts at: 443 819 1138 1682 1816 3519 6258 443
Size: 376 319 544 134 1703 2739 928
Fragments arranged by size:
2739 1703 928 544 376 319 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 1711 1743 2833 2865 6260 6292 524
Size: 32 1155 32 1090 32 3395 32 975
Fragments arranged by size:
3395 1155 1090 975 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1756 2319 4540 1756
Size: 563 2221 3959
Fragments arranged by size:
3959 2221 563
XbaI T'CTAG_A
Cuts at: 336 3774 336
Size: 3438 3305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3577 4238 3577
Size: 661 6082
XhoI C'TCGA_G
Cuts at: 2472 2472
Size: 6743
XmaI C'CCGG_G
Cuts at: 133 3763 133
Size: 3630 3113
XmnI GAAnn'nnTTC
Cuts at: 881 3306 3419 881
Size: 2425 113 4205
Fragments arranged by size:
4205 2425 113
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BbvCI BfrBI BglI BmgBI BmtI BsiWI BssHII BstXI
BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PshAI PsiI
PspOMI PsrI SanDI SfiI SgrAI SnaBI SrfI SwaI
ZraI