Restriction map for
pF1KA1803
(Circular) (Six-base) MAPSORT of: pF1KA1803.seq Check: 5703 from: 1 to: 7349
pF1KA1803 7349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3757 4149 3757
Size: 392 6957
AccI GT'mk_AC
Cuts at: 939 1184 4387 939
Size: 245 3203 3901
Fragments arranged by size:
3901 3203 245
Acc65I G'GTAC_C
Cuts at: 4365 4365
Size: 7349
AclI AA'CG_TT
Cuts at: 7189 7189
Size: 7349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1538 3139 3251 3407 4025 5174 5606 6353
Size: 534 1601 112 156 618 1149 432 747
Cuts at: 6353 6621 1004
Size: 268 1732
Fragments arranged by size:
1732 1601 1149 747 618 534 432 268
156 112
AflIII A'CryG_T
Cuts at: 4119 4701 5805 6782 4119
Size: 582 1104 977 4686
Fragments arranged by size:
4686 1104 977 582
AgeI A'CCGG_T
Cuts at: 1373 5737 1373
Size: 4364 2985
AleI CACnn'nnGTG
Cuts at: 1519 1519
Size: 7349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2468 2468
Size: 7349
AlwNI CAG_nnn'CTG
Cuts at: 761 919 2647 2841 4654 6221 761
Size: 158 1728 194 1813 1567 1889
Fragments arranged by size:
1889 1813 1728 1567 194 158
ApaI G_GGCC'C
Cuts at: 131 3577 131
Size: 3446 3903
ApaLI G'TGCA_C
Cuts at: 1056 1288 2569 6119 1056
Size: 232 1281 3550 2286
Fragments arranged by size:
3550 2286 1281 232
ApoI r'AATT_y
Cuts at: 1453 2392 2554 3406 3415 4075 4353 6702 6958
Size: 939 162 852 9 660 278 2349 256
Cuts at: 6958 1453
Size: 1844
Fragments arranged by size:
2349 1844 939 852 660 278 256 162
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 7349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7349
AvaI C'yCGr_G
Cuts at: 1498 1504 3565 4369 1498
Size: 6 2061 804 4478
Fragments arranged by size:
4478 2061 804 6
BamHI G'GATC_C
Cuts at: 4374 4374
Size: 7349
BanI G'GyrC_C
Cuts at: 1177 1366 1625 2511 2719 4365 4779 5027 5062
Size: 189 259 886 208 1646 414 248 35
Cuts at: 5062 1177
Size: 3464
Fragments arranged by size:
3464 1646 886 414 259 248 208 189
35
BanII G_rGCy'C
Cuts at: 131 614 1505 1528 1571 1707 3272 3577 3958
Size: 483 891 23 43 136 1565 305 381
Cuts at: 3958 4105 4363 5393 131
Size: 147 258 1030 2087
Fragments arranged by size:
2087 1565 1030 891 483 381 305 258
147 136 43 23
BbeI G_GCGC'C
Cuts at: 1370 2723 4783 5031 1370
Size: 1353 2060 248 3688
Fragments arranged by size:
3688 2060 1353 248
BbsI GAAGACnn'nnnn_
Cuts at: 3248 4013 3248
Size: 765 6584
BbvCI CC'TCA_GC
Cuts at: 1277 1904 1277
Size: 627 6722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 332 366 1792 1826 2573 2607 332
Size: 34 1426 34 747 34 5074
Fragments arranged by size:
5074 1426 747 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2788 5247 6008 7349 2788
Size: 2459 761 1341 2788
Fragments arranged by size:
2788 2459 1341 761
BclI T'GATC_A
Cuts at: 1871 4869 1871
Size: 2998 4351
BfrBI ATG'CAT
Cuts at: 2235 2235
Size: 7349
BglII A'GATC_T
Cuts at: 3582 4864 6901 3582
Size: 1282 2037 4030
Fragments arranged by size:
4030 2037 1282
BlpI GC'TnA_GC
Cuts at: 1909 4461 1909
Size: 2552 4797
Bme1580I G_kGCm'C
Cuts at: 131 1060 1292 1743 2573 3554 3577 4974 5067
Size: 929 232 451 830 981 23 1397 93
Cuts at: 5067 6123 131
Size: 1056 1357
Fragments arranged by size:
1397 1357 1056 981 929 830 451 232
93 23
BmgBI CAC'GTC
Cuts at: 530 1329 2613 530
Size: 799 1284 5266
Fragments arranged by size:
5266 1284 799
BmrI ACTGGGnnnn_n'
Cuts at: 1464 4561 4642 4977 7121 1464
Size: 3097 81 335 2144 1692
Fragments arranged by size:
3097 2144 1692 335 81
BmtI G_CTAG'C
Cuts at: 1018 1018
Size: 7349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2382 5716 2382
Size: 3334 4015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1824 3738 3754 3942 4339 427
Size: 1397 1914 16 188 397 3437
Fragments arranged by size:
3437 1914 1397 397 188 16
Bpu10I CC'TnA_GC
Cuts at: 1277 1904 2156 1277
Size: 627 252 6470
Fragments arranged by size:
6470 627 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3118 4525 5404 5896 6194 3118
Size: 1407 879 492 298 4273
Fragments arranged by size:
4273 1407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1611 3826 7053 1611
Size: 2215 3227 1907
Fragments arranged by size:
3227 2215 1907
BsaAI yAC'GTr
Cuts at: 3540 4702 3540
Size: 1162 6187
BsaBI GATnn'nnATC
Cuts at: 6 4863 6900 6
Size: 4857 2037 455
Fragments arranged by size:
4857 2037 455
BsaHI Gr'CG_yC
Cuts at: 1367 2711 2720 3447 4780 5028 5730 7226 1367
Size: 1344 9 727 1333 248 702 1496 1490
Fragments arranged by size:
1496 1490 1344 1333 727 702 248 9
BsaWI w'CCGG_w
Cuts at: 248 1373 1622 4538 5059 5737 6011 6158 6569
Size: 1125 249 2916 521 678 274 147 411
Cuts at: 6569 248
Size: 1028
Fragments arranged by size:
2916 1125 1028 678 521 411 274 249
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1558 1588 1591 1621 1633 1663 3193 3223 1558
Size: 30 3 30 12 30 1530 30 5684
Fragments arranged by size:
5684 1530 30 30 30 30 12 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1348 1564 1567 1579 2128 2436 2871 3175 3180
Size: 216 3 12 549 308 435 304 5
Cuts at: 3180 3198 3411 3583 1348
Size: 18 213 172 5114
Fragments arranged by size:
5114 549 435 308 304 216 213 172
18 12 5 3
BseYI C'CCAG_C
Cuts at: 694 1706 2631 3547 4774 5532 6109 6605 694
Size: 1012 925 916 1227 758 577 496 1438
Fragments arranged by size:
1438 1227 1012 925 916 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3634 3634
Size: 7349
BsiEI CG_ry'CG
Cuts at: 104 357 2069 2788 4891 4937 6145 104
Size: 253 1712 719 2103 46 1208 1308
Fragments arranged by size:
2103 1712 1308 1208 719 253 46
BsiHKAI G_wGCw'C
Cuts at: 435 454 1060 1292 1951 1960 2573 3091 3958
Size: 19 606 232 659 9 613 518 867
Cuts at: 3958 4363 5141 5331 6123 435
Size: 405 778 190 792 1661
Fragments arranged by size:
1661 867 792 778 659 613 606 518
405 232 190 19 9
BsmI GAATG_Cn'
Cuts at: 811 4329 811
Size: 3518 3831
BsmBI CGTCTCn'nnnn_
Cuts at: 1330 1330
Size: 7349
Bsp1286I G_dGCh'C
Cuts at: 131 435 454 614 1060 1292 1505 1528 1571
Size: 304 19 160 446 232 213 23 43
Cuts at: 1571 1707 1743 1951 1960 2573 3091 3272 3554
Size: 136 36 208 9 613 518 181 282
Cuts at: 3554 3577 3958 4105 4363 4974 5067 5141 5331
Size: 23 381 147 258 611 93 74 190
Cuts at: 5331 5393 6123 131
Size: 62 730 1357
Fragments arranged by size:
1357 730 613 611 518 446 381 304
282 258 232 213 208 190 181 160
147 136 93 74 62 43 36 23
23 19 9
BspEI T'CCGG_A
Cuts at: 6569 6569
Size: 7349
BspHI T'CATG_A
Cuts at: 2850 6525 2850
Size: 3675 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3757 4149 4399 4613 4915 5296 3757
Size: 392 250 214 302 381 5810
Fragments arranged by size:
5810 392 381 302 250 214
BsrBI CCG'CTC
Cuts at: 50 411 5641 5695 7179 7344 50
Size: 361 5230 54 1484 165 55
Fragments arranged by size:
5230 1484 361 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1197 2631 2799 5261 1197
Size: 1434 168 2462 3285
Fragments arranged by size:
3285 2462 1434 168
BsrFI r'CCGG_y
Cuts at: 1373 3026 3686 5347 5528 5737 1373
Size: 1653 660 1661 181 209 2985
Fragments arranged by size:
2985 1661 1653 660 209 181
BssSI C'ACGA_G
Cuts at: 1653 5620 5978 1653
Size: 3967 358 3024
Fragments arranged by size:
3967 3024 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1064 1199 2192 2461 2841 2999 3497 4726 1064
Size: 135 993 269 380 158 498 1229 3687
Fragments arranged by size:
3687 1229 993 498 380 269 158 135
BstBI TT'CG_AA
Cuts at: 342 770 5710 342
Size: 428 4940 1981
Fragments arranged by size:
4940 1981 428
BstEII G'GTnAC_C
Cuts at: 1269 1269
Size: 7349
BstXI CCAn_nnnn'nTGG
Cuts at: 2049 2103 2049
Size: 54 7295
BstYI r'GATC_y
Cuts at: 1 1346 3582 4374 4841 4864 5199 5445 6457
Size: 1345 2236 792 467 23 335 246 1012
Cuts at: 6457 6543 6555 6901 1
Size: 86 12 346 449
Fragments arranged by size:
2236 1345 1012 792 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 940 940
Size: 7349
BtgI C'CryG_G
Cuts at: 1224 2967 3141 3169 4035 1224
Size: 1743 174 28 866 4538
Fragments arranged by size:
4538 1743 866 174 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 555 3980 5299 5480 5643 555
Size: 3425 1319 181 163 2261
Fragments arranged by size:
3425 2261 1319 181 163
BtsI GCAGTG_nn'
Cuts at: 454 579 1824 1937 2213 2748 3399 4592 454
Size: 125 1245 113 276 535 651 1193 3211
Fragments arranged by size:
3211 1245 1193 651 535 276 125 113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3450 3485 3450
Size: 35 7314
DraI TTT'AAA
Cuts at: 3519 4348 3519
Size: 829 6520
DraIII CAC_nnn'GTG
Cuts at: 1617 3645 1617
Size: 2028 5321
DrdI GACnn_nn'nnGTC
Cuts at: 5055 5913 5055
Size: 858 6491
EaeI y'GGCC_r
Cuts at: 145 332 2066 2785 2873 4934 5108 5499 5526
Size: 187 1734 719 88 2061 174 391 27
Cuts at: 5526 6871 145
Size: 1345 623
Fragments arranged by size:
2061 1734 1345 719 623 391 187 174
88 27
EagI C'GGCC_G
Cuts at: 2066 2785 4934 2066
Size: 719 2149 4481
Fragments arranged by size:
4481 2149 719
EarI CTCTTCn'nnn_
Cuts at: 899 1735 2105 2501 3653 5372 5582 899
Size: 836 370 396 1152 1719 210 2666
Fragments arranged by size:
2666 1719 1152 836 396 370 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 5557 5867 6013 7159 2677
Size: 2880 310 146 1146 2867
Fragments arranged by size:
2880 2867 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3956 4361 3956
Size: 405 6944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1004 1538 1824 3139 3251 3407 3738 3754
Size: 577 534 286 1315 112 156 331 16
Cuts at: 3754 3942 4025 4339 5174 5606 6353 6621 427
Size: 188 83 314 835 432 747 268 1155
Fragments arranged by size:
1315 1155 835 747 577 534 432 331
314 286 268 188 156 112 83 16
EcoO109I rG'GnC_Cy
Cuts at: 127 128 916 2259 3573 3574 4488 127
Size: 1 788 1343 1314 1 914 2988
Fragments arranged by size:
2988 1343 1314 914 788 1 1
EcoRI G'AATT_C
Cuts at: 2392 3406 3415 4353 2392
Size: 1014 9 938 5388
Fragments arranged by size:
5388 1014 938 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1968 2223 2289 3412 3759 5368 1968
Size: 255 66 1123 347 1609 3949
Fragments arranged by size:
3949 1609 1123 347 255 66
FspI TGC'GCA
Cuts at: 5130 5130
Size: 7349
HaeII r_GCGC'y
Cuts at: 1370 1800 2723 4783 5031 6053 7032 1370
Size: 430 923 2060 248 1022 979 1687
Fragments arranged by size:
2060 1687 1022 979 923 430 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1590 2981 3013 3165 3197 4160 4192 5191
Size: 32 1391 32 152 32 963 32 999
Cuts at: 5191 5221 5223 5253 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 1391 999 963 152 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3915 4388 3915
Size: 473 6876
HindIII A'AGCT_T
Cuts at: 3478 3478
Size: 7349
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 7349
KasI G'GCGC_C
Cuts at: 1366 2719 4779 5027 1366
Size: 1353 2060 248 3688
Fragments arranged by size:
3688 2060 1353 248
KpnI G_GTAC'C
Cuts at: 4369 4369
Size: 7349
MluI A'CGCG_T
Cuts at: 6782 6782
Size: 7349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2750 3885 6020 6204 2750
Size: 1135 2135 184 3895
Fragments arranged by size:
3895 2135 1135 184
MscI TGG'CCA
Cuts at: 2875 5110 2875
Size: 2235 5114
MslI CAynn'nnrTG
Cuts at: 438 673 1053 1132 1267 1519 1694 2101 2988
Size: 235 380 79 135 252 175 407 887
Cuts at: 2988 3059 5338 5465 438
Size: 71 2279 127 2322
Fragments arranged by size:
2322 2279 887 407 380 252 235 175
135 127 79 71
MspA1I CmG'CkG
Cuts at: 168 380 1276 1908 2183 2635 2644 2903 3117
Size: 212 896 632 275 452 9 259 214
Cuts at: 3117 3310 3728 3927 4460 4774 5134 6147 6392
Size: 193 418 199 533 314 360 1013 245
Cuts at: 6392 168
Size: 1125
Fragments arranged by size:
1125 1013 896 632 533 452 418 360
314 275 259 245 214 212 199 193
9
NaeI GCC'GGC
Cuts at: 3028 5530 3028
Size: 2502 4847
NarI GG'CG_CC
Cuts at: 1367 2720 4780 5028 1367
Size: 1353 2060 248 3688
Fragments arranged by size:
3688 2060 1353 248
NdeI CA'TA_TG
Cuts at: 2954 2954
Size: 7349
NgoMIV G'CCGG_C
Cuts at: 3026 5528 3026
Size: 2502 4847
NheI G'CTAG_C
Cuts at: 1014 1014
Size: 7349
NsiI A_TGCA'T
Cuts at: 2237 2237
Size: 7349
NspI r_CATG'y
Cuts at: 2235 3070 4123 4402 4902 5809 2235
Size: 835 1053 279 500 907 3775
Fragments arranged by size:
3775 1053 907 835 500 279
PasI CC'CwG_GG
Cuts at: 821 2014 821
Size: 1193 6156
PciI A'CATG_T
Cuts at: 4119 5805 4119
Size: 1686 5663
PfoI T'CCnGG_A
Cuts at: 953 1840 2684 4316 953
Size: 887 844 1632 3986
Fragments arranged by size:
3986 1632 887 844
PmeI GTTT'AAAC
Cuts at: 4348 4348
Size: 7349
PmlI CAC'GTG
Cuts at: 3540 3540
Size: 7349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 6488 6520 3116
Size: 32 3340 32 3945
Fragments arranged by size:
3945 3340 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2259
Size: 7349
PshAI GACnn'nnGTC
Cuts at: 1257 1257
Size: 7349
PspOMI G'GGCC_C
Cuts at: 127 3573 127
Size: 3446 3903
PspXI vC'TCGA_Gb
Cuts at: 1498 1498
Size: 7349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2299 2331 2299
Size: 32 7317
PstI C_TGCA'G
Cuts at: 1013 2220 4396 5081 1013
Size: 1207 2176 685 3281
Fragments arranged by size:
3281 2176 1207 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7349
PvuII CAG'CTG
Cuts at: 168 380 1908 2635 2644 2903 3117 3310 3927
Size: 212 1528 727 9 259 214 193 617
Cuts at: 3927 4774 5134 168
Size: 847 360 2383
Fragments arranged by size:
2383 1528 847 727 617 360 259 214
212 193 9
RsrII CG'GwC_CG
Cuts at: 5544 5544
Size: 7349
SacI G_AGCT'C
Cuts at: 3958 4363 3958
Size: 405 6944
SalI G'TCGA_C
Cuts at: 4386 4386
Size: 7349
SapI GCTCTTCn'nnn_
Cuts at: 3653 5372 5582 3653
Size: 1719 210 5420
Fragments arranged by size:
5420 1719 210
SbfI CC_TGCA'GG
Cuts at: 4396 4396
Size: 7349
ScaI AGT'ACT
Cuts at: 1642 6866 1642
Size: 5224 2125
SexAI A'CCwGG_T
Cuts at: 1601 1601
Size: 7349
SfcI C'TryA_G
Cuts at: 33 747 1009 2216 4392 5077 6070 6261 33
Size: 714 262 1207 2176 685 993 191 1121
Fragments arranged by size:
2176 1207 1121 993 714 685 262 191
SfoI GGC'GCC
Cuts at: 1368 2721 4781 5029 1368
Size: 1353 2060 248 3688
Fragments arranged by size:
3688 2060 1353 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7349
SmaI CCC'GGG
Cuts at: 4371 4371
Size: 7349
SmlI C'TyrA_G
Cuts at: 1498 3133 4504 5419 5911 6173 1498
Size: 1635 1371 915 492 262 2674
Fragments arranged by size:
2674 1635 1371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7349
SphI G_CATG'C
Cuts at: 2235 3070 4402 4902 2235
Size: 835 1332 500 4682
Fragments arranged by size:
4682 1332 835 500
StuI AGG'CCT
Cuts at: 2490 2490
Size: 7349
StyI C'CwwG_G
Cuts at: 132 1889 4483 4837 132
Size: 1757 2594 354 2644
Fragments arranged by size:
2644 2594 1757 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 371 2027 2588 4196 4932 5065 5524 5733 371
Size: 1656 561 1608 736 133 459 209 1987
Fragments arranged by size:
1987 1656 1608 736 561 459 209 133
TatI w'GTAC_w
Cuts at: 1640 2897 6864 1640
Size: 1257 3967 2125
Fragments arranged by size:
3967 2125 1257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1591 1623 2152 2184 3193 3225 6866 6898 1591
Size: 32 529 32 1009 32 3641 32 2042
Fragments arranged by size:
3641 2042 1009 529 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2818 5146 2818
Size: 2328 5021
XbaI T'CTAG_A
Cuts at: 552 4380 552
Size: 3828 3521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2721 4844 2721
Size: 2123 5226
XhoI C'TCGA_G
Cuts at: 1498 1498
Size: 7349
XmaI C'CCGG_G
Cuts at: 4369 4369
Size: 7349
XmnI GAAnn'nnTTC
Cuts at: 3481 3481
Size: 7349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NsiI NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BglI BsiWI BsrGI BssHII Bsu36I ClaI EcoNI EcoRV
FseI FspAI MfeI NcoI NotI NruI PacI PflMI
PsiI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI