Restriction map for
pF1KA1824
(Circular) (Six-base) MAPSORT of: pF1KA1824.seq Check: 1547 from: 1 to: 5939
pF1KA1824 5939 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1042 1042
Size: 5939
AccI GT'mk_AC
Cuts at: 2977 2977
Size: 5939
Acc65I G'GTAC_C
Cuts at: 2386 2955 2386
Size: 569 5370
AclI AA'CG_TT
Cuts at: 5779 5779
Size: 5939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2303 3764 4196 4549 5211 2303
Size: 1461 432 353 662 3031
Fragments arranged by size:
3031 1461 662 432 353
AfeI AGC'GCT
Cuts at: 1465 1465
Size: 5939
AflIII A'CryG_T
Cuts at: 518 1022 1612 3291 5091 5372 518
Size: 504 590 1679 1800 281 1085
Fragments arranged by size:
1800 1679 1085 590 504 281
AhdI GACnn_n'nnGTC
Cuts at: 1485 1485
Size: 5939
AleI CACnn'nnGTG
Cuts at: 451 1611 451
Size: 1160 4779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1877 1877
Size: 5939
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 350
Size: 32 5907
AlwNI CAG_nnn'CTG
Cuts at: 312 852 1644 2274 2707 3244 4682 312
Size: 540 792 630 433 537 1438 1569
Fragments arranged by size:
1569 1438 792 630 540 537 433
ApaI G_GGCC'C
Cuts at: 1562 1562
Size: 5939
ApaLI G'TGCA_C
Cuts at: 4777 4777
Size: 5939
ApoI r'AATT_y
Cuts at: 286 1031 2084 2904 2943 5292 5548 286
Size: 745 1053 820 39 2349 256 677
Fragments arranged by size:
2349 1053 820 745 677 256 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 5939
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5939
AvaI C'yCGr_G
Cuts at: 1889 2959 1889
Size: 1070 4869
BamHI G'GATC_C
Cuts at: 2964 2964
Size: 5939
BanI G'GyrC_C
Cuts at: 1871 2376 2386 2955 3369 3617 3652 1871
Size: 505 10 569 414 248 35 4158
Fragments arranged by size:
4158 569 505 414 248 35 10
BanII G_rGCy'C
Cuts at: 562 647 707 1003 1192 1402 1562 2953 3983
Size: 85 60 296 189 210 160 1391 1030
Cuts at: 3983 562
Size: 2518
Fragments arranged by size:
2518 1391 1030 296 210 189 160 85
60
BbeI G_GCGC'C
Cuts at: 1875 3373 3621 1875
Size: 1498 248 4193
Fragments arranged by size:
4193 1498 248
BbsI GAAGACnn'nnnn_
Cuts at: 1346 1481 1888 1901 1949 1346
Size: 135 407 13 48 5336
Fragments arranged by size:
5336 407 135 48 13
BbvCI CC'TCA_GC
Cuts at: 2474 2474
Size: 5939
BciVI GTATCCnnnnn_n'
Cuts at: 3837 4893 5939 3837
Size: 1056 1046 3837
Fragments arranged by size:
3837 1056 1046
BclI T'GATC_A
Cuts at: 3459 3459
Size: 5939
BglI GCCn_nnn'nGGC
Cuts at: 131 224 266 2858 131
Size: 93 42 2592 3212
Fragments arranged by size:
3212 2592 93 42
BglII A'GATC_T
Cuts at: 1181 1740 3454 5491 1181
Size: 559 1714 2037 1629
Fragments arranged by size:
2037 1714 1629 559
BlpI GC'TnA_GC
Cuts at: 3051 3051
Size: 5939
Bme1580I G_kGCm'C
Cuts at: 1562 1911 3564 3657 4781 1562
Size: 349 1653 93 1124 2720
Fragments arranged by size:
2720 1653 1124 349 93
BmgBI CAC'GTC
Cuts at: 519 519
Size: 5939
BmrI ACTGGGnnnn_n'
Cuts at: 556 1898 2174 2547 3151 3232 3567 5711 556
Size: 1342 276 373 604 81 335 2144 784
Fragments arranged by size:
2144 1342 784 604 373 335 276 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 2406 2925 4306 763
Size: 1643 519 1381 2396
Fragments arranged by size:
2396 1643 1381 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 482 1206 1235 1416 2689 357
Size: 125 724 29 181 1273 3607
Fragments arranged by size:
3607 1273 724 181 125 29
Bpu10I CC'TnA_GC
Cuts at: 1467 2006 2461 2474 1467
Size: 539 455 13 4932
Fragments arranged by size:
4932 539 455 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3115 3994 4708 5006 3115
Size: 879 714 298 4048
Fragments arranged by size:
4048 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 321 1211 2143 2773 5643 321
Size: 890 932 630 2870 617
Fragments arranged by size:
2870 932 890 630 617
BsaAI yAC'GTr
Cuts at: 1613 3292 1613
Size: 1679 4260
BsaBI GATnn'nnATC
Cuts at: 6 3453 5490 6
Size: 3447 2037 455
Fragments arranged by size:
3447 2037 455
BsaHI Gr'CG_yC
Cuts at: 216 867 1872 3370 3618 4320 5816 216
Size: 651 1005 1498 248 702 1496 339
Fragments arranged by size:
1498 1496 1005 702 651 339 248
BsaWI w'CCGG_w
Cuts at: 3128 3649 4327 4738 4885 5159 3128
Size: 521 678 411 147 274 3908
Fragments arranged by size:
3908 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 694 724 1969 1999 694
Size: 30 1245 30 4634
Fragments arranged by size:
4634 1245 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 225 244 338 1723 1834 1837 1974 1977
Size: 96 19 94 1385 111 3 137 3
Cuts at: 1977 2480 2491 2584 2589 2901 129
Size: 503 11 93 5 312 3167
Fragments arranged by size:
3167 1385 503 312 137 111 96 94
93 19 11 5 3 3
BseYI C'CCAG_C
Cuts at: 164 845 1450 1765 2211 2329 2820 3364 4122
Size: 681 605 315 446 118 491 544 758
Cuts at: 4122 4787 5195 164
Size: 665 408 908
Fragments arranged by size:
908 758 681 665 605 544 491 446
408 315 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 1429
Size: 5939
BsiEI CG_ry'CG
Cuts at: 104 1230 3481 3527 4757 104
Size: 1126 2251 46 1230 1286
Fragments arranged by size:
2251 1286 1230 1126 46
BsiHKAI G_wGCw'C
Cuts at: 407 884 1402 2017 2354 2426 2953 3731 3921
Size: 477 518 615 337 72 527 778 190
Cuts at: 3921 4781 407
Size: 860 1565
Fragments arranged by size:
1565 860 778 615 527 518 477 337
190 72
BsmI GAATG_Cn'
Cuts at: 1866 1866
Size: 5939
BsmBI CGTCTCn'nnnn_
Cuts at: 207 207
Size: 5939
Bsp1286I G_dGCh'C
Cuts at: 407 562 647 707 884 1003 1192 1402 1562
Size: 155 85 60 177 119 189 210 160
Cuts at: 1562 1911 2017 2354 2426 2953 3564 3657 3731
Size: 349 106 337 72 527 611 93 74
Cuts at: 3731 3921 3983 4781 407
Size: 190 62 798 1565
Fragments arranged by size:
1565 798 611 527 349 337 210 190
189 177 160 155 119 106 93 85
74 72 62 60
BspHI T'CATG_A
Cuts at: 528 4371 528
Size: 3843 2096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1042 2282 2989 3203 3505 3886 1042
Size: 1240 707 214 302 381 3095
Fragments arranged by size:
3095 1240 707 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2010 4231 4285 5769 5934 50
Size: 1960 2221 54 1484 165 55
Fragments arranged by size:
2221 1960 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 474 1498 2258 2340 3851 474
Size: 1024 760 82 1511 2562
Fragments arranged by size:
2562 1511 1024 760 82
BsrFI r'CCGG_y
Cuts at: 3937 4118 3937
Size: 181 5758
BsrGI T'GTAC_A
Cuts at: 1019 1019
Size: 5939
BssSI C'ACGA_G
Cuts at: 4210 4918 4210
Size: 708 5231
BstAPI GCAn_nnn'nTGC
Cuts at: 3316 3316
Size: 5939
BstBI TT'CG_AA
Cuts at: 759 4300 759
Size: 3541 2398
BstEII G'GTnAC_C
Cuts at: 1569 1760 1569
Size: 191 5748
BstXI CCAn_nnnn'nTGG
Cuts at: 714 2608 714
Size: 1894 4045
BstYI r'GATC_y
Cuts at: 1 1041 1181 1740 2964 3431 3454 3789 4035
Size: 1040 140 559 1224 467 23 335 246
Cuts at: 4035 4341 4353 4439 5491 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1224 1052 1040 559 467 449 335 306
246 140 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 859 859
Size: 5939
BtgI C'CryG_G
Cuts at: 636 1756 636
Size: 1120 4819
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3889 4070 4233 3889
Size: 181 163 5595
Fragments arranged by size:
5595 181 163
BtsI GCAGTG_nn'
Cuts at: 510 3182 510
Size: 2672 3267
ClaI AT'CG_AT
Cuts at: 1104 1104
Size: 5939
DraI TTT'AAA
Cuts at: 2307 2938 2307
Size: 631 5308
DrdI GACnn_nn'nnGTC
Cuts at: 3645 4989 3645
Size: 1344 4595
EaeI y'GGCC_r
Cuts at: 258 600 716 1753 2850 3524 3698 4089 4116
Size: 342 116 1037 1097 674 174 391 27
Cuts at: 4116 5461 258
Size: 1345 736
Fragments arranged by size:
1345 1097 1037 736 674 391 342 174
116 27
EagI C'GGCC_G
Cuts at: 3524 3524
Size: 5939
EarI CTCTTCn'nnn_
Cuts at: 106 1161 1262 2306 2691 3962 4172 106
Size: 1055 101 1044 385 1271 210 1873
Fragments arranged by size:
1873 1271 1055 1044 385 210 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4147 4889 5035 5749 4147
Size: 742 146 714 4337
Fragments arranged by size:
4337 742 714 146
EcoICRI GAG'CTC
Cuts at: 1400 2951 1400
Size: 1551 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 482 1206 1235 1416 2303 2689 3764 4196
Size: 125 724 29 181 887 386 1075 432
Cuts at: 4196 4549 5211 357
Size: 353 662 1085
Fragments arranged by size:
1085 1075 887 724 662 432 386 353
181 125 29
EcoO109I rG'GnC_Cy
Cuts at: 183 249 842 1558 1559 2271 2457 2549 2805
Size: 66 593 716 1 712 186 92 256
Cuts at: 2805 3078 183
Size: 273 3044
Fragments arranged by size:
3044 716 712 593 273 256 186 92
66 1
EcoRI G'AATT_C
Cuts at: 286 1031 2904 2943 286
Size: 745 1873 39 3282
Fragments arranged by size:
3282 1873 745 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3958 3958
Size: 5939
FspI TGC'GCA
Cuts at: 3720 3720
Size: 5939
HaeII r_GCGC'y
Cuts at: 1467 1662 1875 3373 3621 4851 5622 1467
Size: 195 213 1498 248 1230 771 1784
Fragments arranged by size:
1784 1498 1230 771 248 213 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1503 1612 1644 1966 1969 1998 2001 2053
Size: 32 109 32 322 3 29 3 52
Cuts at: 2053 2085 2707 2739 3781 3811 3813 3843 1471
Size: 32 622 32 1042 30 2 30 3567
Fragments arranged by size:
3567 1042 622 322 109 52 32 32
32 32 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1074 1116 2978 1074
Size: 42 1862 4035
Fragments arranged by size:
4035 1862 42
HindIII A'AGCT_T
Cuts at: 2093 2093
Size: 5939
HpaI GTT'AAC
Cuts at: 1116 1116
Size: 5939
KasI G'GCGC_C
Cuts at: 1871 3369 3617 1871
Size: 1498 248 4193
Fragments arranged by size:
4193 1498 248
KpnI G_GTAC'C
Cuts at: 2390 2959 2390
Size: 569 5370
MluI A'CGCG_T
Cuts at: 5372 5372
Size: 5939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 317 437 1210 1464 4698 4882 317
Size: 120 773 254 3234 184 1374
Fragments arranged by size:
3234 1374 773 254 184 120
MscI TGG'CCA
Cuts at: 718 2852 3700 718
Size: 2134 848 2957
Fragments arranged by size:
2957 2134 848
MslI CAynn'nnrTG
Cuts at: 451 533 1144 1611 2251 2606 3928 4055 451
Size: 82 611 467 640 355 1322 127 2335
Fragments arranged by size:
2335 1322 640 611 467 355 127 82
MspA1I CmG'CkG
Cuts at: 168 393 1250 1857 2528 3050 3364 3724 4508
Size: 225 857 607 671 522 314 360 784
Cuts at: 4508 4753 168
Size: 245 1354
Fragments arranged by size:
1354 857 784 671 607 522 360 314
245 225
NaeI GCC'GGC
Cuts at: 4120 4120
Size: 5939
NarI GG'CG_CC
Cuts at: 1872 3370 3618 1872
Size: 1498 248 4193
Fragments arranged by size:
4193 1498 248
NcoI C'CATG_G
Cuts at: 636 636
Size: 5939
NgoMIV G'CCGG_C
Cuts at: 4118 4118
Size: 5939
NspI r_CATG'y
Cuts at: 1026 2992 3492 5095 1026
Size: 1966 500 1603 1870
Fragments arranged by size:
1966 1870 1603 500
PciI A'CATG_T
Cuts at: 1022 5091 1022
Size: 4069 1870
PflMI CCAn_nnn'nTGG
Cuts at: 312 852 312
Size: 540 5399
PfoI T'CCnGG_A
Cuts at: 1334 1334
Size: 5939
PmeI GTTT'AAAC
Cuts at: 2938 2938
Size: 5939
PmlI CAC'GTG
Cuts at: 1613 1613
Size: 5939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 5907
PpuMI rG'GwC_Cy
Cuts at: 183 249 842 2271 2549 2805 183
Size: 66 593 1429 278 256 3317
Fragments arranged by size:
3317 1429 593 278 256 66
PsiI TTA'TAA
Cuts at: 2090 2090
Size: 5939
PspOMI G'GGCC_C
Cuts at: 1558 1558
Size: 5939
PstI C_TGCA'G
Cuts at: 232 1041 2293 2986 3671 232
Size: 809 1252 693 685 2500
Fragments arranged by size:
2500 1252 809 693 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5939
PvuII CAG'CTG
Cuts at: 393 3364 3724 393
Size: 2971 360 2608
Fragments arranged by size:
2971 2608 360
RsrII CG'GwC_CG
Cuts at: 4134 4134
Size: 5939
SacI G_AGCT'C
Cuts at: 1402 2953 1402
Size: 1551 4388
SalI G'TCGA_C
Cuts at: 2976 2976
Size: 5939
SanDI GG'GwC_CC
Cuts at: 183 842 183
Size: 659 5280
SapI GCTCTTCn'nnn_
Cuts at: 1161 3962 4172 1161
Size: 2801 210 2928
Fragments arranged by size:
2928 2801 210
SbfI CC_TGCA'GG
Cuts at: 2986 2986
Size: 5939
ScaI AGT'ACT
Cuts at: 1431 5456 1431
Size: 4025 1914
SfcI C'TryA_G
Cuts at: 33 228 1037 2289 2417 2982 3667 4635 4826
Size: 195 809 1252 128 565 685 968 191
Cuts at: 4826 33
Size: 1146
Fragments arranged by size:
1252 1146 968 809 685 565 195 191
128
SfiI GGCCn_nnn'nGGCC
Cuts at: 2858 2858
Size: 5939
SfoI GGC'GCC
Cuts at: 1873 3371 3619 1873
Size: 1498 248 4193
Fragments arranged by size:
4193 1498 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5939
SmaI CCC'GGG
Cuts at: 2961 2961
Size: 5939
SmlI C'TyrA_G
Cuts at: 3094 4009 4723 4985 3094
Size: 915 714 262 4048
Fragments arranged by size:
4048 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5939
SphI G_CATG'C
Cuts at: 2992 3492 2992
Size: 500 5439
StyI C'CwwG_G
Cuts at: 636 2156 2501 3073 3427 636
Size: 1520 345 572 354 3148
Fragments arranged by size:
3148 1520 572 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 1623 1779 3522 3655 4114 4323 1320
Size: 303 156 1743 133 459 209 2936
Fragments arranged by size:
2936 1743 459 303 209 156 133
TatI w'GTAC_w
Cuts at: 1019 1210 1271 1429 5454 1019
Size: 191 61 158 4025 1504
Fragments arranged by size:
4025 1504 191 158 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5456 5488 5456
Size: 32 5907
Tth111I GACn'n_nGTC
Cuts at: 2553 3736 2553
Size: 1183 4756
XbaI T'CTAG_A
Cuts at: 2970 2970
Size: 5939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1447 3434 1447
Size: 1987 3952
XmaI C'CCGG_G
Cuts at: 2959 2959
Size: 5939
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BspEI BssHII BstZ17I CspCI DraIII EcoNI
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI