Restriction map for
pF1KA1944
(Circular) (Six-base) MAPSORT of: pF1KA1944.seq Check: 7165 from: 1 to: 6410
pF1KA1944 6410 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1904 1941 1904
Size: 37 6373
AatII G_ACGT'C
Cuts at: 281 281
Size: 6410
AccI GT'mk_AC
Cuts at: 521 3448 521
Size: 2927 3483
Acc65I G'GTAC_C
Cuts at: 3426 3426
Size: 6410
AclI AA'CG_TT
Cuts at: 6250 6250
Size: 6410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1076 4235 4667 5414 5682 313
Size: 763 3159 432 747 268 1041
Fragments arranged by size:
3159 1041 763 747 432 268
AflIII A'CryG_T
Cuts at: 3762 4866 5843 3762
Size: 1104 977 4329
Fragments arranged by size:
4329 1104 977
AgeI A'CCGG_T
Cuts at: 4798 4798
Size: 6410
AhdI GACnn_n'nnGTC
Cuts at: 194 194
Size: 6410
AleI CACnn'nnGTG
Cuts at: 2188 2188
Size: 6410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3089 3385 3089
Size: 296 6114
AlwNI CAG_nnn'CTG
Cuts at: 549 572 1638 1995 2034 2240 3345 3715 5282
Size: 23 1066 357 39 206 1105 370 1567
Cuts at: 5282 549
Size: 1677
Fragments arranged by size:
1677 1567 1105 1066 370 357 206 39
23
ApaI G_GGCC'C
Cuts at: 893 893
Size: 6410
ApaLI G'TGCA_C
Cuts at: 796 5180 796
Size: 4384 2026
ApoI r'AATT_y
Cuts at: 472 1047 2356 2494 3244 3414 5763 6019 472
Size: 575 1309 138 750 170 2349 256 863
Fragments arranged by size:
2349 1309 863 750 575 256 170 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 6410
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6410
AvaI C'yCGr_G
Cuts at: 338 635 2110 3430 338
Size: 297 1475 1320 3318
Fragments arranged by size:
3318 1475 1320 297
BamHI G'GATC_C
Cuts at: 203 854 3435 203
Size: 651 2581 3178
Fragments arranged by size:
3178 2581 651
BanI G'GyrC_C
Cuts at: 137 423 1231 1668 2398 2668 3426 3840 4088
Size: 286 808 437 730 270 758 414 248
Cuts at: 4088 4123 137
Size: 35 2424
Fragments arranged by size:
2424 808 758 730 437 414 286 270
248 35
BanII G_rGCy'C
Cuts at: 701 785 893 1372 1712 1906 1964 3209 3424
Size: 84 108 479 340 194 58 1245 215
Cuts at: 3424 4454 701
Size: 1030 2657
Fragments arranged by size:
2657 1245 1030 479 340 215 194 108
84 58
BbeI G_GCGC'C
Cuts at: 1235 3844 4092 1235
Size: 2609 248 3553
Fragments arranged by size:
3553 2609 248
BbsI GAAGACnn'nnnn_
Cuts at: 839 1450 1544 2877 2898 839
Size: 611 94 1333 21 4351
Fragments arranged by size:
4351 1333 611 94 21
BbvCI CC'TCA_GC
Cuts at: 1862 3118 1862
Size: 1256 5154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3078 3112 3078
Size: 34 6376
BciVI GTATCCnnnnn_n'
Cuts at: 4308 5069 6410 4308
Size: 761 1341 4308
Fragments arranged by size:
4308 1341 761
BclI T'GATC_A
Cuts at: 3930 3930
Size: 6410
BglII A'GATC_T
Cuts at: 1358 3925 5962 1358
Size: 2567 2037 1806
Fragments arranged by size:
2567 2037 1806
BlpI GC'TnA_GC
Cuts at: 2152 2845 3522 2152
Size: 693 677 5040
Fragments arranged by size:
5040 693 677
Bme1580I G_kGCm'C
Cuts at: 115 428 800 893 1673 1739 2671 4035 4128
Size: 313 372 93 780 66 932 1364 93
Cuts at: 4128 5184 115
Size: 1056 1341
Fragments arranged by size:
1364 1341 1056 932 780 372 313 93
93 66
BmgBI CAC'GTC
Cuts at: 1017 1337 1542 1017
Size: 320 205 5885
Fragments arranged by size:
5885 320 205
BmrI ACTGGGnnnn_n'
Cuts at: 582 3006 3622 3703 4038 6182 582
Size: 2424 616 81 335 2144 810
Fragments arranged by size:
2424 2144 810 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 4777 3005
Size: 1772 4638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 715 1026 1528 1672 1731 309
Size: 406 311 502 144 59 4988
Fragments arranged by size:
4988 502 406 311 144 59
Bpu10I CC'TnA_GC
Cuts at: 526 1639 1862 2195 3118 526
Size: 1113 223 333 923 3818
Fragments arranged by size:
3818 1113 923 333 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 3586 4465 4957 5255 229
Size: 3357 879 492 298 1384
Fragments arranged by size:
3357 1384 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 625 1476 1701 6114 625
Size: 851 225 4413 921
Fragments arranged by size:
4413 921 851 225
BsaAI yAC'GTr
Cuts at: 1307 3763 1307
Size: 2456 3954
BsaBI GATnn'nnATC
Cuts at: 6 3924 5961 6
Size: 3918 2037 455
Fragments arranged by size:
3918 2037 455
BsaHI Gr'CG_yC
Cuts at: 278 1232 3841 4089 4791 6287 278
Size: 954 2609 248 702 1496 401
Fragments arranged by size:
2609 1496 954 702 401 248
BsaWI w'CCGG_w
Cuts at: 857 3014 3599 4120 4798 5072 5219 5630 857
Size: 2157 585 521 678 274 147 411 1637
Fragments arranged by size:
2157 1637 678 585 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 688 718 3034 3064 688
Size: 30 2316 30 4034
Fragments arranged by size:
4034 2316 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 756 855 1792 1795 1972 2030 2980 3042 3106
Size: 99 937 3 177 58 950 62 64
Cuts at: 3106 3258 756
Size: 152 3908
Fragments arranged by size:
3908 950 937 177 152 99 64 62
58 3
BseYI C'CCAG_C
Cuts at: 1899 2240 2752 2772 3835 4593 5170 5666 1899
Size: 341 512 20 1063 758 577 496 2643
Fragments arranged by size:
2643 1063 758 577 512 496 341 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1794 2137 3355 562
Size: 1232 343 1218 3617
Fragments arranged by size:
3617 1232 1218 343
BsiEI CG_ry'CG
Cuts at: 104 582 1871 3952 3998 5206 104
Size: 478 1289 2081 46 1208 1308
Fragments arranged by size:
2081 1308 1289 1208 478 46
BsiHKAI G_wGCw'C
Cuts at: 701 785 800 1712 3424 4202 4392 5184 701
Size: 84 15 912 1712 778 190 792 1927
Fragments arranged by size:
1927 1712 912 792 778 190 84 15
BsmBI CGTCTCn'nnnn_
Cuts at: 285 1007 1749 3270 285
Size: 722 742 1521 3425
Fragments arranged by size:
3425 1521 742 722
Bsp1286I G_dGCh'C
Cuts at: 115 428 701 785 800 893 1372 1673 1712
Size: 313 273 84 15 93 479 301 39
Cuts at: 1712 1739 1906 1964 2671 3209 3424 4035 4128
Size: 27 167 58 707 538 215 611 93
Cuts at: 4128 4202 4392 4454 5184 115
Size: 74 190 62 730 1341
Fragments arranged by size:
1341 730 707 611 538 479 313 301
273 215 190 167 93 93 84 74
62 58 39 27 15
BspEI T'CCGG_A
Cuts at: 857 5630 857
Size: 4773 1637
BspHI T'CATG_A
Cuts at: 314 2991 5586 314
Size: 2677 2595 1138
Fragments arranged by size:
2677 2595 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 534 1292 1302 1904 1941 3460 3674 3976 4357
Size: 758 10 602 37 1519 214 302 381
Cuts at: 4357 534
Size: 2587
Fragments arranged by size:
2587 1519 758 602 381 302 214 37
10
BsrBI CCG'CTC
Cuts at: 50 1794 4702 4756 6240 6405 50
Size: 1744 2908 54 1484 165 55
Fragments arranged by size:
2908 1744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 855 2372 2805 4322 855
Size: 1517 433 1517 2943
Fragments arranged by size:
2943 1517 1517 433
BsrFI r'CCGG_y
Cuts at: 150 530 656 1870 4408 4589 4798 150
Size: 380 126 1214 2538 181 209 1762
Fragments arranged by size:
2538 1762 1214 380 209 181 126
BssSI C'ACGA_G
Cuts at: 4681 5039 4681
Size: 358 6052
BstAPI GCAn_nnn'nTGC
Cuts at: 423 3787 423
Size: 3364 3046
BstBI TT'CG_AA
Cuts at: 4771 4771
Size: 6410
BstEII G'GTnAC_C
Cuts at: 1279 2094 1279
Size: 815 5595
BstXI CCAn_nnnn'nTGG
Cuts at: 1299 2190 2319 1299
Size: 891 129 5390
Fragments arranged by size:
5390 891 129
BstYI r'GATC_y
Cuts at: 1 203 854 1358 1991 2030 3342 3435 3902
Size: 202 651 504 633 39 1312 93 467
Cuts at: 3902 3925 4260 4506 5518 5604 5616 5962 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1312 1012 651 633 504 467 449 346
335 246 202 93 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 610 610
Size: 6410
BtgI C'CryG_G
Cuts at: 725 752 776 1475 1803 1832 725
Size: 27 24 699 328 29 5303
Fragments arranged by size:
5303 699 328 29 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4360 4541 4704 4360
Size: 181 163 6066
Fragments arranged by size:
6066 181 163
BtsI GCAGTG_nn'
Cuts at: 2183 2195 3071 3653 2183
Size: 12 876 582 4940
Fragments arranged by size:
4940 876 582 12
ClaI AT'CG_AT
Cuts at: 878 1256 878
Size: 378 6032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 714 749 714
Size: 35 6375
DraI TTT'AAA
Cuts at: 3409 3409
Size: 6410
DrdI GACnn_nn'nnGTC
Cuts at: 2133 2682 4116 4974 2133
Size: 549 1434 858 3569
Fragments arranged by size:
3569 1434 858 549
EaeI y'GGCC_r
Cuts at: 167 686 977 1451 1868 2309 2499 2894 3995
Size: 519 291 474 417 441 190 395 1101
Cuts at: 3995 4169 4560 4587 5932 167
Size: 174 391 27 1345 645
Fragments arranged by size:
1345 1101 645 519 474 441 417 395
391 291 190 174 27
EagI C'GGCC_G
Cuts at: 1868 3995 1868
Size: 2127 4283
EarI CTCTTCn'nnn_
Cuts at: 1265 1769 2449 4433 4643 1265
Size: 504 680 1984 210 3032
Fragments arranged by size:
3032 1984 680 504 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2000 4618 4928 5074 6220 2000
Size: 2618 310 146 1146 2190
Fragments arranged by size:
2618 2190 1146 310 146
EcoICRI GAG'CTC
Cuts at: 699 783 1710 3422 699
Size: 84 927 1712 3687
Fragments arranged by size:
3687 1712 927 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 313 715 1026 1076 1528 1672 1731 4235
Size: 4 402 311 50 452 144 59 2504
Cuts at: 4235 4667 5414 5682 309
Size: 432 747 268 1037
Fragments arranged by size:
2504 1037 747 452 432 402 311 268
144 59 50 4
EcoNI CCTnn'n_nnAGG
Cuts at: 295 900 2407 2827 3228 295
Size: 605 1507 420 401 3477
Fragments arranged by size:
3477 1507 605 420 401
EcoO109I rG'GnC_Cy
Cuts at: 669 770 890 1842 3169 3549 669
Size: 101 120 952 1327 380 3530
Fragments arranged by size:
3530 1327 952 380 120 101
EcoRI G'AATT_C
Cuts at: 3414 3414
Size: 6410
EcoRV GAT'ATC
Cuts at: 312 312
Size: 6410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4429 4429
Size: 6410
FseI GG_CCGG'CC
Cuts at: 1874 1874
Size: 6410
FspI TGC'GCA
Cuts at: 4191 4191
Size: 6410
HaeII r_GCGC'y
Cuts at: 590 1235 3844 4092 5114 6093 590
Size: 645 2609 248 1022 979 907
Fragments arranged by size:
2609 1022 979 907 645 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 180 212 688 720 1219 1251 1321 1353 1510
Size: 32 476 32 499 32 70 32 157
Cuts at: 1510 1542 1724 1756 2022 2054 3161 3193 4252
Size: 32 182 32 266 32 1107 32 1059
Cuts at: 4252 4282 4284 4314 180
Size: 30 2 30 2276
Fragments arranged by size:
2276 1107 1059 499 476 266 182 157
70 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1608 3449 1608
Size: 1841 4569
KasI G'GCGC_C
Cuts at: 1231 3840 4088 1231
Size: 2609 248 3553
Fragments arranged by size:
3553 2609 248
KpnI G_GTAC'C
Cuts at: 3430 3430
Size: 6410
MluI A'CGCG_T
Cuts at: 5843 5843
Size: 6410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 1708 1724 2451 3008 5081 5265 473
Size: 1235 16 727 557 2073 184 1618
Fragments arranged by size:
2073 1618 1235 727 557 184 16
MscI TGG'CCA
Cuts at: 979 2311 2501 2896 4171 979
Size: 1332 190 395 1275 3218
Fragments arranged by size:
3218 1332 1275 395 190
MslI CAynn'nnrTG
Cuts at: 1108 1951 2077 2188 2990 3304 3398 4399 4526
Size: 843 126 111 802 314 94 1001 127
Cuts at: 4526 1108
Size: 2992
Fragments arranged by size:
2992 1001 843 802 314 127 126 111
94
MspA1I CmG'CkG
Cuts at: 653 710 1180 1207 1299 1398 1683 1805 2121
Size: 57 470 27 92 99 285 122 316
Cuts at: 2121 3153 3521 3835 4195 5208 5453 653
Size: 1032 368 314 360 1013 245 1610
Fragments arranged by size:
1610 1032 1013 470 368 360 316 314
285 245 122 99 92 57 27
NaeI GCC'GGC
Cuts at: 532 658 1872 4591 532
Size: 126 1214 2719 2351
Fragments arranged by size:
2719 2351 1214 126
NarI GG'CG_CC
Cuts at: 1232 3841 4089 1232
Size: 2609 248 3553
Fragments arranged by size:
3553 2609 248
NcoI C'CATG_G
Cuts at: 1832 1832
Size: 6410
NgoMIV G'CCGG_C
Cuts at: 530 656 1870 4589 530
Size: 126 1214 2719 2351
Fragments arranged by size:
2719 2351 1214 126
NspI r_CATG'y
Cuts at: 3463 3963 4870 3463
Size: 500 907 5003
Fragments arranged by size:
5003 907 500
PasI CC'CwG_GG
Cuts at: 802 1876 3352 802
Size: 1074 1476 3860
Fragments arranged by size:
3860 1476 1074
PciI A'CATG_T
Cuts at: 4866 4866
Size: 6410
PflMI CCAn_nnn'nTGG
Cuts at: 561 1899 3345 561
Size: 1338 1446 3626
Fragments arranged by size:
3626 1446 1338
PfoI T'CCnGG_A
Cuts at: 367 367
Size: 6410
PmeI GTTT'AAAC
Cuts at: 3409 3409
Size: 6410
PmlI CAC'GTG
Cuts at: 1307 1307
Size: 6410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5549 5581 5549
Size: 32 6378
PpuMI rG'GwC_Cy
Cuts at: 669 770 1842 3169 669
Size: 101 1072 1327 3910
Fragments arranged by size:
3910 1327 1072 101
PspOMI G'GGCC_C
Cuts at: 889 889
Size: 6410
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1612 1644 1612
Size: 32 6378
PstI C_TGCA'G
Cuts at: 335 665 1688 1820 2190 2210 2696 3457 4142
Size: 330 1023 132 370 20 486 761 685
Cuts at: 4142 335
Size: 2603
Fragments arranged by size:
2603 1023 761 685 486 370 330 132
20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6410
PvuII CAG'CTG
Cuts at: 653 710 1180 1207 1299 2121 3153 3835 4195
Size: 57 470 27 92 822 1032 682 360
Cuts at: 4195 653
Size: 2868
Fragments arranged by size:
2868 1032 822 682 470 360 92 57
27
RsrII CG'GwC_CG
Cuts at: 4605 4605
Size: 6410
SacI G_AGCT'C
Cuts at: 701 785 1712 3424 701
Size: 84 927 1712 3687
Fragments arranged by size:
3687 1712 927 84
SacII CC_GC'GG
Cuts at: 1806 1806
Size: 6410
SalI G'TCGA_C
Cuts at: 3447 3447
Size: 6410
SanDI GG'GwC_CC
Cuts at: 770 3169 770
Size: 2399 4011
SapI GCTCTTCn'nnn_
Cuts at: 1265 4433 4643 1265
Size: 3168 210 3032
Fragments arranged by size:
3168 3032 210
SbfI CC_TGCA'GG
Cuts at: 3457 3457
Size: 6410
ScaI AGT'ACT
Cuts at: 2629 5927 2629
Size: 3298 3112
SfcI C'TryA_G
Cuts at: 33 331 661 1684 1816 2186 2206 2692 3453
Size: 298 330 1023 132 370 20 486 761
Cuts at: 3453 4138 5131 5322 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1023 993 761 685 486 370 330
298 191 132 20
SfoI GGC'GCC
Cuts at: 1233 3842 4090 1233
Size: 2609 248 3553
Fragments arranged by size:
3553 2609 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6410
SmaI CCC'GGG
Cuts at: 340 3432 340
Size: 3092 3318
SmlI C'TyrA_G
Cuts at: 208 3565 4480 4972 5234 208
Size: 3357 915 492 262 1384
Fragments arranged by size:
3357 1384 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6410
SphI G_CATG'C
Cuts at: 3463 3963 3463
Size: 500 5910
StuI AGG'CCT
Cuts at: 1758 1758
Size: 6410
StyI C'CwwG_G
Cuts at: 1832 2273 3544 3898 1832
Size: 441 1271 354 4344
Fragments arranged by size:
4344 1271 441 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 815 1656 3993 4126 4585 4794 815
Size: 841 2337 133 459 209 2431
Fragments arranged by size:
2431 2337 841 459 209 133
TatI w'GTAC_w
Cuts at: 2627 5925 2627
Size: 3298 3112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5927 5959 5927
Size: 32 6378
Tth111I GACn'n_nGTC
Cuts at: 517 823 1570 4207 517
Size: 306 747 2637 2720
Fragments arranged by size:
2720 2637 747 306
XbaI T'CTAG_A
Cuts at: 3441 3441
Size: 6410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 417 2079 2254 3905 417
Size: 1662 175 1651 2922
Fragments arranged by size:
2922 1662 1651 175
XmaI C'CCGG_G
Cuts at: 338 3430 338
Size: 3092 3318
XmnI GAAnn'nnTTC
Cuts at: 2296 2296
Size: 6410
ZraI GAC'GTC
Cuts at: 279 279
Size: 6410
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BfrBI BglI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I DraIII FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI