Restriction map for
pF1KA1964
(Circular) (Six-base) MAPSORT of: pF1KA1964.seq Check: 8972 from: 1 to: 5480
pF1KA1964 5480 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2414 2414
Size: 5480
AccI GT'mk_AC
Cuts at: 2518 2518
Size: 5480
Acc65I G'GTAC_C
Cuts at: 2390 2496 2390
Size: 106 5374
AclI AA'CG_TT
Cuts at: 5320 5320
Size: 5480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 880 1546 3305 3737 4090 4752 280
Size: 600 666 1759 432 353 662 1008
Fragments arranged by size:
1759 1008 666 662 600 432 353
AfeI AGC'GCT
Cuts at: 592 2470 592
Size: 1878 3602
AflIII A'CryG_T
Cuts at: 767 1103 1105 2832 4632 4913 767
Size: 336 2 1727 1800 281 1334
Fragments arranged by size:
1800 1727 1334 336 281 2
AleI CACnn'nnGTG
Cuts at: 1106 1108 1106
Size: 2 5478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1442 2119 1442
Size: 677 4803
AlwNI CAG_nnn'CTG
Cuts at: 1482 2106 2121 2265 2785 4223 1482
Size: 624 15 144 520 1438 2739
Fragments arranged by size:
2739 1438 624 520 144 15
ApaI G_GGCC'C
Cuts at: 1199 1199
Size: 5480
ApaLI G'TGCA_C
Cuts at: 4318 4318
Size: 5480
ApoI r'AATT_y
Cuts at: 2484 4833 5089 2484
Size: 2349 256 2875
Fragments arranged by size:
2875 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5480
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
AvaI C'yCGr_G
Cuts at: 145 244 897 1498 1920 2062 2500 145
Size: 99 653 601 422 142 438 3125
Fragments arranged by size:
3125 653 601 438 422 142 99
BamHI G'GATC_C
Cuts at: 665 2505 665
Size: 1840 3640
BanI G'GyrC_C
Cuts at: 189 232 499 567 964 2190 2390 2496 2910
Size: 43 267 68 397 1226 200 106 414
Cuts at: 2910 3158 3193 189
Size: 248 35 2476
Fragments arranged by size:
2476 1226 414 397 267 248 200 106
68 43 35
BanII G_rGCy'C
Cuts at: 155 313 1010 1199 1286 1590 2286 2494 3524
Size: 158 697 189 87 304 696 208 1030
Cuts at: 3524 155
Size: 2111
Fragments arranged by size:
2111 1030 697 696 304 208 189 158
87
BbeI G_GCGC'C
Cuts at: 193 571 968 2914 3162 193
Size: 378 397 1946 248 2511
Fragments arranged by size:
2511 1946 397 378 248
BbvCI CC'TCA_GC
Cuts at: 578 1780 578
Size: 1202 4278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5446
BciVI GTATCCnnnnn_n'
Cuts at: 3378 4434 5480 3378
Size: 1056 1046 3378
Fragments arranged by size:
3378 1056 1046
BclI T'GATC_A
Cuts at: 3000 3000
Size: 5480
BglI GCCn_nnn'nGGC
Cuts at: 973 973
Size: 5480
BglII A'GATC_T
Cuts at: 881 1676 2995 5032 881
Size: 795 1319 2037 1329
Fragments arranged by size:
2037 1329 1319 795
BlpI GC'TnA_GC
Cuts at: 2592 2592
Size: 5480
Bme1580I G_kGCm'C
Cuts at: 924 1199 2195 2208 3105 3198 4322 924
Size: 275 996 13 897 93 1124 2082
Fragments arranged by size:
2082 1124 996 897 275 93 13
BmgBI CAC'GTC
Cuts at: 291 1335 291
Size: 1044 4436
BmrI ACTGGGnnnn_n'
Cuts at: 2692 2773 3108 5252 2692
Size: 81 335 2144 2920
Fragments arranged by size:
2920 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3847 3847
Size: 5480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 895 955 964 1417 1435 144
Size: 751 60 9 453 18 4189
Fragments arranged by size:
4189 751 453 60 18 9
Bpu10I CC'TnA_GC
Cuts at: 382 578 926 1780 382
Size: 196 348 854 4082
Fragments arranged by size:
4082 854 348 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2656 3535 4249 4547 2656
Size: 879 714 298 3589
Fragments arranged by size:
3589 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5184 5184
Size: 5480
BsaAI yAC'GTr
Cuts at: 341 1106 2226 2833 341
Size: 765 1120 607 2988
Fragments arranged by size:
2988 1120 765 607
BsaBI GATnn'nnATC
Cuts at: 6 2994 5031 6
Size: 2988 2037 455
Fragments arranged by size:
2988 2037 455
BsaHI Gr'CG_yC
Cuts at: 190 568 632 965 1954 2327 2911 3159 3861
Size: 378 64 333 989 373 584 248 702
Cuts at: 3861 5357 190
Size: 1496 313
Fragments arranged by size:
1496 989 702 584 378 373 333 313
248 64
BsaWI w'CCGG_w
Cuts at: 2286 2303 2669 3190 3868 4279 4426 4700 2286
Size: 17 366 521 678 411 147 274 3066
Fragments arranged by size:
3066 678 521 411 366 274 147 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 343 373 2317 2347 343
Size: 30 1944 30 3476
Fragments arranged by size:
3476 1944 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 772 853 892 973 1516 1618 1621 1624
Size: 609 81 39 81 543 102 3 3
Cuts at: 1624 1627 1639 1651 163
Size: 3 12 12 3992
Fragments arranged by size:
3992 609 543 102 81 81 39 12
12 3 3 3
BseYI C'CCAG_C
Cuts at: 593 983 1198 1262 1869 2252 2905 3663 4328
Size: 390 215 64 607 383 653 758 665
Cuts at: 4328 4736 593
Size: 408 1337
Fragments arranged by size:
1337 758 665 653 607 408 390 383
215 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 664 1431 1720 2096 460
Size: 204 767 289 376 3844
Fragments arranged by size:
3844 767 376 289 204
BsiEI CG_ry'CG
Cuts at: 104 120 197 816 868 3022 3068 4298 104
Size: 16 77 619 52 2154 46 1230 1286
Fragments arranged by size:
2154 1286 1230 619 77 52 46 16
BsiHKAI G_wGCw'C
Cuts at: 1010 2231 2494 3272 3462 4322 1010
Size: 1221 263 778 190 860 2168
Fragments arranged by size:
2168 1221 860 778 263 190
BsmI GAATG_Cn'
Cuts at: 2139 2139
Size: 5480
Bsp1286I G_dGCh'C
Cuts at: 155 313 924 1010 1199 1286 1590 2195 2208
Size: 158 611 86 189 87 304 605 13
Cuts at: 2208 2231 2286 2494 3105 3198 3272 3462 3524
Size: 23 55 208 611 93 74 190 62
Cuts at: 3524 4322 155
Size: 798 1313
Fragments arranged by size:
1313 798 611 611 605 304 208 190
189 158 93 87 86 74 62 55
23 13
BspEI T'CCGG_A
Cuts at: 2286 2286
Size: 5480
BspHI T'CATG_A
Cuts at: 1055 3912 1055
Size: 2857 2623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2034 2414 2530 2744 3046 3427 2034
Size: 380 116 214 302 381 4087
Fragments arranged by size:
4087 381 380 302 214 116
BsrBI CCG'CTC
Cuts at: 50 332 354 651 3772 3826 5310 5475 50
Size: 282 22 297 3121 54 1484 165 55
Fragments arranged by size:
3121 1484 297 282 165 55 54 22
BsrDI GCAATG_nn'
Cuts at: 3392 3392
Size: 5480
BsrFI r'CCGG_y
Cuts at: 192 362 3478 3659 192
Size: 170 3116 181 2013
Fragments arranged by size:
3116 2013 181 170
BssHII G'CGCG_C
Cuts at: 294 514 620 622 294
Size: 220 106 2 5152
Fragments arranged by size:
5152 220 106 2
BssSI C'ACGA_G
Cuts at: 3751 4459 3751
Size: 708 4772
BstAPI GCAn_nnn'nTGC
Cuts at: 2203 2265 2857 2203
Size: 62 592 4826
Fragments arranged by size:
4826 592 62
BstBI TT'CG_AA
Cuts at: 3841 3841
Size: 5480
BstYI r'GATC_y
Cuts at: 1 326 665 881 1676 2180 2505 2972 2995
Size: 325 339 216 795 504 325 467 23
Cuts at: 2995 3330 3576 3882 3894 3980 5032 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 795 504 467 449 339 335 325
325 306 246 216 86 23 12
BtgI C'CryG_G
Cuts at: 572 1430 2184 572
Size: 858 754 3868
Fragments arranged by size:
3868 858 754
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 404 513 3430 3611 3774 404
Size: 109 2917 181 163 2110
Fragments arranged by size:
2917 2110 181 163 109
BtsI GCAGTG_nn'
Cuts at: 1404 2011 2362 2723 1404
Size: 607 351 361 4161
Fragments arranged by size:
4161 607 361 351
DraI TTT'AAA
Cuts at: 2479 2479
Size: 5480
DrdI GACnn_nn'nnGTC
Cuts at: 3186 4530 3186
Size: 1344 4136
EaeI y'GGCC_r
Cuts at: 117 161 865 1299 1581 1667 3065 3239 3630
Size: 44 704 434 282 86 1398 174 391
Cuts at: 3630 3657 5002 117
Size: 27 1345 595
Fragments arranged by size:
1398 1345 704 595 434 391 282 174
86 44 27
EagI C'GGCC_G
Cuts at: 117 865 3065 117
Size: 748 2200 2532
Fragments arranged by size:
2532 2200 748
EarI CTCTTCn'nnn_
Cuts at: 790 1158 1330 1628 2452 3503 3713 790
Size: 368 172 298 824 1051 210 2557
Fragments arranged by size:
2557 1051 824 368 298 210 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3688 4430 4576 5290 3688
Size: 742 146 714 3878
Fragments arranged by size:
3878 742 714 146
EcoICRI GAG'CTC
Cuts at: 1008 2492 1008
Size: 1484 3996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 280 880 895 955 964 1417 1435 1546
Size: 136 600 15 60 9 453 18 111
Cuts at: 1546 3305 3737 4090 4752 144
Size: 1759 432 353 662 872
Fragments arranged by size:
1759 872 662 600 453 432 353 136
111 60 18 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 277 787 1218 1543 277
Size: 510 431 325 4214
Fragments arranged by size:
4214 510 431 325
EcoO109I rG'GnC_Cy
Cuts at: 480 1195 1223 1536 2074 2423 2619 480
Size: 715 28 313 538 349 196 3341
Fragments arranged by size:
3341 715 538 349 313 196 28
EcoRI G'AATT_C
Cuts at: 2484 2484
Size: 5480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 732 1341 3499 732
Size: 609 2158 2713
Fragments arranged by size:
2713 2158 609
FspI TGC'GCA
Cuts at: 2173 3261 2173
Size: 1088 4392
FspAI rTGC'GCAy
Cuts at: 2173 2173
Size: 5480
HaeII r_GCGC'y
Cuts at: 193 259 492 571 594 968 1483 1989 2472
Size: 66 233 79 23 374 515 506 483
Cuts at: 2472 2914 3162 4392 5163 193
Size: 442 248 1230 771 510
Fragments arranged by size:
1230 771 515 510 506 483 442 374
248 233 79 66 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 905 1495 1527 2317 2349 2410 2442 3322
Size: 32 590 32 790 32 61 32 880
Cuts at: 3322 3352 3354 3384 873
Size: 30 2 30 2969
Fragments arranged by size:
2969 880 790 590 61 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 750 2519 750
Size: 1769 3711
KasI G'GCGC_C
Cuts at: 189 567 964 2910 3158 189
Size: 378 397 1946 248 2511
Fragments arranged by size:
2511 1946 397 378 248
KpnI G_GTAC'C
Cuts at: 2394 2500 2394
Size: 106 5374
MluI A'CGCG_T
Cuts at: 4913 4913
Size: 5480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 719 830 1574 4239 4423 719
Size: 111 744 2665 184 1776
Fragments arranged by size:
2665 1776 744 184 111
MscI TGG'CCA
Cuts at: 1301 1669 3241 1301
Size: 368 1572 3540
Fragments arranged by size:
3540 1572 368
MslI CAynn'nnrTG
Cuts at: 1106 1108 1468 2101 3469 3596 1106
Size: 2 360 633 1368 127 2990
Fragments arranged by size:
2990 1368 633 360 127 2
MspA1I CmG'CkG
Cuts at: 122 399 1244 1524 1564 1851 1869 1932 2121
Size: 277 845 280 40 287 18 63 189
Cuts at: 2121 2252 2277 2591 2905 3265 4049 4294 122
Size: 131 25 314 314 360 784 245 1308
Fragments arranged by size:
1308 845 784 360 314 314 287 280
277 245 189 131 63 40 25 18
NaeI GCC'GGC
Cuts at: 3661 3661
Size: 5480
NarI GG'CG_CC
Cuts at: 190 568 965 2911 3159 190
Size: 378 397 1946 248 2511
Fragments arranged by size:
2511 1946 397 378 248
NcoI C'CATG_G
Cuts at: 1430 2184 1430
Size: 754 4726
NgoMIV G'CCGG_C
Cuts at: 3659 3659
Size: 5480
NotI GC'GGCC_GC
Cuts at: 117 117
Size: 5480
NspI r_CATG'y
Cuts at: 771 1467 2533 3033 4636 771
Size: 696 1066 500 1603 1615
Fragments arranged by size:
1615 1603 1066 696 500
PasI CC'CwG_GG
Cuts at: 1233 1233
Size: 5480
PciI A'CATG_T
Cuts at: 767 4632 767
Size: 3865 1615
PfoI T'CCnGG_A
Cuts at: 941 1115 1328 941
Size: 174 213 5093
Fragments arranged by size:
5093 213 174
PmeI GTTT'AAAC
Cuts at: 2479 2479
Size: 5480
PmlI CAC'GTG
Cuts at: 341 1106 341
Size: 765 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 5448
PpuMI rG'GwC_Cy
Cuts at: 1536 2074 1536
Size: 538 4942
PspOMI G'GGCC_C
Cuts at: 1195 1195
Size: 5480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 754 786 754
Size: 32 5448
PstI C_TGCA'G
Cuts at: 371 1653 2270 2527 3212 371
Size: 1282 617 257 685 2639
Fragments arranged by size:
2639 1282 685 617 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5480
PvuII CAG'CTG
Cuts at: 1524 1851 1932 2121 2277 2905 3265 1524
Size: 327 81 189 156 628 360 3739
Fragments arranged by size:
3739 628 360 327 189 156 81
RsrII CG'GwC_CG
Cuts at: 456 3675 456
Size: 3219 2261
SacI G_AGCT'C
Cuts at: 1010 2494 1010
Size: 1484 3996
SalI G'TCGA_C
Cuts at: 2517 2517
Size: 5480
SapI GCTCTTCn'nnn_
Cuts at: 2452 3503 3713 2452
Size: 1051 210 4219
Fragments arranged by size:
4219 1051 210
SbfI CC_TGCA'GG
Cuts at: 2527 2527
Size: 5480
ScaI AGT'ACT
Cuts at: 895 4997 895
Size: 4102 1378
SfcI C'TryA_G
Cuts at: 33 367 1649 1896 2266 2523 3208 4176 4367
Size: 334 1282 247 370 257 685 968 191
Cuts at: 4367 33
Size: 1146
Fragments arranged by size:
1282 1146 968 685 370 334 257 247
191
SfoI GGC'GCC
Cuts at: 191 569 966 2912 3160 191
Size: 378 397 1946 248 2511
Fragments arranged by size:
2511 1946 397 378 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
SmaI CCC'GGG
Cuts at: 246 2502 246
Size: 2256 3224
SmlI C'TyrA_G
Cuts at: 2635 3550 4264 4526 2635
Size: 915 714 262 3589
Fragments arranged by size:
3589 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5480
SphI G_CATG'C
Cuts at: 2533 3033 2533
Size: 500 4980
SrfI GCCC'GGGC
Cuts at: 246 246
Size: 5480
StuI AGG'CCT
Cuts at: 1213 1213
Size: 5480
StyI C'CwwG_G
Cuts at: 1088 1430 2184 2414 2614 2968 1088
Size: 342 754 230 200 354 3600
Fragments arranged by size:
3600 754 354 342 230 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 585 1114 3063 3196 3655 3864 585
Size: 529 1949 133 459 209 2201
Fragments arranged by size:
2201 1949 529 459 209 133
TatI w'GTAC_w
Cuts at: 893 1706 4995 893
Size: 813 3289 1378
Fragments arranged by size:
3289 1378 813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2395 2427 4997 5029 2395
Size: 32 2570 32 2846
Fragments arranged by size:
2846 2570 32 32
Tth111I GACn'n_nGTC
Cuts at: 1336 3277 1336
Size: 1941 3539
XbaI T'CTAG_A
Cuts at: 819 2511 819
Size: 1692 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2346 2975 2346
Size: 629 4851
XmaI C'CCGG_G
Cuts at: 244 2500 244
Size: 2256 3224
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AloI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PflMI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI SwaI XhoI XmnI ZraI