Restriction map for
pF1KA1877
(Circular) (Six-base) MAPSORT of: pF1KA1877.seq Check: 8886 from: 1 to: 4700
pF1KA1877 4700 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 1202 1202
Size: 4700
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4700
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2957 3310 3972 2525
Size: 432 353 662 3253
Fragments arranged by size:
3253 662 432 353
AflIII A'CryG_T
Cuts at: 659 1090 1463 2052 3852 4133 659
Size: 431 373 589 1800 281 1226
Fragments arranged by size:
1800 1226 589 431 373 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1474 1682 1474
Size: 208 4492
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 730 762 730
Size: 32 4668
AlwNI CAG_nnn'CTG
Cuts at: 345 861 1152 1590 2005 3443 345
Size: 516 291 438 415 1438 1602
Fragments arranged by size:
1602 1438 516 438 415 291
ApaI G_GGCC'C
Cuts at: 408 408
Size: 4700
ApaLI G'TGCA_C
Cuts at: 1459 1657 3538 1459
Size: 198 1881 2621
Fragments arranged by size:
2621 1881 198
ApoI r'AATT_y
Cuts at: 1342 1704 4053 4309 1342
Size: 362 2349 256 1733
Fragments arranged by size:
2349 1733 362 256
AseI AT'TA_AT
Cuts at: 20 1139 20
Size: 1119 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 565 1543 1720 565
Size: 978 177 3545
Fragments arranged by size:
3545 978 177
BamHI G'GATC_C
Cuts at: 1725 1725
Size: 4700
BanI G'GyrC_C
Cuts at: 295 635 904 912 938 945 1716 2130 2378
Size: 340 269 8 26 7 771 414 248
Cuts at: 2378 2413 295
Size: 35 2582
Fragments arranged by size:
2582 771 414 340 269 248 35 26
8 7
BanII G_rGCy'C
Cuts at: 170 311 408 1714 2744 170
Size: 141 97 1306 1030 2126
Fragments arranged by size:
2126 1306 1030 141 97
BbeI G_GCGC'C
Cuts at: 299 916 942 2134 2382 299
Size: 617 26 1192 248 2617
Fragments arranged by size:
2617 1192 617 248 26
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3654 4700 2598
Size: 1056 1046 2598
Fragments arranged by size:
2598 1056 1046
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglI GCCn_nnn'nGGC
Cuts at: 525 525
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 215 844 1812 215
Size: 629 968 3103
Fragments arranged by size:
3103 968 629
Bme1580I G_kGCm'C
Cuts at: 408 855 950 959 1463 1603 1661 2325 2418
Size: 447 95 9 504 140 58 664 93
Cuts at: 2418 3542 408
Size: 1124 1566
Fragments arranged by size:
1566 1124 664 504 447 140 95 93
58 9
BmrI ACTGGGnnnn_n'
Cuts at: 402 1912 1993 2328 4472 402
Size: 1510 81 335 2144 630
Fragments arranged by size:
2144 1510 630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 482 3067 482
Size: 2585 2115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 407 265
Size: 142 4558
Bpu10I CC'TnA_GC
Cuts at: 961 961
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1876 2755 3469 3767 1876
Size: 879 714 298 2809
Fragments arranged by size:
2809 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 241 383 4404 241
Size: 142 4021 537
Fragments arranged by size:
4021 537 142
BsaAI yAC'GTr
Cuts at: 660 1093 1464 2053 660
Size: 433 371 589 3307
Fragments arranged by size:
3307 589 433 371
BsaBI GATnn'nnATC
Cuts at: 6 187 1616 2214 4251 6
Size: 181 1429 598 2037 455
Fragments arranged by size:
2037 1429 598 455 181
BsaHI Gr'CG_yC
Cuts at: 296 913 939 1199 2131 2379 3081 4577 296
Size: 617 26 260 932 248 702 1496 419
Fragments arranged by size:
1496 932 702 617 419 260 248 26
BsaWI w'CCGG_w
Cuts at: 638 1263 1889 2410 3088 3499 3646 3920 638
Size: 625 626 521 678 411 147 274 1418
Fragments arranged by size:
1418 678 626 625 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 630 682 687 160
Size: 470 52 5 4173
Fragments arranged by size:
4173 470 52 5
BseYI C'CCAG_C
Cuts at: 226 360 927 1162 2125 2883 3548 3956 226
Size: 134 567 235 963 758 665 408 970
Fragments arranged by size:
970 963 758 665 567 408 235 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 587 955 1470 1521 1603 1652 289
Size: 298 368 515 51 82 49 3337
Fragments arranged by size:
3337 515 368 298 82 51 49
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3518 104
Size: 2138 46 1230 1286
Fragments arranged by size:
2138 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1463 1661 1714 2492 2682 3542 1463
Size: 198 53 778 190 860 2621
Fragments arranged by size:
2621 860 778 198 190 53
BsmI GAATG_Cn'
Cuts at: 146 146
Size: 4700
BsmBI CGTCTCn'nnnn_
Cuts at: 747 747
Size: 4700
Bsp1286I G_dGCh'C
Cuts at: 170 311 408 855 950 959 1463 1603 1661
Size: 141 97 447 95 9 504 140 58
Cuts at: 1661 1714 2325 2418 2492 2682 2744 3542 170
Size: 53 611 93 74 190 62 798 1328
Fragments arranged by size:
1328 798 611 504 447 190 141 140
97 95 93 74 62 58 53 9
BspHI T'CATG_A
Cuts at: 1056 1436 3132 1056
Size: 380 1696 2624
Fragments arranged by size:
2624 1696 380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1750 1964 2266 2647 401
Size: 1349 214 302 381 2454
Fragments arranged by size:
2454 1349 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4530 4695 50
Size: 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 133 2612 133
Size: 2479 2221
BsrFI r'CCGG_y
Cuts at: 264 364 722 2698 2879 264
Size: 100 358 1976 181 2085
Fragments arranged by size:
2085 1976 358 181 100
BssHII G'CGCG_C
Cuts at: 881 883 881
Size: 2 4698
BssSI C'ACGA_G
Cuts at: 1513 2971 3679 1513
Size: 1458 708 2534
Fragments arranged by size:
2534 1458 708
BstAPI GCAn_nnn'nTGC
Cuts at: 813 2077 813
Size: 1264 3436
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstYI r'GATC_y
Cuts at: 1 1725 2192 2215 2550 2796 3102 3114 3200
Size: 1724 467 23 335 246 306 12 86
Cuts at: 3200 4252 1
Size: 1052 449
Fragments arranged by size:
1724 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 580 822 978 580
Size: 242 156 4302
Fragments arranged by size:
4302 242 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 793 1603 2650 2831 2994 793
Size: 810 1047 181 163 2499
Fragments arranged by size:
2499 1047 810 181 163
BtsI GCAGTG_nn'
Cuts at: 1659 1943 1659
Size: 284 4416
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DraIII CAC_nnn'GTG
Cuts at: 813 813
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3750 2406
Size: 1344 3356
EaeI y'GGCC_r
Cuts at: 2285 2459 2850 2877 4222 2285
Size: 174 391 27 1345 2763
Fragments arranged by size:
2763 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 625 750 1035 2723 2933 625
Size: 125 285 1688 210 2392
Fragments arranged by size:
2392 1688 285 210 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3650 3796 4510 2908
Size: 742 146 714 3098
Fragments arranged by size:
3098 742 714 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 407 2525 2957 3310 3972 265
Size: 142 2118 432 353 662 993
Fragments arranged by size:
2118 993 662 432 353 142
EcoNI CCTnn'n_nnAGG
Cuts at: 772 772
Size: 4700
EcoO109I rG'GnC_Cy
Cuts at: 324 382 710 1218 1839 324
Size: 58 328 508 621 3185
Fragments arranged by size:
3185 621 508 328 58
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4700
FspI TGC'GCA
Cuts at: 988 2481 988
Size: 1493 3207
HaeII r_GCGC'y
Cuts at: 299 346 916 942 1590 2134 2382 3612 4383
Size: 47 570 26 648 544 248 1230 771
Cuts at: 4383 299
Size: 616
Fragments arranged by size:
1230 771 648 616 570 544 248 47
26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2542 2572 2574 2604 2542
Size: 30 2 30 4638
Fragments arranged by size:
4638 30 30 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4700
KasI G'GCGC_C
Cuts at: 295 912 938 2130 2378 295
Size: 617 26 1192 248 2617
Fragments arranged by size:
2617 1192 617 248 26
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 644 3459 3643 644
Size: 2815 184 1701
Fragments arranged by size:
2815 1701 184
MscI TGG'CCA
Cuts at: 2461 2461
Size: 4700
MslI CAynn'nnrTG
Cuts at: 1207 1438 1466 2689 2816 1207
Size: 231 28 1223 127 3091
Fragments arranged by size:
3091 1223 231 127 28
MspA1I CmG'CkG
Cuts at: 214 282 824 858 870 969 980 992 1811
Size: 68 542 34 12 99 11 12 819
Cuts at: 1811 2125 2485 3269 3514 214
Size: 314 360 784 245 1400
Fragments arranged by size:
1400 819 784 542 360 314 245 99
68 34 12 12 11
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 296 913 939 2131 2379 296
Size: 617 26 1192 248 2617
Fragments arranged by size:
2617 1192 617 248 26
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NspI r_CATG'y
Cuts at: 1753 2253 3856 1753
Size: 500 1603 2597
Fragments arranged by size:
2597 1603 500
PciI A'CATG_T
Cuts at: 3852 3852
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 663 1414 663
Size: 751 3949
PfoI T'CCnGG_A
Cuts at: 242 1370 242
Size: 1128 3572
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PmlI CAC'GTG
Cuts at: 660 1093 1464 660
Size: 433 371 3896
Fragments arranged by size:
3896 433 371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 1218 1218
Size: 4700
PspOMI G'GGCC_C
Cuts at: 404 404
Size: 4700
PstI C_TGCA'G
Cuts at: 398 773 1014 1747 2432 398
Size: 375 241 733 685 2666
Fragments arranged by size:
2666 733 685 375 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 858 870 969 992 2125 2485 858
Size: 12 99 23 1133 360 3073
Fragments arranged by size:
3073 1133 360 99 23 12
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SacII CC_GC'GG
Cuts at: 825 981 825
Size: 156 4544
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SanDI GG'GwC_CC
Cuts at: 1218 1218
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 394 769 1010 1743 2428 3396 3587 33
Size: 361 375 241 733 685 968 191 1146
Fragments arranged by size:
1146 968 733 685 375 361 241 191
SfoI GGC'GCC
Cuts at: 297 914 940 2132 2380 297
Size: 617 26 1192 248 2617
Fragments arranged by size:
2617 1192 617 248 26
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 567 1722 567
Size: 1155 3545
SmlI C'TyrA_G
Cuts at: 1855 2770 3484 3746 1855
Size: 915 714 262 2809
Fragments arranged by size:
2809 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
SspI AAT'ATT
Cuts at: 155 155
Size: 4700
StyI C'CwwG_G
Cuts at: 1034 1834 2188 1034
Size: 800 354 3546
Fragments arranged by size:
3546 800 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 374 1571 2283 2416 2875 3084 374
Size: 1197 712 133 459 209 1990
Fragments arranged by size:
1990 1197 712 459 209 133
TatI w'GTAC_w
Cuts at: 692 1520 4215 692
Size: 828 2695 1177
Fragments arranged by size:
2695 1177 828
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4700
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 415 2195 415
Size: 1780 2920
XmaI C'CCGG_G
Cuts at: 565 1720 565
Size: 1155 3545
ZraI GAC'GTC
Cuts at: 1200 1200
Size: 4700
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BspEI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI