Restriction map for
pF1KA1927
(Circular) (Six-base) MAPSORT of: pF1KA1927.seq Check: 9550 from: 1 to: 6890
pF1KA1927 6890 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2077 2632 3928 2077
Size: 555 1296 5039
Fragments arranged by size:
5039 1296 555
Acc65I G'GTAC_C
Cuts at: 3906 3906
Size: 6890
AclI AA'CG_TT
Cuts at: 6730 6730
Size: 6890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 668 1663 1664 1816 1994 2459 2810 3136
Size: 180 995 1 152 178 465 351 326
Cuts at: 3136 3370 3671 3704 4715 5147 5894 6162 488
Size: 234 301 33 1011 432 747 268 1216
Fragments arranged by size:
1216 1011 995 747 465 432 351 326
301 268 234 180 178 152 33 1
AfeI AGC'GCT
Cuts at: 3273 3273
Size: 6890
AflIII A'CryG_T
Cuts at: 4242 5346 6323 4242
Size: 1104 977 4809
Fragments arranged by size:
4809 1104 977
AgeI A'CCGG_T
Cuts at: 5278 5278
Size: 6890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3224 3224
Size: 6890
AlwNI CAG_nnn'CTG
Cuts at: 206 1213 3159 3322 3418 3601 3727 4195 5762
Size: 1007 1946 163 96 183 126 468 1567
Cuts at: 5762 206
Size: 1334
Fragments arranged by size:
1946 1567 1334 1007 468 183 163 126
96
ApaLI G'TGCA_C
Cuts at: 1451 5660 1451
Size: 4209 2681
ApoI r'AATT_y
Cuts at: 570 653 704 1014 1241 2065 3012 3132 3392
Size: 83 51 310 227 824 947 120 260
Cuts at: 3392 3894 6243 6499 570
Size: 502 2349 256 961
Fragments arranged by size:
2349 961 947 824 502 310 260 256
227 120 83 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 6890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6890
AvaI C'yCGr_G
Cuts at: 3910 3910
Size: 6890
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3476 3509 3476
Size: 33 6857
BamHI G'GATC_C
Cuts at: 3915 3915
Size: 6890
BanI G'GyrC_C
Cuts at: 2556 3906 4320 4568 4603 2556
Size: 1350 414 248 35 4843
Fragments arranged by size:
4843 1350 414 248 35
BanII G_rGCy'C
Cuts at: 2140 2202 2347 2990 3660 3904 4934 2140
Size: 62 145 643 670 244 1030 4096
Fragments arranged by size:
4096 1030 670 643 244 145 62
BbeI G_GCGC'C
Cuts at: 2560 4324 4572 2560
Size: 1764 248 4878
Fragments arranged by size:
4878 1764 248
BbsI GAAGACnn'nnnn_
Cuts at: 1766 1766
Size: 6890
BbvCI CC'TCA_GC
Cuts at: 3728 3728
Size: 6890
BciVI GTATCCnnnnn_n'
Cuts at: 4788 5549 6890 4788
Size: 761 1341 4788
Fragments arranged by size:
4788 1341 761
BclI T'GATC_A
Cuts at: 3111 4410 3111
Size: 1299 5591
BfrBI ATG'CAT
Cuts at: 3738 3738
Size: 6890
BglI GCCn_nnn'nGGC
Cuts at: 3188 3188
Size: 6890
BglII A'GATC_T
Cuts at: 4405 6442 4405
Size: 2037 4853
BlpI GC'TnA_GC
Cuts at: 4002 4002
Size: 6890
Bme1580I G_kGCm'C
Cuts at: 329 1455 4515 4608 5664 329
Size: 1126 3060 93 1056 1555
Fragments arranged by size:
3060 1555 1126 1056 93
BmgBI CAC'GTC
Cuts at: 679 679
Size: 6890
BmrI ACTGGGnnnn_n'
Cuts at: 1333 2162 2341 4102 4183 4518 6662 1333
Size: 829 179 1761 81 335 2144 1561
Fragments arranged by size:
2144 1761 1561 829 335 179 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5257 5257
Size: 6890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 2369 2974 3103 3193 3214 3422 3509 3599
Size: 2197 605 129 90 21 208 87 90
Cuts at: 3599 172
Size: 3463
Fragments arranged by size:
3463 2197 605 208 129 90 90 87
21
Bpu10I CC'TnA_GC
Cuts at: 3728 3728
Size: 6890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 394 866 4066 4945 5437 5735 337
Size: 57 472 3200 879 492 298 1492
Fragments arranged by size:
3200 1492 879 492 472 298 57
BsaI GGTCTCn'nnnn_
Cuts at: 2321 2389 6594 2321
Size: 68 4205 2617
Fragments arranged by size:
4205 2617 68
BsaAI yAC'GTr
Cuts at: 1606 4243 1606
Size: 2637 4253
BsaBI GATnn'nnATC
Cuts at: 6 4404 6441 6
Size: 4398 2037 455
Fragments arranged by size:
4398 2037 455
BsaHI Gr'CG_yC
Cuts at: 895 2557 4321 4569 5271 6767 895
Size: 1662 1764 248 702 1496 1018
Fragments arranged by size:
1764 1662 1496 1018 702 248
BsaWI w'CCGG_w
Cuts at: 4079 4600 5278 5552 5699 6110 4079
Size: 521 678 274 147 411 4859
Fragments arranged by size:
4859 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 2142 2172 3085 3115 217
Size: 30 1895 30 913 30 3992
Fragments arranged by size:
3992 1895 913 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 274 277 286 289 1535 1825 1828 2458
Size: 117 3 9 3 1246 290 3 630
Cuts at: 2458 3106 3109 3190 3211 3228 157
Size: 648 3 81 21 17 3819
Fragments arranged by size:
3819 1246 648 630 290 117 81 21
17 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3186 4315 5073 5650 6146 3186
Size: 1129 758 577 496 3930
Fragments arranged by size:
3930 1129 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 3625
Size: 6890
BsiEI CG_ry'CG
Cuts at: 104 4432 4478 5686 104
Size: 4328 46 1208 1308
Fragments arranged by size:
4328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 774 1455 2140 2202 2690 2707 2812 2990 3904
Size: 681 685 62 488 17 105 178 914
Cuts at: 3904 4682 4872 5664 774
Size: 778 190 792 2000
Fragments arranged by size:
2000 914 792 778 685 681 488 190
178 105 62 17
BsmI GAATG_Cn'
Cuts at: 1230 1620 2578 1230
Size: 390 958 5542
Fragments arranged by size:
5542 958 390
BsmBI CGTCTCn'nnnn_
Cuts at: 214 214
Size: 6890
Bsp1286I G_dGCh'C
Cuts at: 329 774 1455 2140 2202 2347 2690 2707 2812
Size: 445 681 685 62 145 343 17 105
Cuts at: 2812 2990 3660 3904 4515 4608 4682 4872 4934
Size: 178 670 244 611 93 74 190 62
Cuts at: 4934 5664 329
Size: 730 1555
Fragments arranged by size:
1555 730 685 681 670 611 445 343
244 190 178 145 105 93 74 62
62 17
BspEI T'CCGG_A
Cuts at: 6110 6110
Size: 6890
BspHI T'CATG_A
Cuts at: 1311 6066 1311
Size: 4755 2135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3940 4154 4456 4837 3940
Size: 214 302 381 5993
Fragments arranged by size:
5993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3236 5182 5236 6720 6885 50
Size: 3186 1946 54 1484 165 55
Fragments arranged by size:
3186 1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3056 4802 3056
Size: 1746 5144
BsrFI r'CCGG_y
Cuts at: 113 300 4888 5069 5278 113
Size: 187 4588 181 209 1725
Fragments arranged by size:
4588 1725 209 187 181
BssHII G'CGCG_C
Cuts at: 290 290
Size: 6890
BssSI C'ACGA_G
Cuts at: 5161 5519 5161
Size: 358 6532
BstAPI GCAn_nnn'nTGC
Cuts at: 1861 4267 1861
Size: 2406 4484
BstBI TT'CG_AA
Cuts at: 1508 5251 1508
Size: 3743 3147
BstYI r'GATC_y
Cuts at: 1 228 580 642 3915 4382 4405 4740 4986
Size: 227 352 62 3273 467 23 335 246
Cuts at: 4986 5998 6084 6096 6442 1
Size: 1012 86 12 346 449
Fragments arranged by size:
3273 1012 467 449 352 346 335 246
227 86 62 23 12
BstZ17I GTA'TAC
Cuts at: 2078 2078
Size: 6890
BtgI C'CryG_G
Cuts at: 107 2168 2560 3068 107
Size: 2061 392 508 3929
Fragments arranged by size:
3929 2061 508 392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4840 5021 5184 4840
Size: 181 163 6546
Fragments arranged by size:
6546 181 163
BtsI GCAGTG_nn'
Cuts at: 977 1410 1638 2061 2133 2875 4133 977
Size: 433 228 423 72 742 1258 3734
Fragments arranged by size:
3734 1258 742 433 423 228 72
DraI TTT'AAA
Cuts at: 3889 3889
Size: 6890
DraIII CAC_nnn'GTG
Cuts at: 1756 1756
Size: 6890
DrdI GACnn_nn'nnGTC
Cuts at: 4596 5454 4596
Size: 858 6032
EaeI y'GGCC_r
Cuts at: 524 854 4475 4649 5040 5067 6412 524
Size: 330 3621 174 391 27 1345 1002
Fragments arranged by size:
3621 1345 1002 391 330 174 27
EagI C'GGCC_G
Cuts at: 4475 4475
Size: 6890
EarI CTCTTCn'nnn_
Cuts at: 309 1511 1835 1976 2169 2435 2844 4913 5123
Size: 1202 324 141 193 266 409 2069 210
Cuts at: 5123 309
Size: 2076
Fragments arranged by size:
2076 2069 1202 409 324 266 210 193
141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 154 3018 5098 5408 5554 6700 148
Size: 6 2864 2080 310 146 1146 338
Fragments arranged by size:
2864 2080 1146 338 310 146 6
EcoICRI GAG'CTC
Cuts at: 2138 2200 2988 3902 2138
Size: 62 788 914 5126
Fragments arranged by size:
5126 914 788 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 488 668 1663 1664 1816 1994 2369 2459
Size: 316 180 995 1 152 178 375 90
Cuts at: 2459 2810 2974 3103 3136 3193 3214 3370 3422
Size: 351 164 129 33 57 21 156 52
Cuts at: 3422 3509 3599 3671 3704 4715 5147 5894 6162
Size: 87 90 72 33 1011 432 747 268
Cuts at: 6162 172
Size: 900
Fragments arranged by size:
1011 995 900 747 432 375 351 316
268 180 178 164 156 152 129 90
90 87 72 57 52 33 33 21
1
EcoO109I rG'GnC_Cy
Cuts at: 279 2675 2911 3156 3673 4029 279
Size: 2396 236 245 517 356 3140
Fragments arranged by size:
3140 2396 517 356 245 236
EcoRI G'AATT_C
Cuts at: 570 3894 570
Size: 3324 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 673 1521 4909 673
Size: 848 3388 2654
Fragments arranged by size:
3388 2654 848
FspI TGC'GCA
Cuts at: 3217 4671 3217
Size: 1454 5436
FspAI rTGC'GCAy
Cuts at: 3217 3217
Size: 6890
HaeII r_GCGC'y
Cuts at: 2560 3183 3275 4324 4572 5594 6573 2560
Size: 623 92 1049 248 1022 979 2877
Fragments arranged by size:
2877 1049 1022 979 623 248 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 217 245 249 439 471 1117 1149 1648
Size: 4 28 4 190 32 646 32 499
Cuts at: 1648 1680 1801 1833 2122 2154 2470 2502 3268
Size: 32 121 32 289 32 316 32 766
Cuts at: 3268 3300 4732 4762 4764 4794 213
Size: 32 1432 30 2 30 2309
Fragments arranged by size:
2309 1432 766 646 499 316 289 190
121 32 32 32 32 32 32 32
30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 3929 3929
Size: 6890
KasI G'GCGC_C
Cuts at: 2556 4320 4568 2556
Size: 1764 248 4878
Fragments arranged by size:
4878 1764 248
KpnI G_GTAC'C
Cuts at: 3910 3910
Size: 6890
MluI A'CGCG_T
Cuts at: 6323 6323
Size: 6890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 605 5561 5745 197
Size: 408 4956 184 1342
Fragments arranged by size:
4956 1342 408 184
MscI TGG'CCA
Cuts at: 526 856 4651 526
Size: 330 3795 2765
Fragments arranged by size:
3795 2765 330
MslI CAynn'nnrTG
Cuts at: 482 488 2783 2836 3253 3743 4879 5006 482
Size: 6 2295 53 417 490 1136 127 2366
Fragments arranged by size:
2366 2295 1136 490 417 127 53 6
MspA1I CmG'CkG
Cuts at: 297 307 1069 1213 2562 3171 3649 3724 4001
Size: 10 762 144 1349 609 478 75 277
Cuts at: 4001 4315 4675 5688 5933 297
Size: 314 360 1013 245 1254
Fragments arranged by size:
1349 1254 1013 762 609 478 360 314
277 245 144 75 10
NaeI GCC'GGC
Cuts at: 302 5071 302
Size: 4769 2121
NarI GG'CG_CC
Cuts at: 2557 4321 4569 2557
Size: 1764 248 4878
Fragments arranged by size:
4878 1764 248
NcoI C'CATG_G
Cuts at: 107 2168 3068 107
Size: 2061 900 3929
Fragments arranged by size:
3929 2061 900
NdeI CA'TA_TG
Cuts at: 3083 3083
Size: 6890
NgoMIV G'CCGG_C
Cuts at: 300 5069 300
Size: 4769 2121
NruI TCG'CGA
Cuts at: 343 343
Size: 6890
NsiI A_TGCA'T
Cuts at: 3740 3740
Size: 6890
NspI r_CATG'y
Cuts at: 2788 3222 3943 4443 5350 2788
Size: 434 721 500 907 4328
Fragments arranged by size:
4328 907 721 500 434
PciI A'CATG_T
Cuts at: 5346 5346
Size: 6890
PfoI T'CCnGG_A
Cuts at: 889 2385 3399 889
Size: 1496 1014 4380
Fragments arranged by size:
4380 1496 1014
PmeI GTTT'AAAC
Cuts at: 3889 3889
Size: 6890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6029 6061 6029
Size: 32 6858
PpuMI rG'GwC_Cy
Cuts at: 279 2911 3156 3673 279
Size: 2632 245 517 3496
Fragments arranged by size:
3496 2632 517 245
PsiI TTA'TAA
Cuts at: 662 662
Size: 6890
PstI C_TGCA'G
Cuts at: 905 2882 3170 3937 4622 905
Size: 1977 288 767 685 3173
Fragments arranged by size:
3173 1977 767 685 288
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6890
PvuII CAG'CTG
Cuts at: 297 1069 1213 3171 3649 3724 4315 4675 297
Size: 772 144 1958 478 75 591 360 2512
Fragments arranged by size:
2512 1958 772 591 478 360 144 75
RsrII CG'GwC_CG
Cuts at: 5085 5085
Size: 6890
SacI G_AGCT'C
Cuts at: 2140 2202 2990 3904 2140
Size: 62 788 914 5126
Fragments arranged by size:
5126 914 788 62
SacII CC_GC'GG
Cuts at: 2563 2563
Size: 6890
SalI G'TCGA_C
Cuts at: 3927 3927
Size: 6890
SapI GCTCTTCn'nnn_
Cuts at: 4913 5123 4913
Size: 210 6680
SbfI CC_TGCA'GG
Cuts at: 3937 3937
Size: 6890
ScaI AGT'ACT
Cuts at: 2940 6407 2940
Size: 3467 3423
SfcI C'TryA_G
Cuts at: 33 901 1178 1652 1941 2878 3166 3933 4618
Size: 868 277 474 289 937 288 767 685
Cuts at: 4618 5611 5802 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 937 868 767 685 474 289
288 277 191
SfoI GGC'GCC
Cuts at: 2558 4322 4570 2558
Size: 1764 248 4878
Fragments arranged by size:
4878 1764 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6890
SmaI CCC'GGG
Cuts at: 3912 3912
Size: 6890
SmlI C'TyrA_G
Cuts at: 352 409 881 4045 4960 5452 5714 352
Size: 57 472 3164 915 492 262 1528
Fragments arranged by size:
3164 1528 915 492 472 262 57
SnaBI TAC'GTA
Cuts at: 1606 1606
Size: 6890
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6890
SphI G_CATG'C
Cuts at: 3222 3943 4443 3222
Size: 721 500 5669
Fragments arranged by size:
5669 721 500
StuI AGG'CCT
Cuts at: 184 184
Size: 6890
StyI C'CwwG_G
Cuts at: 107 1272 2168 3068 4024 4378 107
Size: 1165 896 900 956 354 2619
Fragments arranged by size:
2619 1165 956 900 896 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1315 4473 4606 5065 5274 1315
Size: 3158 133 459 209 2931
Fragments arranged by size:
3158 2931 459 209 133
TatI w'GTAC_w
Cuts at: 403 2429 2938 6405 403
Size: 2026 509 3467 888
Fragments arranged by size:
3467 2026 888 509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 579 611 1929 1961 6407 6439 139
Size: 32 408 32 1318 32 4446 32 590
Fragments arranged by size:
4446 1318 590 408 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4687 4687
Size: 6890
XbaI T'CTAG_A
Cuts at: 3589 3921 3589
Size: 332 6558
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4385 4385
Size: 6890
XmaI C'CCGG_G
Cuts at: 3910 3910
Size: 6890
XmnI GAAnn'nnTTC
Cuts at: 653 1520 3550 653
Size: 867 2030 3993
Fragments arranged by size:
3993 2030 867
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NsiI NspI PciI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BcgI BmtI BsiWI BsrGI BstEII BstXI Bsu36I
ClaI CspCI EcoNI EcoRV FseI HindIII HpaI MfeI
NheI NotI PacI PasI PflMI PmlI PshAI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI ZraI