Restriction map for
pF1KA1838
(Circular) (Six-base) MAPSORT of: pF1KA1838.seq Check: 2265 from: 1 to: 5843
pF1KA1838 5843 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2044 2044
Size: 5843
AccI GT'mk_AC
Cuts at: 265 1210 1390 1426 1462 1498 1534 1994 2881
Size: 945 180 36 36 36 36 460 887
Cuts at: 2881 265
Size: 3227
Fragments arranged by size:
3227 945 887 460 180 36 36 36
36
Acc65I G'GTAC_C
Cuts at: 2859 2859
Size: 5843
AclI AA'CG_TT
Cuts at: 5683 5683
Size: 5843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 1814 2647 3668 4100 4453 5115 1385
Size: 429 833 1021 432 353 662 2113
Fragments arranged by size:
2113 1021 833 662 432 429 353
AflIII A'CryG_T
Cuts at: 1279 3195 4995 5276 1279
Size: 1916 1800 281 1846
Fragments arranged by size:
1916 1846 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1990 1990
Size: 5843
AleI CACnn'nnGTG
Cuts at: 1783 1783
Size: 5843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2655 2655
Size: 5843
AlwNI CAG_nnn'CTG
Cuts at: 1147 1183 1219 1255 1291 1327 1363 1399 1435
Size: 36 36 36 36 36 36 36 36
Cuts at: 1435 1471 1507 1543 1579 1828 2036 3148 4586
Size: 36 36 36 36 249 208 1112 1438
Cuts at: 4586 1147
Size: 2404
Fragments arranged by size:
2404 1438 1112 249 208 36 36 36
36 36 36 36 36 36 36 36
36
ApaLI G'TGCA_C
Cuts at: 4681 4681
Size: 5843
ApoI r'AATT_y
Cuts at: 171 1723 2614 2847 5196 5452 171
Size: 1552 891 233 2349 256 562
Fragments arranged by size:
2349 1552 891 562 256 233
AseI AT'TA_AT
Cuts at: 20 20
Size: 5843
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
AvaI C'yCGr_G
Cuts at: 2863 2863
Size: 5843
BamHI G'GATC_C
Cuts at: 2868 2868
Size: 5843
BanI G'GyrC_C
Cuts at: 2084 2859 3273 3521 3556 2084
Size: 775 414 248 35 4371
Fragments arranged by size:
4371 775 414 248 35
BanII G_rGCy'C
Cuts at: 695 2428 2685 2695 2728 2749 2857 3887 695
Size: 1733 257 10 33 21 108 1030 2651
Fragments arranged by size:
2651 1733 1030 257 108 33 21 10
BbeI G_GCGC'C
Cuts at: 3277 3525 3277
Size: 248 5595
BbsI GAAGACnn'nnnn_
Cuts at: 647 1670 1778 2497 2724 647
Size: 1023 108 719 227 3766
Fragments arranged by size:
3766 1023 719 227 108
BciVI GTATCCnnnnn_n'
Cuts at: 218 2075 3741 4797 5843 218
Size: 1857 1666 1056 1046 218
Fragments arranged by size:
1857 1666 1056 1046 218
BclI T'GATC_A
Cuts at: 3363 3363
Size: 5843
BglI GCCn_nnn'nGGC
Cuts at: 794 794
Size: 5843
BglII A'GATC_T
Cuts at: 2599 3358 5395 2599
Size: 759 2037 3047
Fragments arranged by size:
3047 2037 759
BlpI GC'TnA_GC
Cuts at: 2955 2955
Size: 5843
Bme1580I G_kGCm'C
Cuts at: 3468 3561 4685 3468
Size: 93 1124 4626
Fragments arranged by size:
4626 1124 93
BmgBI CAC'GTC
Cuts at: 1860 1860
Size: 5843
BmrI ACTGGGnnnn_n'
Cuts at: 559 2017 2697 3055 3136 3471 5615 559
Size: 1458 680 358 81 335 2144 787
Fragments arranged by size:
2144 1458 787 680 358 335 81
BmtI G_CTAG'C
Cuts at: 520 520
Size: 5843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 754 4210 754
Size: 3456 2387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 595 2335 2712 254
Size: 341 1740 377 3385
Fragments arranged by size:
3385 1740 377 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 2208 3019 3898 4612 4910 409
Size: 1799 811 879 714 298 1342
Fragments arranged by size:
1799 1342 879 811 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2780 5547 2780
Size: 2767 3076
BsaAI yAC'GTr
Cuts at: 1280 3196 1280
Size: 1916 3927
BsaBI GATnn'nnATC
Cuts at: 6 3357 5394 6
Size: 3351 2037 455
Fragments arranged by size:
3351 2037 455
BsaHI Gr'CG_yC
Cuts at: 1109 3274 3522 4224 5720 1109
Size: 2165 248 702 1496 1232
Fragments arranged by size:
2165 1496 1232 702 248
BsaWI w'CCGG_w
Cuts at: 3032 3553 4231 4642 4789 5063 3032
Size: 521 678 411 147 274 3812
Fragments arranged by size:
3812 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2211 2211
Size: 5843
BseYI C'CCAG_C
Cuts at: 349 1954 2421 3268 4026 4691 5099 349
Size: 1605 467 847 758 665 408 1093
Fragments arranged by size:
1605 1093 847 758 665 467 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 2437
Size: 5843
BsiEI CG_ry'CG
Cuts at: 104 3385 3431 4661 104
Size: 3281 46 1230 1286
Fragments arranged by size:
3281 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 194 695 2857 3635 3825 4685 194
Size: 501 2162 778 190 860 1352
Fragments arranged by size:
2162 1352 860 778 501 190
BsmI GAATG_Cn'
Cuts at: 1918 1918
Size: 5843
Bsp1286I G_dGCh'C
Cuts at: 194 695 2428 2685 2695 2728 2749 2857 3468
Size: 501 1733 257 10 33 21 108 611
Cuts at: 3468 3561 3635 3825 3887 4685 194
Size: 93 74 190 62 798 1352
Fragments arranged by size:
1733 1352 798 611 501 257 190 108
93 74 62 33 21 10
BspHI T'CATG_A
Cuts at: 1892 4275 1892
Size: 2383 3460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 154 2044 2893 3107 3409 3790 154
Size: 1890 849 214 302 381 2207
Fragments arranged by size:
2207 1890 849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4135 4189 5673 5838 50
Size: 4085 54 1484 165 55
Fragments arranged by size:
4085 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3755 3755
Size: 5843
BsrFI r'CCGG_y
Cuts at: 3841 4022 3841
Size: 181 5662
BsrGI T'GTAC_A
Cuts at: 160 1174 1282 1354 1603 1639 1675 1747 1883
Size: 1014 108 72 249 36 36 72 136
Cuts at: 1883 160
Size: 4120
Fragments arranged by size:
4120 1014 249 136 108 72 72 36
36
BssSI C'ACGA_G
Cuts at: 1098 4114 4822 1098
Size: 3016 708 2119
Fragments arranged by size:
3016 2119 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2648 3220 2648
Size: 572 5271
BstBI TT'CG_AA
Cuts at: 4204 4204
Size: 5843
BstXI CCAn_nnnn'nTGG
Cuts at: 278 1785 278
Size: 1507 4336
BstYI r'GATC_y
Cuts at: 1 281 1644 2599 2868 3335 3358 3693 3939
Size: 280 1363 955 269 467 23 335 246
Cuts at: 3939 4245 4257 4343 5395 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1363 1052 955 467 449 335 306 280
269 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 266 1211 1391 1427 1463 1499 1535 266
Size: 945 180 36 36 36 36 4574
Fragments arranged by size:
4574 945 180 36 36 36 36
Bsu36I CC'TnA_GG
Cuts at: 510 510
Size: 5843
BtgI C'CryG_G
Cuts at: 107 1042 1814 2439 2779 107
Size: 935 772 625 340 3171
Fragments arranged by size:
3171 935 772 625 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3793 3974 4137 3793
Size: 181 163 5499
Fragments arranged by size:
5499 181 163
BtsI GCAGTG_nn'
Cuts at: 1905 3086 1905
Size: 1181 4662
DraI TTT'AAA
Cuts at: 2842 2842
Size: 5843
DrdI GACnn_nn'nnGTC
Cuts at: 3549 4893 3549
Size: 1344 4499
EaeI y'GGCC_r
Cuts at: 291 764 2676 3428 3602 3993 4020 5365 291
Size: 473 1912 752 174 391 27 1345 769
Fragments arranged by size:
1912 1345 769 752 473 391 174 27
EagI C'GGCC_G
Cuts at: 3428 3428
Size: 5843
EarI CTCTTCn'nnn_
Cuts at: 782 956 2225 3866 4076 782
Size: 174 1269 1641 210 2549
Fragments arranged by size:
2549 1641 1269 210 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4051 4793 4939 5653 4051
Size: 742 146 714 4241
Fragments arranged by size:
4241 742 714 146
EcoICRI GAG'CTC
Cuts at: 693 2855 693
Size: 2162 3681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 595 1385 1814 2335 2647 2712 3668 4100
Size: 341 790 429 521 312 65 956 432
Cuts at: 4100 4453 5115 254
Size: 353 662 982
Fragments arranged by size:
982 956 790 662 521 432 429 353
341 312 65
EcoO109I rG'GnC_Cy
Cuts at: 1180 1288 1360 1504 1540 1789 2982 1180
Size: 108 72 144 36 249 1193 4041
Fragments arranged by size:
4041 1193 249 144 108 72 36
EcoRI G'AATT_C
Cuts at: 2847 2847
Size: 5843
EcoRV GAT'ATC
Cuts at: 443 443
Size: 5843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1954 3862 1954
Size: 1908 3935
FspI TGC'GCA
Cuts at: 2339 3624 2339
Size: 1285 4558
HaeII r_GCGC'y
Cuts at: 2187 3277 3525 4755 5526 2187
Size: 1090 248 1230 771 2504
Fragments arranged by size:
2504 1230 1090 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 904 936 957 989 1099 1131 1135 1167 1567
Size: 32 21 32 110 32 4 32 400
Cuts at: 1567 1599 1636 1668 1976 2008 2591 2623 3685
Size: 32 37 32 308 32 583 32 1062
Cuts at: 3685 3715 3717 3747 904
Size: 30 2 30 3000
Fragments arranged by size:
3000 1062 583 400 308 110 37 32
32 32 32 32 32 32 32 30
30 21 4 2
HincII GTy'rAC
Cuts at: 2466 2882 2466
Size: 416 5427
HindIII A'AGCT_T
Cuts at: 828 2261 2530 828
Size: 1433 269 4141
Fragments arranged by size:
4141 1433 269
KasI G'GCGC_C
Cuts at: 3273 3521 3273
Size: 248 5595
KpnI G_GTAC'C
Cuts at: 2863 2863
Size: 5843
MluI A'CGCG_T
Cuts at: 5276 5276
Size: 5843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4602 4786 4602
Size: 184 5659
MscI TGG'CCA
Cuts at: 293 3604 293
Size: 3311 2532
MslI CAynn'nnrTG
Cuts at: 112 156 718 1783 3832 3959 112
Size: 44 562 1065 2049 127 1996
Fragments arranged by size:
2049 1996 1065 562 127 44
MspA1I CmG'CkG
Cuts at: 349 1989 2106 2421 2441 2954 3268 3628 4412
Size: 1640 117 315 20 513 314 360 784
Cuts at: 4412 4657 349
Size: 245 1535
Fragments arranged by size:
1640 1535 784 513 360 315 314 245
117 20
NaeI GCC'GGC
Cuts at: 4024 4024
Size: 5843
NarI GG'CG_CC
Cuts at: 3274 3522 3274
Size: 248 5595
NcoI C'CATG_G
Cuts at: 107 1042 1814 107
Size: 935 772 4136
Fragments arranged by size:
4136 935 772
NdeI CA'TA_TG
Cuts at: 1636 1636
Size: 5843
NgoMIV G'CCGG_C
Cuts at: 4022 4022
Size: 5843
NheI G'CTAG_C
Cuts at: 516 516
Size: 5843
NspI r_CATG'y
Cuts at: 822 2329 2477 2896 3396 4999 822
Size: 1507 148 419 500 1603 1666
Fragments arranged by size:
1666 1603 1507 500 419 148
PciI A'CATG_T
Cuts at: 4995 4995
Size: 5843
PfoI T'CCnGG_A
Cuts at: 210 445 210
Size: 235 5608
PmeI GTTT'AAAC
Cuts at: 2842 2842
Size: 5843
PmlI CAC'GTG
Cuts at: 1280 1280
Size: 5843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 4289
Size: 32 5811
PshAI GACnn'nnGTC
Cuts at: 668 668
Size: 5843
PsiI TTA'TAA
Cuts at: 1001 1001
Size: 5843
PstI C_TGCA'G
Cuts at: 730 2111 2240 2890 3575 730
Size: 1381 129 650 685 2998
Fragments arranged by size:
2998 1381 685 650 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5843
PvuII CAG'CTG
Cuts at: 349 2421 3268 3628 349
Size: 2072 847 360 2564
Fragments arranged by size:
2564 2072 847 360
RsrII CG'GwC_CG
Cuts at: 4038 4038
Size: 5843
SacI G_AGCT'C
Cuts at: 695 2857 695
Size: 2162 3681
SacII CC_GC'GG
Cuts at: 2442 2442
Size: 5843
SalI G'TCGA_C
Cuts at: 2880 2880
Size: 5843
SapI GCTCTTCn'nnn_
Cuts at: 2225 3866 4076 2225
Size: 1641 210 3992
Fragments arranged by size:
3992 1641 210
SbfI CC_TGCA'GG
Cuts at: 2890 2890
Size: 5843
ScaI AGT'ACT
Cuts at: 2548 5360 2548
Size: 2812 3031
SexAI A'CCwGG_T
Cuts at: 1877 1877
Size: 5843
SfcI C'TryA_G
Cuts at: 33 726 1706 2107 2236 2386 2886 3571 4539
Size: 693 980 401 129 150 500 685 968
Cuts at: 4539 4730 33
Size: 191 1146
Fragments arranged by size:
1146 980 968 693 685 500 401 191
150 129
SfoI GGC'GCC
Cuts at: 3275 3523 3275
Size: 248 5595
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
SmaI CCC'GGG
Cuts at: 2865 2865
Size: 5843
SmlI C'TyrA_G
Cuts at: 424 2223 2998 3913 4627 4889 424
Size: 1799 775 915 714 262 1378
Fragments arranged by size:
1799 1378 915 775 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5843
SphI G_CATG'C
Cuts at: 2329 2896 3396 2329
Size: 567 500 4776
Fragments arranged by size:
4776 567 500
SspI AAT'ATT
Cuts at: 2147 2147
Size: 5843
StuI AGG'CCT
Cuts at: 1948 1963 2344 2647 1948
Size: 15 381 303 5144
Fragments arranged by size:
5144 381 303 15
StyI C'CwwG_G
Cuts at: 107 792 1042 1814 2355 2977 3331 107
Size: 685 250 772 541 622 354 2619
Fragments arranged by size:
2619 772 685 622 541 354 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 399 3426 3559 4018 4227 100
Size: 299 3027 133 459 209 1716
Fragments arranged by size:
3027 1716 459 299 209 133
TatI w'GTAC_w
Cuts at: 160 1174 1282 1354 1603 1639 1675 1747 1883
Size: 1014 108 72 249 36 36 72 136
Cuts at: 1883 2546 5358 160
Size: 663 2812 645
Fragments arranged by size:
2812 1014 663 645 249 136 108 72
72 36 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 273 305 550 582 1844 1876 2481 2513 5360
Size: 32 245 32 1262 32 605 32 2847
Cuts at: 5360 5392 273
Size: 32 724
Fragments arranged by size:
2847 1262 724 605 245 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 715 2095 3640 715
Size: 1380 1545 2918
Fragments arranged by size:
2918 1545 1380
XbaI T'CTAG_A
Cuts at: 2874 2874
Size: 5843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3338 3338
Size: 5843
XmaI C'CCGG_G
Cuts at: 2863 2863
Size: 5843
XmnI GAAnn'nnTTC
Cuts at: 1127 1127
Size: 5843
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI Bpu10I BsaXI BsiWI BsmBI
BspEI BssHII BstEII ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NotI NruI NsiI PacI PasI
PflMI PpuMI PspOMI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI