Restriction map for
pF1KA1936
(Circular) (Six-base) MAPSORT of: pF1KA1936.seq Check: 3366 from: 1 to: 5525
pF1KA1936 5525 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1668 1668
Size: 5525
AatII G_ACGT'C
Cuts at: 172 172
Size: 5525
AccI GT'mk_AC
Cuts at: 2563 2563
Size: 5525
Acc65I G'GTAC_C
Cuts at: 2541 2541
Size: 5525
AclI AA'CG_TT
Cuts at: 5365 5365
Size: 5525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 3350 3782 4135 4797 1588
Size: 1762 432 353 662 2316
Fragments arranged by size:
2316 1762 662 432 353
AfeI AGC'GCT
Cuts at: 767 767
Size: 5525
AflIII A'CryG_T
Cuts at: 419 478 1592 2877 4677 4958 419
Size: 59 1114 1285 1800 281 986
Fragments arranged by size:
1800 1285 1114 986 281 59
AhdI GACnn_n'nnGTC
Cuts at: 1109 1109
Size: 5525
AleI CACnn'nnGTG
Cuts at: 1747 1747
Size: 5525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 911 1696 1980 911
Size: 785 284 4456
Fragments arranged by size:
4456 785 284
AlwNI CAG_nnn'CTG
Cuts at: 367 666 1872 2145 2830 4268 367
Size: 299 1206 273 685 1438 1624
Fragments arranged by size:
1624 1438 1206 685 299 273
ApaI G_GGCC'C
Cuts at: 1946 1946
Size: 5525
ApaLI G'TGCA_C
Cuts at: 4363 4363
Size: 5525
ApoI r'AATT_y
Cuts at: 340 1426 2529 4878 5134 340
Size: 1086 1103 2349 256 731
Fragments arranged by size:
2349 1103 1086 731 256
AseI AT'TA_AT
Cuts at: 20 1349 20
Size: 1329 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5525
AvaI C'yCGr_G
Cuts at: 2225 2545 2225
Size: 320 5205
AvrII C'CTAG_G
Cuts at: 1290 1290
Size: 5525
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 499 532 499
Size: 33 5492
BamHI G'GATC_C
Cuts at: 2550 2550
Size: 5525
BanI G'GyrC_C
Cuts at: 2153 2541 2955 3203 3238 2153
Size: 388 414 248 35 4440
Fragments arranged by size:
4440 414 388 248 35
BanII G_rGCy'C
Cuts at: 881 1094 1946 2339 2462 2539 3569 881
Size: 213 852 393 123 77 1030 2837
Fragments arranged by size:
2837 1030 852 393 213 123 77
BbeI G_GCGC'C
Cuts at: 2959 3207 2959
Size: 248 5277
BbsI GAAGACnn'nnnn_
Cuts at: 1374 1374
Size: 5525
BbvCI CC'TCA_GC
Cuts at: 769 1873 2408 769
Size: 1104 535 3886
Fragments arranged by size:
3886 1104 535
BciVI GTATCCnnnnn_n'
Cuts at: 527 3423 4479 5525 527
Size: 2896 1056 1046 527
Fragments arranged by size:
2896 1056 1046 527
BclI T'GATC_A
Cuts at: 3045 3045
Size: 5525
BglII A'GATC_T
Cuts at: 2020 2354 3040 5077 2020
Size: 334 686 2037 2468
Fragments arranged by size:
2468 2037 686 334
BlpI GC'TnA_GC
Cuts at: 2637 2637
Size: 5525
Bme1580I G_kGCm'C
Cuts at: 1946 3150 3243 4367 1946
Size: 1204 93 1124 3104
Fragments arranged by size:
3104 1204 1124 93
BmgBI CAC'GTC
Cuts at: 479 1560 2001 479
Size: 1081 441 4003
Fragments arranged by size:
4003 1081 441
BmrI ACTGGGnnnn_n'
Cuts at: 1147 2737 2818 3153 5297 1147
Size: 1590 81 335 2144 1375
Fragments arranged by size:
2144 1590 1375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1187 3892 1187
Size: 2705 2820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 2446
Size: 5525
Bpu10I CC'TnA_GC
Cuts at: 769 1616 1873 2389 2408 769
Size: 847 257 516 19 3886
Fragments arranged by size:
3886 847 516 257 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 775 2701 3580 4294 4592 225
Size: 550 1926 879 714 298 1158
Fragments arranged by size:
1926 1158 879 714 550 298
BsaI GGTCTCn'nnnn_
Cuts at: 193 1318 1883 2064 5229 193
Size: 1125 565 181 3165 489
Fragments arranged by size:
3165 1125 565 489 181
BsaAI yAC'GTr
Cuts at: 550 2878 550
Size: 2328 3197
BsaBI GATnn'nnATC
Cuts at: 6 3039 5076 6
Size: 3033 2037 455
Fragments arranged by size:
3033 2037 455
BsaHI Gr'CG_yC
Cuts at: 169 1783 2165 2956 3204 3906 5402 169
Size: 1614 382 791 248 702 1496 292
Fragments arranged by size:
1614 1496 791 702 382 292 248
BsaWI w'CCGG_w
Cuts at: 2714 3235 3913 4324 4471 4745 2714
Size: 521 678 411 147 274 3494
Fragments arranged by size:
3494 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 356 386 1985 2015 2404 2434 356
Size: 30 1599 30 389 30 3447
Fragments arranged by size:
3447 1599 389 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 216 299 790 1506 2292 2425 216
Size: 83 491 716 786 133 3316
Fragments arranged by size:
3316 786 716 491 133 83
BseYI C'CCAG_C
Cuts at: 732 2950 3708 4373 4781 732
Size: 2218 758 665 408 1476
Fragments arranged by size:
2218 1476 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1436 1479 2048 2410 907
Size: 529 43 569 362 4022
Fragments arranged by size:
4022 569 529 362 43
BsiEI CG_ry'CG
Cuts at: 104 3067 3113 4343 104
Size: 2963 46 1230 1286
Fragments arranged by size:
2963 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 881 1094 1424 1440 2192 2339 2395 2462 2539
Size: 213 330 16 752 147 56 67 77
Cuts at: 2539 3317 3507 4367 881
Size: 778 190 860 2039
Fragments arranged by size:
2039 860 778 752 330 213 190 147
77 67 56 16
BsmI GAATG_Cn'
Cuts at: 1957 1957
Size: 5525
BsmBI CGTCTCn'nnnn_
Cuts at: 469 469
Size: 5525
Bsp1286I G_dGCh'C
Cuts at: 881 1094 1424 1440 1946 2192 2339 2395 2462
Size: 213 330 16 506 246 147 56 67
Cuts at: 2462 2539 3150 3243 3317 3507 3569 4367 881
Size: 77 611 93 74 190 62 798 2039
Fragments arranged by size:
2039 798 611 506 330 246 213 190
147 93 77 74 67 62 56 16
BspHI T'CATG_A
Cuts at: 3957 3957
Size: 5525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1668 2076 2575 2789 3091 3472 1668
Size: 408 499 214 302 381 3721
Fragments arranged by size:
3721 499 408 381 302 214
BsrBI CCG'CTC
Cuts at: 50 443 3817 3871 5355 5520 50
Size: 393 3374 54 1484 165 55
Fragments arranged by size:
3374 1484 393 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1154 3437 1154
Size: 2283 3242
BsrFI r'CCGG_y
Cuts at: 3523 3704 3523
Size: 181 5344
BssSI C'ACGA_G
Cuts at: 3796 4504 3796
Size: 708 4817
BstAPI GCAn_nnn'nTGC
Cuts at: 1973 2902 1973
Size: 929 4596
BstBI TT'CG_AA
Cuts at: 3886 3886
Size: 5525
BstXI CCAn_nnnn'nTGG
Cuts at: 1825 1825
Size: 5525
BstYI r'GATC_y
Cuts at: 1 111 825 2020 2354 2550 3017 3040 3375
Size: 110 714 1195 334 196 467 23 335
Cuts at: 3375 3621 3927 3939 4025 5077 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1195 1052 714 467 449 335 334 306
246 196 110 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 245 1162 2241 245
Size: 917 1079 3529
Fragments arranged by size:
3529 1079 917
BtgI C'CryG_G
Cuts at: 107 2195 2304 2437 107
Size: 2088 109 133 3195
Fragments arranged by size:
3195 2088 133 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1594 1636 3475 3656 3819 1594
Size: 42 1839 181 163 3300
Fragments arranged by size:
3300 1839 181 163 42
BtsI GCAGTG_nn'
Cuts at: 377 1541 1818 2768 377
Size: 1164 277 950 3134
Fragments arranged by size:
3134 1164 950 277
DraI TTT'AAA
Cuts at: 833 2524 833
Size: 1691 3834
DraIII CAC_nnn'GTG
Cuts at: 1141 2196 1141
Size: 1055 4470
DrdI GACnn_nn'nnGTC
Cuts at: 970 2056 3231 4575 970
Size: 1086 1175 1344 1920
Fragments arranged by size:
1920 1344 1175 1086
EaeI y'GGCC_r
Cuts at: 743 1019 3110 3284 3675 3702 5047 743
Size: 276 2091 174 391 27 1345 1221
Fragments arranged by size:
2091 1345 1221 391 276 174 27
EagI C'GGCC_G
Cuts at: 3110 3110
Size: 5525
EarI CTCTTCn'nnn_
Cuts at: 234 460 2344 3548 3758 234
Size: 226 1884 1204 210 2001
Fragments arranged by size:
2001 1884 1204 226 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2025 3733 4475 4621 5335 2025
Size: 1708 742 146 714 2215
Fragments arranged by size:
2215 1708 742 714 146
EcoICRI GAG'CTC
Cuts at: 879 1092 2337 2460 2537 879
Size: 213 1245 123 77 3867
Fragments arranged by size:
3867 1245 213 123 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 2446 3350 3782 4135 4797 1588
Size: 858 904 432 353 662 2316
Fragments arranged by size:
2316 904 858 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 833 886 1118 833
Size: 53 232 5240
Fragments arranged by size:
5240 232 53
EcoO109I rG'GnC_Cy
Cuts at: 984 1645 1942 2073 2509 2664 984
Size: 661 297 131 436 155 3845
Fragments arranged by size:
3845 661 436 297 155 131
EcoRI G'AATT_C
Cuts at: 2529 2529
Size: 5525
EcoRV GAT'ATC
Cuts at: 213 213
Size: 5525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3544 3544
Size: 5525
FspI TGC'GCA
Cuts at: 3306 3306
Size: 5525
HaeII r_GCGC'y
Cuts at: 769 1984 2959 3207 4437 5208 769
Size: 1215 975 248 1230 771 1086
Fragments arranged by size:
1230 1215 1086 975 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 406 438 1095 1127 2404 2436 3367
Size: 32 256 32 657 32 1277 32 931
Cuts at: 3367 3397 3399 3429 118
Size: 30 2 30 2214
Fragments arranged by size:
2214 1277 931 657 256 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1169 2564 1169
Size: 1395 4130
HindIII A'AGCT_T
Cuts at: 726 1213 2104 726
Size: 487 891 4147
Fragments arranged by size:
4147 891 487
KasI G'GCGC_C
Cuts at: 2955 3203 2955
Size: 248 5277
KpnI G_GTAC'C
Cuts at: 2545 2545
Size: 5525
MluI A'CGCG_T
Cuts at: 4958 4958
Size: 5525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2465 4284 4468 2465
Size: 1819 184 3522
Fragments arranged by size:
3522 1819 184
MscI TGG'CCA
Cuts at: 745 1021 3286 745
Size: 276 2265 2984
Fragments arranged by size:
2984 2265 276
MslI CAynn'nnrTG
Cuts at: 1747 1823 3514 3641 1747
Size: 76 1691 127 3631
Fragments arranged by size:
3631 1691 127 76
MspA1I CmG'CkG
Cuts at: 1791 2142 2636 2950 3310 4094 4339 1791
Size: 351 494 314 360 784 245 2977
Fragments arranged by size:
2977 784 494 360 351 314 245
NaeI GCC'GGC
Cuts at: 3706 3706
Size: 5525
NarI GG'CG_CC
Cuts at: 2956 3204 2956
Size: 248 5277
NcoI C'CATG_G
Cuts at: 107 2437 107
Size: 2330 3195
NgoMIV G'CCGG_C
Cuts at: 3704 3704
Size: 5525
NspI r_CATG'y
Cuts at: 423 1596 2578 3078 4681 423
Size: 1173 982 500 1603 1267
Fragments arranged by size:
1603 1267 1173 982 500
PasI CC'CwG_GG
Cuts at: 573 573
Size: 5525
PciI A'CATG_T
Cuts at: 419 1592 4677 419
Size: 1173 3085 1267
Fragments arranged by size:
3085 1267 1173
PflMI CCAn_nnn'nTGG
Cuts at: 1336 1336
Size: 5525
PfoI T'CCnGG_A
Cuts at: 1335 2453 1335
Size: 1118 4407
PmeI GTTT'AAAC
Cuts at: 2524 2524
Size: 5525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 5493
PpuMI rG'GwC_Cy
Cuts at: 984 1645 2073 984
Size: 661 428 4436
Fragments arranged by size:
4436 661 428
PsiI TTA'TAA
Cuts at: 1373 1373
Size: 5525
PspOMI G'GGCC_C
Cuts at: 1942 1942
Size: 5525
PstI C_TGCA'G
Cuts at: 372 587 671 1941 2572 3257 372
Size: 215 84 1270 631 685 2640
Fragments arranged by size:
2640 1270 685 631 215 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5525
PvuII CAG'CTG
Cuts at: 2950 3310 2950
Size: 360 5165
RsrII CG'GwC_CG
Cuts at: 3720 3720
Size: 5525
SacI G_AGCT'C
Cuts at: 881 1094 2339 2462 2539 881
Size: 213 1245 123 77 3867
Fragments arranged by size:
3867 1245 213 123 77
SalI G'TCGA_C
Cuts at: 2562 2562
Size: 5525
SapI GCTCTTCn'nnn_
Cuts at: 2344 3548 3758 2344
Size: 1204 210 4111
Fragments arranged by size:
4111 1204 210
SbfI CC_TGCA'GG
Cuts at: 2572 2572
Size: 5525
ScaI AGT'ACT
Cuts at: 5042 5042
Size: 5525
SexAI A'CCwGG_T
Cuts at: 2054 2054
Size: 5525
SfcI C'TryA_G
Cuts at: 33 191 318 368 583 667 1731 1937 2077
Size: 158 127 50 215 84 1064 206 140
Cuts at: 2077 2272 2504 2568 3253 4221 4412 33
Size: 195 232 64 685 968 191 1146
Fragments arranged by size:
1146 1064 968 685 232 215 206 195
191 158 140 127 84 64 50
SfoI GGC'GCC
Cuts at: 2957 3205 2957
Size: 248 5277
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5525
SmaI CCC'GGG
Cuts at: 2227 2547 2227
Size: 320 5205
SmlI C'TyrA_G
Cuts at: 204 754 2680 3595 4309 4571 204
Size: 550 1926 915 714 262 1158
Fragments arranged by size:
1926 1158 915 714 550 262
SnaBI TAC'GTA
Cuts at: 550 550
Size: 5525
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5525
SphI G_CATG'C
Cuts at: 2578 3078 2578
Size: 500 5025
SrfI GCCC'GGGC
Cuts at: 2227 2227
Size: 5525
StuI AGG'CCT
Cuts at: 405 1084 405
Size: 679 4846
StyI C'CwwG_G
Cuts at: 107 1290 2437 2659 3013 107
Size: 1183 1147 222 354 2619
Fragments arranged by size:
2619 1183 1147 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1129 2281 2511 3108 3241 3700 3909 1129
Size: 1152 230 597 133 459 209 2745
Fragments arranged by size:
2745 1152 597 459 230 209 133
TatI w'GTAC_w
Cuts at: 377 5040 377
Size: 4663 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 356 388 1985 2017 2296 2328 5042 5074 356
Size: 32 1597 32 279 32 2714 32 807
Fragments arranged by size:
2714 1597 807 279 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3322 3322
Size: 5525
XbaI T'CTAG_A
Cuts at: 2556 2556
Size: 5525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2322 3020 2322
Size: 698 4827
XmaI C'CCGG_G
Cuts at: 2225 2545 2225
Size: 320 5205
ZraI GAC'GTC
Cuts at: 170 170
Size: 5525
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI BcgI BfrBI BglI BmtI
BsiWI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PspXI PsrI SacII SanDI
SfiI SgrAI SspI SwaI XhoI XmnI