Restriction map for
pF1KA1839
(Circular) (Six-base) MAPSORT of: pF1KA1839.seq Check: 4744 from: 1 to: 4664
pF1KA1839 4664 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 215 215
Size: 4664
AccI GT'mk_AC
Cuts at: 1702 1702
Size: 4664
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 428 889 1010 1187 1247 2489 2921 3274
Size: 160 461 121 177 60 1242 432 353
Cuts at: 3274 3936 268
Size: 662 996
Fragments arranged by size:
1242 996 662 461 432 353 177 160
121 60
AflIII A'CryG_T
Cuts at: 1395 2016 3816 4097 1395
Size: 621 1800 281 1962
Fragments arranged by size:
1962 1800 621 281
AleI CACnn'nnGTG
Cuts at: 984 984
Size: 4664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 146 146
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 451 1969 3407 451
Size: 1518 1438 1708
Fragments arranged by size:
1708 1518 1438
ApaLI G'TGCA_C
Cuts at: 3502 3502
Size: 4664
ApoI r'AATT_y
Cuts at: 400 567 687 1072 1301 1370 1383 1668 4017
Size: 167 120 385 229 69 13 285 2349
Cuts at: 4017 4273 400
Size: 256 791
Fragments arranged by size:
2349 791 385 285 256 229 167 120
69 13
AseI AT'TA_AT
Cuts at: 20 886 20
Size: 866 3798
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 118 176 1684 118
Size: 58 1508 3098
Fragments arranged by size:
3098 1508 58
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 203 1680 2094 2342 2377 203
Size: 1477 414 248 35 2490
Fragments arranged by size:
2490 1477 414 248 35
BanII G_rGCy'C
Cuts at: 157 264 1678 2708 157
Size: 107 1414 1030 2113
Fragments arranged by size:
2113 1414 1030 107
BbeI G_GCGC'C
Cuts at: 2098 2346 2098
Size: 248 4416
BbsI GAAGACnn'nnnn_
Cuts at: 743 1250 743
Size: 507 4157
BbvCI CC'TCA_GC
Cuts at: 227 779 227
Size: 552 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3618 4664 2562
Size: 1056 1046 2562
Fragments arranged by size:
2562 1056 1046
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BfrBI ATG'CAT
Cuts at: 1177 1177
Size: 4664
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 215 1776 215
Size: 1561 3103
Bme1580I G_kGCm'C
Cuts at: 2289 2382 3506 2289
Size: 93 1124 3447
Fragments arranged by size:
3447 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1876 1957 2292 4436 1876
Size: 81 335 2144 2104
Fragments arranged by size:
2144 2104 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
Bpu10I CC'TnA_GC
Cuts at: 227 779 227
Size: 552 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1840 2719 3433 3731 562
Size: 1278 879 714 298 1495
Fragments arranged by size:
1495 1278 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4368 4368
Size: 4664
BsaAI yAC'GTr
Cuts at: 2017 2017
Size: 4664
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 2095 2343 3045 4541 2095
Size: 248 702 1496 2218
Fragments arranged by size:
2218 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3463 3610 3884 1853
Size: 521 678 411 147 274 2633
Fragments arranged by size:
2633 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 150 244 767 1246 147
Size: 3 94 523 479 3565
Fragments arranged by size:
3565 523 479 94 3
BseYI C'CCAG_C
Cuts at: 2089 2847 3512 3920 2089
Size: 758 665 408 2833
Fragments arranged by size:
2833 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 186 2206 2252 3482 104
Size: 82 2020 46 1230 1286
Fragments arranged by size:
2020 1286 1230 82 46
BsiHKAI G_wGCw'C
Cuts at: 157 1678 2456 2646 3506 157
Size: 1521 778 190 860 1315
Fragments arranged by size:
1521 1315 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 157 264 1678 2289 2382 2456 2646 2708 3506
Size: 107 1414 611 93 74 190 62 798
Cuts at: 3506 157
Size: 1315
Fragments arranged by size:
1414 1315 798 611 190 107 93 74
62
BspHI T'CATG_A
Cuts at: 1149 3096 1149
Size: 1947 2717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 1167 1714 1928 2230 2611 215
Size: 952 547 214 302 381 2268
Fragments arranged by size:
2268 952 547 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2956 3010 4494 4659 50
Size: 2906 54 1484 165 55
Fragments arranged by size:
2906 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 748 2576 748
Size: 1828 2836
BsrFI r'CCGG_y
Cuts at: 210 2662 2843 210
Size: 2452 181 2031
Fragments arranged by size:
2452 2031 181
BsrGI T'GTAC_A
Cuts at: 657 657
Size: 4664
BssSI C'ACGA_G
Cuts at: 150 2935 3643 150
Size: 2785 708 1171
Fragments arranged by size:
2785 1171 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstYI r'GATC_y
Cuts at: 1 1632 1689 2156 2179 2514 2760 3066 3078
Size: 1631 57 467 23 335 246 306 12
Cuts at: 3078 3164 4216 1
Size: 86 1052 449
Fragments arranged by size:
1631 1052 467 449 335 306 246 86
57 23 12
Bsu36I CC'TnA_GG
Cuts at: 788 1637 788
Size: 849 3815
BtgI C'CryG_G
Cuts at: 107 546 1592 107
Size: 439 1046 3179
Fragments arranged by size:
3179 1046 439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2614 2795 2958 2614
Size: 181 163 4320
Fragments arranged by size:
4320 181 163
BtsI GCAGTG_nn'
Cuts at: 1907 1907
Size: 4664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 684 719 684
Size: 35 4629
DraI TTT'AAA
Cuts at: 566 1663 566
Size: 1097 3567
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3714 2370
Size: 1344 3320
EaeI y'GGCC_r
Cuts at: 2249 2423 2814 2841 4186 2249
Size: 174 391 27 1345 2727
Fragments arranged by size:
2727 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 767 2687 2897 767
Size: 1920 210 2534
Fragments arranged by size:
2534 1920 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2872 3614 3760 4474 2872
Size: 742 146 714 3062
Fragments arranged by size:
3062 742 714 146
EcoICRI GAG'CTC
Cuts at: 155 1676 155
Size: 1521 3143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 428 889 1010 1187 1247 2489 2921 3274
Size: 160 461 121 177 60 1242 432 353
Cuts at: 3274 3936 268
Size: 662 996
Fragments arranged by size:
1242 996 662 461 432 353 177 160
121 60
EcoNI CCTnn'n_nnAGG
Cuts at: 1640 1640
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 276 663 1410 1803 276
Size: 387 747 393 3137
Fragments arranged by size:
3137 747 393 387
EcoRI G'AATT_C
Cuts at: 1072 1668 1072
Size: 596 4068
EcoRV GAT'ATC
Cuts at: 137 137
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 939 2098 2346 3576 4347 939
Size: 1159 248 1230 771 1256
Fragments arranged by size:
1256 1230 1159 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 808 840 1432 1464 2506 2536 2538 2568 808
Size: 32 592 32 1042 30 2 30 2904
Fragments arranged by size:
2904 1042 592 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1440 1703 1440
Size: 263 4401
HindIII A'AGCT_T
Cuts at: 1511 1511
Size: 4664
KasI G'GCGC_C
Cuts at: 2094 2342 2094
Size: 248 4416
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3423 3607 3423
Size: 184 4480
MscI TGG'CCA
Cuts at: 2425 2425
Size: 4664
MslI CAynn'nnrTG
Cuts at: 984 2653 2780 984
Size: 1669 127 2868
Fragments arranged by size:
2868 1669 127
MspA1I CmG'CkG
Cuts at: 1099 1461 1775 2089 2449 3233 3478 1099
Size: 362 314 314 360 784 245 2285
Fragments arranged by size:
2285 784 362 360 314 314 245
NaeI GCC'GGC
Cuts at: 212 2845 212
Size: 2633 2031
NarI GG'CG_CC
Cuts at: 2095 2343 2095
Size: 248 4416
NcoI C'CATG_G
Cuts at: 107 546 1592 107
Size: 439 1046 3179
Fragments arranged by size:
3179 1046 439
NgoMIV G'CCGG_C
Cuts at: 210 2843 210
Size: 2633 2031
NsiI A_TGCA'T
Cuts at: 1179 1179
Size: 4664
NspI r_CATG'y
Cuts at: 757 1095 1399 1717 2217 3820 757
Size: 338 304 318 500 1603 1601
Fragments arranged by size:
1603 1601 500 338 318 304
PciI A'CATG_T
Cuts at: 1395 3816 1395
Size: 2421 2243
PflMI CCAn_nnn'nTGG
Cuts at: 547 547
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 276 663 1410 276
Size: 387 747 3530
Fragments arranged by size:
3530 747 387
PstI C_TGCA'G
Cuts at: 1711 2396 1711
Size: 685 3979
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 1099 2089 2449 1099
Size: 990 360 3314
Fragments arranged by size:
3314 990 360
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 157 1678 157
Size: 1521 3143
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 253 4181 253
Size: 3928 736
SfcI C'TryA_G
Cuts at: 33 1052 1365 1707 2392 3360 3551 33
Size: 1019 313 342 685 968 191 1146
Fragments arranged by size:
1146 1019 968 685 342 313 191
SfoI GGC'GCC
Cuts at: 2096 2344 2096
Size: 248 4416
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 1686 1686
Size: 4664
SmlI C'TyrA_G
Cuts at: 577 1819 2734 3448 3710 577
Size: 1242 915 714 262 1531
Fragments arranged by size:
1531 1242 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 757 1717 2217 757
Size: 960 500 3204
Fragments arranged by size:
3204 960 500
StyI C'CwwG_G
Cuts at: 107 546 632 1592 1798 2152 107
Size: 439 86 960 206 354 2619
Fragments arranged by size:
2619 960 439 354 206 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 200 783 881 2247 2380 2839 3048 200
Size: 583 98 1366 133 459 209 1816
Fragments arranged by size:
1816 1366 583 459 209 133 98
TatI w'GTAC_w
Cuts at: 251 657 4179 251
Size: 406 3522 736
Fragments arranged by size:
3522 736 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4181 4213 4181
Size: 32 4632
Tth111I GACn'n_nGTC
Cuts at: 478 2461 478
Size: 1983 2681
XbaI T'CTAG_A
Cuts at: 1276 1306 1695 1276
Size: 30 389 4245
Fragments arranged by size:
4245 389 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1215 2159 1215
Size: 944 3720
XmaI C'CCGG_G
Cuts at: 1684 1684
Size: 4664
XmnI GAAnn'nnTTC
Cuts at: 253 253
Size: 4664
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BssHII BstEII BstXI
BstZ17I ClaI DraIII FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI