Restriction map for
pF1KA1847
(Circular) (Six-base) MAPSORT of: pF1KA1847.seq Check: 4717 from: 1 to: 4646
pF1KA1847 4646 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 153 442 696 153
Size: 289 254 4103
Fragments arranged by size:
4103 289 254
AccI GT'mk_AC
Cuts at: 1684 1684
Size: 4646
Acc65I G'GTAC_C
Cuts at: 1662 1662
Size: 4646
AclI AA'CG_TT
Cuts at: 4486 4486
Size: 4646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 658 2471 2903 3650 3918 256
Size: 402 1813 432 747 268 984
Fragments arranged by size:
1813 984 747 432 402 268
AflIII A'CryG_T
Cuts at: 1376 1998 3102 4079 1376
Size: 622 1104 977 1943
Fragments arranged by size:
1943 1104 977 622
AgeI A'CCGG_T
Cuts at: 3034 3034
Size: 4646
AleI CACnn'nnGTG
Cuts at: 826 826
Size: 4646
AlwNI CAG_nnn'CTG
Cuts at: 445 530 994 1951 3518 445
Size: 85 464 957 1567 1573
Fragments arranged by size:
1573 1567 957 464 85
ApaI G_GGCC'C
Cuts at: 1335 1410 1335
Size: 75 4571
ApaLI G'TGCA_C
Cuts at: 1039 3416 1039
Size: 2377 2269
ApoI r'AATT_y
Cuts at: 1650 3999 4255 1650
Size: 2349 256 2041
Fragments arranged by size:
2349 2041 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4646
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 318 1154 1666 318
Size: 836 512 3298
Fragments arranged by size:
3298 836 512
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 181 381 1219 1238 1662 2076 2324 2359 181
Size: 200 838 19 424 414 248 35 2468
Fragments arranged by size:
2468 838 424 414 248 200 35 19
BanII G_rGCy'C
Cuts at: 245 254 757 1133 1335 1410 1551 1578 1660
Size: 9 503 376 202 75 141 27 82
Cuts at: 1660 2690 245
Size: 1030 2201
Fragments arranged by size:
2201 1030 503 376 202 141 82 75
27 9
BbeI G_GCGC'C
Cuts at: 185 2080 2328 185
Size: 1895 248 2503
Fragments arranged by size:
2503 1895 248
BbvCI CC'TCA_GC
Cuts at: 871 871
Size: 4646
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3305 4646 2544
Size: 761 1341 2544
Fragments arranged by size:
2544 1341 761
BclI T'GATC_A
Cuts at: 2166 2166
Size: 4646
BglI GCCn_nnn'nGGC
Cuts at: 1348 1405 1348
Size: 57 4589
BglII A'GATC_T
Cuts at: 2161 4198 2161
Size: 2037 2609
BlpI GC'TnA_GC
Cuts at: 1758 1758
Size: 4646
Bme1580I G_kGCm'C
Cuts at: 538 649 1043 1335 1410 2271 2364 3420 538
Size: 111 394 292 75 861 93 1056 1764
Fragments arranged by size:
1764 1056 861 394 292 111 93 75
BmrI ACTGGGnnnn_n'
Cuts at: 1858 1939 2274 4418 1858
Size: 81 335 2144 2086
Fragments arranged by size:
2144 2086 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 606 954 3013 606
Size: 348 2059 2239
Fragments arranged by size:
2239 2059 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 562 679 948 1044 145
Size: 417 117 269 96 3747
Fragments arranged by size:
3747 417 269 117 96
Bpu10I CC'TnA_GC
Cuts at: 751 871 1411 1577 751
Size: 120 540 166 3820
Fragments arranged by size:
3820 540 166 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2701 3193 3491 1822
Size: 879 492 298 2977
Fragments arranged by size:
2977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 406 717 1177 4350 406
Size: 311 460 3173 702
Fragments arranged by size:
3173 702 460 311
BsaAI yAC'GTr
Cuts at: 1999 1999
Size: 4646
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 1516 2077 2325 3027 4523 182
Size: 1334 561 248 702 1496 305
Fragments arranged by size:
1496 1334 702 561 305 248
BsaWI w'CCGG_w
Cuts at: 403 1835 2356 3034 3308 3455 3866 403
Size: 1432 521 678 274 147 411 1183
Fragments arranged by size:
1432 1183 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 652 682 739 769 511
Size: 30 111 30 57 30 4388
Fragments arranged by size:
4388 111 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 478 1057 1268 1377 1380 1450 130
Size: 348 579 211 109 3 70 3326
Fragments arranged by size:
3326 579 348 211 109 70 3
BseYI C'CCAG_C
Cuts at: 1031 1272 1353 1454 1523 1550 1560 2071 2829
Size: 241 81 101 69 27 10 511 758
Cuts at: 2829 3406 3902 1031
Size: 577 496 1775
Fragments arranged by size:
1775 758 577 511 496 241 101 81
69 27 10
BsiEI CG_ry'CG
Cuts at: 104 2188 2234 3442 104
Size: 2084 46 1208 1308
Fragments arranged by size:
2084 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 254 757 1043 1660 2438 2628 3420 245
Size: 9 503 286 617 778 190 792 1471
Fragments arranged by size:
1471 792 778 617 503 286 190 9
Bsp1286I G_dGCh'C
Cuts at: 245 254 538 649 757 1043 1133 1335 1410
Size: 9 284 111 108 286 90 202 75
Cuts at: 1410 1551 1578 1660 2271 2364 2438 2628 2690
Size: 141 27 82 611 93 74 190 62
Cuts at: 2690 3420 245
Size: 730 1471
Fragments arranged by size:
1471 730 611 286 284 202 190 141
111 108 93 90 82 75 74 62
27 9
BspEI T'CCGG_A
Cuts at: 403 3866 403
Size: 3463 1183
BspHI T'CATG_A
Cuts at: 3822 3822
Size: 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 153 221 278 442 680 696 1018 1696 1910
Size: 68 57 164 238 16 322 678 214
Cuts at: 1910 2212 2593 153
Size: 302 381 2206
Fragments arranged by size:
2206 678 381 322 302 238 214 164
68 57 16
BsrBI CCG'CTC
Cuts at: 50 2938 2992 4476 4641 50
Size: 2888 54 1484 165 55
Fragments arranged by size:
2888 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2558 2558
Size: 4646
BsrFI r'CCGG_y
Cuts at: 184 766 1502 1585 2644 2825 3034 184
Size: 582 736 83 1059 181 209 1796
Fragments arranged by size:
1796 1059 736 582 209 181 83
BssHII G'CGCG_C
Cuts at: 512 512
Size: 4646
BssSI C'ACGA_G
Cuts at: 2917 3275 2917
Size: 358 4288
BstAPI GCAn_nnn'nTGC
Cuts at: 2023 2023
Size: 4646
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstXI CCAn_nnnn'nTGG
Cuts at: 564 981 564
Size: 417 4229
BstYI r'GATC_y
Cuts at: 1 1671 2138 2161 2496 2742 3754 3840 3852
Size: 1670 467 23 335 246 1012 86 12
Cuts at: 3852 4198 1
Size: 346 449
Fragments arranged by size:
1670 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 396 557 699 881 107
Size: 289 161 142 182 3872
Fragments arranged by size:
3872 289 182 161 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2596 2777 2940 2596
Size: 181 163 4302
Fragments arranged by size:
4302 181 163
BtsI GCAGTG_nn'
Cuts at: 910 1889 910
Size: 979 3667
DraI TTT'AAA
Cuts at: 1645 1645
Size: 4646
DrdI GACnn_nn'nnGTC
Cuts at: 2352 3210 2352
Size: 858 3788
EaeI y'GGCC_r
Cuts at: 296 782 1500 2231 2405 2796 2823 4168 296
Size: 486 718 731 174 391 27 1345 774
Fragments arranged by size:
1345 774 731 718 486 391 174 27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 277 305 464 482 569 1242 1429 2669 2879
Size: 28 159 18 87 673 187 1240 210
Cuts at: 2879 277
Size: 2044
Fragments arranged by size:
2044 1240 673 210 187 159 87 28
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 595 2854 3164 3310 4456 595
Size: 2259 310 146 1146 785
Fragments arranged by size:
2259 1146 785 310 146
EcoICRI GAG'CTC
Cuts at: 243 252 755 1658 243
Size: 9 503 903 3231
Fragments arranged by size:
3231 903 503 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 256 562 658 679 948 1044 2471 2903
Size: 111 306 96 21 269 96 1427 432
Cuts at: 2903 3650 3918 145
Size: 747 268 873
Fragments arranged by size:
1427 873 747 432 306 269 268 111
96 96 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1594 1594
Size: 4646
EcoO109I rG'GnC_Cy
Cuts at: 231 741 1007 1331 1332 1407 1785 231
Size: 510 266 324 1 75 378 3092
Fragments arranged by size:
3092 510 378 324 266 75 1
EcoRI G'AATT_C
Cuts at: 1650 1650
Size: 4646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 690 2665 690
Size: 1975 2671
FspI TGC'GCA
Cuts at: 152 916 2427 152
Size: 764 1511 2371
Fragments arranged by size:
2371 1511 764
HaeII r_GCGC'y
Cuts at: 185 318 1557 2080 2328 3350 4329 185
Size: 133 1239 523 248 1022 979 502
Fragments arranged by size:
1239 1022 979 523 502 248 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 739 771 2488 2518 2520 2550 739
Size: 32 1717 30 2 30 2835
Fragments arranged by size:
2835 1717 32 30 30 2
HincII GTy'rAC
Cuts at: 1685 1685
Size: 4646
KasI G'GCGC_C
Cuts at: 181 2076 2324 181
Size: 1895 248 2503
Fragments arranged by size:
2503 1895 248
KpnI G_GTAC'C
Cuts at: 1666 1666
Size: 4646
MluI A'CGCG_T
Cuts at: 4079 4079
Size: 4646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 959 3317 3501 959
Size: 2358 184 2104
Fragments arranged by size:
2358 2104 184
MscI TGG'CCA
Cuts at: 784 2407 784
Size: 1623 3023
MslI CAynn'nnrTG
Cuts at: 562 794 826 1075 2635 2762 562
Size: 232 32 249 1560 127 2446
Fragments arranged by size:
2446 1560 249 232 127 32
MspA1I CmG'CkG
Cuts at: 156 222 391 1357 1458 1527 1560 1757 2071
Size: 66 169 966 101 69 33 197 314
Cuts at: 2071 2431 3444 3689 156
Size: 360 1013 245 1113
Fragments arranged by size:
1113 1013 966 360 314 245 197 169
101 69 66 33
NaeI GCC'GGC
Cuts at: 186 768 1504 1587 2827 186
Size: 582 736 83 1240 2005
Fragments arranged by size:
2005 1240 736 582 83
NarI GG'CG_CC
Cuts at: 182 2077 2325 182
Size: 1895 248 2503
Fragments arranged by size:
2503 1895 248
NcoI C'CATG_G
Cuts at: 107 557 699 107
Size: 450 142 4054
Fragments arranged by size:
4054 450 142
NgoMIV G'CCGG_C
Cuts at: 184 766 1502 1585 2825 184
Size: 582 736 83 1240 2005
Fragments arranged by size:
2005 1240 736 582 83
NspI r_CATG'y
Cuts at: 1380 1699 2199 3106 1380
Size: 319 500 907 2920
Fragments arranged by size:
2920 907 500 319
PciI A'CATG_T
Cuts at: 1376 3102 1376
Size: 1726 2920
PflMI CCAn_nnn'nTGG
Cuts at: 1143 1179 1143
Size: 36 4610
PfoI T'CCnGG_A
Cuts at: 353 656 737 1475 353
Size: 303 81 738 3524
Fragments arranged by size:
3524 738 303 81
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4614
PpuMI rG'GwC_Cy
Cuts at: 231 741 1007 231
Size: 510 266 3870
Fragments arranged by size:
3870 510 266
PspOMI G'GGCC_C
Cuts at: 1331 1406 1331
Size: 75 4571
PspXI vC'TCGA_Gb
Cuts at: 1154 1154
Size: 4646
PstI C_TGCA'G
Cuts at: 161 227 691 764 1015 1193 1532 1595 1693
Size: 66 464 73 251 178 339 63 98
Cuts at: 1693 2378 161
Size: 685 2429
Fragments arranged by size:
2429 685 464 339 251 178 98 73
66 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4646
PvuII CAG'CTG
Cuts at: 156 222 1527 1560 2071 2431 156
Size: 66 1305 33 511 360 2371
Fragments arranged by size:
2371 1305 511 360 66 33
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 245 254 757 1660 245
Size: 9 503 903 3231
Fragments arranged by size:
3231 903 503 9
SalI G'TCGA_C
Cuts at: 1683 1683
Size: 4646
SapI GCTCTTCn'nnn_
Cuts at: 277 305 482 1429 2669 2879 277
Size: 28 177 947 1240 210 2044
Fragments arranged by size:
2044 1240 947 210 177 28
SbfI CC_TGCA'GG
Cuts at: 764 1693 764
Size: 929 3717
ScaI AGT'ACT
Cuts at: 4163 4163
Size: 4646
SfcI C'TryA_G
Cuts at: 33 157 223 522 687 760 1011 1189 1528
Size: 124 66 299 165 73 251 178 339
Cuts at: 1528 1591 1689 2374 3367 3558 33
Size: 63 98 685 993 191 1121
Fragments arranged by size:
1121 993 685 339 299 251 191 178
165 124 98 73 66 63
SfoI GGC'GCC
Cuts at: 183 2078 2326 183
Size: 1895 248 2503
Fragments arranged by size:
2503 1895 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SmaI CCC'GGG
Cuts at: 320 1668 320
Size: 1348 3298
SmlI C'TyrA_G
Cuts at: 1154 1801 2716 3208 3470 1154
Size: 647 915 492 262 2330
Fragments arranged by size:
2330 915 647 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4646
SphI G_CATG'C
Cuts at: 1699 2199 1699
Size: 500 4146
SrfI GCCC'GGGC
Cuts at: 320 320
Size: 4646
StuI AGG'CCT
Cuts at: 1260 1260
Size: 4646
StyI C'CwwG_G
Cuts at: 107 176 557 699 1780 2134 107
Size: 69 381 142 1081 354 2619
Fragments arranged by size:
2619 1081 381 354 142 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1633 2229 2362 2821 3030 1633
Size: 596 133 459 209 3249
Fragments arranged by size:
3249 596 459 209 133
TatI w'GTAC_w
Cuts at: 4161 4161
Size: 4646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 4163 4195 652
Size: 32 3479 32 1103
Fragments arranged by size:
3479 1103 32 32
Tth111I GACn'n_nGTC
Cuts at: 2443 2443
Size: 4646
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1140 2141 1140
Size: 1001 3645
XhoI C'TCGA_G
Cuts at: 1154 1154
Size: 4646
XmaI C'CCGG_G
Cuts at: 318 1666 318
Size: 1348 3298
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI SwaI XmnI ZraI