Restriction map for
pF1KA1866
(Circular) (Six-base) MAPSORT of: pF1KA1866.seq Check: 5224 from: 1 to: 8432
pF1KA1866 8432 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3087 3115 3087
Size: 28 8404
AatII G_ACGT'C
Cuts at: 4453 4453
Size: 8432
AccI GT'mk_AC
Cuts at: 153 683 1372 1473 2314 5470 153
Size: 530 689 101 841 3156 3115
Fragments arranged by size:
3156 3115 841 689 530 101
Acc65I G'GTAC_C
Cuts at: 3875 5448 3875
Size: 1573 6859
AclI AA'CG_TT
Cuts at: 8272 8272
Size: 8432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 4379 4559 4708 6257 6689 7436 7704 1351
Size: 3028 180 149 1549 432 747 268 2079
Fragments arranged by size:
3028 2079 1549 747 432 268 180 149
AfeI AGC'GCT
Cuts at: 1532 3558 1532
Size: 2026 6406
AflII C'TTAA_G
Cuts at: 2487 2487
Size: 8432
AflIII A'CryG_T
Cuts at: 1631 2687 5784 6888 7865 1631
Size: 1056 3097 1104 977 2198
Fragments arranged by size:
3097 2198 1104 1056 977
AgeI A'CCGG_T
Cuts at: 6820 6820
Size: 8432
AhdI GACnn_n'nnGTC
Cuts at: 468 3138 3348 468
Size: 2670 210 5552
Fragments arranged by size:
5552 2670 210
AleI CACnn'nnGTG
Cuts at: 4735 4735
Size: 8432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 4843 4875 2536
Size: 32 2275 32 6093
Fragments arranged by size:
6093 2275 32 32
AlwNI CAG_nnn'CTG
Cuts at: 937 1021 1039 1114 2184 5737 7304 937
Size: 84 18 75 1070 3553 1567 2065
Fragments arranged by size:
3553 2065 1567 1070 84 75 18
ApaI G_GGCC'C
Cuts at: 1521 2193 2766 3042 1521
Size: 672 573 276 6911
Fragments arranged by size:
6911 672 573 276
ApaLI G'TGCA_C
Cuts at: 1822 7202 1822
Size: 5380 3052
ApoI r'AATT_y
Cuts at: 946 4441 4546 5436 7785 8041 946
Size: 3495 105 890 2349 256 1337
Fragments arranged by size:
3495 2349 1337 890 256 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 8432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8432
AvaI C'yCGr_G
Cuts at: 1406 2327 2471 2811 3206 4158 5452 1406
Size: 921 144 340 395 952 1294 4386
Fragments arranged by size:
4386 1294 952 921 395 340 144
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4382 4415 5385 5418 4382
Size: 33 970 33 7396
Fragments arranged by size:
7396 970 33 33
BamHI G'GATC_C
Cuts at: 795 4340 5045 5457 795
Size: 3545 705 412 3770
Fragments arranged by size:
3770 3545 705 412
BanI G'GyrC_C
Cuts at: 486 1317 1596 1645 1744 3583 3875 4519 5448
Size: 831 279 49 99 1839 292 644 929
Cuts at: 5448 5862 6110 6145 486
Size: 414 248 35 2773
Fragments arranged by size:
2773 1839 929 831 644 414 292 279
248 99 49 35
BanII G_rGCy'C
Cuts at: 1215 1521 1729 1975 2193 2334 2766 2941 3042
Size: 306 208 246 218 141 432 175 101
Cuts at: 3042 3574 4333 5446 6476 1215
Size: 532 759 1113 1030 3171
Fragments arranged by size:
3171 1113 1030 759 532 432 306 246
218 208 175 141 101
BbeI G_GCGC'C
Cuts at: 1321 1600 1748 4523 5866 6114 1321
Size: 279 148 2775 1343 248 3639
Fragments arranged by size:
3639 2775 1343 279 248 148
BbsI GAAGACnn'nnnn_
Cuts at: 993 2153 3344 3347 4844 5237 993
Size: 1160 1191 3 1497 393 4188
Fragments arranged by size:
4188 1497 1191 1160 393 3
BbvCI CC'TCA_GC
Cuts at: 1899 1899
Size: 8432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3000 3034 4122 4156 3000
Size: 34 1088 34 7276
Fragments arranged by size:
7276 1088 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2395 6330 7091 8432 2395
Size: 3935 761 1341 2395
Fragments arranged by size:
3935 2395 1341 761
BclI T'GATC_A
Cuts at: 5952 5952
Size: 8432
BfrBI ATG'CAT
Cuts at: 3672 3672
Size: 8432
BglI GCCn_nnn'nGGC
Cuts at: 1138 1348 1849 2605 3067 3185 3369 1138
Size: 210 501 756 462 118 184 6201
Fragments arranged by size:
6201 756 501 462 210 184 118
BglII A'GATC_T
Cuts at: 3942 5947 7984 3942
Size: 2005 2037 4390
Fragments arranged by size:
4390 2037 2005
BlpI GC'TnA_GC
Cuts at: 2374 5544 2374
Size: 3170 5262
Bme1580I G_kGCm'C
Cuts at: 425 1521 1648 1826 2193 2324 2766 3042 3586
Size: 1096 127 178 367 131 442 276 544
Cuts at: 3586 6057 6150 7206 425
Size: 2471 93 1056 1651
Fragments arranged by size:
2471 1651 1096 1056 544 442 367 276
178 131 127 93
BmgBI CAC'GTC
Cuts at: 2798 2894 3531 2798
Size: 96 637 7699
Fragments arranged by size:
7699 637 96
BmrI ACTGGGnnnn_n'
Cuts at: 1394 2726 3189 4785 5644 5725 6060 8204 1394
Size: 1332 463 1596 859 81 335 2144 1622
Fragments arranged by size:
2144 1622 1596 1332 859 463 335 81
BmtI G_CTAG'C
Cuts at: 619 619
Size: 8432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 249 1212 2968 3491 6799 249
Size: 963 1756 523 3308 1882
Fragments arranged by size:
3308 1882 1756 963 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 521 1199 2283 2439 2925 2950 3488 362
Size: 159 678 1084 156 486 25 538 5306
Fragments arranged by size:
5306 1084 678 538 486 159 156 25
Bpu10I CC'TnA_GC
Cuts at: 1187 1899 1969 2369 2379 2609 1187
Size: 712 70 400 10 230 7010
Fragments arranged by size:
7010 712 400 230 70 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1896 3800 4850 5608 6487 6979 7277 1896
Size: 1904 1050 758 879 492 298 3051
Fragments arranged by size:
3051 1904 1050 879 758 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 645 2097 2258 3291 4983 5412 8136 645
Size: 1452 161 1033 1692 429 2724 941
Fragments arranged by size:
2724 1692 1452 1033 941 429 161
BsaAI yAC'GTr
Cuts at: 125 2442 4587 5114 5785 125
Size: 2317 2145 527 671 2772
Fragments arranged by size:
2772 2317 2145 671 527
BsaBI GATnn'nnATC
Cuts at: 6 2570 5946 7983 6
Size: 2564 3376 2037 455
Fragments arranged by size:
3376 2564 2037 455
BsaHI Gr'CG_yC
Cuts at: 1318 1597 1745 2009 3404 4135 4200 4450 4520
Size: 279 148 264 1395 731 65 250 70
Cuts at: 4520 5863 6111 6813 8309 1318
Size: 1343 248 702 1496 1441
Fragments arranged by size:
1496 1441 1395 1343 731 702 279 264
250 248 148 70 65
BsaWI w'CCGG_w
Cuts at: 449 5621 6142 6820 7094 7241 7652 449
Size: 5172 521 678 274 147 411 1229
Fragments arranged by size:
5172 1229 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1020 1050 3679 3709 4787 4817 1020
Size: 30 2629 30 1078 30 4635
Fragments arranged by size:
4635 2629 1078 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1369 1405 1408 2046 2413 2442 2580 2774 2904
Size: 36 3 638 367 29 138 194 130
Cuts at: 2904 2907 3316 3319 3322 3935 1369
Size: 3 409 3 3 613 5866
Fragments arranged by size:
5866 638 613 409 367 194 138 130
36 29 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1331 1398 1754 1777 1958 2013 2477 2801 2849
Size: 67 356 23 181 55 464 324 48
Cuts at: 2849 3143 3175 3214 3275 4709 5071 5216 5857
Size: 294 32 39 61 1434 362 145 641
Cuts at: 5857 6615 7192 7688 1331
Size: 758 577 496 2075
Fragments arranged by size:
2075 1434 758 641 577 496 464 362
356 324 294 181 145 67 61 55
48 39 32 23
BsiEI CG_ry'CG
Cuts at: 104 2760 3544 5974 6020 7228 104
Size: 2656 784 2430 46 1208 1308
Fragments arranged by size:
2656 2430 1308 1208 784 46
BsiHKAI G_wGCw'C
Cuts at: 1215 1826 1975 2629 2941 4333 4624 5446 6224
Size: 611 149 654 312 1392 291 822 778
Cuts at: 6224 6414 7206 1215
Size: 190 792 2441
Fragments arranged by size:
2441 1392 822 792 778 654 611 312
291 190 149
BsmI GAATG_Cn'
Cuts at: 225 3867 225
Size: 3642 4790
BsmBI CGTCTCn'nnnn_
Cuts at: 2067 2387 2822 4441 2067
Size: 320 435 1619 6058
Fragments arranged by size:
6058 1619 435 320
Bsp1286I G_dGCh'C
Cuts at: 425 1215 1521 1648 1729 1826 1975 2193 2324
Size: 790 306 127 81 97 149 218 131
Cuts at: 2324 2334 2629 2766 2941 3042 3574 3586 4333
Size: 10 295 137 175 101 532 12 747
Cuts at: 4333 4624 5446 6057 6150 6224 6414 6476 7206
Size: 291 822 611 93 74 190 62 730
Cuts at: 7206 425
Size: 1651
Fragments arranged by size:
1651 822 790 747 730 611 532 306
295 291 218 190 175 149 137 131
127 101 97 93 81 74 62 12
10
BspEI T'CCGG_A
Cuts at: 7652 7652
Size: 8432
BspHI T'CATG_A
Cuts at: 7608 7608
Size: 8432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2079 2252 3087 3115 4709 5482 5696 5998 6379
Size: 173 835 28 1594 773 214 302 381
Cuts at: 6379 2079
Size: 4132
Fragments arranged by size:
4132 1594 835 773 381 302 214 173
28
BsrBI CCG'CTC
Cuts at: 50 2104 2906 6724 6778 8262 8427 50
Size: 2054 802 3818 54 1484 165 55
Fragments arranged by size:
3818 2054 1484 802 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 5235 6344 173
Size: 5062 1109 2261
Fragments arranged by size:
5062 2261 1109
BsrFI r'CCGG_y
Cuts at: 1848 6430 6611 6820 1848
Size: 4582 181 209 3460
Fragments arranged by size:
4582 3460 209 181
BssHII G'CGCG_C
Cuts at: 1716 3055 3057 3612 1716
Size: 1339 2 555 6536
Fragments arranged by size:
6536 1339 555 2
BssSI C'ACGA_G
Cuts at: 4914 6703 7061 4914
Size: 1789 358 6285
Fragments arranged by size:
6285 1789 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5809 5809
Size: 8432
BstBI TT'CG_AA
Cuts at: 6793 6793
Size: 8432
BstEII G'GTnAC_C
Cuts at: 2840 3130 2840
Size: 290 8142
BstYI r'GATC_y
Cuts at: 1 795 1362 3942 4340 5045 5457 5924 5947
Size: 794 567 2580 398 705 412 467 23
Cuts at: 5947 6282 6528 7540 7626 7638 7984 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2580 1012 794 705 567 467 449 412
398 346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1459 2576 1459
Size: 1117 7315
BtgI C'CryG_G
Cuts at: 107 1929 2127 2171 2388 2957 3598 3959 4733
Size: 1822 198 44 217 569 641 361 774
Cuts at: 4733 4742 5088 107
Size: 9 346 3451
Fragments arranged by size:
3451 1822 774 641 569 361 346 217
198 44 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3429 6382 6563 6726 3429
Size: 2953 181 163 5135
Fragments arranged by size:
5135 2953 181 163
BtsI GCAGTG_nn'
Cuts at: 1517 4169 4391 4484 5675 1517
Size: 2652 222 93 1191 4274
Fragments arranged by size:
4274 2652 1191 222 93
DraI TTT'AAA
Cuts at: 3327 4929 5431 3327
Size: 1602 502 6328
Fragments arranged by size:
6328 1602 502
DraIII CAC_nnn'GTG
Cuts at: 970 1488 970
Size: 518 7914
DrdI GACnn_nn'nnGTC
Cuts at: 751 6138 6996 751
Size: 5387 858 2187
Fragments arranged by size:
5387 2187 858
EaeI y'GGCC_r
Cuts at: 555 872 1057 1479 1574 1762 2757 3595 3624
Size: 317 185 422 95 188 995 838 29
Cuts at: 3624 4016 4739 6017 6191 6582 6609 7954 555
Size: 392 723 1278 174 391 27 1345 1033
Fragments arranged by size:
1345 1278 1033 995 838 723 422 392
391 317 188 185 174 95 29 27
EagI C'GGCC_G
Cuts at: 2757 6017 2757
Size: 3260 5172
EarI CTCTTCn'nnn_
Cuts at: 713 2070 3381 3422 3908 4403 4687 5217 6455
Size: 1357 1311 41 486 495 284 530 1238
Cuts at: 6455 6665 713
Size: 210 2480
Fragments arranged by size:
2480 1357 1311 1238 530 495 486 284
210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1156 2168 2179 6640 6950 7096 8242 1156
Size: 1012 11 4461 310 146 1146 1346
Fragments arranged by size:
4461 1346 1146 1012 310 146 11
EcoICRI GAG'CTC
Cuts at: 1213 1973 2939 4331 5444 1213
Size: 760 966 1392 1113 4201
Fragments arranged by size:
4201 1392 1113 966 760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 521 1199 1351 2283 2439 2925 2950 3488
Size: 159 678 152 932 156 486 25 538
Cuts at: 3488 4379 4559 4708 6257 6689 7436 7704 362
Size: 891 180 149 1549 432 747 268 1090
Fragments arranged by size:
1549 1090 932 891 747 678 538 486
432 268 180 159 156 152 149 25
EcoNI CCTnn'n_nnAGG
Cuts at: 244 2454 3421 3461 244
Size: 2210 967 40 5215
Fragments arranged by size:
5215 2210 967 40
EcoO109I rG'GnC_Cy
Cuts at: 129 1272 1517 1859 2281 2763 2815 3039 3140
Size: 1143 245 342 422 482 52 224 101
Cuts at: 3140 3210 3521 3953 4289 5021 5275 5571 129
Size: 70 311 432 336 732 254 296 2990
Fragments arranged by size:
2990 1143 732 482 432 422 342 336
311 296 254 245 224 101 70 52
EcoRI G'AATT_C
Cuts at: 5436 5436
Size: 8432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1581 1764 3361 6451 1581
Size: 183 1597 3090 3562
Fragments arranged by size:
3562 3090 1597 183
FspI TGC'GCA
Cuts at: 6213 6213
Size: 8432
HaeII r_GCGC'y
Cuts at: 1321 1534 1600 1748 3229 3560 4523 5866 6114
Size: 213 66 148 1481 331 963 1343 248
Cuts at: 6114 7136 8115 1321
Size: 1022 979 1638
Fragments arranged by size:
1638 1481 1343 1022 979 963 331 248
213 148 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 1354 1386 1801 1833 3124 3156 3334
Size: 32 868 32 415 32 1291 32 178
Cuts at: 3334 3366 4357 4389 4787 4819 6274 6304 6306
Size: 32 991 32 398 32 1455 30 2
Cuts at: 6306 6336 454
Size: 30 2550
Fragments arranged by size:
2550 1455 1291 991 868 415 398 178
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1373 2315 5471 1373
Size: 942 3156 4334
Fragments arranged by size:
4334 3156 942
HindIII A'AGCT_T
Cuts at: 2371 4868 2371
Size: 2497 5935
KasI G'GCGC_C
Cuts at: 1317 1596 1744 4519 5862 6110 1317
Size: 279 148 2775 1343 248 3639
Fragments arranged by size:
3639 2775 1343 279 248 148
KpnI G_GTAC'C
Cuts at: 3879 5452 3879
Size: 1573 6859
MluI A'CGCG_T
Cuts at: 1631 2687 7865 1631
Size: 1056 5178 2198
Fragments arranged by size:
5178 2198 1056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3218 4228 7103 7287 3218
Size: 1010 2875 184 4363
Fragments arranged by size:
4363 2875 1010 184
MscI TGG'CCA
Cuts at: 874 1059 1576 3626 4018 6193 874
Size: 185 517 2050 392 2175 3113
Fragments arranged by size:
3113 2175 2050 517 392 185
MslI CAynn'nnrTG
Cuts at: 214 2732 3643 4111 4307 4732 4735 5261 6421
Size: 2518 911 468 196 425 3 526 1160
Cuts at: 6421 6548 214
Size: 127 2098
Fragments arranged by size:
2518 2098 1160 911 526 468 425 196
127 3
MspA1I CmG'CkG
Cuts at: 3073 3600 4062 5216 5543 5857 6217 7230 7475
Size: 527 462 1154 327 314 360 1013 245
Cuts at: 7475 3073
Size: 4030
Fragments arranged by size:
4030 1154 1013 527 462 360 327 314
245
NaeI GCC'GGC
Cuts at: 1850 6613 1850
Size: 4763 3669
NarI GG'CG_CC
Cuts at: 1318 1597 1745 4520 5863 6111 1318
Size: 279 148 2775 1343 248 3639
Fragments arranged by size:
3639 2775 1343 279 248 148
NcoI C'CATG_G
Cuts at: 107 1929 2388 5088 107
Size: 1822 459 2700 3451
Fragments arranged by size:
3451 2700 1822 459
NgoMIV G'CCGG_C
Cuts at: 1848 6611 1848
Size: 4763 3669
NheI G'CTAG_C
Cuts at: 615 615
Size: 8432
NsiI A_TGCA'T
Cuts at: 3674 3674
Size: 8432
NspI r_CATG'y
Cuts at: 3157 3676 4717 5485 5985 6892 3157
Size: 519 1041 768 500 907 4697
Fragments arranged by size:
4697 1041 907 768 519 500
PasI CC'CwG_GG
Cuts at: 1591 1641 2339 2988 3389 3518 4285 1591
Size: 50 698 649 401 129 767 5738
Fragments arranged by size:
5738 767 698 649 401 129 50
PciI A'CATG_T
Cuts at: 6888 6888
Size: 8432
PflMI CCAn_nnn'nTGG
Cuts at: 1066 1488 1930 3099 3988 5247 1066
Size: 422 442 1169 889 1259 4251
Fragments arranged by size:
4251 1259 1169 889 442 422
PfoI T'CCnGG_A
Cuts at: 384 2080 384
Size: 1696 6736
PmeI GTTT'AAAC
Cuts at: 5431 5431
Size: 8432
PmlI CAC'GTG
Cuts at: 125 2442 5114 125
Size: 2317 2672 3443
Fragments arranged by size:
3443 2672 2317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5262 5294 7571 7603 5262
Size: 32 2277 32 6091
Fragments arranged by size:
6091 2277 32 32
PpuMI rG'GwC_Cy
Cuts at: 129 1272 1859 2281 2815 3140 3210 3953 4289
Size: 1143 587 422 534 325 70 743 336
Cuts at: 4289 129
Size: 4272
Fragments arranged by size:
4272 1143 743 587 534 422 336 325
70
PshAI GACnn'nnGTC
Cuts at: 2089 2834 2089
Size: 745 7687
PsiI TTA'TAA
Cuts at: 887 3734 887
Size: 2847 5585
PspOMI G'GGCC_C
Cuts at: 1517 2189 2762 3038 1517
Size: 672 573 276 6911
Fragments arranged by size:
6911 672 573 276
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5110 5142 5110
Size: 32 8400
PstI C_TGCA'G
Cuts at: 291 650 1512 2294 4398 5479 6164 291
Size: 359 862 782 2104 1081 685 2559
Fragments arranged by size:
2559 2104 1081 862 782 685 359
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8432
PvuII CAG'CTG
Cuts at: 3073 5216 5857 6217 3073
Size: 2143 641 360 5288
Fragments arranged by size:
5288 2143 641 360
RsrII CG'GwC_CG
Cuts at: 6627 6627
Size: 8432
SacI G_AGCT'C
Cuts at: 1215 1975 2941 4333 5446 1215
Size: 760 966 1392 1113 4201
Fragments arranged by size:
4201 1392 1113 966 760
SacII CC_GC'GG
Cuts at: 3601 3601
Size: 8432
SalI G'TCGA_C
Cuts at: 1371 2313 5469 1371
Size: 942 3156 4334
Fragments arranged by size:
4334 3156 942
SanDI GG'GwC_CC
Cuts at: 129 2815 3210 3953 129
Size: 2686 395 743 4608
Fragments arranged by size:
4608 2686 743 395
SapI GCTCTTCn'nnn_
Cuts at: 6455 6665 6455
Size: 210 8222
SbfI CC_TGCA'GG
Cuts at: 5479 5479
Size: 8432
ScaI AGT'ACT
Cuts at: 2587 7949 2587
Size: 5362 3070
SfcI C'TryA_G
Cuts at: 33 287 646 684 1076 1508 2290 4394 5199
Size: 254 359 38 392 432 782 2104 805
Cuts at: 5199 5475 6160 7153 7344 33
Size: 276 685 993 191 1121
Fragments arranged by size:
2104 1121 993 805 782 685 432 392
359 276 254 191 38
SfoI GGC'GCC
Cuts at: 1319 1598 1746 4521 5864 6112 1319
Size: 279 148 2775 1343 248 3639
Fragments arranged by size:
3639 2775 1343 279 248 148
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8432
SmaI CCC'GGG
Cuts at: 1408 2473 2813 3208 5454 1408
Size: 1065 340 395 2246 4386
Fragments arranged by size:
4386 2246 1065 395 340
SmlI C'TyrA_G
Cuts at: 1911 2487 3815 4865 5587 6502 6994 7256 1911
Size: 576 1328 1050 722 915 492 262 3087
Fragments arranged by size:
3087 1328 1050 915 722 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8432
SphI G_CATG'C
Cuts at: 3676 4717 5485 5985 3676
Size: 1041 768 500 6123
Fragments arranged by size:
6123 1041 768 500
SspI AAT'ATT
Cuts at: 1301 1301
Size: 8432
StuI AGG'CCT
Cuts at: 4414 4414
Size: 8432
StyI C'CwwG_G
Cuts at: 107 1254 1268 1739 1929 1985 2388 2603 3062
Size: 1147 14 471 190 56 403 215 459
Cuts at: 3062 3422 4292 5088 5566 5920 107
Size: 360 870 796 478 354 2619
Fragments arranged by size:
2619 1147 870 796 478 471 459 403
360 354 215 190 56 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 150 780 1453 1654 1972 2828 3273 3601 4108
Size: 630 673 201 318 856 445 328 507
Cuts at: 4108 4965 6015 6148 6607 6816 150
Size: 857 1050 133 459 209 1766
Fragments arranged by size:
1766 1050 857 856 673 630 507 459
445 328 318 209 201 133
TatI w'GTAC_w
Cuts at: 269 839 2585 7947 269
Size: 570 1746 5362 754
Fragments arranged by size:
5362 1746 754 570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1618 1650 1986 2018 2344 2376 2431 2463 3520
Size: 32 336 32 326 32 55 32 1057
Cuts at: 3520 3552 4185 4217 7949 7981 1618
Size: 32 633 32 3732 32 2069
Fragments arranged by size:
3732 2069 1057 633 336 326 55 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6229 6229
Size: 8432
XbaI T'CTAG_A
Cuts at: 5463 5463
Size: 8432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 3889 3942 5927 156
Size: 3733 53 1985 2661
Fragments arranged by size:
3733 2661 1985 53
XmaI C'CCGG_G
Cuts at: 1406 2471 2811 3206 5452 1406
Size: 1065 340 395 2246 4386
Fragments arranged by size:
4386 2246 1065 395 340
XmnI GAAnn'nnTTC
Cuts at: 711 3344 711
Size: 2633 5799
ZraI GAC'GTC
Cuts at: 4451 4451
Size: 8432
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AlfI AscI AvrII BsgI BsiWI BsrGI BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI PacI PspXI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI