Restriction map for
pF1KA1902
(Circular) (Six-base) MAPSORT of: pF1KA1902.seq Check: 8986 from: 1 to: 6392
pF1KA1902 6392 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1133 1133
Size: 6392
AccI GT'mk_AC
Cuts at: 1504 3430 1504
Size: 1926 4466
Acc65I G'GTAC_C
Cuts at: 3408 3408
Size: 6392
AclI AA'CG_TT
Cuts at: 6232 6232
Size: 6392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2032 2629 2908 2941 4217 4649 5396 5664 2032
Size: 597 279 33 1276 432 747 268 2760
Fragments arranged by size:
2760 1276 747 597 432 279 268 33
AfeI AGC'GCT
Cuts at: 1003 1003
Size: 6392
AflIII A'CryG_T
Cuts at: 3234 3744 4848 5825 3234
Size: 510 1104 977 3801
Fragments arranged by size:
3801 1104 977 510
AgeI A'CCGG_T
Cuts at: 4780 4780
Size: 6392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2515 2954 2515
Size: 439 5953
AlwNI CAG_nnn'CTG
Cuts at: 1840 2502 2536 3697 5264 1840
Size: 662 34 1161 1567 2968
Fragments arranged by size:
2968 1567 1161 662 34
ApaLI G'TGCA_C
Cuts at: 5162 5162
Size: 6392
ApoI r'AATT_y
Cuts at: 391 440 478 738 1110 1135 1553 2063 2089
Size: 49 38 260 372 25 418 510 26
Cuts at: 2089 2570 3396 5745 6001 391
Size: 481 826 2349 256 782
Fragments arranged by size:
2349 826 782 510 481 418 372 260
256 49 38 26 25
AseI AT'TA_AT
Cuts at: 20 1079 20
Size: 1059 5333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6392
AvaI C'yCGr_G
Cuts at: 3412 3412
Size: 6392
BamHI G'GATC_C
Cuts at: 369 3147 3417 369
Size: 2778 270 3344
Fragments arranged by size:
3344 2778 270
BanI G'GyrC_C
Cuts at: 3256 3370 3408 3822 4070 4105 3256
Size: 114 38 414 248 35 5543
Fragments arranged by size:
5543 414 248 114 38 35
BanII G_rGCy'C
Cuts at: 589 1248 2828 3406 4436 589
Size: 659 1580 578 1030 2545
Fragments arranged by size:
2545 1580 1030 659 578
BbeI G_GCGC'C
Cuts at: 3826 4074 3826
Size: 248 6144
BbsI GAAGACnn'nnnn_
Cuts at: 693 1043 2031 2293 3009 3216 693
Size: 350 988 262 716 207 3869
Fragments arranged by size:
3869 988 716 350 262 207
BbvCI CC'TCA_GC
Cuts at: 3079 3079
Size: 6392
BciVI GTATCCnnnnn_n'
Cuts at: 4290 5051 6392 4290
Size: 761 1341 4290
Fragments arranged by size:
4290 1341 761
BclI T'GATC_A
Cuts at: 303 3345 3912 303
Size: 3042 567 2783
Fragments arranged by size:
3042 2783 567
BfrBI ATG'CAT
Cuts at: 2868 2868
Size: 6392
BglII A'GATC_T
Cuts at: 2655 3279 3907 5944 2655
Size: 624 628 2037 3103
Fragments arranged by size:
3103 2037 628 624
BlpI GC'TnA_GC
Cuts at: 3504 3504
Size: 6392
Bme1580I G_kGCm'C
Cuts at: 4017 4110 5166 4017
Size: 93 1056 5243
Fragments arranged by size:
5243 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2010 3604 3685 4020 6164 2010
Size: 1594 81 335 2144 2238
Fragments arranged by size:
2238 2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4759 4759
Size: 6392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 630 2089 2596 2812 220
Size: 410 1459 507 216 3800
Fragments arranged by size:
3800 1459 507 410 216
Bpu10I CC'TnA_GC
Cuts at: 1869 3079 1869
Size: 1210 5182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 2548 2818 3568 4447 4939 5237 1430
Size: 1118 270 750 879 492 298 2585
Fragments arranged by size:
2585 1118 879 750 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 6096 6096
Size: 6392
BsaAI yAC'GTr
Cuts at: 793 3745 793
Size: 2952 3440
BsaBI GATnn'nnATC
Cuts at: 6 2405 2458 3906 5943 6
Size: 2399 53 1448 2037 455
Fragments arranged by size:
2399 2037 1448 455 53
BsaHI Gr'CG_yC
Cuts at: 3823 4071 4773 6269 3823
Size: 248 702 1496 3946
Fragments arranged by size:
3946 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3035 3581 4102 4780 5054 5201 5612 3035
Size: 546 521 678 274 147 411 3815
Fragments arranged by size:
3815 678 546 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 658
Size: 30 6362
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 1682 1778 1787 1790 1814 1817 1853 2498
Size: 1465 96 9 3 24 3 36 645
Cuts at: 2498 2636 3333 217
Size: 138 697 3276
Fragments arranged by size:
3276 1465 697 645 138 96 36 24
9 3 3
BseYI C'CCAG_C
Cuts at: 3817 4575 5152 5648 3817
Size: 758 577 496 4561
Fragments arranged by size:
4561 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 680 1874 616
Size: 64 1194 5134
Fragments arranged by size:
5134 1194 64
BsiEI CG_ry'CG
Cuts at: 104 3934 3980 5188 104
Size: 3830 46 1208 1308
Fragments arranged by size:
3830 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 589 1248 2828 3406 4184 4374 5166 589
Size: 659 1580 578 778 190 792 1815
Fragments arranged by size:
1815 1580 792 778 659 578 190
BsmI GAATG_Cn'
Cuts at: 1278 1991 2317 2492 1278
Size: 713 326 175 5178
Fragments arranged by size:
5178 713 326 175
Bsp1286I G_dGCh'C
Cuts at: 589 1248 2828 3406 4017 4110 4184 4374 4436
Size: 659 1580 578 611 93 74 190 62
Cuts at: 4436 5166 589
Size: 730 1815
Fragments arranged by size:
1815 1580 730 659 611 578 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5612 5612
Size: 6392
BspHI T'CATG_A
Cuts at: 800 1524 2414 5568 800
Size: 724 890 3154 1624
Fragments arranged by size:
3154 1624 890 724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 638 656 1133 1627 2133 3442 3656 3958 4339
Size: 18 477 494 506 1309 214 302 381
Cuts at: 4339 638
Size: 2691
Fragments arranged by size:
2691 1309 506 494 477 381 302 214
18
BsrBI CCG'CTC
Cuts at: 50 680 2370 4684 4738 6222 6387 50
Size: 630 1690 2314 54 1484 165 55
Fragments arranged by size:
2314 1690 1484 630 165 55 54
BsrDI GCAATG_nn'
Cuts at: 712 4304 712
Size: 3592 2800
BsrFI r'CCGG_y
Cuts at: 4390 4571 4780 4390
Size: 181 209 6002
Fragments arranged by size:
6002 209 181
BssSI C'ACGA_G
Cuts at: 4663 5021 4663
Size: 358 6034
BstAPI GCAn_nnn'nTGC
Cuts at: 162 1389 2956 3769 162
Size: 1227 1567 813 2785
Fragments arranged by size:
2785 1567 1227 813
BstBI TT'CG_AA
Cuts at: 4753 4753
Size: 6392
BstXI CCAn_nnnn'nTGG
Cuts at: 1368 1368
Size: 6392
BstYI r'GATC_y
Cuts at: 1 369 1208 2655 3147 3279 3417 3884 3907
Size: 368 839 1447 492 132 138 467 23
Cuts at: 3907 4242 4488 5500 5586 5598 5944 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1447 1012 839 492 467 449 368 346
335 246 138 132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1674 1674
Size: 6392
BtgI C'CryG_G
Cuts at: 107 1616 107
Size: 1509 4883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4342 4523 4686 4342
Size: 181 163 6048
Fragments arranged by size:
6048 181 163
BtsI GCAGTG_nn'
Cuts at: 697 1702 2290 3635 697
Size: 1005 588 1345 3454
Fragments arranged by size:
3454 1345 1005 588
DraI TTT'AAA
Cuts at: 978 2078 2640 3391 978
Size: 1100 562 751 3979
Fragments arranged by size:
3979 1100 751 562
DraIII CAC_nnn'GTG
Cuts at: 162 2742 162
Size: 2580 3812
DrdI GACnn_nn'nnGTC
Cuts at: 4098 4956 4098
Size: 858 5534
EaeI y'GGCC_r
Cuts at: 3977 4151 4542 4569 5914 3977
Size: 174 391 27 1345 4455
Fragments arranged by size:
4455 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3977 3977
Size: 6392
EarI CTCTTCn'nnn_
Cuts at: 3059 4415 4625 3059
Size: 1356 210 4826
Fragments arranged by size:
4826 1356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4600 4910 5056 6202 4600
Size: 310 146 1146 4790
Fragments arranged by size:
4790 1146 310 146
EcoICRI GAG'CTC
Cuts at: 587 1246 2826 3404 587
Size: 659 1580 578 3575
Fragments arranged by size:
3575 1580 659 578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 630 2032 2089 2596 2629 2812 2908 2941
Size: 410 1402 57 507 33 183 96 33
Cuts at: 2941 4217 4649 5396 5664 220
Size: 1276 432 747 268 948
Fragments arranged by size:
1402 1276 948 747 507 432 410 268
183 96 57 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 612 1284 1832 612
Size: 672 548 5172
Fragments arranged by size:
5172 672 548
EcoO109I rG'GnC_Cy
Cuts at: 627 1651 1678 1837 2122 3531 627
Size: 1024 27 159 285 1409 3488
Fragments arranged by size:
3488 1409 1024 285 159 27
EcoRI G'AATT_C
Cuts at: 2063 3396 2063
Size: 1333 5059
EcoRV GAT'ATC
Cuts at: 1578 1578
Size: 6392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4411 4411
Size: 6392
FspI TGC'GCA
Cuts at: 4173 4173
Size: 6392
HaeII r_GCGC'y
Cuts at: 1005 3339 3826 4074 5096 6075 1005
Size: 2334 487 248 1022 979 1322
Fragments arranged by size:
2334 1322 1022 979 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 200 232 288 320 2490 2522 2640 2659 2672
Size: 32 56 32 2170 32 118 19 13
Cuts at: 2672 2691 3139 3171 4234 4264 4266 4296 200
Size: 19 448 32 1063 30 2 30 2296
Fragments arranged by size:
2296 2170 1063 448 118 56 32 32
32 32 30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 2678 3431 2678
Size: 753 5639
HindIII A'AGCT_T
Cuts at: 1195 1195
Size: 6392
KasI G'GCGC_C
Cuts at: 3822 4070 3822
Size: 248 6144
KpnI G_GTAC'C
Cuts at: 3412 3412
Size: 6392
MluI A'CGCG_T
Cuts at: 5825 5825
Size: 6392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5063 5247 5063
Size: 184 6208
MscI TGG'CCA
Cuts at: 4153 4153
Size: 6392
MslI CAynn'nnrTG
Cuts at: 829 2497 3239 4381 4508 829
Size: 1668 742 1142 127 2713
Fragments arranged by size:
2713 1668 1142 742 127
MspA1I CmG'CkG
Cuts at: 376 1899 1948 3503 3817 4177 5190 5435 376
Size: 1523 49 1555 314 360 1013 245 1333
Fragments arranged by size:
1555 1523 1333 1013 360 314 245 49
NaeI GCC'GGC
Cuts at: 4573 4573
Size: 6392
NarI GG'CG_CC
Cuts at: 3823 4071 3823
Size: 248 6144
NcoI C'CATG_G
Cuts at: 107 1616 107
Size: 1509 4883
NgoMIV G'CCGG_C
Cuts at: 4571 4571
Size: 6392
NsiI A_TGCA'T
Cuts at: 2870 2870
Size: 6392
NspI r_CATG'y
Cuts at: 775 2521 3238 3445 3945 4852 775
Size: 1746 717 207 500 907 2315
Fragments arranged by size:
2315 1746 907 717 500 207
PciI A'CATG_T
Cuts at: 3234 4848 3234
Size: 1614 4778
PmeI GTTT'AAAC
Cuts at: 3391 3391
Size: 6392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 5531 5563 3086
Size: 32 2413 32 3915
Fragments arranged by size:
3915 2413 32 32
PpuMI rG'GwC_Cy
Cuts at: 627 1651 1678 2122 627
Size: 1024 27 444 4897
Fragments arranged by size:
4897 1024 444 27
PsiI TTA'TAA
Cuts at: 2756 2756
Size: 6392
PstI C_TGCA'G
Cuts at: 1130 2790 2933 3439 4124 1130
Size: 1660 143 506 685 3398
Fragments arranged by size:
3398 1660 685 506 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6392
PvuII CAG'CTG
Cuts at: 376 1948 3817 4177 376
Size: 1572 1869 360 2591
Fragments arranged by size:
2591 1869 1572 360
RsrII CG'GwC_CG
Cuts at: 4587 4587
Size: 6392
SacI G_AGCT'C
Cuts at: 589 1248 2828 3406 589
Size: 659 1580 578 3575
Fragments arranged by size:
3575 1580 659 578
SalI G'TCGA_C
Cuts at: 3429 3429
Size: 6392
SanDI GG'GwC_CC
Cuts at: 1651 1651
Size: 6392
SapI GCTCTTCn'nnn_
Cuts at: 4415 4625 4415
Size: 210 6182
SbfI CC_TGCA'GG
Cuts at: 3439 3439
Size: 6392
ScaI AGT'ACT
Cuts at: 576 5909 576
Size: 5333 1059
SfcI C'TryA_G
Cuts at: 33 1126 2786 2929 3435 4120 5113 5304 33
Size: 1093 1660 143 506 685 993 191 1121
Fragments arranged by size:
1660 1121 1093 993 685 506 191 143
SfoI GGC'GCC
Cuts at: 3824 4072 3824
Size: 248 6144
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6392
SmaI CCC'GGG
Cuts at: 3414 3414
Size: 6392
SmlI C'TyrA_G
Cuts at: 1445 2563 2797 3547 4462 4954 5216 1445
Size: 1118 234 750 915 492 262 2621
Fragments arranged by size:
2621 1118 915 750 492 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6392
SphI G_CATG'C
Cuts at: 3445 3945 3445
Size: 500 5892
SspI AAT'ATT
Cuts at: 1083 2102 1083
Size: 1019 5373
StuI AGG'CCT
Cuts at: 1151 1151
Size: 6392
StyI C'CwwG_G
Cuts at: 107 1280 1616 2118 2592 3526 3880 107
Size: 1173 336 502 474 934 354 2619
Fragments arranged by size:
2619 1173 934 502 474 354 336
SwaI ATTT'AAAT
Cuts at: 2078 2078
Size: 6392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 1932 3975 4108 4567 4776 157
Size: 1775 2043 133 459 209 1773
Fragments arranged by size:
2043 1775 1773 459 209 133
TatI w'GTAC_w
Cuts at: 574 2858 5907 574
Size: 2284 3049 1059
Fragments arranged by size:
3049 2284 1059
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2695 2727 2728 2760 5909 5941 2695
Size: 32 1 32 3149 32 3146
Fragments arranged by size:
3149 3146 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 4189 4189
Size: 6392
XbaI T'CTAG_A
Cuts at: 3423 3423
Size: 6392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1149 1997 3887 1149
Size: 848 1890 3654
Fragments arranged by size:
3654 1890 848
XmaI C'CCGG_G
Cuts at: 3412 3412
Size: 6392
XmnI GAAnn'nnTTC
Cuts at: 391 690 1490 1911 2492 2893 391
Size: 299 800 421 581 401 3890
Fragments arranged by size:
3890 800 581 421 401 299
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI XhoI ZraI