Restriction map for
pF1KA1893
(Circular) (Six-base) MAPSORT of: pF1KA1893.seq Check: 7080 from: 1 to: 5507
pF1KA1893 5507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 784 2545 784
Size: 1761 3746
Acc65I G'GTAC_C
Cuts at: 2523 2523
Size: 5507
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 1280 1328 1541 1565 3332 3764 4511 4779
Size: 1140 48 213 24 1767 432 747 268
Cuts at: 4779 140
Size: 868
Fragments arranged by size:
1767 1140 868 747 432 268 213 48
24
AflIII A'CryG_T
Cuts at: 2859 3963 4940 2859
Size: 1104 977 3426
Fragments arranged by size:
3426 1104 977
AgeI A'CCGG_T
Cuts at: 3895 3895
Size: 5507
AhdI GACnn_n'nnGTC
Cuts at: 1482 1616 1815 1482
Size: 134 199 5174
Fragments arranged by size:
5174 199 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1207 1239 1207
Size: 32 5475
AlwNI CAG_nnn'CTG
Cuts at: 499 667 1456 1789 2074 2812 4379 499
Size: 168 789 333 285 738 1567 1627
Fragments arranged by size:
1627 1567 789 738 333 285 168
ApaI G_GGCC'C
Cuts at: 986 986
Size: 5507
ApaLI G'TGCA_C
Cuts at: 4277 4277
Size: 5507
ApoI r'AATT_y
Cuts at: 2511 4860 5116 2511
Size: 2349 256 2902
Fragments arranged by size:
2902 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 987 1866 1986 2320 2457 2527 987
Size: 879 120 334 137 70 3967
Fragments arranged by size:
3967 879 334 137 120 70
BamHI G'GATC_C
Cuts at: 615 687 711 742 807 831 1143 1332 1356
Size: 72 24 31 65 24 312 189 24
Cuts at: 1356 1878 1974 2118 2125 2532 615
Size: 522 96 144 7 407 3590
Fragments arranged by size:
3590 522 407 312 189 144 96 72
65 31 24 24 24 7
BanI G'GyrC_C
Cuts at: 1860 2326 2523 2937 3185 3220 1860
Size: 466 197 414 248 35 4147
Fragments arranged by size:
4147 466 414 248 197 35
BanII G_rGCy'C
Cuts at: 524 884 986 1058 1122 1409 1742 2147 2521
Size: 360 102 72 64 287 333 405 374
Cuts at: 2521 3551 524
Size: 1030 2480
Fragments arranged by size:
2480 1030 405 374 360 333 287 102
72 64
BbeI G_GCGC'C
Cuts at: 2941 3189 2941
Size: 248 5259
BbsI GAAGACnn'nnnn_
Cuts at: 128 1334 1547 1571 2263 128
Size: 1206 213 24 692 3372
Fragments arranged by size:
3372 1206 692 213 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2283 2317 2420 2454 2283
Size: 34 103 34 5336
Fragments arranged by size:
5336 103 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1113 1278 1470 3405 4166 5507 1113
Size: 165 192 1935 761 1341 1113
Fragments arranged by size:
1935 1341 1113 761 192 165
BclI T'GATC_A
Cuts at: 2489 3027 2489
Size: 538 4969
BglII A'GATC_T
Cuts at: 735 951 1308 3022 5059 735
Size: 216 357 1714 2037 1183
Fragments arranged by size:
2037 1714 1183 357 216
BlpI GC'TnA_GC
Cuts at: 1640 2307 2619 1640
Size: 667 312 4528
Fragments arranged by size:
4528 667 312
Bme1580I G_kGCm'C
Cuts at: 986 1010 1865 3132 3225 4281 986
Size: 24 855 1267 93 1056 2212
Fragments arranged by size:
2212 1267 1056 855 93 24
BmgBI CAC'GTC
Cuts at: 482 482
Size: 5507
BmrI ACTGGGnnnn_n'
Cuts at: 323 2719 2800 3135 5279 323
Size: 2396 81 335 2144 551
Fragments arranged by size:
2396 2144 551 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 438 3874 438
Size: 3436 2071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 756 1473 1826 2262 2272 121
Size: 635 717 353 436 10 3356
Fragments arranged by size:
3356 717 635 436 353 10
Bpu10I CC'TnA_GC
Cuts at: 1392 1392
Size: 5507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2683 3562 4054 4352 2683
Size: 879 492 298 3838
Fragments arranged by size:
3838 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1839 2321 5211 1839
Size: 482 2890 2135
Fragments arranged by size:
2890 2135 482
BsaAI yAC'GTr
Cuts at: 2860 2860
Size: 5507
BsaBI GATnn'nnATC
Cuts at: 6 3021 5058 6
Size: 3015 2037 455
Fragments arranged by size:
3015 2037 455
BsaHI Gr'CG_yC
Cuts at: 1263 2938 3186 3888 5384 1263
Size: 1675 248 702 1496 1386
Fragments arranged by size:
1675 1496 1386 702 248
BsaWI w'CCGG_w
Cuts at: 2696 3217 3895 4169 4316 4727 2696
Size: 521 678 274 147 411 3476
Fragments arranged by size:
3476 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 871 901 1104 1134 1231 1261 2156
Size: 30 609 30 203 30 97 30 895
Cuts at: 2156 2186 232
Size: 30 3553
Fragments arranged by size:
3553 895 609 203 97 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 892 1239 2262 2265 892
Size: 347 1023 3 4134
Fragments arranged by size:
4134 1023 347 3
BseYI C'CCAG_C
Cuts at: 251 337 2932 3690 4267 4763 251
Size: 86 2595 758 577 496 995
Fragments arranged by size:
2595 995 758 577 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 2180 254
Size: 1926 3581
BsiEI CG_ry'CG
Cuts at: 104 2399 2430 3049 3095 4303 104
Size: 2295 31 619 46 1208 1308
Fragments arranged by size:
2295 1308 1208 619 46 31
BsiHKAI G_wGCw'C
Cuts at: 772 1393 1409 2521 3299 3489 4281 772
Size: 621 16 1112 778 190 792 1998
Fragments arranged by size:
1998 1112 792 778 621 190 16
Bsp1286I G_dGCh'C
Cuts at: 524 772 884 986 1010 1058 1122 1393 1409
Size: 248 112 102 24 48 64 271 16
Cuts at: 1409 1742 1865 2147 2521 3132 3225 3299 3489
Size: 333 123 282 374 611 93 74 190
Cuts at: 3489 3551 4281 524
Size: 62 730 1750
Fragments arranged by size:
1750 730 611 374 333 282 271 248
190 123 112 102 93 74 64 62
48 24 16
BspEI T'CCGG_A
Cuts at: 4727 4727
Size: 5507
BspHI T'CATG_A
Cuts at: 4683 4683
Size: 5507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 406 1606 1678 2557 2771 3073 3454 406
Size: 1200 72 879 214 302 381 2459
Fragments arranged by size:
2459 1200 879 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 3799 3853 5337 5502 50
Size: 3749 54 1484 165 55
Fragments arranged by size:
3749 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3419 3419
Size: 5507
BsrFI r'CCGG_y
Cuts at: 2394 3505 3686 3895 2394
Size: 1111 181 209 4006
Fragments arranged by size:
4006 1111 209 181
BssHII G'CGCG_C
Cuts at: 2373 2373
Size: 5507
BssSI C'ACGA_G
Cuts at: 3778 4136 3778
Size: 358 5149
BstAPI GCAn_nnn'nTGC
Cuts at: 268 2389 2884 268
Size: 2121 495 2891
Fragments arranged by size:
2891 2121 495
BstBI TT'CG_AA
Cuts at: 3868 3868
Size: 5507
BstXI CCAn_nnnn'nTGG
Cuts at: 1155 1605 1155
Size: 450 5057
BstYI r'GATC_y
Cuts at: 1 615 639 687 711 735 742 759 807
Size: 614 24 48 24 24 7 17 48
Cuts at: 807 831 951 975 999 1143 1167 1308 1332
Size: 24 120 24 24 144 24 141 24
Cuts at: 1332 1356 1665 1878 1974 2118 2125 2532 2999
Size: 24 309 213 96 144 7 407 467
Cuts at: 2999 3022 3357 3603 4615 4701 4713 5059 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 614 467 449 407 346 335 309
246 213 144 144 141 120 96 86
48 48 24 24 24 24 24 24
24 24 24 23 17 12 7 7
BstZ17I GTA'TAC
Cuts at: 785 785
Size: 5507
Bsu36I CC'TnA_GG
Cuts at: 1251 1725 2154 1251
Size: 474 429 4604
Fragments arranged by size:
4604 474 429
BtgI C'CryG_G
Cuts at: 107 2399 2424 107
Size: 2292 25 3190
Fragments arranged by size:
3190 2292 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 113 2343 2750 113
Size: 2230 407 2870
Fragments arranged by size:
2870 2230 407
DraI TTT'AAA
Cuts at: 2506 2506
Size: 5507
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3213 4071 2265
Size: 948 858 3701
Fragments arranged by size:
3701 948 858
EaeI y'GGCC_r
Cuts at: 1275 2396 2427 3092 3266 3657 3684 5029 1275
Size: 1121 31 665 174 391 27 1345 1753
Fragments arranged by size:
1753 1345 1121 665 391 174 31 27
EagI C'GGCC_G
Cuts at: 2396 2427 3092 2396
Size: 31 665 4811
Fragments arranged by size:
4811 665 31
EarI CTCTTCn'nnn_
Cuts at: 3530 3740 3530
Size: 210 5297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1987 2346 3715 4025 4171 5317 1987
Size: 359 1369 310 146 1146 2177
Fragments arranged by size:
2177 1369 1146 359 310 146
EcoICRI GAG'CTC
Cuts at: 1407 2519 1407
Size: 1112 4395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 140 756 1280 1328 1473 1541 1565 1826
Size: 19 616 524 48 145 68 24 261
Cuts at: 1826 2262 2272 3332 3764 4511 4779 121
Size: 436 10 1060 432 747 268 849
Fragments arranged by size:
1060 849 747 616 524 436 432 268
261 145 68 48 24 19 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1134 1491 2277 1134
Size: 357 786 4364
Fragments arranged by size:
4364 786 357
EcoO109I rG'GnC_Cy
Cuts at: 169 347 623 983 1127 1476 1500 1601 1618
Size: 178 276 360 144 349 24 101 17
Cuts at: 1618 1761 1982 2420 2497 2646 169
Size: 143 221 438 77 149 3030
Fragments arranged by size:
3030 438 360 349 276 221 178 149
144 143 101 77 24 17
EcoRI G'AATT_C
Cuts at: 2511 2511
Size: 5507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5507
FspI TGC'GCA
Cuts at: 3288 3288
Size: 5507
HaeII r_GCGC'y
Cuts at: 2051 2941 3189 4211 5190 2051
Size: 890 248 1022 979 2368
Fragments arranged by size:
2368 1022 979 890 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 487 511 519 543 559 591 607
Size: 32 15 24 8 24 16 32 16
Cuts at: 607 639 727 759 799 823 831 855 1015
Size: 32 88 32 40 24 8 24 160
Cuts at: 1015 1047 1104 1136 1152 1159 1184 1191 1231
Size: 32 57 32 16 7 25 7 40
Cuts at: 1231 1263 1300 1324 1332 1356 1396 1420 1428
Size: 32 37 24 8 24 40 24 8
Cuts at: 1428 1452 1468 1492 1500 1524 1585 1602 1617
Size: 24 16 24 8 24 61 17 15
Cuts at: 1617 1634 1657 1689 1801 1833 1870 1902 2110
Size: 17 23 32 112 32 37 32 208
Cuts at: 2110 2142 2158 2190 2206 2238 3349 3379 3381
Size: 32 16 32 16 32 1111 30 2
Cuts at: 3381 3411 440
Size: 30 2536
Fragments arranged by size:
2536 1111 208 160 112 88 61 57
40 40 40 37 37 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 25 24 24 24
24 24 24 24 24 24 24 23
17 17 16 16 16 16 16 16
15 15 8 8 8 8 8 7
7 2
HincII GTy'rAC
Cuts at: 2546 2546
Size: 5507
KasI G'GCGC_C
Cuts at: 2937 3185 2937
Size: 248 5259
KpnI G_GTAC'C
Cuts at: 2527 2527
Size: 5507
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2490 4178 4362 2490
Size: 1688 184 3635
Fragments arranged by size:
3635 1688 184
MscI TGG'CCA
Cuts at: 1277 3268 1277
Size: 1991 3516
MslI CAynn'nnrTG
Cuts at: 3496 3623 3496
Size: 127 5380
MspA1I CmG'CkG
Cuts at: 141 2401 2618 2932 3292 4305 4550 141
Size: 2260 217 314 360 1013 245 1098
Fragments arranged by size:
2260 1098 1013 360 314 245 217
NaeI GCC'GGC
Cuts at: 2396 3688 2396
Size: 1292 4215
NarI GG'CG_CC
Cuts at: 2938 3186 2938
Size: 248 5259
NcoI C'CATG_G
Cuts at: 107 107
Size: 5507
NgoMIV G'CCGG_C
Cuts at: 2394 3686 2394
Size: 1292 4215
NspI r_CATG'y
Cuts at: 1154 2560 3060 3967 1154
Size: 1406 500 907 2694
Fragments arranged by size:
2694 1406 907 500
PasI CC'CwG_GG
Cuts at: 747 1890 1979 2035 2178 747
Size: 1143 89 56 143 4076
Fragments arranged by size:
4076 1143 143 89 56
PciI A'CATG_T
Cuts at: 3963 3963
Size: 5507
PflMI CCAn_nnn'nTGG
Cuts at: 202 202
Size: 5507
PfoI T'CCnGG_A
Cuts at: 699 1416 1842 2121 699
Size: 717 426 279 4085
Fragments arranged by size:
4085 717 426 279
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4646 4678 4646
Size: 32 5475
PpuMI rG'GwC_Cy
Cuts at: 169 623 1127 1476 1500 1601 1618 1761 2420
Size: 454 504 349 24 101 17 143 659
Cuts at: 2420 169
Size: 3256
Fragments arranged by size:
3256 659 504 454 349 143 101 24
17
PspOMI G'GGCC_C
Cuts at: 982 982
Size: 5507
PstI C_TGCA'G
Cuts at: 942 1632 2055 2134 2385 2554 3239 942
Size: 690 423 79 251 169 685 3210
Fragments arranged by size:
3210 690 685 423 251 169 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 141 2932 3292 141
Size: 2791 360 2356
Fragments arranged by size:
2791 2356 360
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 1409 2521 1409
Size: 1112 4395
SacII CC_GC'GG
Cuts at: 2402 2402
Size: 5507
SalI G'TCGA_C
Cuts at: 2544 2544
Size: 5507
SanDI GG'GwC_CC
Cuts at: 2420 2420
Size: 5507
SapI GCTCTTCn'nnn_
Cuts at: 3530 3740 3530
Size: 210 5297
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 5024 5024
Size: 5507
SfcI C'TryA_G
Cuts at: 33 938 1628 1820 2051 2130 2381 2550 3235
Size: 905 690 192 231 79 251 169 685
Cuts at: 3235 4228 4419 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 905 690 685 251 231 192
191 169 79
SfoI GGC'GCC
Cuts at: 2939 3187 2939
Size: 248 5259
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 2529 2529
Size: 5507
SmlI C'TyrA_G
Cuts at: 2662 3577 4069 4331 2662
Size: 915 492 262 3838
Fragments arranged by size:
3838 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5507
SphI G_CATG'C
Cuts at: 2560 3060 2560
Size: 500 5007
StuI AGG'CCT
Cuts at: 2104 2104
Size: 5507
StyI C'CwwG_G
Cuts at: 107 425 1010 1130 1604 2225 2641 2995 107
Size: 318 585 120 474 621 416 354 2619
Fragments arranged by size:
2619 621 585 474 416 354 318 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3090 3223 3682 3891 3090
Size: 133 459 209 4706
Fragments arranged by size:
4706 459 209 133
TatI w'GTAC_w
Cuts at: 5022 5022
Size: 5507
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1372 1404 2156 2188 5024 5056 1372
Size: 32 752 32 2836 32 1823
Fragments arranged by size:
2836 1823 752 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3304 3304
Size: 5507
XbaI T'CTAG_A
Cuts at: 313 1938 2538 313
Size: 1625 600 3282
Fragments arranged by size:
3282 1625 600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 432 3002 375
Size: 57 2570 2880
Fragments arranged by size:
2880 2570 57
XmaI C'CCGG_G
Cuts at: 2527 2527
Size: 5507
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI BstEII ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI