Restriction map for
pF1KA1861
(Circular) (Six-base) MAPSORT of: pF1KA1861.seq Check: 4610 from: 1 to: 5984
pF1KA1861 5984 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2097 2640 3022 2097
Size: 543 382 5059
Fragments arranged by size:
5059 543 382
Acc65I G'GTAC_C
Cuts at: 3000 3000
Size: 5984
AclI AA'CG_TT
Cuts at: 5824 5824
Size: 5984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1136 1181 1205 1409 2308 2356 3809 4241 4988
Size: 45 24 204 899 48 1453 432 747
Cuts at: 4988 5256 1136
Size: 268 1864
Fragments arranged by size:
1864 1453 899 747 432 268 204 48
45 24
AflII C'TTAA_G
Cuts at: 1969 1969
Size: 5984
AflIII A'CryG_T
Cuts at: 1029 3336 4440 5417 1029
Size: 2307 1104 977 1596
Fragments arranged by size:
2307 1596 1104 977
AgeI A'CCGG_T
Cuts at: 4372 4372
Size: 5984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1086 1369 2623 1086
Size: 283 1254 4447
Fragments arranged by size:
4447 1254 283
AlwNI CAG_nnn'CTG
Cuts at: 123 585 1159 2469 3289 4856 123
Size: 462 574 1310 820 1567 1251
Fragments arranged by size:
1567 1310 1251 820 574 462
ApaLI G'TGCA_C
Cuts at: 4754 4754
Size: 5984
ApoI r'AATT_y
Cuts at: 193 753 825 994 1369 1515 1540 1546 1661
Size: 560 72 169 375 146 25 6 115
Cuts at: 1661 2551 2788 2988 5337 5593 193
Size: 890 237 200 2349 256 584
Fragments arranged by size:
2349 890 584 560 375 256 237 200
169 146 115 72 25 6
AseI AT'TA_AT
Cuts at: 20 248 20
Size: 228 5756
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
AvaI C'yCGr_G
Cuts at: 1841 3004 1841
Size: 1163 4821
BamHI G'GATC_C
Cuts at: 2908 3009 2908
Size: 101 5883
BanI G'GyrC_C
Cuts at: 157 2202 3000 3414 3662 3697 157
Size: 2045 798 414 248 35 2444
Fragments arranged by size:
2444 2045 798 414 248 35
BanII G_rGCy'C
Cuts at: 2998 4028 2998
Size: 1030 4954
BbeI G_GCGC'C
Cuts at: 3418 3666 3418
Size: 248 5736
BbsI GAAGACnn'nnnn_
Cuts at: 463 513 463
Size: 50 5934
BciVI GTATCCnnnnn_n'
Cuts at: 1137 2241 3882 4643 5984 1137
Size: 1104 1641 761 1341 1137
Fragments arranged by size:
1641 1341 1137 1104 761
BclI T'GATC_A
Cuts at: 3504 3504
Size: 5984
BglII A'GATC_T
Cuts at: 3499 5536 3499
Size: 2037 3947
BlpI GC'TnA_GC
Cuts at: 1096 3096 1096
Size: 2000 3984
Bme1580I G_kGCm'C
Cuts at: 2712 3609 3702 4758 2712
Size: 897 93 1056 3938
Fragments arranged by size:
3938 1056 897 93
BmrI ACTGGGnnnn_n'
Cuts at: 3196 3277 3612 5756 3196
Size: 81 335 2144 3424
Fragments arranged by size:
3424 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4351 4351
Size: 5984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 679 328
Size: 351 5633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 212 524 3160 4039 4531 4829 119
Size: 93 312 2636 879 492 298 1274
Fragments arranged by size:
2636 1274 879 492 312 298 93
BsaI GGTCTCn'nnnn_
Cuts at: 5688 5688
Size: 5984
BsaAI yAC'GTr
Cuts at: 3337 3337
Size: 5984
BsaBI GATnn'nnATC
Cuts at: 6 3498 5535 6
Size: 3492 2037 455
Fragments arranged by size:
3492 2037 455
BsaHI Gr'CG_yC
Cuts at: 3415 3663 4365 5861 3415
Size: 248 702 1496 3538
Fragments arranged by size:
3538 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3173 3694 4372 4646 4793 5204 3173
Size: 521 678 274 147 411 3953
Fragments arranged by size:
3953 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 676
Size: 5984
BseYI C'CCAG_C
Cuts at: 3409 4167 4744 5240 3409
Size: 758 577 496 4153
Fragments arranged by size:
4153 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 995
Size: 5984
BsiEI CG_ry'CG
Cuts at: 104 3526 3572 4780 104
Size: 3422 46 1208 1308
Fragments arranged by size:
3422 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2858 2998 3776 3966 4758 2858
Size: 140 778 190 792 4084
Fragments arranged by size:
4084 792 778 190 140
BsmI GAATG_Cn'
Cuts at: 1899 1899
Size: 5984
Bsp1286I G_dGCh'C
Cuts at: 2712 2858 2998 3609 3702 3776 3966 4028 4758
Size: 146 140 611 93 74 190 62 730
Cuts at: 4758 2712
Size: 3938
Fragments arranged by size:
3938 730 611 190 146 140 93 74
62
BspEI T'CCGG_A
Cuts at: 5204 5204
Size: 5984
BspHI T'CATG_A
Cuts at: 1536 5160 1536
Size: 3624 2360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1337 3034 3248 3550 3931 1337
Size: 1697 214 302 381 3390
Fragments arranged by size:
3390 1697 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4276 4330 5814 5979 50
Size: 4226 54 1484 165 55
Fragments arranged by size:
4226 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 457 622 899 3896 457
Size: 165 277 2997 2545
Fragments arranged by size:
2997 2545 277 165
BsrFI r'CCGG_y
Cuts at: 3982 4163 4372 3982
Size: 181 209 5594
Fragments arranged by size:
5594 209 181
BsrGI T'GTAC_A
Cuts at: 493 1024 493
Size: 531 5453
BssSI C'ACGA_G
Cuts at: 4255 4613 4255
Size: 358 5626
BstAPI GCAn_nnn'nTGC
Cuts at: 3361 3361
Size: 5984
BstBI TT'CG_AA
Cuts at: 4345 4345
Size: 5984
BstXI CCAn_nnnn'nTGG
Cuts at: 2429 2429
Size: 5984
BstYI r'GATC_y
Cuts at: 1 1017 2908 3009 3476 3499 3834 4080 5092
Size: 1016 1891 101 467 23 335 246 1012
Cuts at: 5092 5178 5190 5536 1
Size: 86 12 346 449
Fragments arranged by size:
1891 1016 1012 467 449 346 335 246
101 86 23 12
BstZ17I GTA'TAC
Cuts at: 2641 2641
Size: 5984
Bsu36I CC'TnA_GG
Cuts at: 1778 1778
Size: 5984
BtgI C'CryG_G
Cuts at: 2282 2282
Size: 5984
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3934 4115 4278 3934
Size: 181 163 5640
Fragments arranged by size:
5640 181 163
BtsI GCAGTG_nn'
Cuts at: 3227 3227
Size: 5984
DraI TTT'AAA
Cuts at: 726 1545 2983 726
Size: 819 1438 3727
Fragments arranged by size:
3727 1438 819
DraIII CAC_nnn'GTG
Cuts at: 2229 2229
Size: 5984
DrdI GACnn_nn'nnGTC
Cuts at: 3690 4548 3690
Size: 858 5126
EaeI y'GGCC_r
Cuts at: 3569 3743 4134 4161 5506 3569
Size: 174 391 27 1345 4047
Fragments arranged by size:
4047 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3569 3569
Size: 5984
EarI CTCTTCn'nnn_
Cuts at: 4007 4217 4007
Size: 210 5774
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4192 4502 4648 5794 4192
Size: 310 146 1146 4382
Fragments arranged by size:
4382 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2996 2996
Size: 5984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 679 1136 1181 1205 1409 2308 2356 3809
Size: 351 457 45 24 204 899 48 1453
Cuts at: 3809 4241 4988 5256 328
Size: 432 747 268 1056
Fragments arranged by size:
1453 1056 899 747 457 432 351 268
204 48 45 24
EcoO109I rG'GnC_Cy
Cuts at: 177 3123 177
Size: 2946 3038
EcoRI G'AATT_C
Cuts at: 193 753 2988 193
Size: 560 2235 3189
Fragments arranged by size:
3189 2235 560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 970 2149 4003 970
Size: 1179 1854 2951
Fragments arranged by size:
2951 1854 1179
FspI TGC'GCA
Cuts at: 3765 3765
Size: 5984
HaeII r_GCGC'y
Cuts at: 3418 3666 4688 5667 3418
Size: 248 1022 979 3735
Fragments arranged by size:
3735 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1731 1763 2900 2932 3826 3856 3858 3888 1731
Size: 32 1137 32 894 30 2 30 3827
Fragments arranged by size:
3827 1137 894 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3023 3023
Size: 5984
HindIII A'AGCT_T
Cuts at: 313 860 2810 313
Size: 547 1950 3487
Fragments arranged by size:
3487 1950 547
KasI G'GCGC_C
Cuts at: 3414 3662 3414
Size: 248 5736
KpnI G_GTAC'C
Cuts at: 3004 3004
Size: 5984
MfeI C'AATT_G
Cuts at: 1976 2719 1976
Size: 743 5241
MluI A'CGCG_T
Cuts at: 5417 5417
Size: 5984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1341 1920 2032 4655 4839 1341
Size: 579 112 2623 184 2486
Fragments arranged by size:
2623 2486 579 184 112
MscI TGG'CCA
Cuts at: 3745 3745
Size: 5984
MslI CAynn'nnrTG
Cuts at: 1136 1245 1679 2336 3973 4100 1136
Size: 109 434 657 1637 127 3020
Fragments arranged by size:
3020 1637 657 434 127 109
MspA1I CmG'CkG
Cuts at: 795 2155 2880 3095 3409 3769 4782 5027 795
Size: 1360 725 215 314 360 1013 245 1752
Fragments arranged by size:
1752 1360 1013 725 360 314 245 215
NaeI GCC'GGC
Cuts at: 4165 4165
Size: 5984
NarI GG'CG_CC
Cuts at: 3415 3663 3415
Size: 248 5736
NdeI CA'TA_TG
Cuts at: 908 908
Size: 5984
NgoMIV G'CCGG_C
Cuts at: 4163 4163
Size: 5984
NspI r_CATG'y
Cuts at: 1033 3037 3537 4444 1033
Size: 2004 500 907 2573
Fragments arranged by size:
2573 2004 907 500
PciI A'CATG_T
Cuts at: 1029 4440 1029
Size: 3411 2573
PfoI T'CCnGG_A
Cuts at: 675 675
Size: 5984
PmeI GTTT'AAAC
Cuts at: 2983 2983
Size: 5984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 5123
Size: 32 5952
PpuMI rG'GwC_Cy
Cuts at: 177 177
Size: 5984
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2624 2656 2624
Size: 32 5952
PstI C_TGCA'G
Cuts at: 384 2355 3031 3716 384
Size: 1971 676 685 2652
Fragments arranged by size:
2652 1971 685 676
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5984
PvuII CAG'CTG
Cuts at: 795 2155 2880 3409 3769 795
Size: 1360 725 529 360 3010
Fragments arranged by size:
3010 1360 725 529 360
RsrII CG'GwC_CG
Cuts at: 4179 4179
Size: 5984
SacI G_AGCT'C
Cuts at: 2998 2998
Size: 5984
SalI G'TCGA_C
Cuts at: 3021 3021
Size: 5984
SanDI GG'GwC_CC
Cuts at: 177 177
Size: 5984
SapI GCTCTTCn'nnn_
Cuts at: 4007 4217 4007
Size: 210 5774
SbfI CC_TGCA'GG
Cuts at: 3031 3031
Size: 5984
ScaI AGT'ACT
Cuts at: 1636 5501 1636
Size: 3865 2119
SfcI C'TryA_G
Cuts at: 33 380 1638 2351 2951 3027 3712 4705 4896
Size: 347 1258 713 600 76 685 993 191
Cuts at: 4896 33
Size: 1121
Fragments arranged by size:
1258 1121 993 713 685 600 347 191
76
SfoI GGC'GCC
Cuts at: 3416 3664 3416
Size: 248 5736
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
SmaI CCC'GGG
Cuts at: 3006 3006
Size: 5984
SmlI C'TyrA_G
Cuts at: 134 227 539 1969 3139 4054 4546 4808 134
Size: 93 312 1430 1170 915 492 262 1310
Fragments arranged by size:
1430 1310 1170 915 492 312 262 93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5984
SphI G_CATG'C
Cuts at: 3037 3537 3037
Size: 500 5484
SspI AAT'ATT
Cuts at: 519 2866 519
Size: 2347 3637
StuI AGG'CCT
Cuts at: 554 554
Size: 5984
StyI C'CwwG_G
Cuts at: 848 2291 3118 3472 848
Size: 1443 827 354 3360
Fragments arranged by size:
3360 1443 827 354
SwaI ATTT'AAAT
Cuts at: 1545 1545
Size: 5984
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 934 3567 3700 4159 4368 934
Size: 2633 133 459 209 2550
Fragments arranged by size:
2633 2550 459 209 133
TatI w'GTAC_w
Cuts at: 493 1024 1634 5499 493
Size: 531 610 3865 978
Fragments arranged by size:
3865 978 610 531
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5501 5533 5501
Size: 32 5952
Tth111I GACn'n_nGTC
Cuts at: 119 3781 119
Size: 3662 2322
XbaI T'CTAG_A
Cuts at: 2142 3015 2142
Size: 873 5111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2289 3479 2289
Size: 1190 4794
XmaI C'CCGG_G
Cuts at: 3004 3004
Size: 5984
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BssHII BstEII ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI
XhoI XmnI ZraI