Restriction map for
pF1KA1885
(Circular) (Six-base) MAPSORT of: pF1KA1885.seq Check: 9464 from: 1 to: 6302
pF1KA1885 6302 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 321 2133 2829 321
Size: 1812 696 3794
Fragments arranged by size:
3794 1812 696
AatII G_ACGT'C
Cuts at: 2567 2567
Size: 6302
AccI GT'mk_AC
Cuts at: 2567 3053 3340 2567
Size: 486 287 5529
Fragments arranged by size:
5529 486 287
Acc65I G'GTAC_C
Cuts at: 446 3318 446
Size: 2872 3430
AclI AA'CG_TT
Cuts at: 6142 6142
Size: 6302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1555 2016 2711 4127 4559 4912 5574 902
Size: 653 461 695 1416 432 353 662 1630
Fragments arranged by size:
1630 1416 695 662 653 461 432 353
AflIII A'CryG_T
Cuts at: 1260 3654 5454 5735 1260
Size: 2394 1800 281 1827
Fragments arranged by size:
2394 1827 1800 281
AgeI A'CCGG_T
Cuts at: 1409 1409
Size: 6302
AleI CACnn'nnGTG
Cuts at: 1039 1039
Size: 6302
AlwNI CAG_nnn'CTG
Cuts at: 874 1777 2148 2317 2631 2709 2771 2914 3072
Size: 903 371 169 314 78 62 143 158
Cuts at: 3072 3258 3607 5045 874
Size: 186 349 1438 2131
Fragments arranged by size:
2131 1438 903 371 349 314 186 169
158 143 78 62
ApaI G_GGCC'C
Cuts at: 404 1326 1575 1605 2621 3024 404
Size: 922 249 30 1016 403 3682
Fragments arranged by size:
3682 1016 922 403 249 30
ApaLI G'TGCA_C
Cuts at: 5140 5140
Size: 6302
ApoI r'AATT_y
Cuts at: 2172 2395 3306 5655 5911 2172
Size: 223 911 2349 256 2563
Fragments arranged by size:
2563 2349 911 256 223
AseI AT'TA_AT
Cuts at: 20 20
Size: 6302
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6302
AvaI C'yCGr_G
Cuts at: 1421 1574 2504 2819 2888 3322 1421
Size: 153 930 315 69 434 4401
Fragments arranged by size:
4401 930 434 315 153 69
BamHI G'GATC_C
Cuts at: 1842 2234 2450 3327 1842
Size: 392 216 877 4817
Fragments arranged by size:
4817 877 392 216
BanI G'GyrC_C
Cuts at: 446 614 817 2789 2956 3318 3732 3980 4015
Size: 168 203 1972 167 362 414 248 35
Cuts at: 4015 446
Size: 2733
Fragments arranged by size:
2733 1972 414 362 248 203 168 167
35
BanII G_rGCy'C
Cuts at: 209 404 813 1326 1342 1575 1605 1613 2513
Size: 195 409 513 16 233 30 8 900
Cuts at: 2513 2621 2699 2853 2908 2951 3024 3289 3296
Size: 108 78 154 55 43 73 265 7
Cuts at: 3296 3316 4346 209
Size: 20 1030 2165
Fragments arranged by size:
2165 1030 900 513 409 265 233 195
154 108 78 73 55 43 30 20
16 8 7
BbeI G_GCGC'C
Cuts at: 2793 3736 3984 2793
Size: 943 248 5111
Fragments arranged by size:
5111 943 248
BbsI GAAGACnn'nnnn_
Cuts at: 1396 1396
Size: 6302
BbvCI CC'TCA_GC
Cuts at: 154 1778 2414 154
Size: 1624 636 4042
Fragments arranged by size:
4042 1624 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2324 2358 90
Size: 34 2200 34 4034
Fragments arranged by size:
4034 2200 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 544 4200 5256 6302 544
Size: 3656 1056 1046 544
Fragments arranged by size:
3656 1056 1046 544
BclI T'GATC_A
Cuts at: 3822 3822
Size: 6302
BglI GCCn_nnn'nGGC
Cuts at: 2200 2718 2200
Size: 518 5784
BglII A'GATC_T
Cuts at: 791 2351 3817 5854 791
Size: 1560 1466 2037 1239
Fragments arranged by size:
2037 1560 1466 1239
BlpI GC'TnA_GC
Cuts at: 733 3414 733
Size: 2681 3621
Bme1580I G_kGCm'C
Cuts at: 404 1270 1326 1575 1605 2621 2959 3024 3927
Size: 866 56 249 30 1016 338 65 903
Cuts at: 3927 4020 5144 404
Size: 93 1124 1562
Fragments arranged by size:
1562 1124 1016 903 866 338 249 93
65 56 30
BmrI ACTGGGnnnn_n'
Cuts at: 840 3514 3595 3930 6074 840
Size: 2674 81 335 2144 1068
Fragments arranged by size:
2674 2144 1068 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4669 4669
Size: 6302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 354 1157 1274 1456 1627 1855 2593 2824
Size: 53 803 117 182 171 228 738 231
Cuts at: 2824 3193 3235 3257 301
Size: 369 42 22 3346
Fragments arranged by size:
3346 803 738 369 231 228 182 171
117 53 42 22
Bpu10I CC'TnA_GC
Cuts at: 154 1778 2414 2455 2845 154
Size: 1624 636 41 390 3611
Fragments arranged by size:
3611 1624 636 390 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1356 3032 3478 4357 5071 5369 1356
Size: 1676 446 879 714 298 2289
Fragments arranged by size:
2289 1676 879 714 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 511 1906 6006 511
Size: 1395 4100 807
Fragments arranged by size:
4100 1395 807
BsaAI yAC'GTr
Cuts at: 2864 3655 2864
Size: 791 5511
BsaBI GATnn'nnATC
Cuts at: 6 3816 5853 6
Size: 3810 2037 455
Fragments arranged by size:
3810 2037 455
BsaHI Gr'CG_yC
Cuts at: 2564 2790 3733 3981 4683 6179 2564
Size: 226 943 248 702 1496 2687
Fragments arranged by size:
2687 1496 943 702 248 226
BsaWI w'CCGG_w
Cuts at: 617 920 1409 3491 4012 4690 5101 5248 5522
Size: 303 489 2082 521 678 411 147 274
Cuts at: 5522 617
Size: 1397
Fragments arranged by size:
2082 1397 678 521 489 411 303 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 155 185 222 252 292 322 155
Size: 30 37 30 40 30 6135
Fragments arranged by size:
6135 40 37 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 735 855 2421 2431 735
Size: 120 1566 10 4606
Fragments arranged by size:
4606 1566 120 10
BseYI C'CCAG_C
Cuts at: 2009 2740 2764 3017 3727 4485 5150 5558 2009
Size: 731 24 253 710 758 665 408 2753
Fragments arranged by size:
2753 758 731 710 665 408 253 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 2518 2857 547
Size: 1971 339 3992
Fragments arranged by size:
3992 1971 339
BsiEI CG_ry'CG
Cuts at: 104 3844 3890 5120 104
Size: 3740 46 1230 1286
Fragments arranged by size:
3740 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 813 1613 2513 2699 2786 2908 3296 3316 4094
Size: 800 900 186 87 122 388 20 778
Cuts at: 4094 4284 5144 813
Size: 190 860 1971
Fragments arranged by size:
1971 900 860 800 778 388 190 186
122 87 20
BsmI GAATG_Cn'
Cuts at: 2364 2364
Size: 6302
BsmBI CGTCTCn'nnnn_
Cuts at: 1120 1120
Size: 6302
Bsp1286I G_dGCh'C
Cuts at: 209 404 813 1270 1326 1342 1575 1605 1613
Size: 195 409 457 56 16 233 30 8
Cuts at: 1613 2513 2621 2699 2786 2853 2908 2951 2959
Size: 900 108 78 87 67 55 43 8
Cuts at: 2959 3024 3289 3296 3316 3927 4020 4094 4284
Size: 65 265 7 20 611 93 74 190
Cuts at: 4284 4346 5144 209
Size: 62 798 1367
Fragments arranged by size:
1367 900 798 611 457 409 265 233
195 190 108 93 87 78 74 67
65 62 56 55 43 30 20 16
8 8 7
BspHI T'CATG_A
Cuts at: 4734 4734
Size: 6302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 321 1538 2133 2829 3352 3566 3868 4249 321
Size: 1217 595 696 523 214 302 381 2374
Fragments arranged by size:
2374 1217 696 595 523 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2490 2808 4594 4648 6132 6297 50
Size: 2440 318 1786 54 1484 165 55
Fragments arranged by size:
2440 1786 1484 318 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1352 4214 1352
Size: 2862 3440
BsrFI r'CCGG_y
Cuts at: 989 1409 4300 4481 989
Size: 420 2891 181 2810
Fragments arranged by size:
2891 2810 420 181
BssSI C'ACGA_G
Cuts at: 604 4573 5281 604
Size: 3969 708 1625
Fragments arranged by size:
3969 1625 708
BstAPI GCAn_nnn'nTGC
Cuts at: 621 670 2148 2709 3679 621
Size: 49 1478 561 970 3244
Fragments arranged by size:
3244 1478 970 561 49
BstBI TT'CG_AA
Cuts at: 4663 4663
Size: 6302
BstXI CCAn_nnnn'nTGG
Cuts at: 146 1590 146
Size: 1444 4858
BstYI r'GATC_y
Cuts at: 1 214 791 1842 2234 2351 2450 3086 3327
Size: 213 577 1051 392 117 99 636 241
Cuts at: 3327 3794 3817 4152 4398 4704 4716 4802 5854
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5854 1
Size: 449
Fragments arranged by size:
1052 1051 636 577 467 449 392 335
306 246 241 213 117 99 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 341 751 2624 341
Size: 410 1873 4019
Fragments arranged by size:
4019 1873 410
BtgI C'CryG_G
Cuts at: 210 451 851 1076 1484 2054 2280 210
Size: 241 400 225 408 570 226 4232
Fragments arranged by size:
4232 570 408 400 241 226 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4252 4433 4596 4252
Size: 181 163 5958
Fragments arranged by size:
5958 181 163
BtsI GCAGTG_nn'
Cuts at: 772 1283 3545 772
Size: 511 2262 3529
Fragments arranged by size:
3529 2262 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 622 657 622
Size: 35 6267
DraI TTT'AAA
Cuts at: 3301 3301
Size: 6302
DraIII CAC_nnn'GTG
Cuts at: 3037 3037
Size: 6302
DrdI GACnn_nn'nnGTC
Cuts at: 1202 1672 4008 5352 1202
Size: 470 2336 1344 2152
Fragments arranged by size:
2336 2152 1344 470
EaeI y'GGCC_r
Cuts at: 570 587 1002 1151 2192 3887 4061 4452 4479
Size: 17 415 149 1041 1695 174 391 27
Cuts at: 4479 5824 570
Size: 1345 1048
Fragments arranged by size:
1695 1345 1048 1041 415 391 174 149
27 17
EagI C'GGCC_G
Cuts at: 3887 3887
Size: 6302
EarI CTCTTCn'nnn_
Cuts at: 1232 1739 2369 2687 2935 4325 4535 1232
Size: 507 630 318 248 1390 210 2999
Fragments arranged by size:
2999 1390 630 507 318 248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 4510 5252 5398 6112 2647
Size: 1863 742 146 714 2837
Fragments arranged by size:
2837 1863 742 714 146
EcoICRI GAG'CTC
Cuts at: 811 1611 2511 2697 2906 3294 3314 811
Size: 800 900 186 209 388 20 3799
Fragments arranged by size:
3799 900 800 388 209 186 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 354 902 1157 1274 1456 1555 1627 1855
Size: 53 548 255 117 182 99 72 228
Cuts at: 1855 2016 2593 2711 2824 3193 3235 3257 4127
Size: 161 577 118 113 369 42 22 870
Cuts at: 4127 4559 4912 5574 301
Size: 432 353 662 1029
Fragments arranged by size:
1029 870 662 577 548 432 369 353
255 228 182 161 118 117 113 99
72 53 42 22
EcoNI CCTnn'n_nnAGG
Cuts at: 750 754 2932 750
Size: 4 2178 4120
Fragments arranged by size:
4120 2178 4
EcoO109I rG'GnC_Cy
Cuts at: 168 401 645 1322 1455 1467 1571 1601 1602
Size: 233 244 677 133 12 104 30 1
Cuts at: 1602 2006 2617 2618 2628 2925 3069 3155 3441
Size: 404 611 1 10 297 144 86 286
Cuts at: 3441 168
Size: 3029
Fragments arranged by size:
3029 677 611 404 297 286 244 233
144 133 104 86 30 12 10 1
1
EcoRI G'AATT_C
Cuts at: 3306 3306
Size: 6302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4321 4321
Size: 6302
FspI TGC'GCA
Cuts at: 1736 4083 1736
Size: 2347 3955
HaeII r_GCGC'y
Cuts at: 258 2771 2793 2814 3736 3984 5214 5985 258
Size: 2513 22 21 922 248 1230 771 575
Fragments arranged by size:
2513 1230 922 771 575 248 22 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 292 324 847 879 950 982 1278
Size: 32 54 32 523 32 71 32 296
Cuts at: 1278 1310 2108 2140 2255 2287 2407 2439 4144
Size: 32 798 32 115 32 120 32 1705
Cuts at: 4144 4174 4176 4206 206
Size: 30 2 30 2302
Fragments arranged by size:
2302 1705 798 523 296 120 115 71
54 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 939 3341 939
Size: 2402 3900
HindIII A'AGCT_T
Cuts at: 884 3196 884
Size: 2312 3990
KasI G'GCGC_C
Cuts at: 2789 3732 3980 2789
Size: 943 248 5111
Fragments arranged by size:
5111 943 248
KpnI G_GTAC'C
Cuts at: 450 3322 450
Size: 2872 3430
MluI A'CGCG_T
Cuts at: 5735 5735
Size: 6302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 5061 5245 182
Size: 4879 184 1239
Fragments arranged by size:
4879 1239 184
MscI TGG'CCA
Cuts at: 572 589 1004 2194 4063 572
Size: 17 415 1190 1869 2811
Fragments arranged by size:
2811 1869 1190 415 17
MslI CAynn'nnrTG
Cuts at: 979 1039 1996 4291 4418 979
Size: 60 957 2295 127 2863
Fragments arranged by size:
2863 2295 957 127 60
MspA1I CmG'CkG
Cuts at: 804 1556 1686 1798 2025 3213 3258 3413 3727
Size: 752 130 112 227 1188 45 155 314
Cuts at: 3727 4087 4871 5116 804
Size: 360 784 245 1990
Fragments arranged by size:
1990 1188 784 752 360 314 245 227
155 130 112 45
NaeI GCC'GGC
Cuts at: 4483 4483
Size: 6302
NarI GG'CG_CC
Cuts at: 2790 3733 3981 2790
Size: 943 248 5111
Fragments arranged by size:
5111 943 248
NcoI C'CATG_G
Cuts at: 210 851 1484 2054 2280 210
Size: 641 633 570 226 4232
Fragments arranged by size:
4232 641 633 570 226
NgoMIV G'CCGG_C
Cuts at: 4481 4481
Size: 6302
NruI TCG'CGA
Cuts at: 1173 1173
Size: 6302
NspI r_CATG'y
Cuts at: 1264 3355 3855 5458 1264
Size: 2091 500 1603 2108
Fragments arranged by size:
2108 2091 1603 500
PasI CC'CwG_GG
Cuts at: 1896 2094 2613 2952 2961 3280 1896
Size: 198 519 339 9 319 4918
Fragments arranged by size:
4918 519 339 319 198 9
PciI A'CATG_T
Cuts at: 1260 5454 1260
Size: 4194 2108
PflMI CCAn_nnn'nTGG
Cuts at: 1485 1566 1485
Size: 81 6221
PfoI T'CCnGG_A
Cuts at: 226 226
Size: 6302
PmeI GTTT'AAAC
Cuts at: 3301 3301
Size: 6302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4748 4780 4748
Size: 32 6270
PpuMI rG'GwC_Cy
Cuts at: 645 2006 2628 3155 645
Size: 1361 622 527 3792
Fragments arranged by size:
3792 1361 622 527
PspOMI G'GGCC_C
Cuts at: 400 1322 1571 1601 2617 3020 400
Size: 922 249 30 1016 403 3682
Fragments arranged by size:
3682 1016 922 403 249 30
PstI C_TGCA'G
Cuts at: 318 1400 1504 2153 2187 2208 2776 2903 3068
Size: 1082 104 649 34 21 568 127 165
Cuts at: 3068 3349 4034 318
Size: 281 685 2586
Fragments arranged by size:
2586 1082 685 649 568 281 165 127
104 34 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6302
PvuII CAG'CTG
Cuts at: 804 1686 2025 3213 3258 3727 4087 804
Size: 882 339 1188 45 469 360 3019
Fragments arranged by size:
3019 1188 882 469 360 339 45
RsrII CG'GwC_CG
Cuts at: 4497 4497
Size: 6302
SacI G_AGCT'C
Cuts at: 813 1613 2513 2699 2908 3296 3316 813
Size: 800 900 186 209 388 20 3799
Fragments arranged by size:
3799 900 800 388 209 186 20
SalI G'TCGA_C
Cuts at: 3339 3339
Size: 6302
SanDI GG'GwC_CC
Cuts at: 645 2006 645
Size: 1361 4941
SapI GCTCTTCn'nnn_
Cuts at: 2369 2687 4325 4535 2369
Size: 318 1638 210 4136
Fragments arranged by size:
4136 1638 318 210
SbfI CC_TGCA'GG
Cuts at: 3349 3349
Size: 6302
ScaI AGT'ACT
Cuts at: 5819 5819
Size: 6302
SexAI A'CCwGG_T
Cuts at: 1715 1715
Size: 6302
SfcI C'TryA_G
Cuts at: 33 314 509 1396 1500 2149 2183 2204 2772
Size: 281 195 887 104 649 34 21 568
Cuts at: 2772 2899 3064 3345 4030 4998 5189 33
Size: 127 165 281 685 968 191 1146
Fragments arranged by size:
1146 968 887 685 649 568 281 281
195 191 165 127 104 34 21
SfoI GGC'GCC
Cuts at: 2791 3734 3982 2791
Size: 943 248 5111
Fragments arranged by size:
5111 943 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6302
SmaI CCC'GGG
Cuts at: 1423 2821 3324 1423
Size: 1398 503 4401
Fragments arranged by size:
4401 1398 503
SmlI C'TyrA_G
Cuts at: 1335 3047 3457 4372 5086 5348 1335
Size: 1712 410 915 714 262 2289
Fragments arranged by size:
2289 1712 915 714 410 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6302
SphI G_CATG'C
Cuts at: 3355 3855 3355
Size: 500 5802
StuI AGG'CCT
Cuts at: 331 339 745 2721 2938 331
Size: 8 406 1976 217 3695
Fragments arranged by size:
3695 1976 406 217 8
StyI C'CwwG_G
Cuts at: 210 851 1484 1583 2054 2280 3436 3790 210
Size: 641 633 99 471 226 1156 354 2722
Fragments arranged by size:
2722 1156 641 633 471 354 226 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 1746 1971 3885 4018 4477 4686 805
Size: 941 225 1914 133 459 209 2421
Fragments arranged by size:
2421 1914 941 459 225 209 133
TatI w'GTAC_w
Cuts at: 5817 5817
Size: 6302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 155 187 222 254 826 858 1636 1668 5819
Size: 32 35 32 572 32 778 32 4151
Cuts at: 5819 5851 155
Size: 32 606
Fragments arranged by size:
4151 778 606 572 35 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4099 4099
Size: 6302
XbaI T'CTAG_A
Cuts at: 3333 3333
Size: 6302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3797 3797
Size: 6302
XmaI C'CCGG_G
Cuts at: 1421 2819 3322 1421
Size: 1398 503 4401
Fragments arranged by size:
4401 1398 503
ZraI GAC'GTC
Cuts at: 2565 2565
Size: 6302
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BspEI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI