(Circular) (Six-base) MAPSORT of: pF1KA1913.seq Check: 6899 from: 1 to: 4586 pF1KA1913 4586 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 1624 1624 Size: 4586 Acc65I G'GTAC_C Cuts at: 1602 1602 Size: 4586 AclI AA'CG_TT Cuts at: 4426 4426 Size: 4586 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1055 2411 2843 3590 3858 1055 Size: 1356 432 747 268 1783 Fragments arranged by size: 1783 1356 747 432 268 AflIII A'CryG_T Cuts at: 1938 3042 4019 1938 Size: 1104 977 2505 Fragments arranged by size: 2505 1104 977 AgeI A'CCGG_T Cuts at: 2974 2974 Size: 4586 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 179 179 Size: 4586 AlwNI CAG_nnn'CTG Cuts at: 1891 3458 1891 Size: 1567 3019 ApaLI G'TGCA_C Cuts at: 3356 3356 Size: 4586 ApoI r'AATT_y Cuts at: 1068 1590 3939 4195 1068 Size: 522 2349 256 1459 Fragments arranged by size: 2349 1459 522 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4586 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4586 AvaI C'yCGr_G Cuts at: 239 714 747 1606 239 Size: 475 33 859 3219 Fragments arranged by size: 3219 859 475 33 AvrII C'CTAG_G Cuts at: 1366 1366 Size: 4586 BamHI G'GATC_C Cuts at: 1611 1611 Size: 4586 BanI G'GyrC_C Cuts at: 230 1602 2016 2264 2299 230 Size: 1372 414 248 35 2517 Fragments arranged by size: 2517 1372 414 248 35 BanII G_rGCy'C Cuts at: 706 1016 1600 2630 706 Size: 310 584 1030 2662 Fragments arranged by size: 2662 1030 584 310 BbeI G_GCGC'C Cuts at: 234 2020 2268 234 Size: 1786 248 2552 Fragments arranged by size: 2552 1786 248 BbsI GAAGACnn'nnnn_ Cuts at: 1251 1424 1251 Size: 173 4413 BbvCI CC'TCA_GC Cuts at: 190 190 Size: 4586 BciVI GTATCCnnnnn_n' Cuts at: 2484 3245 4586 2484 Size: 761 1341 2484 Fragments arranged by size: 2484 1341 761 BclI T'GATC_A Cuts at: 983 2106 983 Size: 1123 3463 BglI GCCn_nnn'nGGC Cuts at: 228 228 Size: 4586 BglII A'GATC_T Cuts at: 1501 2101 4138 1501 Size: 600 2037 1949 Fragments arranged by size: 2037 1949 600 BlpI GC'TnA_GC Cuts at: 1698 1698 Size: 4586 Bme1580I G_kGCm'C Cuts at: 1445 2211 2304 3360 1445 Size: 766 93 1056 2671 Fragments arranged by size: 2671 1056 766 93 BmrI ACTGGGnnnn_n' Cuts at: 1010 1798 1879 2214 4358 1010 Size: 788 81 335 2144 1238 Fragments arranged by size: 2144 1238 788 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 4586 Bpu10I CC'TnA_GC Cuts at: 190 190 Size: 4586 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1762 2641 3133 3431 1762 Size: 879 492 298 2917 Fragments arranged by size: 2917 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 562 4290 562 Size: 3728 858 BsaAI yAC'GTr Cuts at: 1939 1939 Size: 4586 BsaBI GATnn'nnATC Cuts at: 6 2100 4137 6 Size: 2094 2037 455 Fragments arranged by size: 2094 2037 455 BsaHI Gr'CG_yC Cuts at: 231 2017 2265 2967 4463 231 Size: 1786 248 702 1496 354 Fragments arranged by size: 1786 1496 702 354 248 BsaWI w'CCGG_w Cuts at: 1775 2296 2974 3248 3395 3806 1775 Size: 521 678 274 147 411 2555 Fragments arranged by size: 2555 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 693 723 1156 1186 693 Size: 30 433 30 4093 Fragments arranged by size: 4093 433 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 802 802 Size: 4586 BseYI C'CCAG_C Cuts at: 2011 2769 3346 3842 2011 Size: 758 577 496 2755 Fragments arranged by size: 2755 758 577 496 BsiEI CG_ry'CG Cuts at: 104 737 2128 2174 3382 104 Size: 633 1391 46 1208 1308 Fragments arranged by size: 1391 1308 1208 633 46 BsiHKAI G_wGCw'C Cuts at: 317 706 1600 2378 2568 3360 317 Size: 389 894 778 190 792 1543 Fragments arranged by size: 1543 894 792 778 389 190 BsmI GAATG_Cn' Cuts at: 468 468 Size: 4586 BsmBI CGTCTCn'nnnn_ Cuts at: 203 203 Size: 4586 Bsp1286I G_dGCh'C Cuts at: 317 706 1016 1445 1600 2211 2304 2378 2568 Size: 389 310 429 155 611 93 74 190 Cuts at: 2568 2630 3360 317 Size: 62 730 1543 Fragments arranged by size: 1543 730 611 429 389 310 190 155 93 74 62 BspEI T'CCGG_A Cuts at: 3806 3806 Size: 4586 BspHI T'CATG_A Cuts at: 549 3762 549 Size: 3213 1373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1636 1850 2152 2533 1636 Size: 214 302 381 3689 Fragments arranged by size: 3689 381 302 214 BsrBI CCG'CTC Cuts at: 50 2878 2932 4416 4581 50 Size: 2828 54 1484 165 55 Fragments arranged by size: 2828 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 973 2498 973 Size: 1525 3061 BsrFI r'CCGG_y Cuts at: 233 2584 2765 2974 233 Size: 2351 181 209 1845 Fragments arranged by size: 2351 1845 209 181 BsrGI T'GTAC_A Cuts at: 659 659 Size: 4586 BssSI C'ACGA_G Cuts at: 2857 3215 2857 Size: 358 4228 BstAPI GCAn_nnn'nTGC Cuts at: 465 834 1963 465 Size: 369 1129 3088 Fragments arranged by size: 3088 1129 369 BstBI TT'CG_AA Cuts at: 768 2947 768 Size: 2179 2407 BstYI r'GATC_y Cuts at: 1 1501 1611 2078 2101 2436 2682 3694 3780 Size: 1500 110 467 23 335 246 1012 86 Cuts at: 3780 3792 4138 1 Size: 12 346 449 Fragments arranged by size: 1500 1012 467 449 346 335 246 110 86 23 12 BtgI C'CryG_G Cuts at: 503 782 503 Size: 279 4307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2536 2717 2880 2536 Size: 181 163 4242 Fragments arranged by size: 4242 181 163 BtsI GCAGTG_nn' Cuts at: 1829 1829 Size: 4586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 591 626 591 Size: 35 4551 DraI TTT'AAA Cuts at: 1585 1585 Size: 4586 DrdI GACnn_nn'nnGTC Cuts at: 2292 3150 2292 Size: 858 3728 EaeI y'GGCC_r Cuts at: 140 341 2171 2345 2736 2763 4108 140 Size: 201 1830 174 391 27 1345 618 Fragments arranged by size: 1830 1345 618 391 201 174 27 EagI C'GGCC_G Cuts at: 2171 2171 Size: 4586 EarI CTCTTCn'nnn_ Cuts at: 2609 2819 2609 Size: 210 4376 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2794 3104 3250 4396 2794 Size: 310 146 1146 2984 Fragments arranged by size: 2984 1146 310 146 EcoICRI GAG'CTC Cuts at: 704 1598 704 Size: 894 3692 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1055 2411 2843 3590 3858 1055 Size: 1356 432 747 268 1783 Fragments arranged by size: 1783 1356 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 150 150 Size: 4586 EcoO109I rG'GnC_Cy Cuts at: 1315 1725 1315 Size: 410 4176 EcoRI G'AATT_C Cuts at: 1590 1590 Size: 4586 EcoRV GAT'ATC Cuts at: 594 594 Size: 4586 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 4586 FspI TGC'GCA Cuts at: 2367 2367 Size: 4586 HaeII r_GCGC'y Cuts at: 234 2020 2268 3290 4269 234 Size: 1786 248 1022 979 551 Fragments arranged by size: 1786 1022 979 551 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 302 334 1156 1188 2428 2458 2460 2490 302 Size: 32 822 32 1240 30 2 30 2398 Fragments arranged by size: 2398 1240 822 32 32 30 30 2 HincII GTy'rAC Cuts at: 186 401 1625 186 Size: 215 1224 3147 Fragments arranged by size: 3147 1224 215 HindIII A'AGCT_T Cuts at: 1483 1483 Size: 4586 KasI G'GCGC_C Cuts at: 230 2016 2264 230 Size: 1786 248 2552 Fragments arranged by size: 2552 1786 248 KpnI G_GTAC'C Cuts at: 1606 1606 Size: 4586 MfeI C'AATT_G Cuts at: 944 944 Size: 4586 MluI A'CGCG_T Cuts at: 4019 4019 Size: 4586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1352 3257 3441 1352 Size: 1905 184 2497 Fragments arranged by size: 2497 1905 184 MscI TGG'CCA Cuts at: 2347 2347 Size: 4586 MslI CAynn'nnrTG Cuts at: 583 2575 2702 583 Size: 1992 127 2467 Fragments arranged by size: 2467 1992 127 MspA1I CmG'CkG Cuts at: 917 1697 2011 2371 3384 3629 917 Size: 780 314 360 1013 245 1874 Fragments arranged by size: 1874 1013 780 360 314 245 NaeI GCC'GGC Cuts at: 235 2767 235 Size: 2532 2054 NarI GG'CG_CC Cuts at: 231 2017 2265 231 Size: 1786 248 2552 Fragments arranged by size: 2552 1786 248 NcoI C'CATG_G Cuts at: 503 503 Size: 4586 NgoMIV G'CCGG_C Cuts at: 233 2765 233 Size: 2532 2054 NspI r_CATG'y Cuts at: 184 660 1639 2139 3046 184 Size: 476 979 500 907 1724 Fragments arranged by size: 1724 979 907 500 476 PciI A'CATG_T Cuts at: 3042 3042 Size: 4586 PmeI GTTT'AAAC Cuts at: 1585 1585 Size: 4586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 3725 Size: 32 4554 PpuMI rG'GwC_Cy Cuts at: 1315 1315 Size: 4586 PshAI GACnn'nnGTC Cuts at: 1313 1313 Size: 4586 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 643 675 643 Size: 32 4554 PstI C_TGCA'G Cuts at: 1633 2318 1633 Size: 685 3901 PvuI CG_AT'CG Cuts at: 104 737 104 Size: 633 3953 PvuII CAG'CTG Cuts at: 2011 2371 2011 Size: 360 4226 RsrII CG'GwC_CG Cuts at: 2781 2781 Size: 4586 SacI G_AGCT'C Cuts at: 706 1600 706 Size: 894 3692 SalI G'TCGA_C Cuts at: 1623 1623 Size: 4586 SanDI GG'GwC_CC Cuts at: 1315 1315 Size: 4586 SapI GCTCTTCn'nnn_ Cuts at: 2609 2819 2609 Size: 210 4376 SbfI CC_TGCA'GG Cuts at: 1633 1633 Size: 4586 ScaI AGT'ACT Cuts at: 4103 4103 Size: 4586 SfcI C'TryA_G Cuts at: 33 1629 2314 3307 3498 33 Size: 1596 685 993 191 1121 Fragments arranged by size: 1596 1121 993 685 191 SfoI GGC'GCC Cuts at: 232 2018 2266 232 Size: 1786 248 2552 Fragments arranged by size: 2552 1786 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4586 SmaI CCC'GGG Cuts at: 241 1608 241 Size: 1367 3219 SmlI C'TyrA_G Cuts at: 714 1741 2656 3148 3410 714 Size: 1027 915 492 262 1890 Fragments arranged by size: 1890 1027 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4586 SphI G_CATG'C Cuts at: 1639 2139 1639 Size: 500 4086 StyI C'CwwG_G Cuts at: 503 1144 1298 1366 1720 2074 503 Size: 641 154 68 354 354 3015 Fragments arranged by size: 3015 641 354 354 154 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2169 2302 2761 2970 2169 Size: 133 459 209 3785 Fragments arranged by size: 3785 459 209 133 TatI w'GTAC_w Cuts at: 659 1181 4101 659 Size: 522 2920 1144 Fragments arranged by size: 2920 1144 522 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 693 725 1279 1311 4103 4135 693 Size: 32 554 32 2792 32 1144 Fragments arranged by size: 2792 1144 554 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2383 2383 Size: 4586 XbaI T'CTAG_A Cuts at: 1617 1617 Size: 4586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2081 2081 Size: 4586 XhoI C'TCGA_G Cuts at: 714 714 Size: 4586 XmaI C'CCGG_G Cuts at: 239 1606 239 Size: 1367 3219 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI BaeI BcgI BfrBI BmgBI BmtI BpmI BsgI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII FseI FspAI HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PsiI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI