Restriction map for
pF1KA1953
(Circular) (Six-base) MAPSORT of: pF1KA1953.seq Check: 4736 from: 1 to: 5939
pF1KA1953 5939 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1691 1691
Size: 5939
AatII G_ACGT'C
Cuts at: 899 899
Size: 5939
AccI GT'mk_AC
Cuts at: 280 2977 280
Size: 2697 3242
Acc65I G'GTAC_C
Cuts at: 2955 2955
Size: 5939
AclI AA'CG_TT
Cuts at: 5779 5779
Size: 5939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1422 1750 2698 3764 4196 4549 5211 362
Size: 1060 328 948 1066 432 353 662 1090
Fragments arranged by size:
1090 1066 1060 948 662 432 353 328
AflIII A'CryG_T
Cuts at: 3291 5091 5372 3291
Size: 1800 281 3858
Fragments arranged by size:
3858 1800 281
AgeI A'CCGG_T
Cuts at: 2046 2046
Size: 5939
AhdI GACnn_n'nnGTC
Cuts at: 208 208
Size: 5939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 974 2344 649
Size: 325 1370 4244
Fragments arranged by size:
4244 1370 325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 343 375 343
Size: 32 5907
AlwNI CAG_nnn'CTG
Cuts at: 240 648 1929 2618 2643 3244 4682 240
Size: 408 1281 689 25 601 1438 1497
Fragments arranged by size:
1497 1438 1281 689 601 408 25
ApaI G_GGCC'C
Cuts at: 1236 1923 2684 1236
Size: 687 761 4491
Fragments arranged by size:
4491 761 687
ApaLI G'TGCA_C
Cuts at: 949 1489 4777 949
Size: 540 3288 2111
Fragments arranged by size:
3288 2111 540
ApoI r'AATT_y
Cuts at: 265 866 1717 2943 5292 5548 265
Size: 601 851 1226 2349 256 656
Fragments arranged by size:
2349 1226 851 656 601 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5939
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5939
AvaI C'yCGr_G
Cuts at: 213 1376 1388 2215 2374 2959 213
Size: 1163 12 827 159 585 3193
Fragments arranged by size:
3193 1163 827 585 159 12
BamHI G'GATC_C
Cuts at: 2964 2964
Size: 5939
BanI G'GyrC_C
Cuts at: 602 2286 2556 2955 3369 3617 3652 602
Size: 1684 270 399 414 248 35 2889
Fragments arranged by size:
2889 1684 414 399 270 248 35
BanII G_rGCy'C
Cuts at: 1236 1870 1923 2515 2684 2933 2953 3983 1236
Size: 634 53 592 169 249 20 1030 3192
Fragments arranged by size:
3192 1030 634 592 249 169 53 20
BbeI G_GCGC'C
Cuts at: 606 2560 3373 3621 606
Size: 1954 813 248 2924
Fragments arranged by size:
2924 1954 813 248
BbsI GAAGACnn'nnnn_
Cuts at: 350 1305 1738 2675 350
Size: 955 433 937 3614
Fragments arranged by size:
3614 955 937 433
BbvCI CC'TCA_GC
Cuts at: 1975 1975
Size: 5939
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5905
BciVI GTATCCnnnnn_n'
Cuts at: 3837 4893 5939 3837
Size: 1056 1046 3837
Fragments arranged by size:
3837 1056 1046
BclI T'GATC_A
Cuts at: 2241 3459 2241
Size: 1218 4721
BglI GCCn_nnn'nGGC
Cuts at: 1749 1749
Size: 5939
BglII A'GATC_T
Cuts at: 2365 3454 5491 2365
Size: 1089 2037 2813
Fragments arranged by size:
2813 2037 1089
BlpI GC'TnA_GC
Cuts at: 661 1864 3051 661
Size: 1203 1187 3549
Fragments arranged by size:
3549 1203 1187
Bme1580I G_kGCm'C
Cuts at: 953 1236 1493 1923 2289 2684 3564 3657 4781
Size: 283 257 430 366 395 880 93 1124
Cuts at: 4781 953
Size: 2111
Fragments arranged by size:
2111 1124 880 430 395 366 283 257
93
BmgBI CAC'GTC
Cuts at: 672 1431 672
Size: 759 5180
BmrI ACTGGGnnnn_n'
Cuts at: 2761 3151 3232 3567 5711 2761
Size: 390 81 335 2144 2989
Fragments arranged by size:
2989 2144 390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4306 4306
Size: 5939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 727 1145 1232 1468 1483 1814 1857 2026
Size: 24 418 87 236 15 331 43 169
Cuts at: 2026 2485 703
Size: 459 4157
Fragments arranged by size:
4157 459 418 331 236 169 87 43
24 15
Bpu10I CC'TnA_GC
Cuts at: 1975 2176 1975
Size: 201 5738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2080 3115 3994 4708 5006 1342
Size: 738 1035 879 714 298 2275
Fragments arranged by size:
2275 1035 879 738 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1283 2241 5643 1283
Size: 958 3402 1579
Fragments arranged by size:
3402 1579 958
BsaAI yAC'GTr
Cuts at: 1269 1689 3292 1269
Size: 420 1603 3916
Fragments arranged by size:
3916 1603 420
BsaBI GATnn'nnATC
Cuts at: 6 2147 3453 5490 6
Size: 2141 1306 2037 455
Fragments arranged by size:
2141 2037 1306 455
BsaHI Gr'CG_yC
Cuts at: 603 896 2557 3370 3618 4320 5816 603
Size: 293 1661 813 248 702 1496 726
Fragments arranged by size:
1661 1496 813 726 702 293 248
BsaWI w'CCGG_w
Cuts at: 1121 1292 1893 2046 3128 3649 4327 4738 4885
Size: 171 601 153 1082 521 678 411 147
Cuts at: 4885 5159 1121
Size: 274 1901
Fragments arranged by size:
1901 1082 678 601 521 411 274 171
153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 407 437 1441 1471 2408 2438 407
Size: 30 1004 30 937 30 3908
Fragments arranged by size:
3908 1004 937 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 1246 1510 1513 1543 2023 2081 601
Size: 645 264 3 30 480 58 4459
Fragments arranged by size:
4459 645 480 264 58 30 3
BseYI C'CCAG_C
Cuts at: 120 176 550 641 1582 2456 2631 2683 3364
Size: 56 374 91 941 874 175 52 681
Cuts at: 3364 4122 4787 5195 120
Size: 758 665 408 864
Fragments arranged by size:
941 874 864 758 681 665 408 374
175 91 56 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 1669 2074 1615
Size: 54 405 5480
Fragments arranged by size:
5480 405 54
BsiEI CG_ry'CG
Cuts at: 104 1340 3481 3527 4757 104
Size: 1236 2141 46 1230 1286
Fragments arranged by size:
2141 1286 1236 1230 46
BsiHKAI G_wGCw'C
Cuts at: 623 953 1493 1870 2426 2933 2953 3731 3921
Size: 330 540 377 556 507 20 778 190
Cuts at: 3921 4781 623
Size: 860 1781
Fragments arranged by size:
1781 860 778 556 540 507 377 330
190 20
BsmI GAATG_Cn'
Cuts at: 329 329
Size: 5939
BsmBI CGTCTCn'nnnn_
Cuts at: 1339 1837 1339
Size: 498 5441
Bsp1286I G_dGCh'C
Cuts at: 623 953 1236 1493 1870 1923 2289 2426 2515
Size: 330 283 257 377 53 366 137 89
Cuts at: 2515 2684 2933 2953 3564 3657 3731 3921 3983
Size: 169 249 20 611 93 74 190 62
Cuts at: 3983 4781 623
Size: 798 1781
Fragments arranged by size:
1781 798 611 377 366 330 283 257
249 190 169 137 93 89 74 62
53 20
BspHI T'CATG_A
Cuts at: 4371 4371
Size: 5939
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1691 2989 3203 3505 3886 1691
Size: 1298 214 302 381 3744
Fragments arranged by size:
3744 1298 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1551 1905 4231 4285 5769 5934 50
Size: 1501 354 2326 54 1484 165 55
Fragments arranged by size:
2326 1501 1484 354 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 2588 3851 1646
Size: 942 1263 3734
Fragments arranged by size:
3734 1263 942
BsrFI r'CCGG_y
Cuts at: 1983 2046 2553 3937 4118 1983
Size: 63 507 1384 181 3804
Fragments arranged by size:
3804 1384 507 181 63
BsrGI T'GTAC_A
Cuts at: 1187 1187
Size: 5939
BssSI C'ACGA_G
Cuts at: 1492 4210 4918 1492
Size: 2718 708 2513
Fragments arranged by size:
2718 2513 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2061 3316 2061
Size: 1255 4684
BstBI TT'CG_AA
Cuts at: 4300 4300
Size: 5939
BstEII G'GTnAC_C
Cuts at: 1695 1695
Size: 5939
BstXI CCAn_nnnn'nTGG
Cuts at: 993 1590 2774 993
Size: 597 1184 4158
Fragments arranged by size:
4158 1184 597
BstYI r'GATC_y
Cuts at: 1 1361 2365 2964 3431 3454 3789 4035 4341
Size: 1360 1004 599 467 23 335 246 306
Cuts at: 4341 4353 4439 5491 1
Size: 12 86 1052 449
Fragments arranged by size:
