Restriction map for
pF1KA1901
(Circular) (Six-base) MAPSORT of: pF1KA1901.seq Check: 8254 from: 1 to: 6887
pF1KA1901 6887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3925 3925
Size: 6887
Acc65I G'GTAC_C
Cuts at: 3903 3903
Size: 6887
AclI AA'CG_TT
Cuts at: 6727 6727
Size: 6887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 974 1895 1951 2123 2177 2557 2969 3410
Size: 208 921 56 172 54 380 412 441
Cuts at: 3410 4712 5144 5891 6159 766
Size: 1302 432 747 268 1494
Fragments arranged by size:
1494 1302 921 747 441 432 412 380
268 208 172 56 54
AflIII A'CryG_T
Cuts at: 459 3256 4239 5343 6320 459
Size: 2797 983 1104 977 1026
Fragments arranged by size:
2797 1104 1026 983 977
AgeI A'CCGG_T
Cuts at: 5275 5275
Size: 6887
AhdI GACnn_n'nnGTC
Cuts at: 850 2726 850
Size: 1876 5011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3839 3839
Size: 6887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 6855
AlwNI CAG_nnn'CTG
Cuts at: 2536 4192 5759 2536
Size: 1656 1567 3664
Fragments arranged by size:
3664 1656 1567
ApaLI G'TGCA_C
Cuts at: 5657 5657
Size: 6887
ApoI r'AATT_y
Cuts at: 476 928 1213 1541 1809 2778 2810 3192 3709
Size: 452 285 328 268 969 32 382 517
Cuts at: 3709 3770 3891 6240 6496 476
Size: 61 121 2349 256 867
Fragments arranged by size:
2349 969 867 517 452 382 328 285
268 256 121 61 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 6887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
AvaI C'yCGr_G
Cuts at: 1424 1754 2183 2731 2961 3907 1424
Size: 330 429 548 230 946 4404
Fragments arranged by size:
4404 946 548 429 330 230
AvrII C'CTAG_G
Cuts at: 835 835
Size: 6887
BamHI G'GATC_C
Cuts at: 3912 3912
Size: 6887
BanI G'GyrC_C
Cuts at: 3903 4317 4565 4600 3903
Size: 414 248 35 6190
Fragments arranged by size:
6190 414 248 35
BanII G_rGCy'C
Cuts at: 672 3901 4931 672
Size: 3229 1030 2628
Fragments arranged by size:
3229 2628 1030
BbeI G_GCGC'C
Cuts at: 4321 4569 4321
Size: 248 6639
BbsI GAAGACnn'nnnn_
Cuts at: 1519 3398 1519
Size: 1879 5008
BbvCI CC'TCA_GC
Cuts at: 911 911
Size: 6887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1902 1936 1902
Size: 34 6853
BciVI GTATCCnnnnn_n'
Cuts at: 4785 5546 6887 4785
Size: 761 1341 4785
Fragments arranged by size:
4785 1341 761
BclI T'GATC_A
Cuts at: 4407 4407
Size: 6887
BglII A'GATC_T
Cuts at: 2119 3270 4402 6439 2119
Size: 1151 1132 2037 2567
Fragments arranged by size:
2567 2037 1151 1132
BlpI GC'TnA_GC
Cuts at: 3999 3999
Size: 6887
Bme1580I G_kGCm'C
Cuts at: 3585 4512 4605 5661 3585
Size: 927 93 1056 4811
Fragments arranged by size:
4811 1056 927 93
BmrI ACTGGGnnnn_n'
Cuts at: 4099 4180 4515 6659 4099
Size: 81 335 2144 4327
Fragments arranged by size:
4327 2144 335 81
BmtI G_CTAG'C
Cuts at: 1687 1687
Size: 6887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5254 5254
Size: 6887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1181 1571 2672 3706 1124
Size: 57 390 1101 1034 4305
Fragments arranged by size:
4305 1101 1034 390 57
Bpu10I CC'TnA_GC
Cuts at: 911 911
Size: 6887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 2258 3092 4063 4942 5434 5732 204
Size: 2054 834 971 879 492 298 1359
Fragments arranged by size:
2054 1359 971 879 834 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6591 6591
Size: 6887
BsaAI yAC'GTr
Cuts at: 1951 4240 1951
Size: 2289 4598
BsaBI GATnn'nnATC
Cuts at: 6 3563 4401 6438 6
Size: 3557 838 2037 455
Fragments arranged by size:
3557 2037 838 455
BsaHI Gr'CG_yC
Cuts at: 4318 4566 5268 6764 4318
Size: 248 702 1496 4441
Fragments arranged by size:
4441 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3054 4076 4597 5275 5549 5696 6107 3054
Size: 1022 521 678 274 147 411 3834
Fragments arranged by size:
3834 1022 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1389 1419 1389
Size: 30 6857
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 899 899
Size: 6887
BseYI C'CCAG_C
Cuts at: 3568 4312 5070 5647 6143 3568
Size: 744 758 577 496 4312
Fragments arranged by size:
4312 758 744 577 496
BsiEI CG_ry'CG
Cuts at: 104 4429 4475 5683 104
Size: 4325 46 1208 1308
Fragments arranged by size:
4325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3901 4679 4869 5661 3901
Size: 778 190 792 5127
Fragments arranged by size:
5127 792 778 190
BsmI GAATG_Cn'
Cuts at: 3646 3646
Size: 6887
Bsp1286I G_dGCh'C
Cuts at: 672 3585 3901 4512 4605 4679 4869 4931 5661
Size: 2913 316 611 93 74 190 62 730
Cuts at: 5661 672
Size: 1898
Fragments arranged by size:
2913 1898 730 611 316 190 93 74
62
BspEI T'CCGG_A
Cuts at: 6107 6107
Size: 6887
BspHI T'CATG_A
Cuts at: 2328 3744 6063 2328
Size: 1416 2319 3152
Fragments arranged by size:
3152 2319 1416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1573 3937 4151 4453 4834 1573
Size: 2364 214 302 381 3626
Fragments arranged by size:
3626 2364 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5179 5233 6717 6882 50
Size: 5129 54 1484 165 55
Fragments arranged by size:
5129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4799 4799
Size: 6887
BsrFI r'CCGG_y
Cuts at: 4885 5066 5275 4885
Size: 181 209 6497
Fragments arranged by size:
6497 209 181
BsrGI T'GTAC_A
Cuts at: 435 462 697 435
Size: 27 235 6625
Fragments arranged by size:
6625 235 27
BssSI C'ACGA_G
Cuts at: 5158 5516 5158
Size: 358 6529
BstAPI GCAn_nnn'nTGC
Cuts at: 1954 4264 1954
Size: 2310 4577
BstBI TT'CG_AA
Cuts at: 949 5248 949
Size: 4299 2588
BstYI r'GATC_y
Cuts at: 1 366 760 2119 2497 3270 3912 4379 4402
Size: 365 394 1359 378 773 642 467 23
Cuts at: 4402 4737 4983 5995 6081 6093 6439 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1359 1012 773 642 467 449 394 378
365 346 335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4837 5018 5181 4837
Size: 181 163 6543
Fragments arranged by size:
6543 181 163
BtsI GCAGTG_nn'
Cuts at: 1108 1395 3579 4130 1108
Size: 287 2184 551 3865
Fragments arranged by size:
3865 2184 551 287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1827 1862 1827
Size: 35 6852
DraI TTT'AAA
Cuts at: 825 3886 825
Size: 3061 3826
DrdI GACnn_nn'nnGTC
Cuts at: 4593 5451 4593
Size: 858 6029
EaeI y'GGCC_r
Cuts at: 4472 4646 5037 5064 6409 4472
Size: 174 391 27 1345 4950
Fragments arranged by size:
4950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4472 4472
Size: 6887
EarI CTCTTCn'nnn_
Cuts at: 1141 1706 1889 2018 2192 2502 3488 4910 5120
Size: 565 183 129 174 310 986 1422 210
Cuts at: 5120 1141
Size: 2908
Fragments arranged by size:
2908 1422 986 565 310 210 183 174
129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5095 5405 5551 6697 5095
Size: 310 146 1146 5285
Fragments arranged by size:
5285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3899 3899
Size: 6887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 974 1124 1181 1571 1895 1951 2123 2177
Size: 208 150 57 390 324 56 172 54
Cuts at: 2177 2557 2672 2969 3410 3706 4712 5144 5891
Size: 380 115 297 441 296 1006 432 747
Cuts at: 5891 6159 766
Size: 268 1494
Fragments arranged by size:
1494 1006 747 441 432 390 380 324
297 296 268 208 172 150 115 57
56 54
EcoO109I rG'GnC_Cy
Cuts at: 602 2488 3376 4026 602
Size: 1886 888 650 3463
Fragments arranged by size:
3463 1886 888 650
EcoRI G'AATT_C
Cuts at: 1541 3192 3891 1541
Size: 1651 699 4537
Fragments arranged by size:
4537 1651 699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 181 1004 1858 3213 3352 4906 181
Size: 823 854 1355 139 1554 2162
Fragments arranged by size:
2162 1554 1355 854 823 139
FspI TGC'GCA
Cuts at: 4668 4668
Size: 6887
HaeII r_GCGC'y
Cuts at: 1824 4321 4569 5591 6570 1824
Size: 2497 248 1022 979 2141
Fragments arranged by size:
2497 2141 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 832 836 864 868 1389 1421 2712
Size: 32 319 4 28 4 521 32 1291
Cuts at: 2712 2744 2944 2976 3666 3698 4729 4759 4761
Size: 32 200 32 690 32 1031 30 2
Cuts at: 4761 4791 481
Size: 30 2577
Fragments arranged by size:
2577 1291 1031 690 521 319 200 32
32 32 32 32 30 30 28 4
4 2
HincII GTy'rAC
Cuts at: 855 2401 2426 3926 855
Size: 1546 25 1500 3816
Fragments arranged by size:
3816 1546 1500 25
HpaI GTT'AAC
Cuts at: 2426 2426
Size: 6887
KasI G'GCGC_C
Cuts at: 4317 4565 4317
Size: 248 6639
KpnI G_GTAC'C
Cuts at: 3907 3907
Size: 6887
MluI A'CGCG_T
Cuts at: 6320 6320
Size: 6887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1280 2551 5558 5742 1280
Size: 1271 3007 184 2425
Fragments arranged by size:
3007 2425 1271 184
MscI TGG'CCA
Cuts at: 4648 4648
Size: 6887
MslI CAynn'nnrTG
Cuts at: 343 464 1445 1943 2333 3749 3821 4876 5003
Size: 121 981 498 390 1416 72 1055 127
Cuts at: 5003 343
Size: 2227
Fragments arranged by size:
2227 1416 1055 981 498 390 127 121
72
MspA1I CmG'CkG
Cuts at: 3998 4312 4672 5685 5930 3998
Size: 314 360 1013 245 4955
Fragments arranged by size:
4955 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5068 5068
Size: 6887
NarI GG'CG_CC
Cuts at: 4318 4566 4318
Size: 248 6639
NcoI C'CATG_G
Cuts at: 107 107
Size: 6887
NdeI CA'TA_TG
Cuts at: 1769 1769
Size: 6887
NgoMIV G'CCGG_C
Cuts at: 5066 5066
Size: 6887
NheI G'CTAG_C
Cuts at: 1683 1683
Size: 6887
NspI r_CATG'y
Cuts at: 463 712 3260 3940 4440 5347 463
Size: 249 2548 680 500 907 2003
Fragments arranged by size:
2548 2003 907 680 500 249
PciI A'CATG_T
Cuts at: 459 3256 5343 459
Size: 2797 2087 2003
Fragments arranged by size:
2797 2087 2003
PflMI CCAn_nnn'nTGG
Cuts at: 3075 3075
Size: 6887
PfoI T'CCnGG_A
Cuts at: 1560 1560
Size: 6887
PmeI GTTT'AAAC
Cuts at: 3886 3886
Size: 6887
PmlI CAC'GTG
Cuts at: 1951 1951
Size: 6887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6026 6058 6026
Size: 32 6855
PpuMI rG'GwC_Cy
Cuts at: 602 2488 3376 602
Size: 1886 888 4113
Fragments arranged by size:
4113 1886 888
PspXI vC'TCGA_Gb
Cuts at: 1754 1754
Size: 6887
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 6855
PstI C_TGCA'G
Cuts at: 1584 1724 3437 3934 4619 1584
Size: 140 1713 497 685 3852
Fragments arranged by size:
3852 1713 685 497 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6887
PvuII CAG'CTG
Cuts at: 4312 4672 4312
Size: 360 6527
RsrII CG'GwC_CG
Cuts at: 5082 5082
Size: 6887
SacI G_AGCT'C
Cuts at: 3901 3901
Size: 6887
SalI G'TCGA_C
Cuts at: 3924 3924
Size: 6887
SanDI GG'GwC_CC
Cuts at: 602 602
Size: 6887
SapI GCTCTTCn'nnn_
Cuts at: 2018 2502 4910 5120 2018
Size: 484 2408 210 3785
Fragments arranged by size:
3785 2408 484 210
SbfI CC_TGCA'GG
Cuts at: 3934 3934
Size: 6887
ScaI AGT'ACT
Cuts at: 573 2967 6404 573
Size: 2394 3437 1056
Fragments arranged by size:
3437 2394 1056
SexAI A'CCwGG_T
Cuts at: 737 3468 737
Size: 2731 4156
SfcI C'TryA_G
Cuts at: 33 127 176 575 1400 1580 1688 1720 1804
Size: 94 49 399 825 180 108 32 84
Cuts at: 1804 3433 3930 4615 5608 5799 33
Size: 1629 497 685 993 191 1121
Fragments arranged by size:
1629 1121 993 825 685 497 399 191
180 108 94 84 49 32
SfoI GGC'GCC
Cuts at: 4319 4567 4319
Size: 248 6639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
SmaI CCC'GGG
Cuts at: 2185 3909 2185
Size: 1724 5163
SmlI C'TyrA_G
Cuts at: 219 1424 1754 2273 2961 3107 4042 4957 5449
Size: 1205 330 519 688 146 935 915 492
Cuts at: 5449 5711 219
Size: 262 1395
Fragments arranged by size:
1395 1205 935 915 688 519 492 330
262 146
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6887
SphI G_CATG'C
Cuts at: 3940 4440 3940
Size: 500 6387
SspI AAT'ATT
Cuts at: 294 3765 3795 294
Size: 3471 30 3386
Fragments arranged by size:
3471 3386 30
StuI AGG'CCT
Cuts at: 3439 3439
Size: 6887
StyI C'CwwG_G
Cuts at: 107 835 4021 4375 107
Size: 728 3186 354 2619
Fragments arranged by size:
3186 2619 728 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4470 4603 5062 5271 4470
Size: 133 459 209 6086
Fragments arranged by size:
6086 459 209 133
TatI w'GTAC_w
Cuts at: 435 462 531 571 697 1959 2633 2664 2965
Size: 27 69 40 126 1262 674 31 301
Cuts at: 2965 6402 435
Size: 3437 920
Fragments arranged by size:
3437 1262 920 674 301 126 69 40
31 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6404 6436 6404
Size: 32 6855
Tth111I GACn'n_nGTC
Cuts at: 4684 4684
Size: 6887
XbaI T'CTAG_A
Cuts at: 3918 3918
Size: 6887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 4382 2510
Size: 1872 5015
XhoI C'TCGA_G
Cuts at: 1424 1754 2961 1424
Size: 330 1207 5350
Fragments arranged by size:
5350 1207 330
XmaI C'CCGG_G
Cuts at: 2183 3907 2183
Size: 1724 5163
XmnI GAAnn'nnTTC
Cuts at: 899 2243 899
Size: 1344 5543
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI ApaI AscI BaeI
BfrBI BglI BmgBI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NotI NruI NsiI PacI PasI
PshAI PsiI PspOMI SacII SfiI SgrAI SnaBI SrfI
SwaI ZraI