Restriction map for
pF1KA1887
(Circular) (Six-base) MAPSORT of: pF1KA1887.seq Check: 903 from: 1 to: 6122
pF1KA1887 6122 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 475 768 1004 475
Size: 293 236 5593
Fragments arranged by size:
5593 293 236
AccI GT'mk_AC
Cuts at: 2544 3160 2544
Size: 616 5506
Acc65I G'GTAC_C
Cuts at: 3138 3138
Size: 6122
AclI AA'CG_TT
Cuts at: 5962 5962
Size: 6122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 965 1904 1934 2888 3947 4379 4732 5394
Size: 108 939 30 954 1059 432 353 662
Cuts at: 5394 857
Size: 1585
Fragments arranged by size:
1585 1059 954 939 662 432 353 108
30
AfeI AGC'GCT
Cuts at: 187 187
Size: 6122
AflII C'TTAA_G
Cuts at: 2923 2923
Size: 6122
AflIII A'CryG_T
Cuts at: 2791 3474 5274 5555 2791
Size: 683 1800 281 3358
Fragments arranged by size:
3358 1800 683 281
AleI CACnn'nnGTG
Cuts at: 896 896
Size: 6122
AlwNI CAG_nnn'CTG
Cuts at: 649 1686 2584 2644 3427 4865 649
Size: 1037 898 60 783 1438 1906
Fragments arranged by size:
1906 1438 1037 898 783 60
ApaI G_GGCC'C
Cuts at: 154 533 1016 1029 1281 2005 2909 3072 154
Size: 379 483 13 252 724 904 163 3204
Fragments arranged by size:
3204 904 724 483 379 252 163 13
ApaLI G'TGCA_C
Cuts at: 4960 4960
Size: 6122
ApoI r'AATT_y
Cuts at: 2136 3126 5475 5731 2136
Size: 990 2349 256 2527
Fragments arranged by size:
2527 2349 990 256
AseI AT'TA_AT
Cuts at: 20 2711 20
Size: 2691 3431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6122
AvaI C'yCGr_G
Cuts at: 357 927 1274 2172 2256 2551 2732 2902 2934
Size: 570 347 898 84 295 181 170 32
Cuts at: 2934 3041 3065 3142 357
Size: 107 24 77 3337
Fragments arranged by size:
3337 898 570 347 295 181 170 107
84 77 32 24
BamHI G'GATC_C
Cuts at: 2163 2889 3147 2163
Size: 726 258 5138
Fragments arranged by size:
5138 726 258
BanI G'GyrC_C
Cuts at: 819 1050 1155 1469 1528 2329 2928 3138 3552
Size: 231 105 314 59 801 599 210 414
Cuts at: 3552 3800 3835 819
Size: 248 35 3106
Fragments arranged by size:
3106 801 599 414 314 248 231 210
105 59 35
BanII G_rGCy'C
Cuts at: 154 502 533 838 1016 1029 1281 1456 1832
Size: 348 31 305 178 13 252 175 376
Cuts at: 1832 2005 2379 2457 2560 2885 2909 3072 3136
Size: 173 374 78 103 325 24 163 64
Cuts at: 3136 4166 154
Size: 1030 2110
Fragments arranged by size:
2110 1030 376 374 348 325 305 252
178 175 173 163 103 78 64 31
24 13
BbeI G_GCGC'C
Cuts at: 3556 3804 3556
Size: 248 5874
BbsI GAAGACnn'nnnn_
Cuts at: 233 971 2345 233
Size: 738 1374 4010
Fragments arranged by size:
4010 1374 738
BbvCI CC'TCA_GC
Cuts at: 271 1161 1513 2565 271
Size: 890 352 1052 3828
Fragments arranged by size:
3828 1052 890 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2542 2576 2542
Size: 34 6088
BciVI GTATCCnnnnn_n'
Cuts at: 4020 5076 6122 4020
Size: 1056 1046 4020
Fragments arranged by size:
4020 1056 1046
BclI T'GATC_A
Cuts at: 3642 3642
Size: 6122
BglI GCCn_nnn'nGGC
Cuts at: 1887 2589 1887
Size: 702 5420
BglII A'GATC_T
Cuts at: 3637 5674 3637
Size: 2037 4085
BlpI GC'TnA_GC
Cuts at: 2879 3234 2879
Size: 355 5767
Bme1580I G_kGCm'C
Cuts at: 154 533 824 1016 1029 1053 1152 1281 1533
Size: 379 291 192 13 24 99 129 252
Cuts at: 1533 2005 2208 2909 2933 3072 3747 3840 4964
Size: 472 203 701 24 139 675 93 1124
Cuts at: 4964 154
Size: 1312
Fragments arranged by size:
1312 1124 701 675 472 379 291 252
203 192 139 129 99 93 24 24
13
BmrI ACTGGGnnnn_n'
Cuts at: 676 801 1555 1612 1811 2632 2666 3109 3334
Size: 125 754 57 199 821 34 443 225
Cuts at: 3334 3415 3750 5894 676
Size: 81 335 2144 904
Fragments arranged by size:
2144 904 821 754 443 335 225 199
125 81 57 34
BmtI G_CTAG'C
Cuts at: 1070 1070
Size: 6122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 820 1265 1289 4489 820
Size: 445 24 3200 2453
Fragments arranged by size:
3200 2453 445 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 506 725 2214 2393 2494 2504 319
Size: 187 219 1489 179 101 10 3937
Fragments arranged by size:
3937 1489 219 187 179 101 10
Bpu10I CC'TnA_GC
Cuts at: 271 1161 1513 1676 1826 2314 2565 2607 2645
Size: 890 352 163 150 488 251 42 38
Cuts at: 2645 271
Size: 3748
Fragments arranged by size:
3748 890 488 352 251 163 150 42
38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4177 4891 5189 3298
Size: 879 714 298 4231
Fragments arranged by size:
4231 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 454 2072 2292 5826 454
Size: 1618 220 3534 750
Fragments arranged by size:
3534 1618 750 220
BsaAI yAC'GTr
Cuts at: 2794 3475 2794
Size: 681 5441
BsaBI GATnn'nnATC
Cuts at: 6 3636 5673 6
Size: 3630 2037 455
Fragments arranged by size:
3630 2037 455
BsaHI Gr'CG_yC
Cuts at: 3553 3801 4503 5999 3553
Size: 248 702 1496 3676
Fragments arranged by size:
3676 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3311 3832 4510 4921 5068 5342 3311
Size: 521 678 411 147 274 4091
Fragments arranged by size:
4091 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 900 930 1316 1346 2492 2522 900
Size: 30 386 30 1146 30 4500
Fragments arranged by size:
4500 1146 386 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 403 743 830 849 852 872 1164 1418
Size: 144 340 87 19 3 20 292 254
Cuts at: 1418 1434 1823 2256 2428 2447 2705 3114 259
Size: 16 389 433 172 19 258 409 3267
Fragments arranged by size:
3267 433 409 389 340 292 258 254
172 144 87 20 19 19 16 3
BseYI C'CCAG_C
Cuts at: 145 367 1120 1353 1460 1570 1669 1960 2011
Size: 222 753 233 107 110 99 291 51
Cuts at: 2011 2186 2325 2340 2383 3028 3547 4305 4970
Size: 175 139 15 43 645 519 758 665
Cuts at: 4970 5378 145
Size: 408 889
Fragments arranged by size:
889 758 753 665 645 519 408 291
233 222 175 139 110 107 99 51
43 15
BsiEI CG_ry'CG
Cuts at: 104 3664 3710 4940 104
Size: 3560 46 1230 1286
Fragments arranged by size:
3560 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 212 2457 3136 3914 4104 4964 212
Size: 2245 679 778 190 860 1370
Fragments arranged by size:
2245 1370 860 778 679 190
Bsp1286I G_dGCh'C
Cuts at: 154 212 502 533 824 838 1016 1029 1053
Size: 58 290 31 291 14 178 13 24
Cuts at: 1053 1152 1281 1456 1533 1832 2005 2208 2379
Size: 99 129 175 77 299 173 203 171
Cuts at: 2379 2457 2560 2885 2909 2933 3072 3136 3747
Size: 78 103 325 24 24 139 64 611
Cuts at: 3747 3840 3914 4104 4166 4964 154
Size: 93 74 190 62 798 1312
Fragments arranged by size:
1312 798 611 325 299 291 290 203
190 178 175 173 171 139 129 103
99 93 78 77 74 64 62 58
31 24 24 24 14 13
BspHI T'CATG_A
Cuts at: 4554 4554
Size: 6122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 475 768 1004 3172 3386 3688 4069 292
Size: 183 293 236 2168 214 302 381 2345
Fragments arranged by size:
2345 2168 381 302 293 236 214 183
BsrBI CCG'CTC
Cuts at: 50 4414 4468 5952 6117 50
Size: 4364 54 1484 165 55
Fragments arranged by size:
4364 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 126 735 1768 4034 126
Size: 609 1033 2266 2214
Fragments arranged by size:
2266 2214 1033 609
BsrFI r'CCGG_y
Cuts at: 416 2753 4120 4301 416
Size: 2337 1367 181 2237
Fragments arranged by size:
2337 2237 1367 181
BssSI C'ACGA_G
Cuts at: 4393 5101 4393
Size: 708 5414
BstAPI GCAn_nnn'nTGC
Cuts at: 3499 3499
Size: 6122
BstBI TT'CG_AA
Cuts at: 4483 4483
Size: 6122
BstEII G'GTnAC_C
Cuts at: 351 2344 351
Size: 1993 4129
BstXI CCAn_nnnn'nTGG
Cuts at: 1387 2340 1387
Size: 953 5169
BstYI r'GATC_y
Cuts at: 1 808 2026 2163 2513 2889 3147 3614 3637
Size: 807 1218 137 350 376 258 467 23
Cuts at: 3637 3972 4218 4524 4536 4622 5674 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1218 1052 807 467 449 376 350 335
306 258 246 137 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1446 1446
Size: 6122
BtgI C'CryG_G
Cuts at: 1031 2240 2619 1031
Size: 1209 379 4534
Fragments arranged by size:
4534 1209 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 290 4072 4253 4416 290
Size: 3782 181 163 1996
Fragments arranged by size:
3782 1996 181 163
BtsI GCAGTG_nn'
Cuts at: 135 1524 3365 135
Size: 1389 1841 2892
Fragments arranged by size:
2892 1841 1389
DraI TTT'AAA
Cuts at: 2135 3121 2135
Size: 986 5136
DraIII CAC_nnn'GTG
Cuts at: 519 1696 2797 519
Size: 1177 1101 3844
Fragments arranged by size:
3844 1177 1101
DrdI GACnn_nn'nnGTC
Cuts at: 1268 2638 3828 5172 1268
Size: 1370 1190 1344 2218
Fragments arranged by size:
2218 1370 1344 1190
EaeI y'GGCC_r
Cuts at: 158 1088 2418 2766 2961 3078 3707 3881 4272
Size: 930 1330 348 195 117 629 174 391
Cuts at: 4272 4299 5644 158
Size: 27 1345 636
Fragments arranged by size:
1345 1330 930 636 629 391 348 195
174 117 27
EagI C'GGCC_G
Cuts at: 3707 3707
Size: 6122
EarI CTCTTCn'nnn_
Cuts at: 483 892 2414 4145 4355 483
Size: 409 1522 1731 210 2250
Fragments arranged by size:
2250 1731 1522 409 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 435 4330 5072 5218 5932 435
Size: 3895 742 146 714 625
Fragments arranged by size:
3895 742 714 625 146
EcoICRI GAG'CTC
Cuts at: 2455 3134 2455
Size: 679 5443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 506 725 857 965 1904 1934 2214 2393
Size: 187 219 132 108 939 30 280 179
Cuts at: 2393 2494 2504 2888 3947 4379 4732 5394 319
Size: 101 10 384 1059 432 353 662 1047
Fragments arranged by size:
1059 1047 939 662 432 384 353 280
219 187 179 132 108 101 30 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2470 2470
Size: 6122
EcoO109I rG'GnC_Cy
Cuts at: 150 151 281 529 530 586 646 1012 1013
Size: 1 130 248 1 56 60 366 1
Cuts at: 1013 1025 1026 1042 1142 1246 1278 1683 1724
Size: 12 1 16 100 104 32 405 41
Cuts at: 1724 1852 1888 2002 2261 2280 2822 2906 2919
Size: 128 36 114 259 19 542 84 13
Cuts at: 2919 3261 150
Size: 342 3011
Fragments arranged by size:
3011 542 405 366 342 259 248 130
128 114 104 100 84 60 56 41
36 32 19 16 13 12 1 1
1 1
EcoRI G'AATT_C
Cuts at: 3126 3126
Size: 6122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4141 4141
Size: 6122
FspI TGC'GCA
Cuts at: 3903 3903
Size: 6122
HaeII r_GCGC'y
Cuts at: 189 3556 3804 5034 5805 189
Size: 3367 248 1230 771 506
Fragments arranged by size:
3367 1230 771 506 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 820 852 921 953 2155 2187 2762 2794 3035
Size: 32 69 32 1202 32 575 32 241
Cuts at: 3035 3067 3964 3994 3996 4026 820
Size: 32 897 30 2 30 2916
Fragments arranged by size:
2916 1202 897 575 241 69 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3161 3161
Size: 6122
HindIII A'AGCT_T
Cuts at: 1392 1392
Size: 6122
KasI G'GCGC_C
Cuts at: 3552 3800 3552
Size: 248 5874
KpnI G_GTAC'C
Cuts at: 3142 3142
Size: 6122
MluI A'CGCG_T
Cuts at: 5555 5555
Size: 6122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2312 4881 5065 2312
Size: 2569 184 3369
Fragments arranged by size:
3369 2569 184
MscI TGG'CCA
Cuts at: 160 2420 3883 160
Size: 2260 1463 2399
Fragments arranged by size:
2399 2260 1463
MslI CAynn'nnrTG
Cuts at: 896 1385 4111 4238 896
Size: 489 2726 127 2780
Fragments arranged by size:
2780 2726 489 127
MspA1I CmG'CkG
Cuts at: 1087 1546 2011 3049 3233 3547 3907 4691 4936
Size: 459 465 1038 184 314 360 784 245
Cuts at: 4936 1087
Size: 2273
Fragments arranged by size:
2273 1038 784 465 459 360 314 245
184
NaeI GCC'GGC
Cuts at: 2755 4303 2755
Size: 1548 4574
NarI GG'CG_CC
Cuts at: 3553 3801 3553
Size: 248 5874
NcoI C'CATG_G
Cuts at: 2619 2619
Size: 6122
NgoMIV G'CCGG_C
Cuts at: 2753 4301 2753
Size: 1548 4574
NheI G'CTAG_C
Cuts at: 1066 1066
Size: 6122
NspI r_CATG'y
Cuts at: 3175 3675 5278 3175
Size: 500 1603 4019
Fragments arranged by size:
4019 1603 500
PasI CC'CwG_GG
Cuts at: 526 649 1017 1711 2443 526
Size: 123 368 694 732 4205
Fragments arranged by size:
4205 732 694 368 123
PciI A'CATG_T
Cuts at: 5274 5274
Size: 6122
PflMI CCAn_nnn'nTGG
Cuts at: 179 580 2620 179
Size: 401 2040 3681
Fragments arranged by size:
3681 2040 401
PfoI T'CCnGG_A
Cuts at: 483 1310 483
Size: 827 5295
PmeI GTTT'AAAC
Cuts at: 3121 3121
Size: 6122
PmlI CAC'GTG
Cuts at: 2794 2794
Size: 6122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 6090
PpuMI rG'GwC_Cy
Cuts at: 281 586 1852 2822 2919 281
Size: 305 1266 970 97 3484
Fragments arranged by size:
3484 1266 970 305 97
PspOMI G'GGCC_C
Cuts at: 150 529 1012 1025 1277 2001 2905 3068 150
Size: 379 483 13 252 724 904 163 3204
Fragments arranged by size:
3204 904 724 483 379 252 163 13
PstI C_TGCA'G
Cuts at: 1086 2034 2435 3169 3854 1086
Size: 948 401 734 685 3354
Fragments arranged by size:
3354 948 734 685 401
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6122
PvuII CAG'CTG
Cuts at: 1546 2011 3049 3547 3907 1546
Size: 465 1038 498 360 3761
Fragments arranged by size:
3761 1038 498 465 360
RsrII CG'GwC_CG
Cuts at: 4317 4317
Size: 6122
SacI G_AGCT'C
Cuts at: 2457 3136 2457
Size: 679 5443
SalI G'TCGA_C
Cuts at: 3159 3159
Size: 6122
SapI GCTCTTCn'nnn_
Cuts at: 4145 4355 4145
Size: 210 5912
SbfI CC_TGCA'GG
Cuts at: 3169 3169
Size: 6122
ScaI AGT'ACT
Cuts at: 5639 5639
Size: 6122
SexAI A'CCwGG_T
Cuts at: 1854 1854
Size: 6122
SfcI C'TryA_G
Cuts at: 33 894 1082 2030 2431 2500 3035 3165 3850
Size: 861 188 948 401 69 535 130 685
Cuts at: 3850 4818 5009 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 948 861 685 535 401 191
188 130 69
SfoI GGC'GCC
Cuts at: 3554 3802 3554
Size: 248 5874
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6122
SgrAI Cr'CCGG_yG
Cuts at: 416 416
Size: 6122
SmaI CCC'GGG
Cuts at: 359 2258 2734 2904 2936 3067 3144 359
Size: 1899 476 170 32 131 77 3337
Fragments arranged by size:
3337 1899 476 170 131 77 32
SmlI C'TyrA_G
Cuts at: 2923 3277 4192 4906 5168 2923
Size: 354 915 714 262 3877
Fragments arranged by size:
3877 915 714 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6122
SphI G_CATG'C
Cuts at: 3175 3675 3175
Size: 500 5622
StuI AGG'CCT
Cuts at: 628 2592 628
Size: 1964 4158
StyI C'CwwG_G
Cuts at: 245 320 667 721 1091 2619 2885 3020 3256
Size: 75 347 54 370 1528 266 135 236
Cuts at: 3256 3610 245
Size: 354 2757
Fragments arranged by size:
2757 1528 370 354 347 266 236 135
75 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2317 2332 3705 3838 4297 4506 2317
Size: 15 1373 133 459 209 3933
Fragments arranged by size:
3933 1373 459 209 133 15
TatI w'GTAC_w
Cuts at: 5637 5637
Size: 6122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 505 537 1043 1075 1488 1520 2148
Size: 32 174 32 506 32 413 32 628
Cuts at: 2148 2180 2781 2813 5639 5671 299
Size: 32 601 32 2826 32 750
Fragments arranged by size:
2826 750 628 601 506 413 174 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 682 1856 3919 682
Size: 1174 2063 2885
Fragments arranged by size:
2885 2063 1174
XbaI T'CTAG_A
Cuts at: 3153 3153
Size: 6122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1786 3617 1786
Size: 1831 4291
XmaI C'CCGG_G
Cuts at: 357 2256 2732 2902 2934 3065 3142 357
Size: 1899 476 170 32 131 77 3337
Fragments arranged by size:
3337 1899 476 170 131 77 32
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AgeI AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI