Restriction map for
pF1KA1966
(Circular) (Six-base) MAPSORT of: pF1KA1966.seq Check: 3101 from: 1 to: 5294
pF1KA1966 5294 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1674 1674
Size: 5294
AccI GT'mk_AC
Cuts at: 1713 2136 2332 1713
Size: 423 196 4675
Fragments arranged by size:
4675 423 196
Acc65I G'GTAC_C
Cuts at: 2310 2310
Size: 5294
AclI AA'CG_TT
Cuts at: 5134 5134
Size: 5294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 656 1340 3119 3551 3904 4566 191
Size: 465 684 1779 432 353 662 919
Fragments arranged by size:
1779 919 684 662 465 432 353
AflIII A'CryG_T
Cuts at: 2646 4446 4727 2646
Size: 1800 281 3213
Fragments arranged by size:
3213 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 2599 4037 2599
Size: 1438 3856
ApaLI G'TGCA_C
Cuts at: 4132 4132
Size: 5294
ApoI r'AATT_y
Cuts at: 449 1330 1891 2298 4647 4903 449
Size: 881 561 407 2349 256 840
Fragments arranged by size:
2349 881 840 561 407 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5294
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
AvaI C'yCGr_G
Cuts at: 2314 2314
Size: 5294
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 158 191 158
Size: 33 5261
BamHI G'GATC_C
Cuts at: 522 1803 2319 522
Size: 1281 516 3497
Fragments arranged by size:
3497 1281 516
BanI G'GyrC_C
Cuts at: 2310 2724 2972 3007 2310
Size: 414 248 35 4597
Fragments arranged by size:
4597 414 248 35
BanII G_rGCy'C
Cuts at: 334 2308 3338 334
Size: 1974 1030 2290
Fragments arranged by size:
2290 1974 1030
BbeI G_GCGC'C
Cuts at: 2728 2976 2728
Size: 248 5046
BbsI GAAGACnn'nnnn_
Cuts at: 605 1567 605
Size: 962 4332
BciVI GTATCCnnnnn_n'
Cuts at: 3192 4248 5294 3192
Size: 1056 1046 3192
Fragments arranged by size:
3192 1056 1046
BclI T'GATC_A
Cuts at: 2814 2814
Size: 5294
BfrBI ATG'CAT
Cuts at: 1647 1647
Size: 5294
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5294
BglII A'GATC_T
Cuts at: 412 964 1282 2809 4846 412
Size: 552 318 1527 2037 860
Fragments arranged by size:
2037 1527 860 552 318
BlpI GC'TnA_GC
Cuts at: 2406 2406
Size: 5294
Bme1580I G_kGCm'C
Cuts at: 2919 3012 4136 2919
Size: 93 1124 4077
Fragments arranged by size:
4077 1124 93
BmgBI CAC'GTC
Cuts at: 592 1531 2215 592
Size: 939 684 3671
Fragments arranged by size:
3671 939 684
BmrI ACTGGGnnnn_n'
Cuts at: 1398 2506 2587 2922 5066 1398
Size: 1108 81 335 2144 1626
Fragments arranged by size:
2144 1626 1108 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 456
Size: 5294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2470 3349 4063 4361 1982
Size: 488 879 714 298 2915
Fragments arranged by size:
2915 879 714 488 298
BsaI GGTCTCn'nnnn_
Cuts at: 866 1779 2144 2162 2252 4998 866
Size: 913 365 18 90 2746 1162
Fragments arranged by size:
2746 1162 913 365 90 18
BsaAI yAC'GTr
Cuts at: 2647 2647
Size: 5294
BsaBI GATnn'nnATC
Cuts at: 6 440 2808 4845 6
Size: 434 2368 2037 455
Fragments arranged by size:
2368 2037 455 434
BsaHI Gr'CG_yC
Cuts at: 1671 1710 2725 2973 3675 5171 1671
Size: 39 1015 248 702 1496 1794
Fragments arranged by size:
1794 1496 1015 702 248 39
BsaWI w'CCGG_w
Cuts at: 512 2483 3004 3682 4093 4240 4514 512
Size: 1971 521 678 411 147 274 1292
Fragments arranged by size:
1971 1292 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 724 811 814 817 826 829 832 841
Size: 582 87 3 3 9 3 3 9
Cuts at: 841 844 847 850 853 898 1985 142
Size: 3 3 3 3 45 1087 3451
Fragments arranged by size:
3451 1087 582 87 45 9 9 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1422 1565 2719 3477 4142 4550 1422
Size: 143 1154 758 665 408 2166
Fragments arranged by size:
2166 1154 758 665 408 143
BsiEI CG_ry'CG
Cuts at: 104 2836 2882 4112 104
Size: 2732 46 1230 1286
Fragments arranged by size:
2732 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 334 2308 3086 3276 4136 334
Size: 1974 778 190 860 1492
Fragments arranged by size:
1974 1492 860 778 190
BsmI GAATG_Cn'
Cuts at: 1647 1649 1934 1647
Size: 2 285 5007
Fragments arranged by size:
5007 285 2
Bsp1286I G_dGCh'C
Cuts at: 334 2308 2919 3012 3086 3276 3338 4136 334
Size: 1974 611 93 74 190 62 798 1492
Fragments arranged by size:
1974 1492 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 512 512
Size: 5294
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 5294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1069 2344 2558 2860 3241 1069
Size: 1275 214 302 381 3122
Fragments arranged by size:
3122 1275 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3586 3640 5124 5289 50
Size: 3536 54 1484 165 55
Fragments arranged by size:
3536 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3206 3206
Size: 5294
BsrFI r'CCGG_y
Cuts at: 3292 3473 3292
Size: 181 5113
BssSI C'ACGA_G
Cuts at: 1664 3565 4273 1664
Size: 1901 708 2685
Fragments arranged by size:
2685 1901 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1929 2671 1929
Size: 742 4552
BstBI TT'CG_AA
Cuts at: 3655 3655
Size: 5294
BstXI CCAn_nnnn'nTGG
Cuts at: 661 1919 661
Size: 1258 4036
BstYI r'GATC_y
Cuts at: 1 412 522 601 964 1282 1375 1803 2319
Size: 411 110 79 363 318 93 428 516
Cuts at: 2319 2786 2809 3144 3390 3696 3708 3794 4846
Size: 467 23 335 246 306 12 86 1052
Cuts at: 4846 1
Size: 449
Fragments arranged by size:
1052 516 467 449 428 411 363 335
318 306 246 110 93 86 79 23
12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3244 3425 3588 3244
Size: 181 163 4950
Fragments arranged by size:
4950 181 163
BtsI GCAGTG_nn'
Cuts at: 675 2537 675
Size: 1862 3432
DraI TTT'AAA
Cuts at: 1437 1799 1862 2293 1437
Size: 362 63 431 4438
Fragments arranged by size:
4438 431 362 63
DraIII CAC_nnn'GTG
Cuts at: 1392 1392
Size: 5294
DrdI GACnn_nn'nnGTC
Cuts at: 3000 4344 3000
Size: 1344 3950
EaeI y'GGCC_r
Cuts at: 2124 2879 3053 3444 3471 4816 2124
Size: 755 174 391 27 1345 2602
Fragments arranged by size:
2602 1345 755 391 174 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5294
EarI CTCTTCn'nnn_
Cuts at: 770 803 818 1006 2280 3317 3527 770
Size: 33 15 188 1274 1037 210 2537
Fragments arranged by size:
2537 1274 1037 210 188 33 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3502 4244 4390 5104 3502
Size: 742 146 714 3692
Fragments arranged by size:
3692 742 714 146
EcoICRI GAG'CTC
Cuts at: 332 2306 332
Size: 1974 3320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 456 656 1340 3119 3551 3904 4566 191
Size: 265 200 684 1779 432 353 662 919
Fragments arranged by size:
1779 919 684 662 432 353 265 200
EcoNI CCTnn'n_nnAGG
Cuts at: 1922 1940 2015 1922
Size: 18 75 5201
Fragments arranged by size:
5201 75 18
EcoO109I rG'GnC_Cy
Cuts at: 1868 2433 1868
Size: 565 4729
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1370 3313 1370
Size: 1943 3351
FspI TGC'GCA
Cuts at: 3075 3075
Size: 5294
HaeII r_GCGC'y
Cuts at: 2728 2976 4206 4977 2728
Size: 248 1230 771 3045
Fragments arranged by size:
3045 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1090 1122 1166 1195 1198 1227 1676 1708 1749
Size: 32 44 29 3 29 449 32 41
Cuts at: 1749 1781 3136 3166 3168 3198 1090
Size: 32 1355 30 2 30 3186
Fragments arranged by size:
3186 1355 449 44 41 32 32 32
30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 1714 2333 1714
Size: 619 4675
KasI G'GCGC_C
Cuts at: 2724 2972 2724
Size: 248 5046
KpnI G_GTAC'C
Cuts at: 2314 2314
Size: 5294
MfeI C'AATT_G
Cuts at: 1034 1034
Size: 5294
MluI A'CGCG_T
Cuts at: 4727 4727
Size: 5294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4053 4237 4053
Size: 184 5110
MscI TGG'CCA
Cuts at: 3055 3055
Size: 5294
MslI CAynn'nnrTG
Cuts at: 1205 2072 3283 3410 1205
Size: 867 1211 127 3089
Fragments arranged by size:
3089 1211 867 127
MspA1I CmG'CkG
Cuts at: 2405 2719 3079 3863 4108 2405
Size: 314 360 784 245 3591
Fragments arranged by size:
3591 784 360 314 245
NaeI GCC'GGC
Cuts at: 3475 3475
Size: 5294
NarI GG'CG_CC
Cuts at: 2725 2973 2725
Size: 248 5046
NcoI C'CATG_G
Cuts at: 107 107
Size: 5294
NdeI CA'TA_TG
Cuts at: 1074 1074
Size: 5294
NgoMIV G'CCGG_C
Cuts at: 3473 3473
Size: 5294
NsiI A_TGCA'T
Cuts at: 1649 1649
Size: 5294
NspI r_CATG'y
Cuts at: 2347 2847 4450 2347
Size: 500 1603 3191
Fragments arranged by size:
3191 1603 500
PciI A'CATG_T
Cuts at: 4446 4446
Size: 5294
PflMI CCAn_nnn'nTGG
Cuts at: 1499 1821 1499
Size: 322 4972
PfoI T'CCnGG_A
Cuts at: 1694 1694
Size: 5294
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 5262
PpuMI rG'GwC_Cy
Cuts at: 1868 1868
Size: 5294
PsiI TTA'TAA
Cuts at: 485 485
Size: 5294
PstI C_TGCA'G
Cuts at: 2341 3026 2341
Size: 685 4609
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5294
PvuII CAG'CTG
Cuts at: 2719 3079 2719
Size: 360 4934
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5294
SacI G_AGCT'C
Cuts at: 334 2308 334
Size: 1974 3320
SalI G'TCGA_C
Cuts at: 1712 2331 1712
Size: 619 4675
SapI GCTCTTCn'nnn_
Cuts at: 3317 3527 3317
Size: 210 5084
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5294
ScaI AGT'ACT
Cuts at: 4811 4811
Size: 5294
SfcI C'TryA_G
Cuts at: 33 2337 3022 3990 4181 33
Size: 2304 685 968 191 1146
Fragments arranged by size:
2304 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2726 2974 2726
Size: 248 5046
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
SmaI CCC'GGG
Cuts at: 2316 2316
Size: 5294
SmlI C'TyrA_G
Cuts at: 1997 2449 3364 4078 4340 1997
Size: 452 915 714 262 2951
Fragments arranged by size:
2951 915 714 452 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5294
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4794
SspI AAT'ATT
Cuts at: 1307 1307
Size: 5294
StyI C'CwwG_G
Cuts at: 107 1498 1753 1918 2428 2782 107
Size: 1391 255 165 510 354 2619
Fragments arranged by size:
2619 1391 510 354 255 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 2276 2877 3010 3469 3678 1380
Size: 896 601 133 459 209 2996
Fragments arranged by size:
2996 896 601 459 209 133
TatI w'GTAC_w
Cuts at: 364 2064 4809 364
Size: 1700 2745 849
Fragments arranged by size:
2745 1700 849
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 5262
Tth111I GACn'n_nGTC
Cuts at: 3091 3091
Size: 5294
XbaI T'CTAG_A
Cuts at: 432 2325 432
Size: 1893 3401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1916 2789 1916
Size: 873 4421
XmaI C'CCGG_G
Cuts at: 2314 2314
Size: 5294
XmnI GAAnn'nnTTC
Cuts at: 1647 1647
Size: 5294
ZraI GAC'GTC
Cuts at: 1672 1672
Size: 5294
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BmtI Bpu10I BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PasI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI