Restriction map for
pF1KA1867
(Circular) (Six-base) MAPSORT of: pF1KA1867.seq Check: 77 from: 1 to: 5447
pF1KA1867 5447 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 622 622
Size: 5447
AatII G_ACGT'C
Cuts at: 829 1100 829
Size: 271 5176
AccI GT'mk_AC
Cuts at: 1100 2409 2485 1100
Size: 1309 76 4062
Fragments arranged by size:
4062 1309 76
Acc65I G'GTAC_C
Cuts at: 2463 2463
Size: 5447
AclI AA'CG_TT
Cuts at: 1481 5287 1481
Size: 3806 1641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 240 463 1152 2017 3272 3704 4451 4719
Size: 102 223 689 865 1255 432 747 268
Cuts at: 4719 138
Size: 866
Fragments arranged by size:
1255 866 865 747 689 432 268 223
102
AflIII A'CryG_T
Cuts at: 1022 1295 2799 3903 4880 1022
Size: 273 1504 1104 977 1589
Fragments arranged by size:
1589 1504 1104 977 273
AgeI A'CCGG_T
Cuts at: 3835 3835
Size: 5447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 1588 488
Size: 1100 4347
AlwNI CAG_nnn'CTG
Cuts at: 405 1699 1870 1995 2752 4319 405
Size: 1294 171 125 757 1567 1533
Fragments arranged by size:
1567 1533 1294 757 171 125
ApaI G_GGCC'C
Cuts at: 574 1112 574
Size: 538 4909
ApaLI G'TGCA_C
Cuts at: 4217 4217
Size: 5447
ApoI r'AATT_y
Cuts at: 1927 2451 4800 5056 1927
Size: 524 2349 256 2318
Fragments arranged by size:
2349 2318 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5447
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5447
AvaI C'yCGr_G
Cuts at: 2467 2467
Size: 5447
BamHI G'GATC_C
Cuts at: 2472 2472
Size: 5447
BanI G'GyrC_C
Cuts at: 540 1311 2463 2877 3125 3160 540
Size: 771 1152 414 248 35 2827
Fragments arranged by size:
2827 1152 771 414 248 35
BanII G_rGCy'C
Cuts at: 574 1040 1112 1156 1225 2461 3491 574
Size: 466 72 44 69 1236 1030 2530
Fragments arranged by size:
2530 1236 1030 466 72 69 44
BbeI G_GCGC'C
Cuts at: 2881 3129 2881
Size: 248 5199
BbsI GAAGACnn'nnnn_
Cuts at: 1255 1255
Size: 5447
BbvCI CC'TCA_GC
Cuts at: 1081 2254 1081
Size: 1173 4274
BciVI GTATCCnnnnn_n'
Cuts at: 3345 4106 5447 3345
Size: 761 1341 3345
Fragments arranged by size:
3345 1341 761
BclI T'GATC_A
Cuts at: 578 2967 578
Size: 2389 3058
BglI GCCn_nnn'nGGC
Cuts at: 499 2350 499
Size: 1851 3596
BglII A'GATC_T
Cuts at: 2962 4999 2962
Size: 2037 3410
BlpI GC'TnA_GC
Cuts at: 2559 2559
Size: 5447
Bme1580I G_kGCm'C
Cuts at: 574 1112 1316 1498 1791 2117 3072 3165 4221
Size: 538 204 182 293 326 955 93 1056
Cuts at: 4221 574
Size: 1800
Fragments arranged by size:
1800 1056 955 538 326 293 204 182
93
BmgBI CAC'GTC
Cuts at: 762 2439 762
Size: 1677 3770
BmrI ACTGGGnnnn_n'
Cuts at: 942 2373 2399 2659 2740 3075 5219 942
Size: 1431 26 260 81 335 2144 1170
Fragments arranged by size:
2144 1431 1170 335 260 81 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1059 1908 2064 2269 3814 1059
Size: 849 156 205 1545 2692
Fragments arranged by size:
2692 1545 849 205 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 913 1013 1359 1507 1978 2055 157
Size: 756 100 346 148 471 77 3549
Fragments arranged by size:
3549 756 471 346 148 100 77
Bpu10I CC'TnA_GC
Cuts at: 1081 1237 2152 2254 1081
Size: 156 915 102 4274
Fragments arranged by size:
4274 915 156 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 1633 2623 3502 3994 4292 372
Size: 1261 990 879 492 298 1527
Fragments arranged by size:
1527 1261 990 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1513 5151 1513
Size: 3638 1809
BsaAI yAC'GTr
Cuts at: 1025 1296 2800 1025
Size: 271 1504 3672
Fragments arranged by size:
3672 1504 271
BsaBI GATnn'nnATC
Cuts at: 6 2961 4998 6
Size: 2955 2037 455
Fragments arranged by size:
2955 2037 455
BsaHI Gr'CG_yC
Cuts at: 826 1097 1541 2878 3126 3828 5324 826
Size: 271 444 1337 248 702 1496 949
Fragments arranged by size:
1496 1337 949 702 444 271 248
BsaWI w'CCGG_w
Cuts at: 1225 1433 2636 3157 3835 4109 4256 4667 1225
Size: 208 1203 521 678 274 147 411 2005
Fragments arranged by size:
2005 1203 678 521 411 274 208 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1727 1757 1969 1999 2251 2281 1727
Size: 30 212 30 252 30 4893
Fragments arranged by size:
4893 252 212 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1549 1900 1990 2355 1549
Size: 351 90 365 4641
Fragments arranged by size:
4641 365 351 90
BseYI C'CCAG_C
Cuts at: 165 198 936 1388 2196 2872 3630 4207 4703
Size: 33 738 452 808 676 758 577 496
Cuts at: 4703 165
Size: 909
Fragments arranged by size:
909 808 758 738 676 577 496 452
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1587 2350 1587
Size: 763 4684
BsiEI CG_ry'CG
Cuts at: 104 2989 3035 4243 104
Size: 2885 46 1208 1308
Fragments arranged by size:
2885 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 437 1441 1892 2045 2461 3239 3429 4221 437
Size: 1004 451 153 416 778 190 792 1663
Fragments arranged by size:
1663 1004 792 778 451 416 190 153
BsmBI CGTCTCn'nnnn_
Cuts at: 817 1047 817
Size: 230 5217
Bsp1286I G_dGCh'C
Cuts at: 437 574 1040 1112 1156 1225 1316 1441 1498
Size: 137 466 72 44 69 91 125 57
Cuts at: 1498 1791 1892 2045 2117 2461 3072 3165 3239
Size: 293 101 153 72 344 611 93 74
Cuts at: 3239 3429 3491 4221 437
Size: 190 62 730 1663
Fragments arranged by size:
1663 730 611 466 344 293 190 153
137 125 101 93 91 74 72 72
69 62 57 44
BspEI T'CCGG_A
Cuts at: 1225 1433 4667 1225
Size: 208 3234 2005
Fragments arranged by size:
3234 2005 208
BspHI T'CATG_A
Cuts at: 4623 4623
Size: 5447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 2096 2497 2711 3013 3394 622
Size: 1474 401 214 302 381 2675
Fragments arranged by size:
2675 1474 401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 512 2156 2232 3739 3793 5277 5442 50
Size: 462 1644 76 1507 54 1484 165 55
Fragments arranged by size:
1644 1507 1484 462 165 76 55 54
BsrDI GCAATG_nn'
Cuts at: 1248 3359 1248
Size: 2111 3336
BsrFI r'CCGG_y
Cuts at: 1183 3445 3626 3835 1183
Size: 2262 181 209 2795
Fragments arranged by size:
2795 2262 209 181
BsrGI T'GTAC_A
Cuts at: 1001 1001
Size: 5447
BssSI C'ACGA_G
Cuts at: 1141 3718 4076 1141
Size: 2577 358 2512
Fragments arranged by size:
2577 2512 358
BstAPI GCAn_nnn'nTGC
Cuts at: 628 2112 2824 628
Size: 1484 712 3251
Fragments arranged by size:
3251 1484 712
BstBI TT'CG_AA
Cuts at: 3808 3808
Size: 5447
BstEII G'GTnAC_C
Cuts at: 831 1053 1341 831
Size: 222 288 4937
Fragments arranged by size:
4937 288 222
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1065
Size: 5447
BstYI r'GATC_y
Cuts at: 1 446 1146 2472 2939 2962 3297 3543 4555
Size: 445 700 1326 467 23 335 246 1012
Cuts at: 4555 4641 4653 4999 1
Size: 86 12 346 449
Fragments arranged by size:
1326 1012 700 467 449 445 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 451 451
Size: 5447
BtgI C'CryG_G
Cuts at: 1401 1401
Size: 5447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1443 3397 3578 3741 1443
Size: 1954 181 163 3149
Fragments arranged by size:
3149 1954 181 163
BtsI GCAGTG_nn'
Cuts at: 915 2290 2690 915
Size: 1375 400 3672
Fragments arranged by size:
3672 1375 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1754 1789 1754
Size: 35 5412
DraI TTT'AAA
Cuts at: 2446 2446
Size: 5447
DraIII CAC_nnn'GTG
Cuts at: 2285 2285
Size: 5447
DrdI GACnn_nn'nnGTC
Cuts at: 904 2296 3153 4011 904
Size: 1392 857 858 2340
Fragments arranged by size:
2340 1392 858 857
EaeI y'GGCC_r
Cuts at: 209 771 1709 1727 2157 3032 3206 3597 3624
Size: 562 938 18 430 875 174 391 27
Cuts at: 3624 4969 209
Size: 1345 687
Fragments arranged by size:
1345 938 875 687 562 430 391 174
27 18
EagI C'GGCC_G
Cuts at: 3032 3032
Size: 5447
EarI CTCTTCn'nnn_
Cuts at: 139 157 172 748 1967 3470 3680 139
Size: 18 15 576 1219 1503 210 1906
Fragments arranged by size:
1906 1503 1219 576 210 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2439 3655 3965 4111 5257 2439
Size: 1216 310 146 1146 2629
Fragments arranged by size:
2629 1216 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2459 2459
Size: 5447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 157 240 463 913 1013 1152 1359 1507
Size: 19 83 223 450 100 139 207 148
Cuts at: 1507 1978 2017 2055 3272 3704 4451 4719 138
Size: 471 39 38 1217 432 747 268 866
Fragments arranged by size:
1217 866 747 471 450 432 268 223
207 148 139 100 83 39 38 19
EcoO109I rG'GnC_Cy
Cuts at: 381 570 571 597 1109 1962 2001 2586 381
Size: 189 1 26 512 853 39 585 3242
Fragments arranged by size:
3242 853 585 512 189 39 26 1
EcoRI G'AATT_C
Cuts at: 1927 2451 1927
Size: 524 4923
EcoRV GAT'ATC
Cuts at: 1836 1836
Size: 5447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 375 3466 375
Size: 3091 2356
FspI TGC'GCA
Cuts at: 3228 3228
Size: 5447
HaeII r_GCGC'y
Cuts at: 1509 2881 3129 4151 5130 1509
Size: 1372 248 1022 979 1826
Fragments arranged by size:
1826 1372 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 659 691 1138 1170 1352 1384 2251 2283 2418
Size: 32 447 32 182 32 867 32 135
Cuts at: 2418 2450 3289 3319 3321 3351 659
Size: 32 839 30 2 30 2755
Fragments arranged by size:
2755 867 839 447 182 135 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 864 1095 2028 2410 2486 864
Size: 231 933 382 76 3825
Fragments arranged by size:
3825 933 382 231 76
KasI G'GCGC_C
Cuts at: 2877 3125 2877
Size: 248 5199
KpnI G_GTAC'C
Cuts at: 2467 2467
Size: 5447
MluI A'CGCG_T
Cuts at: 4880 4880
Size: 5447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1652 4118 4302 1652
Size: 2466 184 2797
Fragments arranged by size:
2797 2466 184
MscI TGG'CCA
Cuts at: 211 773 3208 211
Size: 562 2435 2450
Fragments arranged by size:
2450 2435 562
MslI CAynn'nnrTG
Cuts at: 1723 2122 3436 3563 1723
Size: 399 1314 127 3607
Fragments arranged by size:
3607 1314 399 127
MspA1I CmG'CkG
Cuts at: 940 1172 1908 1956 2075 2187 2309 2421 2558
Size: 232 736 48 119 112 122 112 137
Cuts at: 2558 2872 3232 4245 4490 940
Size: 314 360 1013 245 1897
Fragments arranged by size:
1897 1013 736 360 314 245 232 137
122 119 112 112 48
NaeI GCC'GGC
Cuts at: 3628 3628
Size: 5447
NarI GG'CG_CC
Cuts at: 2878 3126 2878
Size: 248 5199
NgoMIV G'CCGG_C
Cuts at: 3626 3626
Size: 5447
NspI r_CATG'y
Cuts at: 2127 2500 3000 3907 2127
Size: 373 500 907 3667
Fragments arranged by size:
3667 907 500 373
PasI CC'CwG_GG
Cuts at: 1065 1065
Size: 5447
PciI A'CATG_T
Cuts at: 3903 3903
Size: 5447
PflMI CCAn_nnn'nTGG
Cuts at: 282 653 1208 1870 282
Size: 371 555 662 3859
Fragments arranged by size:
3859 662 555 371
PfoI T'CCnGG_A
Cuts at: 1468 2273 1468
Size: 805 4642
PmeI GTTT'AAAC
Cuts at: 2446 2446
Size: 5447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2253 4586 4618 2221
Size: 32 2333 32 3050
Fragments arranged by size:
3050 2333 32 32
PpuMI rG'GwC_Cy
Cuts at: 381 597 1962 2001 381
Size: 216 1365 39 3827
Fragments arranged by size:
3827 1365 216 39
PshAI GACnn'nnGTC
Cuts at: 829 1204 2090 2405 2437 829
Size: 375 886 315 32 3839
Fragments arranged by size:
3839 886 375 315 32
PspOMI G'GGCC_C
Cuts at: 570 1108 570
Size: 538 4909
PstI C_TGCA'G
Cuts at: 642 2420 2494 3179 642
Size: 1778 74 685 2910
Fragments arranged by size:
2910 1778 685 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5447
PvuII CAG'CTG
Cuts at: 940 1172 1908 1956 2075 2872 3232 940
Size: 232 736 48 119 797 360 3155
Fragments arranged by size:
3155 797 736 360 232 119 48
RsrII CG'GwC_CG
Cuts at: 1452 3642 1452
Size: 2190 3257
SacI G_AGCT'C
Cuts at: 2461 2461
Size: 5447
SalI G'TCGA_C
Cuts at: 2408 2484 2408
Size: 76 5371
SanDI GG'GwC_CC
Cuts at: 597 597
Size: 5447
SapI GCTCTTCn'nnn_
Cuts at: 139 3470 3680 139
Size: 3331 210 1906
Fragments arranged by size:
3331 1906 210
SbfI CC_TGCA'GG
Cuts at: 2494 2494
Size: 5447
ScaI AGT'ACT
Cuts at: 4964 4964
Size: 5447
SexAI A'CCwGG_T
Cuts at: 275 404 275
Size: 129 5318
SfcI C'TryA_G
Cuts at: 33 638 2019 2142 2416 2490 3175 4168 4359
Size: 605 1381 123 274 74 685 993 191
Cuts at: 4359 33
Size: 1121
Fragments arranged by size:
1381 1121 993 685 605 274 191 123
74
SfiI GGCCn_nnn'nGGCC
Cuts at: 499 499
Size: 5447
SfoI GGC'GCC
Cuts at: 2879 3127 2879
Size: 248 5199
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5447
SmaI CCC'GGG
Cuts at: 2469 2469
Size: 5447
SmlI C'TyrA_G
Cuts at: 387 1648 2602 3517 4009 4271 387
Size: 1261 954 915 492 262 1563
Fragments arranged by size:
1563 1261 954 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5447
SphI G_CATG'C
Cuts at: 2500 3000 2500
Size: 500 4947
StyI C'CwwG_G
Cuts at: 212 2581 2935 212
Size: 2369 354 2724
Fragments arranged by size:
2724 2369 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 819 961 1396 3030 3163 3622 3831 358
Size: 461 142 435 1634 133 459 209 1974
Fragments arranged by size:
1974 1634 461 459 435 209 142 133
TatI w'GTAC_w
Cuts at: 950 1001 4962 950
Size: 51 3961 1435
Fragments arranged by size:
3961 1435 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 284 316 1727 1759 2269 2301 2365 2397 4964
Size: 32 1411 32 510 32 64 32 2567
Cuts at: 4964 4996 284
Size: 32 735
Fragments arranged by size:
2567 1411 735 510 64 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 556 901 1942 3244 556
Size: 345 1041 1302 2759
Fragments arranged by size:
2759 1302 1041 345
XbaI T'CTAG_A
Cuts at: 2478 2478
Size: 5447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2942 2942
Size: 5447
XmaI C'CCGG_G
Cuts at: 2467 2467
Size: 5447
XmnI GAAnn'nnTTC
Cuts at: 115 1618 115
Size: 1503 3944
ZraI GAC'GTC
Cuts at: 827 1098 827
Size: 271 5176
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BssHII BstZ17I ClaI
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI StuI SwaI
XhoI