1360 1052 1004 599 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2227 2732 2227
Size: 505 5434
BtgI C'CryG_G
Cuts at: 1334 2016 2033 2622 1334
Size: 682 17 589 4651
Fragments arranged by size:
4651 682 589 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 681 1814 3889 4070 4233 681
Size: 1133 2075 181 163 2387
Fragments arranged by size:
2387 2075 1133 181 163
BtsI GCAGTG_nn'
Cuts at: 548 750 1020 3182 548
Size: 202 270 2162 3305
Fragments arranged by size:
3305 2162 270 202
ClaI AT'CG_AT
Cuts at: 1571 1571
Size: 5939
DraI TTT'AAA
Cuts at: 825 2938 825
Size: 2113 3826
DraIII CAC_nnn'GTG
Cuts at: 2192 2192
Size: 5939
DrdI GACnn_nn'nnGTC
Cuts at: 2257 2633 3645 4989 2257
Size: 376 1012 1344 3207
Fragments arranged by size:
3207 1344 1012 376
EaeI y'GGCC_r
Cuts at: 2304 2910 3524 3698 4089 4116 5461 2304
Size: 606 614 174 391 27 1345 2782
Fragments arranged by size:
2782 1345 614 606 391 174 27
EagI C'GGCC_G
Cuts at: 3524 3524
Size: 5939
EarI CTCTTCn'nnn_
Cuts at: 158 192 451 1066 2798 3962 4172 158
Size: 34 259 615 1732 1164 210 1925
Fragments arranged by size:
1925 1732 1164 615 259 210 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4147 4889 5035 5749 4147
Size: 742 146 714 4337
Fragments arranged by size:
4337 742 714 146
EcoICRI GAG'CTC
Cuts at: 1868 2931 2951 1868
Size: 1063 20 4856
Fragments arranged by size:
4856 1063 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 703 727 1145 1232 1422 1468 1483 1750
Size: 341 24 418 87 190 46 15 267
Cuts at: 1750 1814 1857 2026 2485 2698 3764 4196 4549
Size: 64 43 169 459 213 1066 432 353
Cuts at: 4549 5211 362
Size: 662 1090
Fragments arranged by size:
1090 1066 662 459 432 418 353 341
267 213 190 169 87 64 46 43
24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2008 2647 2008
Size: 639 5300
EcoO109I rG'GnC_Cy
Cuts at: 210 1232 1233 1544 1926 2038 2086 2604 2680
Size: 1022 1 311 382 112 48 518 76
Cuts at: 2680 2728 3078 210
Size: 48 350 3071
Fragments arranged by size:
3071 1022 518 382 350 311 112 76
48 48 1
EcoRI G'AATT_C
Cuts at: 265 2943 265
Size: 2678 3261
EcoRV GAT'ATC
Cuts at: 1151 1151
Size: 5939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1161 3958 1161
Size: 2797 3142
FspI TGC'GCA
Cuts at: 2332 3720 2332
Size: 1388 4551
HaeII r_GCGC'y
Cuts at: 606 2560 3373 3621 4851 5622 606
Size: 1954 813 248 1230 771 923
Fragments arranged by size:
1954 1230 923 813 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 226 866 881 898 913 1382 1414 1441
Size: 32 640 15 17 15 469 32 27
Cuts at: 1441 1473 1495 1527 2078 2110 2355 2357 2387
Size: 32 22 32 551 32 245 2 30
Cuts at: 2387 2389 2408 2440 3781 3811 3813 3843 194
Size: 2 19 32 1341 30 2 30 2290
Fragments arranged by size:
2290 1341 640 551 469 245 32 32
32 32 32 32 30 30 30 27
22 19 17 15 15 2 2 2
HincII GTy'rAC
Cuts at: 2978 2978
Size: 5939
KasI G'GCGC_C
Cuts at: 602 2556 3369 3617 602
Size: 1954 813 248 2924
Fragments arranged by size:
2924 1954 813 248
KpnI G_GTAC'C
Cuts at: 2959 2959
Size: 5939
MluI A'CGCG_T
Cuts at: 5372 5372
Size: 5939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1107 1142 4698 4882 1107
Size: 35 3556 184 2164
Fragments arranged by size:
3556 2164 184 35
MscI TGG'CCA
Cuts at: 2306 2912 3700 2306
Size: 606 788 4545
Fragments arranged by size:
4545 788 606
MslI CAynn'nnrTG
Cuts at: 631 1966 2899 3928 4055 631
Size: 1335 933 1029 127 2515
Fragments arranged by size:
2515 1335 1029 933 127
MspA1I CmG'CkG
Cuts at: 379 648 1917 3050 3364 3724 4508 4753 379
Size: 269 1269 1133 314 360 784 245 1565
Fragments arranged by size:
1565 1269 1133 784 360 314 269 245
NaeI GCC'GGC
Cuts at: 2555 4120 2555
Size: 1565 4374
NarI GG'CG_CC
Cuts at: 603 2557 3370 3618 603
Size: 1954 813 248 2924
Fragments arranged by size:
2924 1954 813 248
NcoI C'CATG_G
Cuts at: 2033 2033
Size: 5939
NdeI CA'TA_TG
Cuts at: 1199 1199
Size: 5939
NgoMIV G'CCGG_C
Cuts at: 2553 4118 2553
Size: 1565 4374
NspI r_CATG'y
Cuts at: 2025 2992 3492 5095 2025
Size: 967 500 1603 2869
Fragments arranged by size:
2869 1603 967 500
PciI A'CATG_T
Cuts at: 5091 5091
Size: 5939
PflMI CCAn_nnn'nTGG
Cuts at: 1929 2442 1929
Size: 513 5426
PfoI T'CCnGG_A
Cuts at: 652 680 652
Size: 28 5911
PmeI GTTT'AAAC
Cuts at: 2938 2938
Size: 5939
PmlI CAC'GTG
Cuts at: 1689 1689
Size: 5939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 5907
PpuMI rG'GwC_Cy
Cuts at: 210 1544 2038 2086 210
Size: 1334 494 48 4063
Fragments arranged by size:
4063 1334 494 48
PspOMI G'GGCC_C
Cuts at: 1232 1919 2680 1232
Size: 687 761 4491
Fragments arranged by size:
4491 761 687
PstI C_TGCA'G
Cuts at: 322 384 543 599 1241 2679 2986 3671 322
Size: 62 159 56 642 1438 307 685 2590
Fragments arranged by size:
2590 1438 685 642 307 159 62 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5939
PvuII CAG'CTG
Cuts at: 379 648 3364 3724 379
Size: 269 2716 360 2594
Fragments arranged by size:
2716 2594 360 269
RsrII CG'GwC_CG
Cuts at: 4134 4134
Size: 5939
SacI G_AGCT'C
Cuts at: 1870 2933 2953 1870
Size: 1063 20 4856
Fragments arranged by size:
4856 1063 20
SalI G'TCGA_C
Cuts at: 2976 2976
Size: 5939
SanDI GG'GwC_CC
Cuts at: 210 2038 210
Size: 1828 4111
SapI GCTCTTCn'nnn_
Cuts at: 2798 3962 4172 2798
Size: 1164 210 4565
Fragments arranged by size:
4565 1164 210
SbfI CC_TGCA'GG
Cuts at: 2679 2986 2679
Size: 307 5632
ScaI AGT'ACT
Cuts at: 5456 5456
Size: 5939
SfcI C'TryA_G
Cuts at: 33 318 380 539 595 833 1237 2675 2982
Size: 285 62 159 56 238 404 1438 307
Cuts at: 2982 3667 4635 4826 33
Size: 685 968 191 1146
Fragments arranged by size:
1438 1146 968 685 404 307 285 238
191 159 62 56
SfoI GGC'GCC
Cuts at: 604 2558 3371 3619 604
Size: 1954 813 248 2924
Fragments arranged by size:
2924 1954 813 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5939
SmaI CCC'GGG
Cuts at: 2217 2961 2217
Size: 744 5195
SmlI C'TyrA_G
Cuts at: 1357 2095 2374 3094 4009 4723 4985 1357
Size: 738 279 720 915 714 262 2311
Fragments arranged by size:
2311 915 738 720 714 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5939
SphI G_CATG'C
Cuts at: 2992 3492 2992
Size: 500 5439
SspI AAT'ATT
Cuts at: 527 527
Size: 5939
StuI AGG'CCT
Cuts at: 1791 1791
Size: 5939
StyI C'CwwG_G
Cuts at: 1049 2033 3073 3427 1049
Size: 984 1040 354 3561
Fragments arranged by size:
3561 1040 984 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 3522 3655 4114 4323 1221
Size: 2301 133 459 209 2837
Fragments arranged by size:
2837 2301 459 209 133
TatI w'GTAC_w
Cuts at: 837 1187 2861 5454 837
Size: 350 1674 2593 1322
Fragments arranged by size:
2593 1674 1322 350
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 407 439 5456 5488 407
Size: 32 5017 32 858
Fragments arranged by size:
5017 858 32 32
Tth111I GACn'n_nGTC
Cuts at: 3736 3736
Size: 5939
XbaI T'CTAG_A
Cuts at: 2970 2970
Size: 5939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 934 1282 3434 934
Size: 348 2152 3439
Fragments arranged by size:
3439 2152 348
XhoI C'TCGA_G
Cuts at: 2374 2374
Size: 5939
XmaI C'CCGG_G
Cuts at: 2215 2959 2215
Size: 744 5195
ZraI GAC'GTC
Cuts at: 897 897
Size: 5939
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI AvrII BaeI BfrBI BmtI
BsiWI BspEI BssHII BstZ17I CspCI FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XmnI