Restriction map for
pF1KA1840
(Circular) (Six-base) MAPSORT of: pF1KA1840.seq Check: 7778 from: 1 to: 10442
pF1KA1840 10442 bp
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7423 7423
Size: 10442
AccI GT'mk_AC
Cuts at: 1523 3128 3228 6081 7480 1523
Size: 1605 100 2853 1399 4485
Fragments arranged by size:
4485 2853 1605 1399 100
Acc65I G'GTAC_C
Cuts at: 6697 7458 6697
Size: 761 9681
AclI AA'CG_TT
Cuts at: 10282 10282
Size: 10442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2214 2768 2827 4275 4628 4776 4832 5098
Size: 199 554 59 1448 353 148 56 266
Cuts at: 5098 6343 6765 7321 7411 8267 8699 9446 9714
Size: 1245 422 556 90 856 432 747 268
Cuts at: 9714 2015
Size: 2743
Fragments arranged by size:
2743 1448 1245 856 747 556 554 432
422 353 268 266 199 148 90 59
56
AflII C'TTAA_G
Cuts at: 4314 6238 6904 4314
Size: 1924 666 7852
Fragments arranged by size:
7852 1924 666
AflIII A'CryG_T
Cuts at: 839 7794 8898 9875 839
Size: 6955 1104 977 1406
Fragments arranged by size:
6955 1406 1104 977
AgeI A'CCGG_T
Cuts at: 8830 8830
Size: 10442
AleI CACnn'nnGTG
Cuts at: 1361 4585 1361
Size: 3224 7218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2849 5471 2849
Size: 2622 7820
AlwNI CAG_nnn'CTG
Cuts at: 1545 2555 6250 7747 9314 1545
Size: 1010 3695 1497 1567 2673
Fragments arranged by size:
3695 2673 1567 1497 1010
ApaI G_GGCC'C
Cuts at: 355 355
Size: 10442
ApaLI G'TGCA_C
Cuts at: 3507 9212 3507
Size: 5705 4737
ApoI r'AATT_y
Cuts at: 457 740 963 1743 2286 2429 2629 2812 2921
Size: 283 223 780 543 143 200 183 109
Cuts at: 2921 3211 3699 4436 5316 5328 7154 7204 7446
Size: 290 488 737 880 12 1826 50 242
Cuts at: 7446 9795 10051 457
Size: 2349 256 848
Fragments arranged by size:
2349 1826 880 848 780 737 543 488
290 283 256 242 223 200 183 143
109 50 12
AseI AT'TA_AT
Cuts at: 20 2048 3766 5075 20
Size: 2028 1718 1309 5387
Fragments arranged by size:
5387 2028 1718 1309
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10442
AvaI C'yCGr_G
Cuts at: 236 7462 236
Size: 7226 3216
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 788 821 788
Size: 33 10409
BamHI G'GATC_C
Cuts at: 3441 6687 7467 3441
Size: 3246 780 6416
Fragments arranged by size:
6416 3246 780
BanI G'GyrC_C
Cuts at: 160 198 4543 5472 5756 6697 6881 7458 7872
Size: 38 4345 929 284 941 184 577 414
Cuts at: 7872 8120 8155 160
Size: 248 35 2447
Fragments arranged by size:
4345 2447 941 929 577 414 284 248
184 38 35
BanII G_rGCy'C
Cuts at: 355 2670 3833 5567 6213 6461 7456 8486 355
Size: 2315 1163 1734 646 248 995 1030 2311
Fragments arranged by size:
2315 2311 1734 1163 1030 995 646 248
BbeI G_GCGC'C
Cuts at: 5760 7876 8124 5760
Size: 2116 248 8078
Fragments arranged by size:
8078 2116 248
BbsI GAAGACnn'nnnn_
Cuts at: 842 2978 842
Size: 2136 8306
BbvCI CC'TCA_GC
Cuts at: 7121 7121
Size: 10442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6802 6836 6802
Size: 34 10408
BciVI GTATCCnnnnn_n'
Cuts at: 3018 5052 8340 9101 10442 3018
Size: 2034 3288 761 1341 3018
Fragments arranged by size:
3288 3018 2034 1341 761
BclI T'GATC_A
Cuts at: 1803 1812 7962 1803
Size: 9 6150 4283
Fragments arranged by size:
6150 4283 9
BfrBI ATG'CAT
Cuts at: 1284 3631 1284
Size: 2347 8095
BglI GCCn_nnn'nGGC
Cuts at: 350 5380 350
Size: 5030 5412
BglII A'GATC_T
Cuts at: 1017 2381 5128 5507 5582 7957 9994 1017
Size: 1364 2747 379 75 2375 2037 1465
Fragments arranged by size:
2747 2375 2037 1465 1364 379 75
BlpI GC'TnA_GC
Cuts at: 5921 7554 5921
Size: 1633 8809
Bme1580I G_kGCm'C
Cuts at: 163 355 3511 4349 4390 5450 5477 6011 6884
Size: 192 3156 838 41 1060 27 534 873
Cuts at: 6884 8067 8160 9216 163
Size: 1183 93 1056 1389
Fragments arranged by size:
3156 1389 1183 1060 1056 873 838 534
192 93 41 27
BmgBI CAC'GTC
Cuts at: 5469 6494 5469
Size: 1025 9417
BmrI ACTGGGnnnn_n'
Cuts at: 4291 4947 6557 7654 7735 8070 10214 4291
Size: 656 1610 1097 81 335 2144 4519
Fragments arranged by size:
4519 2144 1610 1097 656 335 81
BmtI G_CTAG'C
Cuts at: 3278 7432 3278
Size: 4154 6288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 4482 4727 5017 8809 3797
Size: 685 245 290 3792 5430
Fragments arranged by size:
5430 3792 685 290 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1189 2778 4522 4698 4846 4921 5509 5518
Size: 822 1589 1744 176 148 75 588 9
Cuts at: 5518 5997 6764 367
Size: 479 767 4045
Fragments arranged by size:
4045 1744 1589 822 767 588 479 176
148 75 9
Bpu10I CC'TnA_GC
Cuts at: 6146 7121 6146
Size: 975 9467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1583 2281 2362 3889 4678 5950 6856 7618
Size: 696 698 81 1527 789 1272 906 762
Cuts at: 7618 8497 8989 9287 887
Size: 879 492 298 2042
Fragments arranged by size:
2042 1527 1272 906 879 789 762 698
696 492 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 1468 1529 10146 1468
Size: 61 8617 1764
Fragments arranged by size:
8617 1764 61
BsaAI yAC'GTr
Cuts at: 2413 7795 2413
Size: 5382 5060
BsaBI GATnn'nnATC
Cuts at: 6 2009 7956 9993 6
Size: 2003 5947 2037 455
Fragments arranged by size:
5947 2037 2003 455
BsaHI Gr'CG_yC
Cuts at: 313 5757 7873 8121 8823 10319 313
Size: 5444 2116 248 702 1496 436
Fragments arranged by size:
5444 2116 1496 702 436 248
BsaWI w'CCGG_w
Cuts at: 6167 7631 8152 8830 9104 9251 9662 6167
Size: 1464 521 678 274 147 411 6947
Fragments arranged by size:
6947 1464 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3335 3365 3335
Size: 30 10412
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 924 2278 3503 3825 4471 5431 5506 6583 924
Size: 1354 1225 322 646 960 75 1077 4783
Fragments arranged by size:
4783 1354 1225 1077 960 646 322 75
BseYI C'CCAG_C
Cuts at: 155 1127 1256 1673 2822 3446 4260 4962 5528
Size: 972 129 417 1149 624 814 702 566
Cuts at: 5528 6971 7867 8625 9202 9698 155
Size: 1443 896 758 577 496 899
Fragments arranged by size:
1443 1149 972 899 896 814 758 702
624 577 566 496 417 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1270 1400 3975 4168 5872 680
Size: 590 130 2575 193 1704 5250
Fragments arranged by size:
5250 2575 1704 590 193 130
BsiEI CG_ry'CG
Cuts at: 104 7984 8030 9238 104
Size: 7880 46 1208 1308
Fragments arranged by size:
7880 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1188 1687 3104 3511 5393 5856 6461 7456 8234
Size: 499 1417 407 1882 463 605 995 778
Cuts at: 8234 8424 9216 1188
Size: 190 792 2414
Fragments arranged by size:
2414 1882 1417 995 792 778 605 499
463 407 190
BsmI GAATG_Cn'
Cuts at: 2986 4085 2986
Size: 1099 9343
Bsp1286I G_dGCh'C
Cuts at: 163 355 1188 1687 2670 3104 3511 3833 4349
Size: 192 833 499 983 434 407 322 516
Cuts at: 4349 4390 5393 5450 5477 5567 5856 6011 6213
Size: 41 1003 57 27 90 289 155 202
Cuts at: 6213 6461 6884 7456 8067 8160 8234 8424 8486
Size: 248 423 572 611 93 74 190 62
Cuts at: 8486 9216 163
Size: 730 1389
Fragments arranged by size:
1389 1003 983 833 730 611 572 516
499 434 423 407 322 289 248 202
192 190 155 93 90 74 62 57
41 27
BspEI T'CCGG_A
Cuts at: 6167 9662 6167
Size: 3495 6947
BspHI T'CATG_A
Cuts at: 1622 2066 9618 1622
Size: 444 7552 2446
Fragments arranged by size:
7552 2446 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 7423 7492 7706 8008 8389 7423
Size: 69 214 302 381 9476
Fragments arranged by size:
9476 381 302 214 69
BsrBI CCG'CTC
Cuts at: 50 8734 8788 10272 10437 50
Size: 8684 54 1484 165 55
Fragments arranged by size:
8684 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 517 3816 4619 6477 8354 517
Size: 3299 803 1858 1877 2605
Fragments arranged by size:
3299 2605 1877 1858 803
BsrFI r'CCGG_y
Cuts at: 145 5801 8440 8621 8830 145
Size: 5656 2639 181 209 1757
Fragments arranged by size:
5656 2639 1757 209 181
BsrGI T'GTAC_A
Cuts at: 2115 2115
Size: 10442
BssSI C'ACGA_G
Cuts at: 6262 6811 8713 9071 6262
Size: 549 1902 358 7633
Fragments arranged by size:
7633 1902 549 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3108 4385 5042 7819 3108
Size: 1277 657 2777 5731
Fragments arranged by size:
5731 2777 1277 657
BstBI TT'CG_AA
Cuts at: 2058 8803 2058
Size: 6745 3697
BstEII G'GTnAC_C
Cuts at: 1231 1231
Size: 10442
BstXI CCAn_nnnn'nTGG
Cuts at: 1647 4469 4899 5402 6117 1647
Size: 2822 430 503 715 5972
Fragments arranged by size:
5972 2822 715 503 430
BstYI r'GATC_y
Cuts at: 1 770 1017 2381 3252 3441 4662 5128 5507
Size: 769 247 1364 871 189 1221 466 379
Cuts at: 5507 5582 5883 5897 6687 7467 7934 7957 8292
Size: 75 301 14 790 780 467 23 335
Cuts at: 8292 8538 9550 9636 9648 9994 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1364 1221 1012 871 790 780 769 467
466 449 379 346 335 301 247 246
189 86 75 23 14 12
Bsu36I CC'TnA_GG
Cuts at: 1037 5862 5956 1037
Size: 4825 94 5523
Fragments arranged by size:
5523 4825 94
BtgI C'CryG_G
Cuts at: 107 164 170 1189 1629 6063 6432 107
Size: 57 6 1019 440 4434 369 4117
Fragments arranged by size:
4434 4117 1019 440 369 57 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 232 8392 8573 8736 232
Size: 8160 181 163 1938
Fragments arranged by size:
8160 1938 181 163
BtsI GCAGTG_nn'
Cuts at: 710 926 1412 1495 4499 4948 5844 7685 710
Size: 216 486 83 3004 449 896 1841 3467
Fragments arranged by size:
3467 3004 1841 896 486 449 216 83
DraI TTT'AAA
Cuts at: 1613 2198 2306 2435 7441 1613
Size: 585 108 129 5006 4614
Fragments arranged by size:
5006 4614 585 129 108
DraIII CAC_nnn'GTG
Cuts at: 1511 6374 1511
Size: 4863 5579
DrdI GACnn_nn'nnGTC
Cuts at: 8148 9006 8148
Size: 858 9584
EaeI y'GGCC_r
Cuts at: 167 1088 2936 3727 3929 5171 7029 8027 8201
Size: 921 1848 791 202 1242 1858 998 174
Cuts at: 8201 8592 8619 9964 167
Size: 391 27 1345 645
Fragments arranged by size:
1858 1848 1345 1242 998 921 791 645
391 202 174 27
EagI C'GGCC_G
Cuts at: 8027 8027
Size: 10442
EarI CTCTTCn'nnn_
Cuts at: 1598 1750 1844 3502 4831 5008 6266 7238 8465
Size: 152 94 1658 1329 177 1258 972 1227
Cuts at: 8465 8675 1598
Size: 210 3365
Fragments arranged by size:
3365 1658 1329 1258 1227 972 210 177
152 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 132 8650 8960 9106 10252 132
Size: 8518 310 146 1146 322
Fragments arranged by size:
8518 1146 322 310 146
EcoICRI GAG'CTC
Cuts at: 6459 7454 6459
Size: 995 9447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1189 2015 2214 2768 2778 2827 4275 4522
Size: 822 826 199 554 10 49 1448 247
Cuts at: 4522 4628 4698 4776 4832 4846 4921 5098 5509
Size: 106 70 78 56 14 75 177 411
Cuts at: 5509 5518 5997 6343 6764 6765 7321 7411 8267
Size: 9 479 346 421 1 556 90 856
Cuts at: 8267 8699 9446 9714 367
Size: 432 747 268 1095
Fragments arranged by size:
1448 1095 856 826 822 747 556 554
479 432 421 411 346 268 247 199
177 106 90 78 75 70 56 49
14 10 9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1028 3854 7403 1028
Size: 2826 3549 4067
Fragments arranged by size:
4067 3549 2826
EcoO109I rG'GnC_Cy
Cuts at: 351 352 1033 4506 6087 6234 6909 7395 7581
Size: 1 681 3473 1581 147 675 486 186
Cuts at: 7581 351
Size: 3212
Fragments arranged by size:
3473 3212 1581 681 675 486 186 147
1
EcoRI G'AATT_C
Cuts at: 740 7446 740
Size: 6706 3736
EcoRV GAT'ATC
Cuts at: 546 546
Size: 10442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1116 2403 4069 4202 4340 4837 6675 7192 8461
Size: 1287 1666 133 138 497 1838 517 1269
Cuts at: 8461 1116
Size: 3097
Fragments arranged by size:
3097 1838 1666 1287 1269 517 497 138
133
FspI TGC'GCA
Cuts at: 250 8223 250
Size: 7973 2469
HaeII r_GCGC'y
Cuts at: 2192 5424 5760 7876 8124 9146 10125 2192
Size: 3232 336 2116 248 1022 979 2509
Fragments arranged by size:
3232 2509 2116 1022 979 336 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1314 1346 1610 1642 1707 1739 1804 1836 2226
Size: 32 264 32 65 32 65 32 390
Cuts at: 2226 2258 3335 3367 4258 4290 5719 5751 5889
Size: 32 1077 32 891 32 1429 32 138
Cuts at: 5889 5921 6074 6106 6834 6866 8284 8314 8316
Size: 32 153 32 728 32 1418 30 2
Cuts at: 8316 8346 1314
Size: 30 3410
Fragments arranged by size:
3410 1429 1418 1077 891 728 390 264
153 138 65 65 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1065 3229 6082 7481 1065
Size: 2164 2853 1399 4026
Fragments arranged by size:
4026 2853 2164 1399
HindIII A'AGCT_T
Cuts at: 2500 4927 4969 5029 2500
Size: 2427 42 60 7913
Fragments arranged by size:
7913 2427 60 42
HpaI GTT'AAC
Cuts at: 1065 1065
Size: 10442
KasI G'GCGC_C
Cuts at: 5756 7872 8120 5756
Size: 2116 248 8078
Fragments arranged by size:
8078 2116 248
KpnI G_GTAC'C
Cuts at: 6701 7462 6701
Size: 761 9681
MfeI C'AATT_G
Cuts at: 1933 2371 1933
Size: 438 10004
MluI A'CGCG_T
Cuts at: 9875 9875
Size: 10442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 962 6389 6665 6855 6905 9113 9297 962
Size: 5427 276 190 50 2208 184 2107
Fragments arranged by size:
5427 2208 2107 276 190 184 50
MscI TGG'CCA
Cuts at: 1090 2938 3931 5173 7031 8203 1090
Size: 1848 993 1242 1858 1172 3329
Fragments arranged by size:
3329 1858 1848 1242 1172 993
MslI CAynn'nnrTG
Cuts at: 1361 1634 1667 2123 2831 4585 7288 8431 8558
Size: 273 33 456 708 1754 2703 1143 127
Cuts at: 8558 1361
Size: 3245
Fragments arranged by size:
3245 2703 1754 1143 708 456 273 127
33
MspA1I CmG'CkG
Cuts at: 166 246 1545 1731 3792 6363 6820 6888 6984
Size: 80 1299 186 2061 2571 457 68 96
Cuts at: 6984 7553 7867 8227 9240 9485 166
Size: 569 314 360 1013 245 1123
Fragments arranged by size:
2571 2061 1299 1123 1013 569 457 360
314 245 186 96 80 68
NaeI GCC'GGC
Cuts at: 147 8623 147
Size: 8476 1966
NarI GG'CG_CC
Cuts at: 5757 7873 8121 5757
Size: 2116 248 8078
Fragments arranged by size:
8078 2116 248
NcoI C'CATG_G
Cuts at: 107 170 1189 1629 6063 6432 107
Size: 63 1019 440 4434 369 4117
Fragments arranged by size:
4434 4117 1019 440 369 63
NdeI CA'TA_TG
Cuts at: 801 801
Size: 10442
NgoMIV G'CCGG_C
Cuts at: 145 8621 145
Size: 8476 1966
NheI G'CTAG_C
Cuts at: 3274 7428 3274
Size: 4154 6288
NruI TCG'CGA
Cuts at: 130 130
Size: 10442
NsiI A_TGCA'T
Cuts at: 1286 3633 1286
Size: 2347 8095
NspI r_CATG'y
Cuts at: 843 1488 1702 3869 5387 5705 6540 6789 7428
Size: 645 214 2167 1518 318 835 249 639
Cuts at: 7428 7495 7995 8902 843
Size: 67 500 907 2383
Fragments arranged by size:
2383 2167 1518 907 835 645 639 500
318 249 214 67
PasI CC'CwG_GG
Cuts at: 1479 6857 1479
Size: 5378 5064
PciI A'CATG_T
Cuts at: 839 8898 839
Size: 8059 2383
PflMI CCAn_nnn'nTGG
Cuts at: 2555 2555
Size: 10442
PfoI T'CCnGG_A
Cuts at: 1196 1332 3758 6741 1196
Size: 136 2426 2983 4897
Fragments arranged by size:
4897 2983 2426 136
PmeI GTTT'AAAC
Cuts at: 7441 7441
Size: 10442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4324 4356 9581 9613 4324
Size: 32 5225 32 5153
Fragments arranged by size:
5225 5153 32 32
PpuMI rG'GwC_Cy
Cuts at: 1033 6087 7395 1033
Size: 5054 1308 4080
Fragments arranged by size:
5054 4080 1308
PsiI TTA'TAA
Cuts at: 4802 5819 4802
Size: 1017 9425
PspOMI G'GGCC_C
Cuts at: 351 351
Size: 10442
PstI C_TGCA'G
Cuts at: 117 736 3113 4159 4226 5150 5851 7010 7489
Size: 619 2377 1046 67 924 701 1159 479
Cuts at: 7489 8174 117
Size: 685 2385
Fragments arranged by size:
2385 2377 1159 1046 924 701 685 619
479 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10442
PvuII CAG'CTG
Cuts at: 246 1545 1731 3792 6363 6820 6888 7867 8227
Size: 1299 186 2061 2571 457 68 979 360
Cuts at: 8227 246
Size: 2461
Fragments arranged by size:
2571 2461 2061 1299 979 457 360 186
68
RsrII CG'GwC_CG
Cuts at: 8637 8637
Size: 10442
SacI G_AGCT'C
Cuts at: 6461 7456 6461
Size: 995 9447
SacII CC_GC'GG
Cuts at: 167 167
Size: 10442
SalI G'TCGA_C
Cuts at: 3227 6080 7479 3227
Size: 2853 1399 6190
Fragments arranged by size:
6190 2853 1399
SapI GCTCTTCn'nnn_
Cuts at: 1750 1844 4831 8465 8675 1750
Size: 94 2987 3634 210 3517
Fragments arranged by size:
3634 3517 2987 210 94
SbfI CC_TGCA'GG
Cuts at: 7489 7489
Size: 10442
ScaI AGT'ACT
Cuts at: 668 4288 9959 668
Size: 3620 5671 1151
Fragments arranged by size:
5671 3620 1151
SexAI A'CCwGG_T
Cuts at: 3665 3665
Size: 10442
SfcI C'TryA_G
Cuts at: 33 113 504 732 1303 1520 2099 2474 3109
Size: 80 391 228 571 217 579 375 635
Cuts at: 3109 3620 3683 3995 4155 4222 4936 5097 5146
Size: 511 63 312 160 67 714 161 49
Cuts at: 5146 5847 6523 7006 7485 8170 9163 9354 33
Size: 701 676 483 479 685 993 191 1121
Fragments arranged by size:
1121 993 714 701 685 676 635 579
571 511 483 479 391 375 312 228
217 191 161 160 80 67 63 49
SfoI GGC'GCC
Cuts at: 5758 7874 8122 5758
Size: 2116 248 8078
Fragments arranged by size:
8078 2116 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10442
SgrAI Cr'CCGG_yG
Cuts at: 145 145
Size: 10442
SmaI CCC'GGG
Cuts at: 238 7464 238
Size: 7226 3216
SmlI C'TyrA_G
Cuts at: 902 1598 2260 2377 3904 4314 4657 5929 6238
Size: 696 662 117 1527 410 343 1272 309
Cuts at: 6238 6871 6904 7597 8512 9004 9266 902
Size: 633 33 693 915 492 262 2078
Fragments arranged by size:
2078 1527 1272 915 696 693 662 633
492 410 343 309 262 117 33
SpeI A'CTAG_T
Cuts at: 67 5590 67
Size: 5523 4919
SphI G_CATG'C
Cuts at: 1488 7495 7995 1488
Size: 6007 500 3935
Fragments arranged by size:
6007 3935 500
StuI AGG'CCT
Cuts at: 2282 6217 7126 2282
Size: 3935 909 5598
Fragments arranged by size:
5598 3935 909
StyI C'CwwG_G
Cuts at: 107 170 1189 1500 1629 2080 3202 5438 5477
Size: 63 1019 311 129 451 1122 2236 39
Cuts at: 5477 5834 6063 6110 6432 6947 7576 7930 107
Size: 357 229 47 322 515 629 354 2619
Fragments arranged by size:
2619 2236 1122 1019 629 515 451 357
354 322 311 229 129 63 47 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 998 4274 4438 5408 5542 5906 6212 8025 8158
Size: 3276 164 970 134 364 306 1813 133
Cuts at: 8158 8617 8826 998
Size: 459 209 2614
Fragments arranged by size:
3276 2614 1813 970 459 364 306 209
164 134 133
TatI w'GTAC_w
Cuts at: 666 1240 2115 3974 4286 9957 666
Size: 574 875 1859 312 5671 1151
Fragments arranged by size:
5671 1859 1151 875 574 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1807 1839 1940 1972 4897 4929 9959 9991 1807
Size: 32 101 32 2925 32 5030 32 2258
Fragments arranged by size:
5030 2925 2258 101 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7285 8239 7285
Size: 954 9488
XbaI T'CTAG_A
Cuts at: 7473 7473
Size: 10442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1340 4356 5536 6021 7937 1340
Size: 3016 1180 485 1916 3845
Fragments arranged by size:
3845 3016 1916 1180 485
XmaI C'CCGG_G
Cuts at: 236 7462 236
Size: 7226 3216
XmnI GAAnn'nnTTC
Cuts at: 1019 1019
Size: 10442
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BsiWI BsmBI
BssHII BstZ17I ClaI CspCI FseI FspAI NotI PacI
PmlI PshAI PspXI PsrI SanDI SfiI SnaBI SrfI
SspI SwaI XhoI ZraI