Restriction map for
pF1KA1826
(Circular) (Six-base) MAPSORT of: pF1KA1826.seq Check: 9269 from: 1 to: 4148
pF1KA1826 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 499 499
Size: 4148
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 323 1164 323
Size: 841 3307
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 841 1973 2405 3152 3420 452
Size: 389 1132 432 747 268 1180
Fragments arranged by size:
1180 1132 747 432 389 268
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 471 568 1118 1152 3501 3757 471
Size: 97 550 34 2349 256 862
Fragments arranged by size:
2349 862 550 256 97 34
AseI AT'TA_AT
Cuts at: 20 227 20
Size: 207 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 1168 1168
Size: 4148
BamHI G'GATC_C
Cuts at: 514 552 1173 514
Size: 38 621 3489
Fragments arranged by size:
3489 621 38
BanI G'GyrC_C
Cuts at: 323 1164 1578 1826 1861 323
Size: 841 414 248 35 2610
Fragments arranged by size:
2610 841 414 248 35
BanII G_rGCy'C
Cuts at: 1162 2192 1162
Size: 1030 3118
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglII A'GATC_T
Cuts at: 691 1663 3700 691
Size: 972 2037 1139
Fragments arranged by size:
2037 1139 972
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 1773 1866 2922 1773
Size: 93 1056 2999
Fragments arranged by size:
2999 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1360 1441 1776 3920 1360
Size: 81 335 2144 1588
Fragments arranged by size:
2144 1588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1324 2203 2695 2993 1027
Size: 297 879 492 298 2182
Fragments arranged by size:
2182 879 492 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 3852 3852
Size: 4148
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 396 595 598 396
Size: 199 3 3946
Fragments arranged by size:
3946 199 3
BseYI C'CCAG_C
Cuts at: 1573 2331 2908 3404 1573
Size: 758 577 496 2317
Fragments arranged by size:
2317 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 342 1162 1940 2130 2922 342
Size: 820 778 190 792 1568
Fragments arranged by size:
1568 820 792 778 190
BsmI GAATG_Cn'
Cuts at: 279 279
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 342 1162 1773 1866 1940 2130 2192 2922 342
Size: 820 611 93 74 190 62 730 1568
Fragments arranged by size:
1568 820 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 499 777 1131 1198 1412 1714 2095 499
Size: 278 354 67 214 302 381 2552
Fragments arranged by size:
2552 381 354 302 278 214 67
BsrBI CCG'CTC
Cuts at: 50 852 873 2440 2494 3978 4143 50
Size: 802 21 1567 54 1484 165 55
Fragments arranged by size:
1567 1484 802 165 55 54 21
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 1116 2509 1116
Size: 1393 2755
BstYI r'GATC_y
Cuts at: 1 514 552 691 1173 1640 1663 1998 2244
Size: 513 38 139 482 467 23 335 246
Cuts at: 2244 3256 3342 3354 3700 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 513 482 467 449 346 335 246
139 86 38 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DraIII CAC_nnn'GTG
Cuts at: 268 268
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 1854 2712 1854
Size: 858 3290
EaeI y'GGCC_r
Cuts at: 1733 1907 2298 2325 3670 1733
Size: 174 391 27 1345 2211
Fragments arranged by size:
2211 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 248 343 428 533 2171 2381 248
Size: 95 85 105 1638 210 2015
Fragments arranged by size:
2015 1638 210 105 95 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 2666 2812 3958 2356
Size: 310 146 1146 2546
Fragments arranged by size:
2546 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 841 1973 2405 3152 3420 452
Size: 389 1132 432 747 268 1180
Fragments arranged by size:
1180 1132 747 432 389 268
EcoO109I rG'GnC_Cy
Cuts at: 1287 1287
Size: 4148
EcoRI G'AATT_C
Cuts at: 1118 1152 1118
Size: 34 4114
EcoRV GAT'ATC
Cuts at: 768 768
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1107 1129 2167 1107
Size: 22 1038 3088
Fragments arranged by size:
3088 1038 22
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 2852 3831 1582
Size: 248 1022 979 1899
Fragments arranged by size:
1899 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 752 784 913 945 1990 2020 2022 2052 752
Size: 32 129 32 1045 30 2 30 2848
Fragments arranged by size:
2848 1045 129 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 327 1168 327
Size: 841 3307
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 728 2819 3003 728
Size: 2091 184 1873
Fragments arranged by size:
2091 1873 184
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 656 2137 2264 656
Size: 1481 127 2540
Fragments arranged by size:
2540 1481 127
MspA1I CmG'CkG
Cuts at: 1259 1573 1933 2946 3191 1259
Size: 314 360 1013 245 2216
Fragments arranged by size:
2216 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NdeI CA'TA_TG
Cuts at: 699 699
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 1201 1701 2608 1201
Size: 500 907 2741
Fragments arranged by size:
2741 907 500
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PfoI T'CCnGG_A
Cuts at: 619 619
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PstI C_TGCA'G
Cuts at: 788 881 1046 1076 1195 1880 788
Size: 93 165 30 119 685 3056
Fragments arranged by size:
3056 685 165 119 93 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1573 1933 1573
Size: 360 3788
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 278 784 877 1042 1072 1191 1876 2869
Size: 245 506 93 165 30 119 685 993
Cuts at: 2869 3060 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 506 245 191 165 119
93 30
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 1006 1303 2218 2710 2972 1006
Size: 297 915 492 262 2182
Fragments arranged by size:
2182 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
SspI AAT'ATT
Cuts at: 726 726
Size: 4148
StyI C'CwwG_G
Cuts at: 932 1282 1636 932
Size: 350 354 3444
Fragments arranged by size:
3444 354 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1731 1864 2323 2532 1731
Size: 133 459 209 3347
Fragments arranged by size:
3347 459 209 133
TatI w'GTAC_w
Cuts at: 3663 3663
Size: 4148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 688 1179 688
Size: 491 3657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 1643 375
Size: 1268 2880
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI