Restriction map for
pF1KA1910
(Circular) (Six-base) MAPSORT of: pF1KA1910.seq Check: 3188 from: 1 to: 5201
pF1KA1910 5201 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 139 139
Size: 5201
AccI GT'mk_AC
Cuts at: 227 2239 227
Size: 2012 3189
Acc65I G'GTAC_C
Cuts at: 2217 2217
Size: 5201
AclI AA'CG_TT
Cuts at: 161 5041 161
Size: 4880 321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3458 4205 4473 3026
Size: 432 747 268 3754
Fragments arranged by size:
3754 747 432 268
AflIII A'CryG_T
Cuts at: 222 623 1574 2553 3657 4634 222
Size: 401 951 979 1104 977 789
Fragments arranged by size:
1104 979 977 951 789 401
AgeI A'CCGG_T
Cuts at: 3589 3589
Size: 5201
AhdI GACnn_n'nnGTC
Cuts at: 1419 1419
Size: 5201
AleI CACnn'nnGTG
Cuts at: 1717 2011 1717
Size: 294 4907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1735 1735
Size: 5201
AlwNI CAG_nnn'CTG
Cuts at: 1015 2506 4073 1015
Size: 1491 1567 2143
Fragments arranged by size:
2143 1567 1491
ApaI G_GGCC'C
Cuts at: 2157 2157
Size: 5201
ApaLI G'TGCA_C
Cuts at: 3971 3971
Size: 5201
ApoI r'AATT_y
Cuts at: 1432 2205 4554 4810 1432
Size: 773 2349 256 1823
Fragments arranged by size:
2349 1823 773 256
AseI AT'TA_AT
Cuts at: 20 2090 20
Size: 2070 3131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5201
AvaI C'yCGr_G
Cuts at: 546 1424 1497 1971 2221 546
Size: 878 73 474 250 3526
Fragments arranged by size:
3526 878 474 250 73
BamHI G'GATC_C
Cuts at: 1529 2226 1529
Size: 697 4504
BanI G'GyrC_C
Cuts at: 924 2217 2631 2879 2914 924
Size: 1293 414 248 35 3211
Fragments arranged by size:
3211 1293 414 248 35
BanII G_rGCy'C
Cuts at: 2157 2215 3245 2157
Size: 58 1030 4113
Fragments arranged by size:
4113 1030 58
BbeI G_GCGC'C
Cuts at: 928 2635 2883 928
Size: 1707 248 3246
Fragments arranged by size:
3246 1707 248
BbvCI CC'TCA_GC
Cuts at: 1555 1844 1555
Size: 289 4912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 809 843 90
Size: 34 685 34 4448
Fragments arranged by size:
4448 685 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3099 3860 5201 3099
Size: 761 1341 3099
Fragments arranged by size:
3099 1341 761
BclI T'GATC_A
Cuts at: 2721 2721
Size: 5201
BglI GCCn_nnn'nGGC
Cuts at: 1136 1136
Size: 5201
BglII A'GATC_T
Cuts at: 188 1835 2716 4753 188
Size: 1647 881 2037 636
Fragments arranged by size:
2037 1647 881 636
BlpI GC'TnA_GC
Cuts at: 2313 2313
Size: 5201
Bme1580I G_kGCm'C
Cuts at: 641 1504 2157 2826 2919 3975 641
Size: 863 653 669 93 1056 1867
Fragments arranged by size:
1867 1056 863 669 653 93
BmgBI CAC'GTC
Cuts at: 1575 1575
Size: 5201
BmrI ACTGGGnnnn_n'
Cuts at: 276 1907 2413 2494 2829 4973 276
Size: 1631 506 81 335 2144 504
Fragments arranged by size:
2144 1631 506 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3568 3568
Size: 5201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 496 595 1080 151
Size: 345 99 485 4272
Fragments arranged by size:
4272 485 345 99
Bpu10I CC'TnA_GC
Cuts at: 1110 1555 1844 1110
Size: 445 289 4467
Fragments arranged by size:
4467 445 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2377 3256 3748 4046 1759
Size: 618 879 492 298 2914
Fragments arranged by size:
2914 879 618 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 937 1593 1903 4905 937
Size: 656 310 3002 1233
Fragments arranged by size:
3002 1233 656 310
BsaAI yAC'GTr
Cuts at: 225 2554 225
Size: 2329 2872
BsaBI GATnn'nnATC
Cuts at: 6 1060 2715 4752 6
Size: 1054 1655 2037 455
Fragments arranged by size:
2037 1655 1054 455
BsaHI Gr'CG_yC
Cuts at: 136 925 1786 2632 2880 3582 5078 136
Size: 789 861 846 248 702 1496 259
Fragments arranged by size:
1496 861 846 789 702 259 248
BsaWI w'CCGG_w
Cuts at: 2390 2911 3589 3863 4010 4421 2390
Size: 521 678 274 147 411 3170
Fragments arranged by size:
3170 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 936 966 1912 1942 936
Size: 30 946 30 4195
Fragments arranged by size:
4195 946 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 1810 2064 706
Size: 1104 254 3843
Fragments arranged by size:
3843 1104 254
BseYI C'CCAG_C
Cuts at: 1582 2626 3384 3961 4457 1582
Size: 1044 758 577 496 2326
Fragments arranged by size:
2326 1044 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 738 1258 1594 1695 424
Size: 314 520 336 101 3930
Fragments arranged by size:
3930 520 336 314 101
BsiEI CG_ry'CG
Cuts at: 104 819 2743 2789 3997 104
Size: 715 1924 46 1208 1308
Fragments arranged by size:
1924 1308 1208 715 46
BsiHKAI G_wGCw'C
Cuts at: 584 1708 2215 2993 3183 3975 584
Size: 1124 507 778 190 792 1810
Fragments arranged by size:
1810 1124 792 778 507 190
BsmI GAATG_Cn'
Cuts at: 810 810
Size: 5201
BsmBI CGTCTCn'nnnn_
Cuts at: 127 143 1777 2052 127
Size: 16 1634 275 3276
Fragments arranged by size:
3276 1634 275 16
Bsp1286I G_dGCh'C
Cuts at: 584 641 1504 1708 2157 2215 2826 2919 2993
Size: 57 863 204 449 58 611 93 74
Cuts at: 2993 3183 3245 3975 584
Size: 190 62 730 1810
Fragments arranged by size:
1810 863 730 611 449 204 190 93
74 62 58 57
BspEI T'CCGG_A
Cuts at: 4421 4421
Size: 5201
BspHI T'CATG_A
Cuts at: 4377 4377
Size: 5201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 624 1559 2251 2465 2767 3148 624
Size: 935 692 214 302 381 2677
Fragments arranged by size:
2677 935 692 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3493 3547 5031 5196 50
Size: 3443 54 1484 165 55
Fragments arranged by size:
3443 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 207 3113 207
Size: 2906 2295
BsrFI r'CCGG_y
Cuts at: 1788 3199 3380 3589 1788
Size: 1411 181 209 3400
Fragments arranged by size:
3400 1411 209 181
BssSI C'ACGA_G
Cuts at: 2023 3472 3830 2023
Size: 1449 358 3394
Fragments arranged by size:
3394 1449 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2578 2578
Size: 5201
BstBI TT'CG_AA
Cuts at: 471 3562 471
Size: 3091 2110
BstXI CCAn_nnnn'nTGG
Cuts at: 1049 1049
Size: 5201
BstYI r'GATC_y
Cuts at: 1 188 725 1037 1265 1320 1529 1835 1859
Size: 187 537 312 228 55 209 306 24
Cuts at: 1859 2226 2693 2716 3051 3297 4309 4395 4407
Size: 367 467 23 335 246 1012 86 12
Cuts at: 4407 4753 1
Size: 346 449
Fragments arranged by size:
1012 537 467 449 367 346 335 312
306 246 228 209 187 86 55 24
23 12
Bsu36I CC'TnA_GG
Cuts at: 1381 2035 1381
Size: 654 4547
BtgI C'CryG_G
Cuts at: 1686 2009 1686
Size: 323 4878
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1323 3151 3332 3495 1323
Size: 1828 181 163 3029
Fragments arranged by size:
3029 1828 181 163
BtsI GCAGTG_nn'
Cuts at: 266 1738 2444 266
Size: 1472 706 3023
Fragments arranged by size:
3023 1472 706
DraI TTT'AAA
Cuts at: 590 1172 2200 590
Size: 582 1028 3591
Fragments arranged by size:
3591 1028 582
DraIII CAC_nnn'GTG
Cuts at: 1293 2163 1293
Size: 870 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2907 3765 2907
Size: 858 4343
EaeI y'GGCC_r
Cuts at: 816 2786 2960 3351 3378 4723 816
Size: 1970 174 391 27 1345 1294
Fragments arranged by size:
1970 1345 1294 391 174 27
EagI C'GGCC_G
Cuts at: 816 2786 816
Size: 1970 3231
EarI CTCTTCn'nnn_
Cuts at: 935 2121 3224 3434 935
Size: 1186 1103 210 2702
Fragments arranged by size:
2702 1186 1103 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 1989 3409 3719 3865 5011 1921
Size: 68 1420 310 146 1146 2111
Fragments arranged by size:
2111 1420 1146 310 146 68
EcoICRI GAG'CTC
Cuts at: 2213 2213
Size: 5201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 496 595 1080 3026 3458 4205 4473 151
Size: 345 99 485 1946 432 747 268 879
Fragments arranged by size:
1946 879 747 485 432 345 268 99
EcoNI CCTnn'n_nnAGG
Cuts at: 733 733
Size: 5201
EcoO109I rG'GnC_Cy
Cuts at: 216 551 1559 2153 2340 216
Size: 335 1008 594 187 3077
Fragments arranged by size:
3077 1008 594 335 187
EcoRI G'AATT_C
Cuts at: 2205 2205
Size: 5201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 5201
FspI TGC'GCA
Cuts at: 2982 2982
Size: 5201
HaeII r_GCGC'y
Cuts at: 928 2635 2883 3905 4884 928
Size: 1707 248 1022 979 1245
Fragments arranged by size:
1707 1245 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 754 786 936 968 1405 1437 1827 1859 2056
Size: 32 150 32 437 32 390 32 197
Cuts at: 2056 2088 2161 2193 3043 3073 3075 3105 754
Size: 32 73 32 850 30 2 30 2850
Fragments arranged by size:
2850 850 437 390 197 150 73 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1661 1874 2240 1661
Size: 213 366 4622
Fragments arranged by size:
4622 366 213
HpaI GTT'AAC
Cuts at: 1661 1874 1661
Size: 213 4988
KasI G'GCGC_C
Cuts at: 924 2631 2879 924
Size: 1707 248 3246
Fragments arranged by size:
3246 1707 248
KpnI G_GTAC'C
Cuts at: 2221 2221
Size: 5201
MfeI C'AATT_G
Cuts at: 1715 1715
Size: 5201
MluI A'CGCG_T
Cuts at: 4634 4634
Size: 5201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1949 3872 4056 1298
Size: 651 1923 184 2443
Fragments arranged by size:
2443 1923 651 184
MscI TGG'CCA
Cuts at: 2962 2962
Size: 5201
MslI CAynn'nnrTG
Cuts at: 1717 1960 2011 2023 3190 3317 1717
Size: 243 51 12 1167 127 3601
Fragments arranged by size:
3601 1167 243 127 51 12
MspA1I CmG'CkG
Cuts at: 154 426 1145 1848 2312 2626 2986 3999 4244
Size: 272 719 703 464 314 360 1013 245
Cuts at: 4244 154
Size: 1111
Fragments arranged by size:
1111 1013 719 703 464 360 314 272
245
NaeI GCC'GGC
Cuts at: 3382 3382
Size: 5201
NarI GG'CG_CC
Cuts at: 925 2632 2880 925
Size: 1707 248 3246
Fragments arranged by size:
3246 1707 248
NgoMIV G'CCGG_C
Cuts at: 3380 3380
Size: 5201
NspI r_CATG'y
Cuts at: 2022 2254 2754 3661 2022
Size: 232 500 907 3562
Fragments arranged by size:
3562 907 500 232
PasI CC'CwG_GG
Cuts at: 1132 1159 1697 1132
Size: 27 538 4636
Fragments arranged by size:
4636 538 27
PciI A'CATG_T
Cuts at: 3657 3657
Size: 5201
PfoI T'CCnGG_A
Cuts at: 557 954 1096 557
Size: 397 142 4662
Fragments arranged by size:
4662 397 142
PmeI GTTT'AAAC
Cuts at: 2200 2200
Size: 5201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 4340
Size: 32 5169
PpuMI rG'GwC_Cy
Cuts at: 216 1559 216
Size: 1343 3858
PshAI GACnn'nnGTC
Cuts at: 887 887
Size: 5201
PsiI TTA'TAA
Cuts at: 353 353
Size: 5201
PspOMI G'GGCC_C
Cuts at: 2153 2153
Size: 5201
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1456 1488 1456
Size: 32 5169
PstI C_TGCA'G
Cuts at: 260 425 851 1144 2248 2933 260
Size: 165 426 293 1104 685 2528
Fragments arranged by size:
2528 1104 685 426 293 165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5201
PvuII CAG'CTG
Cuts at: 426 1145 1848 2626 2986 426
Size: 719 703 778 360 2641
Fragments arranged by size:
2641 778 719 703 360
RsrII CG'GwC_CG
Cuts at: 3396 3396
Size: 5201
SacI G_AGCT'C
Cuts at: 2215 2215
Size: 5201
SalI G'TCGA_C
Cuts at: 2238 2238
Size: 5201
SapI GCTCTTCn'nnn_
Cuts at: 3224 3434 3224
Size: 210 4991
SbfI CC_TGCA'GG
Cuts at: 2248 2248
Size: 5201
ScaI AGT'ACT
Cuts at: 4718 4718
Size: 5201
SfcI C'TryA_G
Cuts at: 33 256 421 847 1140 1346 2098 2244 2929
Size: 223 165 426 293 206 752 146 685
Cuts at: 2929 3922 4113 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 752 685 426 293 223 206
191 165 146
SfoI GGC'GCC
Cuts at: 926 2633 2881 926
Size: 1707 248 3246
Fragments arranged by size:
3246 1707 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5201
SgrAI Cr'CCGG_yG
Cuts at: 1788 1788
Size: 5201
SmaI CCC'GGG
Cuts at: 548 1426 1499 1973 2223 548
Size: 878 73 474 250 3526
Fragments arranged by size:
3526 878 474 250 73
SmlI C'TyrA_G
Cuts at: 1774 2356 3271 3763 4025 1774
Size: 582 915 492 262 2950
Fragments arranged by size:
2950 915 582 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5201
SphI G_CATG'C
Cuts at: 2022 2254 2754 2022
Size: 232 500 4469
Fragments arranged by size:
4469 500 232
StyI C'CwwG_G
Cuts at: 745 2335 2689 745
Size: 1590 354 3257
Fragments arranged by size:
3257 1590 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 2784 2917 3376 3585 661
Size: 2123 133 459 209 2277
Fragments arranged by size:
2277 2123 459 209 133
TatI w'GTAC_w
Cuts at: 1472 4716 1472
Size: 3244 1957
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1369 1401 1912 1944 4718 4750 1369
Size: 32 511 32 2774 32 1820
Fragments arranged by size:
2774 1820 511 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2998 2998
Size: 5201
XbaI T'CTAG_A
Cuts at: 2232 2232
Size: 5201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1962 2696 1962
Size: 734 4467
XmaI C'CCGG_G
Cuts at: 546 1424 1497 1971 2221 546
Size: 878 73 474 250 3526
Fragments arranged by size:
3526 878 474 250 73
XmnI GAAnn'nnTTC
Cuts at: 398 794 398
Size: 396 4805
ZraI GAC'GTC
Cuts at: 137 137
Size: 5201
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI AscI AvrII BaeI BbsI
BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PspXI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI