Restriction map for
pF1KA1795
(Circular) (Six-base) MAPSORT of: pF1KA1795.seq Check: 3186 from: 1 to: 4412
pF1KA1795 4412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1063 1450 1063
Size: 387 4025
Acc65I G'GTAC_C
Cuts at: 495 1428 495
Size: 933 3479
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2669 3022 3684 2237
Size: 432 353 662 2965
Fragments arranged by size:
2965 662 432 353
AflIII A'CryG_T
Cuts at: 1764 3564 3845 1764
Size: 1800 281 2331
Fragments arranged by size:
2331 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1054 1717 3155 1054
Size: 663 1438 2311
Fragments arranged by size:
2311 1438 663
ApaLI G'TGCA_C
Cuts at: 746 3250 746
Size: 2504 1908
ApoI r'AATT_y
Cuts at: 1416 3765 4021 1416
Size: 2349 256 1807
Fragments arranged by size:
2349 1807 256
AseI AT'TA_AT
Cuts at: 20 1315 20
Size: 1295 3117
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 1432 1432
Size: 4412
BamHI G'GATC_C
Cuts at: 1437 1437
Size: 4412
BanI G'GyrC_C
Cuts at: 495 1428 1842 2090 2125 495
Size: 933 414 248 35 2782
Fragments arranged by size:
2782 933 414 248 35
BanII G_rGCy'C
Cuts at: 1048 1426 2456 1048
Size: 378 1030 3004
Fragments arranged by size:
3004 1030 378
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbvCI CC'TCA_GC
Cuts at: 718 718
Size: 4412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4378
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3366 4412 2310
Size: 1056 1046 2310
Fragments arranged by size:
2310 1056 1046
BclI T'GATC_A
Cuts at: 918 1932 918
Size: 1014 3398
BglI GCCn_nnn'nGGC
Cuts at: 691 691
Size: 4412
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 265 1055 1524 265
Size: 790 469 3153
Fragments arranged by size:
3153 790 469
Bme1580I G_kGCm'C
Cuts at: 750 1282 2037 2130 3254 750
Size: 532 755 93 1124 1908
Fragments arranged by size:
1908 1124 755 532 93
BmrI ACTGGGnnnn_n'
Cuts at: 1624 1705 2040 4184 1624
Size: 81 335 2144 1852
Fragments arranged by size:
2144 1852 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 425 2779 425
Size: 2354 2058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 395 405 1189 1273 203
Size: 192 10 784 84 3342
Fragments arranged by size:
3342 784 192 84 10
Bpu10I CC'TnA_GC
Cuts at: 400 718 400
Size: 318 4094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 1588 2467 3181 3479 413
Size: 1175 879 714 298 1346
Fragments arranged by size:
1346 1175 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 444 1097 4116 444
Size: 653 3019 740
Fragments arranged by size:
3019 740 653
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1601 2122 2800 3211 3358 3632 1601
Size: 521 678 411 147 274 2381
Fragments arranged by size:
2381 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 216 246 1096 1126 216
Size: 30 850 30 3502
Fragments arranged by size:
3502 850 30 30
BseYI C'CCAG_C
Cuts at: 172 454 1275 1837 2595 3260 3668 172
Size: 282 821 562 758 665 408 916
Fragments arranged by size:
916 821 758 665 562 408 282
BsiEI CG_ry'CG
Cuts at: 104 1954 2000 3230 104
Size: 1850 46 1230 1286
Fragments arranged by size:
1850 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 266 750 1426 2204 2394 3254 266
Size: 484 676 778 190 860 1424
Fragments arranged by size:
1424 860 778 676 484 190
Bsp1286I G_dGCh'C
Cuts at: 266 750 1048 1282 1426 2037 2130 2204 2394
Size: 484 298 234 144 611 93 74 190
Cuts at: 2394 2456 3254 266
Size: 62 798 1424
Fragments arranged by size:
1424 798 611 484 298 234 190 144
93 74 62
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1462 1676 1978 2359 1462
Size: 214 302 381 3515
Fragments arranged by size:
3515 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2704 2758 4242 4407 50
Size: 2654 54 1484 165 55
Fragments arranged by size:
2654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2324 2324
Size: 4412
BsrFI r'CCGG_y
Cuts at: 2410 2591 2410
Size: 181 4231
BssHII G'CGCG_C
Cuts at: 965 965
Size: 4412
BssSI C'ACGA_G
Cuts at: 2683 3391 2683
Size: 708 3704
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1789 1384
Size: 405 4007
BstBI TT'CG_AA
Cuts at: 2773 2773
Size: 4412
BstYI r'GATC_y
Cuts at: 1 891 1359 1437 1904 1927 2262 2508 2814
Size: 890 468 78 467 23 335 246 306
Cuts at: 2814 2826 2912 3964 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 890 468 467 449 335 306 246
86 78 23 12
BtgI C'CryG_G
Cuts at: 236 236
Size: 4412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 1655 1655
Size: 4412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1166 1201 1166
Size: 35 4377
DraI TTT'AAA
Cuts at: 866 1411 866
Size: 545 3867
DraIII CAC_nnn'GTG
Cuts at: 1384 1384
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 310 2118 3462 310
Size: 1808 1344 1260
Fragments arranged by size:
1808 1344 1260
EaeI y'GGCC_r
Cuts at: 1997 2171 2562 2589 3934 1997
Size: 174 391 27 1345 2475
Fragments arranged by size:
2475 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2620 3362 3508 4222 2620
Size: 742 146 714 2810
Fragments arranged by size:
2810 742 714 146
EcoICRI GAG'CTC
Cuts at: 1424 1424
Size: 4412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 395 405 1189 1273 2237 2669 3022 3684
Size: 192 10 784 84 964 432 353 662
Cuts at: 3684 203
Size: 931
Fragments arranged by size:
964 931 784 662 432 353 192 84
10
EcoO109I rG'GnC_Cy
Cuts at: 168 1551 168
Size: 1383 3029
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4412
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1846 2094 3324 4095 1846
Size: 248 1230 771 2163
Fragments arranged by size:
2163 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 1096 1128 2254 2284 2286 2316 277
Size: 32 787 32 1126 30 2 30 2373
Fragments arranged by size:
2373 1126 787 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
HindIII A'AGCT_T
Cuts at: 507 507
Size: 4412
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 499 1432 499
Size: 933 3479
MfeI C'AATT_G
Cuts at: 610 610
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 147 1304 3171 3355 147
Size: 1157 1867 184 1204
Fragments arranged by size:
1867 1204 1157 184
MscI TGG'CCA
Cuts at: 2173 2173
Size: 4412
MslI CAynn'nnrTG
Cuts at: 2401 2528 2401
Size: 127 4285
MspA1I CmG'CkG
Cuts at: 622 1523 1837 2197 2981 3226 622
Size: 901 314 360 784 245 1808
Fragments arranged by size:
1808 901 784 360 314 245
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NspI r_CATG'y
Cuts at: 1020 1465 1965 3568 1020
Size: 445 500 1603 1864
Fragments arranged by size:
1864 1603 500 445
PasI CC'CwG_GG
Cuts at: 1365 1365
Size: 4412
PciI A'CATG_T
Cuts at: 3564 3564
Size: 4412
PflMI CCAn_nnn'nTGG
Cuts at: 385 385
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 4380
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 4412
PstI C_TGCA'G
Cuts at: 581 1459 2144 581
Size: 878 685 2849
Fragments arranged by size:
2849 878 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 622 1837 2197 622
Size: 1215 360 2837
Fragments arranged by size:
2837 1215 360
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 1426 1426
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 426 3929 426
Size: 3503 909
SfcI C'TryA_G
Cuts at: 33 577 857 930 1455 2140 3108 3299 33
Size: 544 280 73 525 685 968 191 1146
Fragments arranged by size:
1146 968 685 544 525 280 191 73
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 1434 1434
Size: 4412
SmlI C'TyrA_G
Cuts at: 428 1567 2482 3196 3458 428
Size: 1139 915 714 262 1382
Fragments arranged by size:
1382 1139 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
SspI AAT'ATT
Cuts at: 671 671
Size: 4412
StyI C'CwwG_G
Cuts at: 630 1546 1900 630
Size: 916 354 3142
Fragments arranged by size:
3142 916 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 351 424 923 1151 1246 1256 3927 351
Size: 73 499 228 95 10 2671 836
Fragments arranged by size:
2671 836 499 228 95 73 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 216 248 3929 3961 216
Size: 32 3681 32 667
Fragments arranged by size:
3681 667 32 32
Tth111I GACn'n_nGTC
Cuts at: 2209 2209
Size: 4412
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1907 1907
Size: 4412
XmaI C'CCGG_G
Cuts at: 1432 1432
Size: 4412
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BfrBI BmgBI
BmtI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI