Restriction map for
pF1KA1849
(Circular) (Six-base) MAPSORT of: pF1KA1849.seq Check: 107 from: 1 to: 6332
pF1KA1849 6332 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1971 2507 1971
Size: 536 5796
AccI GT'mk_AC
Cuts at: 1814 3370 1814
Size: 1556 4776
Acc65I G'GTAC_C
Cuts at: 3348 3348
Size: 6332
AclI AA'CG_TT
Cuts at: 6172 6172
Size: 6332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 322 2367 4157 4589 4942 5604 178
Size: 144 2045 1790 432 353 662 906
Fragments arranged by size:
2045 1790 906 662 432 353 144
AfeI AGC'GCT
Cuts at: 2888 2888
Size: 6332
AflIII A'CryG_T
Cuts at: 3684 5484 5765 3684
Size: 1800 281 4251
Fragments arranged by size:
4251 1800 281
AleI CACnn'nnGTG
Cuts at: 893 893
Size: 6332
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1717 1749 1717
Size: 32 6300
AlwNI CAG_nnn'CTG
Cuts at: 323 1191 1314 1479 2133 2499 3123 3637 5075
Size: 868 123 165 654 366 624 514 1438
Cuts at: 5075 323
Size: 1580
Fragments arranged by size:
1580 1438 868 654 624 514 366 165
123
ApaI G_GGCC'C
Cuts at: 805 1266 2071 2229 2338 2364 805
Size: 461 805 158 109 26 4773
Fragments arranged by size:
4773 805 461 158 109 26
ApaLI G'TGCA_C
Cuts at: 1984 5170 1984
Size: 3186 3146
ApoI r'AATT_y
Cuts at: 3336 5685 5941 3336
Size: 2349 256 3727
Fragments arranged by size:
3727 2349 256
AscI GG'CGCG_CC
Cuts at: 1210 1210
Size: 6332
AseI AT'TA_AT
Cuts at: 20 20
Size: 6332
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6332
AvaI C'yCGr_G
Cuts at: 554 805 1063 1199 1258 1265 1918 1990 2374
Size: 251 258 136 59 7 653 72 384
Cuts at: 2374 2596 2640 2681 3103 3129 3352 554
Size: 222 44 41 422 26 223 3534
Fragments arranged by size:
3534 653 422 384 258 251 223 222
136 72 59 44 41 26 7
BamHI G'GATC_C
Cuts at: 3357 3357
Size: 6332
BanI G'GyrC_C
Cuts at: 247 361 1304 2627 3097 3155 3348 3762 4010
Size: 114 943 1323 470 58 193 414 248
Cuts at: 4010 4045 247
Size: 35 2534
Fragments arranged by size:
2534 1323 943 470 414 248 193 114
58 35
BanII G_rGCy'C
Cuts at: 272 724 772 805 815 1058 1148 1266 1334
Size: 452 48 33 10 243 90 118 68
Cuts at: 1334 1565 1649 2071 2229 2284 2338 2364 2724
Size: 231 84 422 158 55 54 26 360
Cuts at: 2724 2770 3110 3346 4376 272
Size: 46 340 236 1030 2228
Fragments arranged by size:
2228 1030 452 422 360 340 243 236
231 158 118 90 84 68 55 54
48 46 33 26 10
BbeI G_GCGC'C
Cuts at: 251 1308 3101 3159 3766 4014 251
Size: 1057 1793 58 607 248 2569
Fragments arranged by size:
2569 1793 1057 607 248 58
BbsI GAAGACnn'nnnn_
Cuts at: 3015 3015
Size: 6332
BbvCI CC'TCA_GC
Cuts at: 583 583
Size: 6332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 658 692 658
Size: 34 6298
BciVI GTATCCnnnnn_n'
Cuts at: 3187 4230 5286 6332 3187
Size: 1043 1056 1046 3187
Fragments arranged by size:
3187 1056 1046 1043
BclI T'GATC_A
Cuts at: 3852 3852
Size: 6332
BglI GCCn_nnn'nGGC
Cuts at: 464 2260 2765 3206 464
Size: 1796 505 441 3590
Fragments arranged by size:
3590 1796 505 441
BglII A'GATC_T
Cuts at: 3847 5884 3847
Size: 2037 4295
BlpI GC'TnA_GC
Cuts at: 202 3444 202
Size: 3242 3090
Bme1580I G_kGCm'C
Cuts at: 805 1103 1266 1465 1867 1988 2071 2229 2338
Size: 298 163 199 402 121 83 158 109
Cuts at: 2338 2364 2596 2630 2733 3957 4050 5174 805
Size: 26 232 34 103 1224 93 1124 1963
Fragments arranged by size:
1963 1224 1124 402 298 232 199 163
158 121 109 103 93 83 34 26
BmgBI CAC'GTC
Cuts at: 450 600 3285 450
Size: 150 2685 3497
Fragments arranged by size:
3497 2685 150
BmrI ACTGGGnnnn_n'
Cuts at: 766 3544 3625 3960 6104 766
Size: 2778 81 335 2144 994
Fragments arranged by size:
2778 2144 994 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 743 1584 2016 2738 4699 743
Size: 841 432 722 1961 2376
Fragments arranged by size:
2376 1961 841 722 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 351 418 927 1072 1342 1360 1540 1798
Size: 129 67 509 145 270 18 180 258
Cuts at: 1798 1879 2155 2189 2422 2653 2960 2982 3202
Size: 81 276 34 233 231 307 22 220
Cuts at: 3202 222
Size: 3352
Fragments arranged by size:
3352 509 307 276 270 258 233 231
220 180 145 129 81 67 34 22
18
Bpu10I CC'TnA_GC
Cuts at: 583 766 2077 2837 583
Size: 183 1311 760 4078
Fragments arranged by size:
4078 1311 760 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2733 3508 4387 5101 5399 1468
Size: 1265 775 879 714 298 2401
Fragments arranged by size:
2401 1265 879 775 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 751 6036 751
Size: 5285 1047
BsaAI yAC'GTr
Cuts at: 438 2613 3685 438
Size: 2175 1072 3085
Fragments arranged by size:
3085 2175 1072
BsaBI GATnn'nnATC
Cuts at: 6 3846 5883 6
Size: 3840 2037 455
Fragments arranged by size:
3840 2037 455
BsaHI Gr'CG_yC
Cuts at: 248 1305 3098 3156 3763 4011 4713 6209 248
Size: 1057 1793 58 607 248 702 1496 371
Fragments arranged by size:
1793 1496 1057 702 607 371 248 58
BsaWI w'CCGG_w
Cuts at: 2937 3521 4042 4720 5131 5278 5552 2937
Size: 584 521 678 411 147 274 3717
Fragments arranged by size:
3717 678 584 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 1120 1150 1807 1837 211
Size: 30 879 30 657 30 4706
Fragments arranged by size:
4706 879 657 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 619 716 823 1711 1774 1795 1936 2047 2980
Size: 97 107 888 63 21 141 111 933
Cuts at: 2980 619
Size: 3971
Fragments arranged by size:
3971 933 888 141 111 107 97 63
21
BseYI C'CCAG_C
Cuts at: 278 472 952 1184 2228 2283 2437 2582 2673
Size: 194 480 232 1044 55 154 145 91
Cuts at: 2673 2818 3057 3165 3757 4515 5180 5588 278
Size: 145 239 108 592 758 665 408 1022
Fragments arranged by size:
1044 1022 758 665 592 480 408 239
232 194 154 145 145 108 91 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 978 1102 1725 2181 2278 2951 309
Size: 669 124 623 456 97 673 3690
Fragments arranged by size:
3690 673 669 623 456 124 97
BsiEI CG_ry'CG
Cuts at: 104 2760 3149 3874 3920 5150 104
Size: 2656 389 725 46 1230 1286
Fragments arranged by size:
2656 1286 1230 725 389 46
BsiHKAI G_wGCw'C
Cuts at: 272 724 906 1649 1988 2177 2347 3346 4124
Size: 452 182 743 339 189 170 999 778
Cuts at: 4124 4314 5174 272
Size: 190 860 1430
Fragments arranged by size:
1430 999 860 778 743 452 339 190
189 182 170
Bsp1286I G_dGCh'C
Cuts at: 272 724 772 805 815 906 1058 1103 1148
Size: 452 48 33 10 91 152 45 45
Cuts at: 1148 1266 1334 1465 1565 1649 1867 1988 2071
Size: 118 68 131 100 84 218 121 83
Cuts at: 2071 2177 2229 2284 2338 2347 2364 2596 2630
Size: 106 52 55 54 9 17 232 34
Cuts at: 2630 2724 2733 2770 3110 3346 3957 4050 4124
Size: 94 9 37 340 236 611 93 74
Cuts at: 4124 4314 4376 5174 272
Size: 190 62 798 1430
Fragments arranged by size:
1430 798 611 452 340 236 232 218
190 152 131 121 118 106 100 94
93 91 84 83 74 68 62 55
54 52 48 45 45 37 34 33
17 10 9 9
BspEI T'CCGG_A
Cuts at: 2937 2937
Size: 6332
BspHI T'CATG_A
Cuts at: 1364 4764 1364
Size: 3400 2932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1139 1163 1971 2216 2507 3382 3596 3898 4279
Size: 24 808 245 291 875 214 302 381
Cuts at: 4279 1139
Size: 3192
Fragments arranged by size:
3192 875 808 381 302 291 245 214
24
BsrBI CCG'CTC
Cuts at: 50 402 1014 4624 4678 6162 6327 50
Size: 352 612 3610 54 1484 165 55
Fragments arranged by size:
3610 1484 612 352 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4244 4244
Size: 6332
BsrFI r'CCGG_y
Cuts at: 358 427 463 915 1004 1238 2152 2297 4330
Size: 69 36 452 89 234 914 145 2033
Cuts at: 4330 4511 358
Size: 181 2179
Fragments arranged by size:
2179 2033 914 452 234 181 145 89
69 36
BsrGI T'GTAC_A
Cuts at: 1835 1835
Size: 6332
BssHII G'CGCG_C
Cuts at: 1210 1210
Size: 6332
BssSI C'ACGA_G
Cuts at: 4603 5311 4603
Size: 708 5624
BstAPI GCAn_nnn'nTGC
Cuts at: 1479 2301 2959 3709 1479
Size: 822 658 750 4102
Fragments arranged by size:
4102 822 750 658
BstBI TT'CG_AA
Cuts at: 4693 4693
Size: 6332
BstXI CCAn_nnnn'nTGG
Cuts at: 624 1572 2475 624
Size: 948 903 4481
Fragments arranged by size:
4481 948 903
BstYI r'GATC_y
Cuts at: 1 3302 3357 3824 3847 4182 4428 4734 4746
Size: 3301 55 467 23 335 246 306 12
Cuts at: 4746 4832 5884 1
Size: 86 1052 449
Fragments arranged by size:
3301 1052 467 449 335 306 246 86
55 23 12
BtgI C'CryG_G
Cuts at: 1034 1109 1122 1586 1766 2330 2760 1034
Size: 75 13 464 180 564 430 4606
Fragments arranged by size:
4606 564 464 430 180 75 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 269 482 4282 4463 4626 269
Size: 213 3800 181 163 1975
Fragments arranged by size:
3800 1975 213 181 163
BtsI GCAGTG_nn'
Cuts at: 990 1813 2038 2577 3575 990
Size: 823 225 539 998 3747
Fragments arranged by size:
3747 998 823 539 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 719 754 719
Size: 35 6297
DraI TTT'AAA
Cuts at: 3331 3331
Size: 6332
DraIII CAC_nnn'GTG
Cuts at: 925 2613 925
Size: 1688 4644
DrdI GACnn_nn'nnGTC
Cuts at: 4038 5382 4038
Size: 1344 4988
EaeI y'GGCC_r
Cuts at: 353 429 1026 1442 1574 1751 2213 2327 2757
Size: 76 597 416 132 177 462 114 430
Cuts at: 2757 2857 3146 3917 4091 4482 4509 5854 353
Size: 100 289 771 174 391 27 1345 831
Fragments arranged by size:
1345 831 771 597 462 430 416 391
289 177 174 132 114 100 76 27
EagI C'GGCC_G
Cuts at: 2757 3146 3917 2757
Size: 389 771 5172
Fragments arranged by size:
5172 771 389
EarI CTCTTCn'nnn_
Cuts at: 583 2026 2711 4355 4565 583
Size: 1443 685 1644 210 2350
Fragments arranged by size:
2350 1644 1443 685 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 991 2500 4540 5282 5428 6142 991
Size: 1509 2040 742 146 714 1181
Fragments arranged by size:
2040 1509 1181 742 714 146
EcoICRI GAG'CTC
Cuts at: 270 722 1647 3344 270
Size: 452 925 1697 3258
Fragments arranged by size:
3258 1697 925 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 222 322 351 418 927 1072 1342 1360
Size: 44 100 29 67 509 145 270 18
Cuts at: 1360 1540 1798 1879 2155 2189 2367 2422 2653
Size: 180 258 81 276 34 178 55 231
Cuts at: 2653 2960 2982 3202 4157 4589 4942 5604 178
Size: 307 22 220 955 432 353 662 906
Fragments arranged by size:
955 906 662 509 432 353 307 276
270 258 231 220 180 178 145 100
81 67 55 44 34 29 22 18
EcoNI CCTnn'n_nnAGG
Cuts at: 868 2962 868
Size: 2094 4238
EcoO109I rG'GnC_Cy
Cuts at: 219 801 802 1128 1170 1262 2067 2068 2225
Size: 582 1 326 42 92 805 1 157
Cuts at: 2225 2335 2360 2576 2637 2664 2736 2847 3046
Size: 110 25 216 61 27 72 111 199
Cuts at: 3046 3471 219
Size: 425 3080
Fragments arranged by size:
3080 805 582 425 326 216 199 157
111 110 92 72 61 42 27 25
1 1
EcoRI G'AATT_C
Cuts at: 3336 3336
Size: 6332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 546 4351 546
Size: 3805 2527
FseI GG_CCGG'CC
Cuts at: 431 431
Size: 6332
FspI TGC'GCA
Cuts at: 4113 4113
Size: 6332
HaeII r_GCGC'y
Cuts at: 251 374 397 1308 1887 2153 2890 3101 3159
Size: 123 23 911 579 266 737 211 58
Cuts at: 3159 3766 4014 5244 6015 251
Size: 607 248 1230 771 568
Fragments arranged by size:
1230 911 771 737 607 579 568 266
248 211 123 58 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 1120 1152 1882 1914 4174 4204 4206
Size: 32 877 32 730 32 2260 30 2
Cuts at: 4206 4236 211
Size: 30 2307
Fragments arranged by size:
2307 2260 877 730 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1083 3371 1083
Size: 2288 4044
KasI G'GCGC_C
Cuts at: 247 1304 3097 3155 3762 4010 247
Size: 1057 1793 58 607 248 2569
Fragments arranged by size:
2569 1793 1057 607 248 58
KpnI G_GTAC'C
Cuts at: 3352 3352
Size: 6332
MluI A'CGCG_T
Cuts at: 5765 5765
Size: 6332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 575 3305 5091 5275 575
Size: 2730 1786 184 1632
Fragments arranged by size:
2730 1786 1632 184
MscI TGG'CCA
Cuts at: 355 1576 2215 2859 4093 355
Size: 1221 639 644 1234 2594
Fragments arranged by size:
2594 1234 1221 644 639
MslI CAynn'nnrTG
Cuts at: 893 1771 2239 4321 4448 893
Size: 878 468 2082 127 2777
Fragments arranged by size:
2777 2082 878 468 127
MspA1I CmG'CkG
Cuts at: 174 282 480 672 790 934 1025 1036 1314
Size: 108 198 192 118 144 91 11 278
Cuts at: 1314 1580 1704 1977 2292 2319 2918 3145 3443
Size: 266 124 273 315 27 599 227 298
Cuts at: 3443 3757 4117 4901 5146 174
Size: 314 360 784 245 1360
Fragments arranged by size:
1360 784 599 360 315 314 298 278
273 266 245 227 198 192 144 124
118 108 91 27 11
NaeI GCC'GGC
Cuts at: 429 465 1006 1240 2154 4513 429
Size: 36 541 234 914 2359 2248
Fragments arranged by size:
2359 2248 914 541 234 36
NarI GG'CG_CC
Cuts at: 248 1305 3098 3156 3763 4011 248
Size: 1057 1793 58 607 248 2569
Fragments arranged by size:
2569 1793 1057 607 248 58
NcoI C'CATG_G
Cuts at: 1586 1766 2330 1586
Size: 180 564 5588
Fragments arranged by size:
5588 564 180
NgoMIV G'CCGG_C
Cuts at: 427 463 1004 1238 2152 4511 427
Size: 36 541 234 914 2359 2248
Fragments arranged by size:
2359 2248 914 541 234 36
NruI TCG'CGA
Cuts at: 3274 3274
Size: 6332
NspI r_CATG'y
Cuts at: 189 1686 3385 3885 5488 189
Size: 1497 1699 500 1603 1033
Fragments arranged by size:
1699 1603 1497 1033 500
PasI CC'CwG_GG
Cuts at: 705 798 1140 2222 2660 2726 705
Size: 93 342 1082 438 66 4311
Fragments arranged by size:
4311 1082 438 342 93 66
PciI A'CATG_T
Cuts at: 5484 5484
Size: 6332
PfoI T'CCnGG_A
Cuts at: 193 536 568 1016 1049 1319 1556 1856 2032
Size: 343 32 448 33 270 237 300 176
Cuts at: 2032 2132 3179 193
Size: 100 1047 3346
Fragments arranged by size:
3346 1047 448 343 300 270 237 176
100 33 32
PmeI GTTT'AAAC
Cuts at: 3331 3331
Size: 6332
PmlI CAC'GTG
Cuts at: 2613 2613
Size: 6332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 4778
Size: 32 6300
PpuMI rG'GwC_Cy
Cuts at: 219 1128 1170 2664 2847 219
Size: 909 42 1494 183 3704
Fragments arranged by size:
3704 1494 909 183 42
PspOMI G'GGCC_C
Cuts at: 801 1262 2067 2225 2334 2360 801
Size: 461 805 158 109 26 4773
Fragments arranged by size:
4773 805 461 158 109 26
PstI C_TGCA'G
Cuts at: 671 872 1358 1982 2126 2955 3379 4064 671
Size: 201 486 624 144 829 424 685 2939
Fragments arranged by size:
2939 829 685 624 486 424 201 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6332
PvuII CAG'CTG
Cuts at: 174 282 1314 1580 1704 1977 2292 2319 3757
Size: 108 1032 266 124 273 315 27 1438
Cuts at: 3757 4117 174
Size: 360 2389
Fragments arranged by size:
2389 1438 1032 360 315 273 266 124
108 27
RsrII CG'GwC_CG
Cuts at: 4527 4527
Size: 6332
SacI G_AGCT'C
Cuts at: 272 724 1649 3346 272
Size: 452 925 1697 3258
Fragments arranged by size:
3258 1697 925 452
SacII CC_GC'GG
Cuts at: 1037 1037
Size: 6332
SalI G'TCGA_C
Cuts at: 3369 3369
Size: 6332
SanDI GG'GwC_CC
Cuts at: 2664 2847 2664
Size: 183 6149
SapI GCTCTTCn'nnn_
Cuts at: 4355 4565 4355
Size: 210 6122
SbfI CC_TGCA'GG
Cuts at: 3379 3379
Size: 6332
ScaI AGT'ACT
Cuts at: 5849 5849
Size: 6332
SfcI C'TryA_G
Cuts at: 33 667 868 876 1354 1978 2122 2951 3375
Size: 634 201 8 478 624 144 829 424
Cuts at: 3375 4060 5028 5219 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 829 685 634 624 478 424
201 191 144 8
SfoI GGC'GCC
Cuts at: 249 1306 3099 3157 3764 4012 249
Size: 1057 1793 58 607 248 2569
Fragments arranged by size:
2569 1793 1057 607 248 58
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6332
SmaI CCC'GGG
Cuts at: 1065 1201 1267 2683 3105 3131 3354 1065
Size: 136 66 1416 422 26 223 4043
Fragments arranged by size:
4043 1416 422 223 136 66 26
SmlI C'TyrA_G
Cuts at: 1483 2712 3487 4402 5116 5378 1483
Size: 1229 775 915 714 262 2437
Fragments arranged by size:
2437 1229 915 775 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6332
SphI G_CATG'C
Cuts at: 189 3385 3885 189
Size: 3196 500 2636
Fragments arranged by size:
3196 2636 500
SrfI GCCC'GGGC
Cuts at: 1201 1201
Size: 6332
StuI AGG'CCT
Cuts at: 2110 2380 2565 2110
Size: 270 185 5877
Fragments arranged by size:
5877 270 185
StyI C'CwwG_G
Cuts at: 617 1205 1586 1766 2330 3210 3466 3820 617
Size: 588 381 180 564 880 256 354 3129
Fragments arranged by size:
3129 880 588 564 381 354 256 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 1462 2235 2439 3915 4048 4507 4716 292
Size: 1170 773 204 1476 133 459 209 1908
Fragments arranged by size:
1908 1476 1170 773 459 209 204 133
TatI w'GTAC_w
Cuts at: 1652 1835 5847 1652
Size: 183 4012 2137
Fragments arranged by size:
4012 2137 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 5849 5881 2414
Size: 32 3403 32 2865
Fragments arranged by size:
3403 2865 32 32
Tth111I GACn'n_nGTC
Cuts at: 319 4129 319
Size: 3810 2522
XbaI T'CTAG_A
Cuts at: 3363 3363
Size: 6332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 889 1584 1593 1773 3827 889
Size: 695 9 180 2054 3394
Fragments arranged by size:
3394 2054 695 180 9
XmaI C'CCGG_G
Cuts at: 1063 1199 1265 2681 3103 3129 3352 1063
Size: 136 66 1416 422 26 223 4043
Fragments arranged by size:
4043 1416 422 223 136 66 26
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I ClaI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PflMI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI