Restriction map for
pF1KA1868
(Circular) (Six-base) MAPSORT of: pF1KA1868.seq Check: 3085 from: 1 to: 5108
pF1KA1868 5108 bp
With 182 enzymes: SgfI *
July 31, 2008 16:04 ..
AccI GT'mk_AC
Cuts at: 2146 2146
Size: 5108
Acc65I G'GTAC_C
Cuts at: 2093 2124 2093
Size: 31 5077
AclI AA'CG_TT
Cuts at: 4948 4948
Size: 5108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1222 1393 1522 2933 3365 4112 4380 1090
Size: 132 171 129 1411 432 747 268 1818
Fragments arranged by size:
1818 1411 747 432 268 171 132 129
AflIII A'CryG_T
Cuts at: 1589 2460 3564 4541 1589
Size: 871 1104 977 2156
Fragments arranged by size:
2156 1104 977 871
AgeI A'CCGG_T
Cuts at: 3496 3496
Size: 5108
AhdI GACnn_n'nnGTC
Cuts at: 1518 1518
Size: 5108
AleI CACnn'nnGTG
Cuts at: 1345 1345
Size: 5108
AlwNI CAG_nnn'CTG
Cuts at: 393 756 951 1125 1245 1359 2413 3980 393
Size: 363 195 174 120 114 1054 1567 1521
Fragments arranged by size:
1567 1521 1054 363 195 174 120 114
ApaI G_GGCC'C
Cuts at: 1422 1422
Size: 5108
ApaLI G'TGCA_C
Cuts at: 583 3878 583
Size: 3295 1813
ApoI r'AATT_y
Cuts at: 175 397 1798 1818 2112 4461 4717 175
Size: 222 1401 20 294 2349 256 566
Fragments arranged by size:
2349 1401 566 294 256 222 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
AvaI C'yCGr_G
Cuts at: 2128 2128
Size: 5108
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1651 1684 1651
Size: 33 5075
BamHI G'GATC_C
Cuts at: 2133 2133
Size: 5108
BanI G'GyrC_C
Cuts at: 1014 1442 2093 2124 2538 2786 2821 1014
Size: 428 651 31 414 248 35 3301
Fragments arranged by size:
3301 651 428 414 248 35 31
BanII G_rGCy'C
Cuts at: 1422 1683 2054 2122 3152 1422
Size: 261 371 68 1030 3378
Fragments arranged by size:
3378 1030 371 261 68
BbeI G_GCGC'C
Cuts at: 1446 2542 2790 1446
Size: 1096 248 3764
Fragments arranged by size:
3764 1096 248
BbsI GAAGACnn'nnnn_
Cuts at: 188 1748 2011 188
Size: 1560 263 3285
Fragments arranged by size:
3285 1560 263
BciVI GTATCCnnnnn_n'
Cuts at: 3006 3767 5108 3006
Size: 761 1341 3006
Fragments arranged by size:
3006 1341 761
BclI T'GATC_A
Cuts at: 887 2628 887
Size: 1741 3367
BglI GCCn_nnn'nGGC
Cuts at: 1012 1603 1012
Size: 591 4517
BglII A'GATC_T
Cuts at: 2623 4660 2623
Size: 2037 3071
BlpI GC'TnA_GC
Cuts at: 2220 2220
Size: 5108
Bme1580I G_kGCm'C
Cuts at: 587 1422 2733 2826 3882 587
Size: 835 1311 93 1056 1813
Fragments arranged by size:
1813 1311 1056 835 93
BmgBI CAC'GTC
Cuts at: 1263 1263
Size: 5108
BmrI ACTGGGnnnn_n'
Cuts at: 2092 2320 2401 2736 4880 2092
Size: 228 81 335 2144 2320
Fragments arranged by size:
2320 2144 335 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 5108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 608 889 1175 1381 1555 523
Size: 85 281 286 206 174 4076
Fragments arranged by size:
4076 286 281 206 174 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 2284 3163 3655 3953 543
Size: 1741 879 492 298 1698
Fragments arranged by size:
1741 1698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 308 444 499 1162 4812 308
Size: 136 55 663 3650 604
Fragments arranged by size:
3650 663 604 136 55
BsaAI yAC'GTr
Cuts at: 2461 2461
Size: 5108
BsaBI GATnn'nnATC
Cuts at: 6 2622 4659 6
Size: 2616 2037 455
Fragments arranged by size:
2616 2037 455
BsaHI Gr'CG_yC
Cuts at: 1443 2539 2787 3489 4985 1443
Size: 1096 248 702 1496 1566
Fragments arranged by size:
1566 1496 1096 702 248
BsaWI w'CCGG_w
Cuts at: 453 2297 2818 3496 3770 3917 4328 453
Size: 1844 521 678 274 147 411 1233
Fragments arranged by size:
1844 1233 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 478 1242 1393 358
Size: 120 764 151 4073
Fragments arranged by size:
4073 764 151 120
BseYI C'CCAG_C
Cuts at: 1934 2533 3291 3868 4364 1934
Size: 599 758 577 496 2678
Fragments arranged by size:
2678 758 599 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2053 1231
Size: 822 4286
BsiEI CG_ry'CG
Cuts at: 104 2650 2696 3904 104
Size: 2546 46 1208 1308
Fragments arranged by size:
2546 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 587 1619 1683 2122 2900 3090 3882 350
Size: 237 1032 64 439 778 190 792 1576
Fragments arranged by size:
1576 1032 792 778 439 237 190 64
BsmI GAATG_Cn'
Cuts at: 214 214
Size: 5108
Bsp1286I G_dGCh'C
Cuts at: 350 587 1422 1619 1683 2054 2122 2733 2826
Size: 237 835 197 64 371 68 611 93
Cuts at: 2826 2900 3090 3152 3882 350
Size: 74 190 62 730 1576
Fragments arranged by size:
1576 835 730 611 371 237 197 190
93 74 68 64 62
BspEI T'CCGG_A
Cuts at: 4328 4328
Size: 5108
BspHI T'CATG_A
Cuts at: 126 1556 1988 4284 126
Size: 1430 432 2296 950
Fragments arranged by size:
2296 1430 950 432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2158 2372 2674 3055 2158
Size: 214 302 381 4211
Fragments arranged by size:
4211 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2042 3400 3454 4938 5103 50
Size: 1992 1358 54 1484 165 55
Fragments arranged by size:
1992 1484 1358 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1200 1736 1779 3020 1200
Size: 536 43 1241 3288
Fragments arranged by size:
3288 1241 536 43
BsrFI r'CCGG_y
Cuts at: 3106 3287 3496 3106
Size: 181 209 4718
Fragments arranged by size:
4718 209 181
BsrGI T'GTAC_A
Cuts at: 1685 1685
Size: 5108
BssSI C'ACGA_G
Cuts at: 972 1254 1612 3379 3737 972
Size: 282 358 1767 358 2343
Fragments arranged by size:
2343 1767 358 358 282
BstAPI GCAn_nnn'nTGC
Cuts at: 1125 2485 1125
Size: 1360 3748
BstBI TT'CG_AA
Cuts at: 3469 3469
Size: 5108
BstXI CCAn_nnnn'nTGG
Cuts at: 579 579
Size: 5108
BstYI r'GATC_y
Cuts at: 1 250 288 806 1632 2133 2600 2623 2958
Size: 249 38 518 826 501 467 23 335
Cuts at: 2958 3204 4216 4302 4314 4660 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 826 518 501 467 449 346 335
249 246 86 38 23 12
BtgI C'CryG_G
Cuts at: 107 1970 107
Size: 1863 3245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 3058 3239 3402 1474
Size: 1584 181 163 3180
Fragments arranged by size:
3180 1584 181 163
BtsI GCAGTG_nn'
Cuts at: 2041 2351 2041
Size: 310 4798
DraI TTT'AAA
Cuts at: 597 2107 597
Size: 1510 3598
DrdI GACnn_nn'nnGTC
Cuts at: 2814 3672 2814
Size: 858 4250
EaeI y'GGCC_r
Cuts at: 1280 1641 2079 2693 2867 3258 3285 4630 1280
Size: 361 438 614 174 391 27 1345 1758
Fragments arranged by size:
1758 1345 614 438 391 361 174 27
EagI C'GGCC_G
Cuts at: 2693 2693
Size: 5108
EarI CTCTTCn'nnn_
Cuts at: 610 1820 1874 3131 3341 610
Size: 1210 54 1257 210 2377
Fragments arranged by size:
2377 1257 1210 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 263 910 1777 2028 3316 3626 3772 4918 263
Size: 647 867 251 1288 310 146 1146 453
Fragments arranged by size:
1288 1146 867 647 453 310 251 146
EcoICRI GAG'CTC
Cuts at: 1681 2120 1681
Size: 439 4669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 608 889 1090 1175 1222 1381 1393 1522
Size: 85 281 201 85 47 159 12 129
Cuts at: 1522 1555 2933 3365 4112 4380 523
Size: 33 1378 432 747 268 1251
Fragments arranged by size:
1378 1251 747 432 281 268 201 159
129 85 85 47 33 12
EcoO109I rG'GnC_Cy
Cuts at: 1418 1419 1506 2247 1418
Size: 1 87 741 4279
Fragments arranged by size:
4279 741 87 1
EcoRI G'AATT_C
Cuts at: 397 1798 2112 397
Size: 1401 314 3393
Fragments arranged by size:
3393 1401 314
EcoRV GAT'ATC
Cuts at: 1392 1392
Size: 5108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 3127 345
Size: 2782 2326
FspI TGC'GCA
Cuts at: 2889 2889
Size: 5108
HaeII r_GCGC'y
Cuts at: 1137 1446 2542 2790 3812 4791 1137
Size: 309 1096 248 1022 979 1454
Fragments arranged by size:
1454 1096 1022 979 309 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 361 393 1504 1536 2950 2980 2982
Size: 32 76 32 1111 32 1414 30 2
Cuts at: 2982 3012 253
Size: 30 2349
Fragments arranged by size:
2349 1414 1111 76 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2147 2147
Size: 5108
HindIII A'AGCT_T
Cuts at: 688 1960 688
Size: 1272 3836
KasI G'GCGC_C
Cuts at: 1442 2538 2786 1442
Size: 1096 248 3764
Fragments arranged by size:
3764 1096 248
KpnI G_GTAC'C
Cuts at: 2097 2128 2097
Size: 31 5077
MluI A'CGCG_T
Cuts at: 4541 4541
Size: 5108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 3779 3963 623
Size: 3156 184 1768
Fragments arranged by size:
3156 1768 184
MscI TGG'CCA
Cuts at: 1282 1643 2869 1282
Size: 361 1226 3521
Fragments arranged by size:
3521 1226 361
MslI CAynn'nnrTG
Cuts at: 1345 3097 3224 1345
Size: 1752 127 3229
Fragments arranged by size:
3229 1752 127
MspA1I CmG'CkG
Cuts at: 756 878 1449 2219 2533 2893 3906 4151 756
Size: 122 571 770 314 360 1013 245 1713
Fragments arranged by size:
1713 1013 770 571 360 314 245 122
NaeI GCC'GGC
Cuts at: 3289 3289
Size: 5108
NarI GG'CG_CC
Cuts at: 1443 2539 2787 1443
Size: 1096 248 3764
Fragments arranged by size:
3764 1096 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5108
NgoMIV G'CCGG_C
Cuts at: 3287 3287
Size: 5108
NspI r_CATG'y
Cuts at: 1593 2161 2661 3568 1593
Size: 568 500 907 3133
Fragments arranged by size:
3133 907 568 500
PciI A'CATG_T
Cuts at: 1589 3564 1589
Size: 1975 3133
PflMI CCAn_nnn'nTGG
Cuts at: 393 756 393
Size: 363 4745
PfoI T'CCnGG_A
Cuts at: 608 616 1152 608
Size: 8 536 4564
Fragments arranged by size:
4564 536 8
PmeI GTTT'AAAC
Cuts at: 2107 2107
Size: 5108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 5076
PpuMI rG'GwC_Cy
Cuts at: 1506 1506
Size: 5108
PspOMI G'GGCC_C
Cuts at: 1418 1418
Size: 5108
PstI C_TGCA'G
Cuts at: 1355 1427 1454 2155 2840 1355
Size: 72 27 701 685 3623
Fragments arranged by size:
3623 701 685 72 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5108
PvuII CAG'CTG
Cuts at: 756 2533 2893 756
Size: 1777 360 2971
Fragments arranged by size:
2971 1777 360
RsrII CG'GwC_CG
Cuts at: 3303 3303
Size: 5108
SacI G_AGCT'C
Cuts at: 1683 2122 1683
Size: 439 4669
SalI G'TCGA_C
Cuts at: 2145 2145
Size: 5108
SapI GCTCTTCn'nnn_
Cuts at: 1820 3131 3341 1820
Size: 1311 210 3587
Fragments arranged by size:
3587 1311 210
SbfI CC_TGCA'GG
Cuts at: 2155 2155
Size: 5108
ScaI AGT'ACT
Cuts at: 439 1540 4625 439
Size: 1101 3085 922
Fragments arranged by size:
3085 1101 922
SfcI C'TryA_G
Cuts at: 33 1351 1423 1450 2151 2836 3829 4020 33
Size: 1318 72 27 701 685 993 191 1121
Fragments arranged by size:
1318 1121 993 701 685 191 72 27
SfoI GGC'GCC
Cuts at: 1444 2540 2788 1444
Size: 1096 248 3764
Fragments arranged by size:
3764 1096 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
SmaI CCC'GGG
Cuts at: 2130 2130
Size: 5108
SmlI C'TyrA_G
Cuts at: 522 2263 3178 3670 3932 522
Size: 1741 915 492 262 1698
Fragments arranged by size:
1741 1698 915 492 262
SpeI A'CTAG_T
Cuts at: 67 147 67
Size: 80 5028
SphI G_CATG'C
Cuts at: 2161 2661 2161
Size: 500 4608
StuI AGG'CCT
Cuts at: 978 1610 978
Size: 632 4476
StyI C'CwwG_G
Cuts at: 107 1010 1052 1598 2242 2596 107
Size: 903 42 546 644 354 2619
Fragments arranged by size:
2619 903 644 546 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2691 2824 3283 3492 2691
Size: 133 459 209 4307
Fragments arranged by size:
4307 459 209 133
TatI w'GTAC_w
Cuts at: 437 1274 1538 1685 4623 437
Size: 837 264 147 2938 922
Fragments arranged by size:
2938 922 837 264 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1134 1166 4625 4657 1134
Size: 32 3459 32 1585
Fragments arranged by size:
3459 1585 32 32
Tth111I GACn'n_nGTC
Cuts at: 2905 2905
Size: 5108
XbaI T'CTAG_A
Cuts at: 861 2139 861
Size: 1278 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 829 1260 1977 2603 829
Size: 431 717 626 3334
Fragments arranged by size:
3334 717 626 431
XmaI C'CCGG_G
Cuts at: 2128 2128
Size: 5108
XmnI GAAnn'nnTTC
Cuts at: 325 650 1108 325
Size: 325 458 4325
Fragments arranged by size:
4325 458 325
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI