Restriction map for
pF1KA1851
(Circular) (Six-base) MAPSORT of: pF1KA1851.seq Check: 8139 from: 1 to: 4625
pF1KA1851 4625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 644 668 803 644
Size: 24 135 4466
Fragments arranged by size:
4466 135 24
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 873 1641 873
Size: 768 3857
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2450 2882 3629 3897 1585
Size: 865 432 747 268 2313
Fragments arranged by size:
2313 865 747 432 268
AfeI AGC'GCT
Cuts at: 805 805
Size: 4625
AflIII A'CryG_T
Cuts at: 464 1977 3081 4058 464
Size: 1513 1104 977 1031
Fragments arranged by size:
1513 1104 1031 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 333 1525 1930 3497 333
Size: 1192 405 1567 1461
Fragments arranged by size:
1567 1461 1192 405
ApaI G_GGCC'C
Cuts at: 1178 1178
Size: 4625
ApaLI G'TGCA_C
Cuts at: 544 1219 3395 544
Size: 675 2176 1774
Fragments arranged by size:
2176 1774 675
ApoI r'AATT_y
Cuts at: 1531 1629 3978 4234 1531
Size: 98 2349 256 1922
Fragments arranged by size:
2349 1922 256 98
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 1240 1645 1240
Size: 405 4220
AvrII C'CTAG_G
Cuts at: 255 255
Size: 4625
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 204 237 865 898 204
Size: 33 628 33 3931
Fragments arranged by size:
3931 628 33 33
BamHI G'GATC_C
Cuts at: 1075 1650 1075
Size: 575 4050
BanI G'GyrC_C
Cuts at: 163 351 809 873 1068 1641 2055 2303 2338
Size: 188 458 64 195 573 414 248 35
Cuts at: 2338 163
Size: 2450
Fragments arranged by size:
2450 573 458 414 248 195 188 64
35
BanII G_rGCy'C
Cuts at: 1178 1241 1639 2669 1178
Size: 63 398 1030 3134
Fragments arranged by size:
3134 1030 398 63
BbeI G_GCGC'C
Cuts at: 355 1072 2059 2307 355
Size: 717 987 248 2673
Fragments arranged by size:
2673 987 717 248
BbsI GAAGACnn'nnnn_
Cuts at: 411 411
Size: 4625
BbvCI CC'TCA_GC
Cuts at: 1526 1526
Size: 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 268 302 382 416 594 628 268
Size: 34 80 34 178 34 4265
Fragments arranged by size:
4265 178 80 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3284 4625 2523
Size: 761 1341 2523
Fragments arranged by size:
2523 1341 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 1493 1502 1493
Size: 9 4616
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 241 583 1393 1737 241
Size: 342 810 344 3129
Fragments arranged by size:
3129 810 344 342
Bme1580I G_kGCm'C
Cuts at: 548 1178 1223 2250 2343 3399 548
Size: 630 45 1027 93 1056 1774
Fragments arranged by size:
1774 1056 1027 630 93 45
BmgBI CAC'GTC
Cuts at: 270 270
Size: 4625
BmrI ACTGGGnnnn_n'
Cuts at: 1181 1837 1918 2253 4397 1181
Size: 656 81 335 2144 1409
Fragments arranged by size:
2144 1409 656 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 942 2992 942
Size: 2050 2575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 532 1498 1571 310
Size: 222 966 73 3364
Fragments arranged by size:
3364 966 222 73
Bpu10I CC'TnA_GC
Cuts at: 310 710 823 1526 310
Size: 400 113 703 3409
Fragments arranged by size:
3409 703 400 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1801 2680 3172 3470 721
Size: 1080 879 492 298 1876
Fragments arranged by size:
1876 1080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4329 4329
Size: 4625
BsaAI yAC'GTr
Cuts at: 855 1467 1978 855
Size: 612 511 3502
Fragments arranged by size:
3502 612 511
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 352 416 1069 2056 2304 3006 4502 352
Size: 64 653 987 248 702 1496 475
Fragments arranged by size:
1496 987 702 653 475 248 64
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3287 3434 3845 1814
Size: 521 678 274 147 411 2594
Fragments arranged by size:
2594 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 664 694 664
Size: 30 4595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 669 669
Size: 4625
BseYI C'CCAG_C
Cuts at: 126 405 501 690 936 1272 1375 1438 2050
Size: 279 96 189 246 336 103 63 612
Cuts at: 2050 2808 3385 3881 126
Size: 758 577 496 870
Fragments arranged by size:
870 758 612 577 496 336 279 246
189 103 96 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 361 622 1168 1531 231
Size: 130 261 546 363 3325
Fragments arranged by size:
3325 546 363 261 130
BsiEI CG_ry'CG
Cuts at: 104 958 2167 2213 3421 104
Size: 854 1209 46 1208 1308
Fragments arranged by size:
1308 1209 1208 854 46
BsiHKAI G_wGCw'C
Cuts at: 548 968 1223 1298 1639 2417 2607 3399 548
Size: 420 255 75 341 778 190 792 1774
Fragments arranged by size:
1774 792 778 420 341 255 190 75
Bsp1286I G_dGCh'C
Cuts at: 548 968 1178 1223 1241 1298 1639 2250 2343
Size: 420 210 45 18 57 341 611 93
Cuts at: 2343 2417 2607 2669 3399 548
Size: 74 190 62 730 1774
Fragments arranged by size:
1774 730 611 420 341 210 190 93
74 62 57 45 18
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 245 294 644 668 803 1675 1889 2191 2572
Size: 49 350 24 135 872 214 302 381
Cuts at: 2572 245
Size: 2298
Fragments arranged by size:
2298 872 381 350 302 214 135 49
24
BsrBI CCG'CTC
Cuts at: 50 954 1383 2917 2971 4455 4620 50
Size: 904 429 1534 54 1484 165 55
Fragments arranged by size:
1534 1484 904 429 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1031 2537 1031
Size: 1506 3119
BsrFI r'CCGG_y
Cuts at: 686 726 2623 2804 3013 686
Size: 40 1897 181 209 2298
Fragments arranged by size:
2298 1897 209 181 40
BssHII G'CGCG_C
Cuts at: 369 576 578 844 369
Size: 207 2 266 4150
Fragments arranged by size:
4150 266 207 2
BssSI C'ACGA_G
Cuts at: 1201 1297 2896 3254 1201
Size: 96 1599 358 2572
Fragments arranged by size:
2572 1599 358 96
BstAPI GCAn_nnn'nTGC
Cuts at: 270 2002 270
Size: 1732 2893
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 690 1272 690
Size: 582 4043
BstYI r'GATC_y
Cuts at: 1 1075 1650 2117 2140 2475 2721 3733 3819
Size: 1074 575 467 23 335 246 1012 86
Cuts at: 3819 3831 4177 1
Size: 12 346 449
Fragments arranged by size:
1074 1012 575 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 439 994 439
Size: 555 4070
BtgI C'CryG_G
Cuts at: 567 1283 567
Size: 716 3909
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1077 1097 2575 2756 2919 1077
Size: 20 1478 181 163 2783
Fragments arranged by size:
2783 1478 181 163 20
BtsI GCAGTG_nn'
Cuts at: 133 1267 1868 133
Size: 1134 601 2890
Fragments arranged by size:
2890 1134 601
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 480 716 955 1022 1103 1118 1166 1503 2210
Size: 236 239 67 81 15 48 337 707
Cuts at: 2210 2384 2775 2802 4147 480
Size: 174 391 27 1345 958
Fragments arranged by size:
1345 958 707 391 337 239 236 174
81 67 48 27 15
EagI C'GGCC_G
Cuts at: 955 2210 955
Size: 1255 3370
EarI CTCTTCn'nnn_
Cuts at: 364 956 1159 2648 2858 364
Size: 592 203 1489 210 2131
Fragments arranged by size:
2131 1489 592 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1572 2833 3143 3289 4435 1572
Size: 1261 310 146 1146 1762
Fragments arranged by size:
1762 1261 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1637 1637
Size: 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 532 1498 1571 1585 2450 2882 3629 3897
Size: 222 966 73 14 865 432 747 268
Cuts at: 3897 310
Size: 1038
Fragments arranged by size:
1038 966 865 747 432 268 222 73
14
EcoO109I rG'GnC_Cy
Cuts at: 819 1175 1494 1606 1764 819
Size: 356 319 112 158 3680
Fragments arranged by size:
3680 356 319 158 112
EcoRI G'AATT_C
Cuts at: 1531 1629 1531
Size: 98 4527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 1030 2406 1030
Size: 1376 3249
FspAI rTGC'GCAy
Cuts at: 1030 1030
Size: 4625
HaeII r_GCGC'y
Cuts at: 333 355 478 511 653 807 830 1072 2059
Size: 22 123 33 142 154 23 242 987
Cuts at: 2059 2307 3329 4308 333
Size: 248 1022 979 650
Fragments arranged by size:
1022 987 979 650 248 242 154 142
123 33 23 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 664 696 2467 2497 2499 2529 442
Size: 32 190 32 1771 30 2 30 2538
Fragments arranged by size:
2538 1771 190 32 32 30 30 2
HincII GTy'rAC
Cuts at: 426 1664 426
Size: 1238 3387
KasI G'GCGC_C
Cuts at: 351 1068 2055 2303 351
Size: 717 987 248 2673
Fragments arranged by size:
2673 987 717 248
KpnI G_GTAC'C
Cuts at: 877 1645 877
Size: 768 3857
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 707 3296 3480 707
Size: 2589 184 1852
Fragments arranged by size:
2589 1852 184
MscI TGG'CCA
Cuts at: 1120 1168 2386 1120
Size: 48 1218 3359
Fragments arranged by size:
3359 1218 48
MslI CAynn'nnrTG
Cuts at: 1096 2614 2741 1096
Size: 1518 127 2980
Fragments arranged by size:
2980 1518 127
MspA1I CmG'CkG
Cuts at: 366 434 816 1331 1352 1361 1515 1563 1736
Size: 68 382 515 21 9 154 48 173
Cuts at: 1736 2050 2410 3423 3668 366
Size: 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 515 382 360 314 245 173
154 68 48 21 9
NaeI GCC'GGC
Cuts at: 728 2806 728
Size: 2078 2547
NarI GG'CG_CC
Cuts at: 352 1069 2056 2304 352
Size: 717 987 248 2673
Fragments arranged by size:
2673 987 717 248
NgoMIV G'CCGG_C
Cuts at: 726 2804 726
Size: 2078 2547
NspI r_CATG'y
Cuts at: 147 562 1147 1678 2178 3085 147
Size: 415 585 531 500 907 1687
Fragments arranged by size:
1687 907 585 531 500 415
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 113 943 113
Size: 830 3795
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 1606 1606
Size: 4625
PspOMI G'GGCC_C
Cuts at: 1174 1174
Size: 4625
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 733 765 733
Size: 32 4593
PstI C_TGCA'G
Cuts at: 266 1562 1672 2357 266
Size: 1296 110 685 2534
Fragments arranged by size:
2534 1296 685 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 366 1515 1563 2050 2410 366
Size: 1149 48 487 360 2581
Fragments arranged by size:
2581 1149 487 360 48
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1639 1639
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SanDI GG'GwC_CC
Cuts at: 1606 1606
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 956 2648 2858 956
Size: 1692 210 2723
Fragments arranged by size:
2723 1692 210
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 1412 4142 1412
Size: 2730 1895
SexAI A'CCwGG_T
Cuts at: 112 112
Size: 4625
SfcI C'TryA_G
Cuts at: 33 262 799 1558 1668 2353 3346 3537 33
Size: 229 537 759 110 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 537 229 191 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 1493 1502 1493
Size: 9 4616
SfoI GGC'GCC
Cuts at: 353 1070 2057 2305 353
Size: 717 987 248 2673
Fragments arranged by size:
2673 987 717 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 736 1780 2695 3187 3449 736
Size: 1044 915 492 262 1912
Fragments arranged by size:
1912 1044 915 492 262
SnaBI TAC'GTA
Cuts at: 855 855
Size: 4625
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 147 562 1147 1678 2178 147
Size: 415 585 531 500 2594
Fragments arranged by size:
2594 585 531 500 415
StuI AGG'CCT
Cuts at: 1556 1556
Size: 4625
StyI C'CwwG_G
Cuts at: 255 1759 2113 255
Size: 1504 354 2767
Fragments arranged by size:
2767 1504 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 670 977 1389 1452 2208 2341 2800 3009 670
Size: 307 412 63 756 133 459 209 2286
Fragments arranged by size:
2286 756 459 412 307 209 133 63
TatI w'GTAC_w
Cuts at: 1205 1410 4140 1205
Size: 205 2730 1690
Fragments arranged by size:
2730 1690 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1142 1174 4142 4174 1142
Size: 32 2968 32 1593
Fragments arranged by size:
2968 1593 32 32
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2120 114
Size: 2006 2619
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspXI SacII SgrAI SrfI SspI
SwaI XhoI XmnI ZraI