Restriction map for
pF1KA1853
(Circular) (Six-base) MAPSORT of: pF1KA1853.seq Check: 6660 from: 1 to: 4946
pF1KA1853 4946 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1569 1569
Size: 4946
AccI GT'mk_AC
Cuts at: 1984 1984
Size: 4946
Acc65I G'GTAC_C
Cuts at: 1962 1962
Size: 4946
AclI AA'CG_TT
Cuts at: 4786 4786
Size: 4946
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 3203 3556 4218 2771
Size: 432 353 662 3499
Fragments arranged by size:
3499 662 432 353
AflIII A'CryG_T
Cuts at: 977 2298 4098 4379 977
Size: 1321 1800 281 1544
Fragments arranged by size:
1800 1544 1321 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 247 247
Size: 4946
AlwNI CAG_nnn'CTG
Cuts at: 306 2251 3689 306
Size: 1945 1438 1563
Fragments arranged by size:
1945 1563 1438
ApaLI G'TGCA_C
Cuts at: 3784 3784
Size: 4946
ApoI r'AATT_y
Cuts at: 1950 4299 4555 1950
Size: 2349 256 2341
Fragments arranged by size:
2349 2341 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4946
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
AvaI C'yCGr_G
Cuts at: 190 1019 1608 1966 190
Size: 829 589 358 3170
Fragments arranged by size:
3170 829 589 358
BamHI G'GATC_C
Cuts at: 1506 1971 1506
Size: 465 4481
BanI G'GyrC_C
Cuts at: 325 367 722 1962 2376 2624 2659 325
Size: 42 355 1240 414 248 35 2612
Fragments arranged by size:
2612 1240 414 355 248 42 35
BanII G_rGCy'C
Cuts at: 248 320 691 1042 1073 1258 1438 1843 1960
Size: 72 371 351 31 185 180 405 117
Cuts at: 1960 2990 248
Size: 1030 2204
Fragments arranged by size:
2204 1030 405 371 351 185 180 117
72 31
BbeI G_GCGC'C
Cuts at: 2380 2628 2380
Size: 248 4698
BbvCI CC'TCA_GC
Cuts at: 1696 1696
Size: 4946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 186 220 186
Size: 34 4912
BciVI GTATCCnnnnn_n'
Cuts at: 2844 3900 4946 2844
Size: 1056 1046 2844
Fragments arranged by size:
2844 1056 1046
BclI T'GATC_A
Cuts at: 2466 2466
Size: 4946
BglII A'GATC_T
Cuts at: 2461 4498 2461
Size: 2037 2909
BlpI GC'TnA_GC
Cuts at: 1008 1911 2058 1008
Size: 903 147 3896
Fragments arranged by size:
3896 903 147
Bme1580I G_kGCm'C
Cuts at: 328 1644 2571 2664 3788 328
Size: 1316 927 93 1124 1486
Fragments arranged by size:
1486 1316 1124 927 93
BmgBI CAC'GTC
Cuts at: 980 980
Size: 4946
BmrI ACTGGGnnnn_n'
Cuts at: 373 810 827 893 1037 2158 2239 2574 4718
Size: 437 17 66 144 1121 81 335 2144
Cuts at: 4718 373
Size: 601
Fragments arranged by size:
2144 1121 601 437 335 144 81 66
17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 981 1170 1545 3313 981
Size: 189 375 1768 2614
Fragments arranged by size:
2614 1768 375 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 829 1293 1639 1785 1911 408
Size: 421 464 346 146 126 3443
Fragments arranged by size:
3443 464 421 346 146 126
Bpu10I CC'TnA_GC
Cuts at: 272 1696 272
Size: 1424 3522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 1074 2122 3001 3715 4013 690
Size: 384 1048 879 714 298 1623
Fragments arranged by size:
1623 1048 879 714 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 1022 4650 1022
Size: 3628 1318
BsaAI yAC'GTr
Cuts at: 2299 2299
Size: 4946
BsaBI GATnn'nnATC
Cuts at: 6 2460 4497 6
Size: 2454 2037 455
Fragments arranged by size:
2454 2037 455
BsaHI Gr'CG_yC
Cuts at: 1566 2377 2625 3327 4823 1566
Size: 811 248 702 1496 1689
Fragments arranged by size:
1689 1496 811 702 248
BsaWI w'CCGG_w
Cuts at: 2135 2656 3334 3745 3892 4166 2135
Size: 521 678 411 147 274 2915
Fragments arranged by size:
2915 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1029 1059 1752 1782 1909 1939 1029
Size: 30 693 30 127 30 4036
Fragments arranged by size:
4036 693 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 564 1107 1275 1446 1671 1698 564
Size: 543 168 171 225 27 3812
Fragments arranged by size:
3812 543 225 171 168 27
BseYI C'CCAG_C
Cuts at: 267 294 1405 1441 1492 1672 2371 3129 3794
Size: 27 1111 36 51 180 699 758 665
Cuts at: 3794 4202 267
Size: 408 1011
Fragments arranged by size:
1111 1011 758 699 665 408 180 51
36 27
BsiEI CG_ry'CG
Cuts at: 104 732 2488 2534 3764 104
Size: 628 1756 46 1230 1286
Fragments arranged by size:
1756 1286 1230 628 46
BsiHKAI G_wGCw'C
Cuts at: 691 1073 1258 1960 2738 2928 3788 691
Size: 382 185 702 778 190 860 1849
Fragments arranged by size:
1849 860 778 702 382 190 185
BsmBI CGTCTCn'nnnn_
Cuts at: 1557 1779 1557
Size: 222 4724
Bsp1286I G_dGCh'C
Cuts at: 248 320 328 691 1042 1073 1258 1438 1644
Size: 72 8 363 351 31 185 180 206
Cuts at: 1644 1843 1960 2571 2664 2738 2928 2990 3788
Size: 199 117 611 93 74 190 62 798
Cuts at: 3788 248
Size: 1406
Fragments arranged by size:
1406 798 611 363 351 206 199 190
185 180 117 93 74 72 62 31
8
BspHI T'CATG_A
Cuts at: 393 3378 393
Size: 2985 1961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 1996 2210 2512 2893 533
Size: 1463 214 302 381 2586
Fragments arranged by size:
2586 1463 381 302 214
BsrBI CCG'CTC
Cuts at: 50 930 1400 1503 1736 3238 3292 4776 4941
Size: 880 470 103 233 1502 54 1484 165
Cuts at: 4941 50
Size: 55
Fragments arranged by size:
1502 1484 880 470 233 165 103 55
54
BsrDI GCAATG_nn'
Cuts at: 2858 2858
Size: 4946
BsrFI r'CCGG_y
Cuts at: 632 731 2944 3125 632
Size: 99 2213 181 2453
Fragments arranged by size:
2453 2213 181 99
BssSI C'ACGA_G
Cuts at: 1803 1932 3217 3925 1803
Size: 129 1285 708 2824
Fragments arranged by size:
2824 1285 708 129
BstAPI GCAn_nnn'nTGC
Cuts at: 2323 2323
Size: 4946
BstBI TT'CG_AA
Cuts at: 3307 3307
Size: 4946
BstXI CCAn_nnnn'nTGG
Cuts at: 853 853
Size: 4946
BstYI r'GATC_y
Cuts at: 1 1506 1971 2438 2461 2796 3042 3348 3360
Size: 1505 465 467 23 335 246 306 12
Cuts at: 3360 3446 4498 1
Size: 86 1052 449
Fragments arranged by size:
1505 1052 467 465 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 740 809 740
Size: 69 4877
BtgI C'CryG_G
Cuts at: 107 726 107
Size: 619 4327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 476 2896 3077 3240 476
Size: 2420 181 163 2182
Fragments arranged by size:
2420 2182 181 163
BtsI GCAGTG_nn'
Cuts at: 2189 2189
Size: 4946
DraI TTT'AAA
Cuts at: 1945 1945
Size: 4946
DrdI GACnn_nn'nnGTC
Cuts at: 2652 3996 2652
Size: 1344 3602
EaeI y'GGCC_r
Cuts at: 176 308 729 1739 2531 2705 3096 3123 4468
Size: 132 421 1010 792 174 391 27 1345
Cuts at: 4468 176
Size: 654
Fragments arranged by size:
1345 1010 792 654 421 391 174 132
27
EagI C'GGCC_G
Cuts at: 729 2531 729
Size: 1802 3144
EarI CTCTTCn'nnn_
Cuts at: 457 553 591 601 1380 2969 3179 457
Size: 96 38 10 779 1589 210 2224
Fragments arranged by size:
2224 1589 779 210 96 38 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3154 3896 4042 4756 3154
Size: 742 146 714 3344
Fragments arranged by size:
3344 742 714 146
EcoICRI GAG'CTC
Cuts at: 689 1071 1256 1958 689
Size: 382 185 702 3677
Fragments arranged by size:
3677 702 382 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 829 1293 1639 1785 1911 2771 3203 3556
Size: 421 464 346 146 126 860 432 353
Cuts at: 3556 4218 408
Size: 662 1136
Fragments arranged by size:
1136 860 662 464 432 421 353 346
146 126
EcoNI CCTnn'n_nnAGG
Cuts at: 743 796 743
Size: 53 4893
EcoO109I rG'GnC_Cy
Cuts at: 473 1388 1457 2085 473
Size: 915 69 628 3334
Fragments arranged by size:
3334 915 628 69
EcoRI G'AATT_C
Cuts at: 1950 1950
Size: 4946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4946
FspI TGC'GCA
Cuts at: 2727 2727
Size: 4946
HaeII r_GCGC'y
Cuts at: 1596 2380 2628 3858 4629 1596
Size: 784 248 1230 771 1913
Fragments arranged by size:
1913 1230 784 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 802 834 967 999 1752 1784 1909
Size: 32 502 32 133 32 753 32 125
Cuts at: 1909 1941 2788 2818 2820 2850 268
Size: 32 847 30 2 30 2364
Fragments arranged by size:
2364 847 753 502 133 125 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 838 1985 838
Size: 1147 3799
KasI G'GCGC_C
Cuts at: 2376 2624 2376
Size: 248 4698
KpnI G_GTAC'C
Cuts at: 1966 1966
Size: 4946
MluI A'CGCG_T
Cuts at: 4379 4379
Size: 4946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1540 1750 3705 3889 1124
Size: 416 210 1955 184 2181
Fragments arranged by size:
2181 1955 416 210 184
MscI TGG'CCA
Cuts at: 178 310 2707 178
Size: 132 2397 2417
Fragments arranged by size:
2417 2397 132
MslI CAynn'nnrTG
Cuts at: 2935 3062 2935
Size: 127 4819
MspA1I CmG'CkG
Cuts at: 231 668 728 779 1539 2057 2371 2731 3515
Size: 437 60 51 760 518 314 360 784
Cuts at: 3515 3760 231
Size: 245 1417
Fragments arranged by size:
1417 784 760 518 437 360 314 245
60 51
NaeI GCC'GGC
Cuts at: 634 3127 634
Size: 2493 2453
NarI GG'CG_CC
Cuts at: 2377 2625 2377
Size: 248 4698
NcoI C'CATG_G
Cuts at: 107 107
Size: 4946
NgoMIV G'CCGG_C
Cuts at: 632 3125 632
Size: 2493 2453
NruI TCG'CGA
Cuts at: 942 942
Size: 4946
NspI r_CATG'y
Cuts at: 1999 2499 4102 1999
Size: 500 1603 2843
Fragments arranged by size:
2843 1603 500
PasI CC'CwG_GG
Cuts at: 1337 1337
Size: 4946
PciI A'CATG_T
Cuts at: 4098 4098
Size: 4946
PflMI CCAn_nnn'nTGG
Cuts at: 335 335
Size: 4946
PfoI T'CCnGG_A
Cuts at: 1756 1756
Size: 4946
PmeI GTTT'AAAC
Cuts at: 1945 1945
Size: 4946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4914
PpuMI rG'GwC_Cy
Cuts at: 473 1388 1457 473
Size: 915 69 3962
Fragments arranged by size:
3962 915 69
PshAI GACnn'nnGTC
Cuts at: 543 1569 543
Size: 1026 3920
PstI C_TGCA'G
Cuts at: 1993 2678 1993
Size: 685 4261
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4946
PvuII CAG'CTG
Cuts at: 2371 2731 2371
Size: 360 4586
RsrII CG'GwC_CG
Cuts at: 3141 3141
Size: 4946
SacI G_AGCT'C
Cuts at: 691 1073 1258 1960 691
Size: 382 185 702 3677
Fragments arranged by size:
3677 702 382 185
SacII CC_GC'GG
Cuts at: 729 729
Size: 4946
SalI G'TCGA_C
Cuts at: 1983 1983
Size: 4946
SapI GCTCTTCn'nnn_
Cuts at: 2969 3179 2969
Size: 210 4736
SbfI CC_TGCA'GG
Cuts at: 1993 1993
Size: 4946
ScaI AGT'ACT
Cuts at: 4463 4463
Size: 4946
SfcI C'TryA_G
Cuts at: 33 483 1485 1905 1989 2674 3642 3833 33
Size: 450 1002 420 84 685 968 191 1146
Fragments arranged by size:
1146 1002 968 685 450 420 191 84
SfoI GGC'GCC
Cuts at: 2378 2626 2378
Size: 248 4698
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
SmaI CCC'GGG
Cuts at: 1968 1968
Size: 4946
SmlI C'TyrA_G
Cuts at: 705 1053 2101 3016 3730 3992 705
Size: 348 1048 915 714 262 1659
Fragments arranged by size:
1659 1048 915 714 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4946
SphI G_CATG'C
Cuts at: 1999 2499 1999
Size: 500 4446
StyI C'CwwG_G
Cuts at: 107 235 320 2080 2434 107
Size: 128 85 1760 354 2619
Fragments arranged by size:
2619 1760 354 128 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 1275 1372 1559 2529 2662 3121 3330 355
Size: 920 97 187 970 133 459 209 1971
Fragments arranged by size:
1971 970 920 459 209 187 133 97
TatI w'GTAC_w
Cuts at: 4461 4461
Size: 4946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 171 203 1029 1061 1623 1655 4463 4495 171
Size: 32 826 32 562 32 2808 32 622
Fragments arranged by size:
2808 826 622 562 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2743 2743
Size: 4946
XbaI T'CTAG_A
Cuts at: 1977 1977
Size: 4946
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 318 2441 318
Size: 2123 2823
XmaI C'CCGG_G
Cuts at: 1966 1966
Size: 4946
XmnI GAAnn'nnTTC
Cuts at: 1105 1105
Size: 4946
ZraI GAC'GTC
Cuts at: 1567 1567
Size: 4946
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmtI BsgI
BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PmlI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI