Restriction map for
pF1KA1805
(Circular) (Six-base) MAPSORT of: pF1KA1805.seq Check: 7108 from: 1 to: 4814
pF1KA1805 4814 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1148 1355 1852 1148
Size: 207 497 4110
Fragments arranged by size:
4110 497 207
Acc65I G'GTAC_C
Cuts at: 1221 1830 1221
Size: 609 4205
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1254 2639 3071 3424 4086 1195
Size: 59 1385 432 353 662 1923
Fragments arranged by size:
1923 1385 662 432 353 59
AflII C'TTAA_G
Cuts at: 261 261
Size: 4814
AflIII A'CryG_T
Cuts at: 2166 3966 4247 2166
Size: 1800 281 2733
Fragments arranged by size:
2733 1800 281
AleI CACnn'nnGTG
Cuts at: 980 980
Size: 4814
AlwNI CAG_nnn'CTG
Cuts at: 1164 1218 2119 3557 1164
Size: 54 901 1438 2421
Fragments arranged by size:
2421 1438 901 54
ApaLI G'TGCA_C
Cuts at: 3652 3652
Size: 4814
ApoI r'AATT_y
Cuts at: 451 1450 1818 4167 4423 451
Size: 999 368 2349 256 842
Fragments arranged by size:
2349 999 842 368 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 1651 1834 1651
Size: 183 4631
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 1221 1830 2244 2492 2527 1221
Size: 609 414 248 35 3508
Fragments arranged by size:
3508 609 414 248 35
BanII G_rGCy'C
Cuts at: 1262 1393 1828 2858 1262
Size: 131 435 1030 3218
Fragments arranged by size:
3218 1030 435 131
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BbsI GAAGACnn'nnnn_
Cuts at: 104 446 652 1303 1426 104
Size: 342 206 651 123 3492
Fragments arranged by size:
3492 651 342 206 123
BbvCI CC'TCA_GC
Cuts at: 1499 1499
Size: 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 162 196 162
Size: 34 4780
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3768 4814 2712
Size: 1056 1046 2712
Fragments arranged by size:
2712 1056 1046
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 2439 2532 3656 2439
Size: 93 1124 3597
Fragments arranged by size:
3597 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 201 859 2026 2107 2442 4586 201
Size: 658 1167 81 335 2144 429
Fragments arranged by size:
2144 1167 658 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 897 1185 3181 897
Size: 288 1996 2530
Fragments arranged by size:
2530 1996 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 867 867
Size: 4814
Bpu10I CC'TnA_GC
Cuts at: 859 1499 859
Size: 640 4174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2869 3583 3881 1990
Size: 879 714 298 2923
Fragments arranged by size:
2923 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1619 4518 1619
Size: 2899 1915
BsaAI yAC'GTr
Cuts at: 2167 2167
Size: 4814
BsaBI GATnn'nnATC
Cuts at: 6 2328 4365 6
Size: 2322 2037 455
Fragments arranged by size:
2322 2037 455
BsaHI Gr'CG_yC
Cuts at: 2245 2493 3195 4691 2245
Size: 248 702 1496 2368
Fragments arranged by size:
2368 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2003 2524 3202 3613 3760 4034 2003
Size: 521 678 411 147 274 2783
Fragments arranged by size:
2783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 568 598 1471 1501 568
Size: 30 873 30 3881
Fragments arranged by size:
3881 873 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 589 1704 183
Size: 406 1115 3293
Fragments arranged by size:
3293 1115 406
BseYI C'CCAG_C
Cuts at: 2239 2997 3662 4070 2239
Size: 758 665 408 2983
Fragments arranged by size:
2983 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2356 2402 3632 104
Size: 2252 46 1230 1286
Fragments arranged by size:
2252 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 175 214 1828 2606 2796 3656 175
Size: 39 1614 778 190 860 1333
Fragments arranged by size:
1614 1333 860 778 190 39
BsmBI CGTCTCn'nnnn_
Cuts at: 1119 1119
Size: 4814
Bsp1286I G_dGCh'C
Cuts at: 175 214 1262 1393 1828 2439 2532 2606 2796
Size: 39 1048 131 435 611 93 74 190
Cuts at: 2796 2858 3656 175
Size: 62 798 1333
Fragments arranged by size:
1333 1048 798 611 435 190 131 93
74 62 39
BspHI T'CATG_A
Cuts at: 249 3246 249
Size: 2997 1817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 634 1864 2078 2380 2761 634
Size: 1230 214 302 381 2687
Fragments arranged by size:
2687 1230 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3106 3160 4644 4809 50
Size: 3056 54 1484 165 55
Fragments arranged by size:
3056 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 586 2726 586
Size: 2140 2674
BsrFI r'CCGG_y
Cuts at: 2812 2993 2812
Size: 181 4633
BsrGI T'GTAC_A
Cuts at: 1396 1396
Size: 4814
BssSI C'ACGA_G
Cuts at: 174 3085 3793 174
Size: 2911 708 1195
Fragments arranged by size:
2911 1195 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2191
Size: 4814
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 903 903
Size: 4814
BstYI r'GATC_y
Cuts at: 1 1839 2306 2329 2664 2910 3216 3228 3314
Size: 1838 467 23 335 246 306 12 86
Cuts at: 3314 4366 1
Size: 1052 449
Fragments arranged by size:
1838 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1149 1149
Size: 4814
Bsu36I CC'TnA_GG
Cuts at: 1024 1024
Size: 4814
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4470
Fragments arranged by size:
4470 181 163
BtsI GCAGTG_nn'
Cuts at: 615 641 1368 2057 615
Size: 26 727 689 3372
Fragments arranged by size:
3372 727 689 26
DraI TTT'AAA
Cuts at: 662 1813 662
Size: 1151 3663
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3864 2520
Size: 1344 3470
EaeI y'GGCC_r
Cuts at: 1181 2399 2573 2964 2991 4336 1181
Size: 1218 174 391 27 1345 1659
Fragments arranged by size:
1659 1345 1218 391 174 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4814
EarI CTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3764 3910 4624 3022
Size: 742 146 714 3212
Fragments arranged by size:
3212 742 714 146
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 867 1195 1254 2639 3071 3424 4086 867
Size: 328 59 1385 432 353 662 1595
Fragments arranged by size:
1595 1385 662 432 353 328 59
EcoO109I rG'GnC_Cy
Cuts at: 145 203 1215 1953 145
Size: 58 1012 738 3006
Fragments arranged by size:
3006 1012 738 58
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
EcoRV GAT'ATC
Cuts at: 910 1305 910
Size: 395 4419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 2248 2496 3726 4497 2248
Size: 248 1230 771 2565
Fragments arranged by size:
2565 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 732 764 2656 2686 2688 2718 732
Size: 32 1892 30 2 30 2828
Fragments arranged by size:
2828 1892 32 30 30 2
HincII GTy'rAC
Cuts at: 1853 1853
Size: 4814
HindIII A'AGCT_T
Cuts at: 472 1536 472
Size: 1064 3750
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1225 1834 1225
Size: 609 4205
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3573 3757 3573
Size: 184 4630
MscI TGG'CCA
Cuts at: 1183 2575 1183
Size: 1392 3422
MslI CAynn'nnrTG
Cuts at: 285 710 980 2803 2930 285
Size: 425 270 1823 127 2169
Fragments arranged by size:
2169 1823 425 270 127
MspA1I CmG'CkG
Cuts at: 364 1566 1925 2239 2599 3383 3628 364
Size: 1202 359 314 360 784 245 1550
Fragments arranged by size:
1550 1202 784 360 359 314 245
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 870 1867 2367 3970 870
Size: 997 500 1603 1714
Fragments arranged by size:
1714 1603 997 500
PciI A'CATG_T
Cuts at: 3966 3966
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 636 1190 636
Size: 554 4260
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1492 1524 3260 3292 1492
Size: 32 1736 32 3014
Fragments arranged by size:
3014 1736 32 32
PpuMI rG'GwC_Cy
Cuts at: 145 203 1215 145
Size: 58 1012 3744
Fragments arranged by size:
3744 1012 58
PstI C_TGCA'G
Cuts at: 948 1861 2546 948
Size: 913 685 3216
Fragments arranged by size:
3216 913 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 364 2239 2599 364
Size: 1875 360 2579
Fragments arranged by size:
2579 1875 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4814
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SanDI GG'GwC_CC
Cuts at: 145 145
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 1283 4331 1283
Size: 3048 1766
SexAI A'CCwGG_T
Cuts at: 1217 1217
Size: 4814
SfcI C'TryA_G
Cuts at: 33 944 1156 1356 1857 2542 3510 3701 33
Size: 911 212 200 501 685 968 191 1146
Fragments arranged by size:
1146 968 911 685 501 212 200 191
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 1836 1836
Size: 4814
SmlI C'TyrA_G
Cuts at: 261 1969 2884 3598 3860 261
Size: 1708 915 714 262 1215
Fragments arranged by size:
1708 1215 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 870 1867 2367 870
Size: 997 500 3317
Fragments arranged by size:
3317 997 500
StyI C'CwwG_G
Cuts at: 404 1184 1948 2302 404
Size: 780 764 354 2916
Fragments arranged by size:
2916 780 764 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2397 2530 2989 3198 2397
Size: 133 459 209 4013
Fragments arranged by size:
4013 459 209 133
TatI w'GTAC_w
Cuts at: 236 1281 1396 4329 236
Size: 1045 115 2933 721
Fragments arranged by size:
2933 1045 721 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1397 1429 1471 1503 4331 4363 1397
Size: 32 42 32 2828 32 1848
Fragments arranged by size:
2828 1848 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 4814
XmaI C'CCGG_G
Cuts at: 1834 1834
Size: 4814
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BspEI BssHII BstEII BtgI ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI