Restriction map for
pF1KA1973
(Circular) (Six-base) MAPSORT of: pF1KA1973.seq Check: 4207 from: 1 to: 6458
pF1KA1973 6458 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2972 3496 2972
Size: 524 5934
Acc65I G'GTAC_C
Cuts at: 3474 3474
Size: 6458
AclI AA'CG_TT
Cuts at: 6298 6298
Size: 6458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 2243 2534 2623 4283 4715 5462 5730 1376
Size: 867 291 89 1660 432 747 268 2104
Fragments arranged by size:
2104 1660 867 747 432 291 268 89
AflIII A'CryG_T
Cuts at: 3102 3810 4914 5891 3102
Size: 708 1104 977 3669
Fragments arranged by size:
3669 1104 977 708
AgeI A'CCGG_T
Cuts at: 4846 4846
Size: 6458
AhdI GACnn_n'nnGTC
Cuts at: 1211 1211
Size: 6458
AleI CACnn'nnGTG
Cuts at: 1081 1081
Size: 6458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1870 2186 1870
Size: 316 6142
AlwNI CAG_nnn'CTG
Cuts at: 146 3384 3763 5330 146
Size: 3238 379 1567 1274
Fragments arranged by size:
3238 1567 1274 379
ApaLI G'TGCA_C
Cuts at: 265 5228 265
Size: 4963 1495
ApoI r'AATT_y
Cuts at: 837 1819 3462 5811 6067 837
Size: 982 1643 2349 256 1228
Fragments arranged by size:
2349 1643 1228 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6458
AvaI C'yCGr_G
Cuts at: 445 2084 3352 3478 445
Size: 1639 1268 126 3425
Fragments arranged by size:
3425 1639 1268 126
BamHI G'GATC_C
Cuts at: 3483 3483
Size: 6458
BanI G'GyrC_C
Cuts at: 189 375 454 3474 3888 4136 4171 189
Size: 186 79 3020 414 248 35 2476
Fragments arranged by size:
3020 2476 414 248 186 79 35
BanII G_rGCy'C
Cuts at: 336 436 734 1229 2909 3272 3353 3472 4502
Size: 100 298 495 1680 363 81 119 1030
Cuts at: 4502 336
Size: 2292
Fragments arranged by size:
2292 1680 1030 495 363 298 119 100
81
BbeI G_GCGC'C
Cuts at: 379 458 3892 4140 379
Size: 79 3434 248 2697
Fragments arranged by size:
3434 2697 248 79
BbsI GAAGACnn'nnnn_
Cuts at: 929 1677 1880 2169 2238 929
Size: 748 203 289 69 5149
Fragments arranged by size:
5149 748 289 203 69
BciVI GTATCCnnnnn_n'
Cuts at: 1120 2716 4356 5117 6458 1120
Size: 1596 1640 761 1341 1120
Fragments arranged by size:
1640 1596 1341 1120 761
BclI T'GATC_A
Cuts at: 767 3978 767
Size: 3211 3247
BfrBI ATG'CAT
Cuts at: 2547 2547
Size: 6458
BglI GCCn_nnn'nGGC
Cuts at: 571 1224 1724 1987 571
Size: 653 500 263 5042
Fragments arranged by size:
5042 653 500 263
BglII A'GATC_T
Cuts at: 2423 3973 6010 2423
Size: 1550 2037 2871
Fragments arranged by size:
2871 2037 1550
BlpI GC'TnA_GC
Cuts at: 136 3570 136
Size: 3434 3024
Bme1580I G_kGCm'C
Cuts at: 194 245 269 1720 1814 1943 4083 4176 5232
Size: 51 24 1451 94 129 2140 93 1056
Cuts at: 5232 194
Size: 1420
Fragments arranged by size:
2140 1451 1420 1056 129 94 93 51
24
BmrI ACTGGGnnnn_n'
Cuts at: 1131 1958 3670 3751 4086 6230 1131
Size: 827 1712 81 335 2144 1359
Fragments arranged by size:
2144 1712 1359 827 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4825 4825
Size: 6458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 3436
Size: 6458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1050 1072 1671 3634 4513 5005 5303 214
Size: 836 22 599 1963 879 492 298 1369
Fragments arranged by size:
1963 1369 879 836 599 492 298 22
BsaI GGTCTCn'nnnn_
Cuts at: 6162 6162
Size: 6458
BsaAI yAC'GTr
Cuts at: 1272 3811 1272
Size: 2539 3919
BsaBI GATnn'nnATC
Cuts at: 6 565 772 3972 6009 6
Size: 559 207 3200 2037 455
Fragments arranged by size:
3200 2037 559 455 207
BsaHI Gr'CG_yC
Cuts at: 376 455 485 2627 3889 4137 4839 6335 376
Size: 79 30 2142 1262 248 702 1496 499
Fragments arranged by size:
2142 1496 1262 702 499 248 79 30
BsaWI w'CCGG_w
Cuts at: 312 3647 4168 4846 5120 5267 5678 312
Size: 3335 521 678 274 147 411 1092
Fragments arranged by size:
3335 1092 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 105 135 457 487 625 655 997 1027 2082
Size: 30 322 30 138 30 342 30 1055
Cuts at: 2082 2112 105
Size: 30 4451
Fragments arranged by size:
4451 1055 342 322 138 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 189 192 597 937 3433 126
Size: 63 3 405 340 2496 3151
Fragments arranged by size:
3151 2496 405 340 63 3
BseYI C'CCAG_C
Cuts at: 193 1173 1348 2142 3219 3883 4641 5218 5714
Size: 980 175 794 1077 664 758 577 496
Cuts at: 5714 193
Size: 937
Fragments arranged by size:
1077 980 937 794 758 664 577 496
175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 2000 2146 739
Size: 1261 146 5051
Fragments arranged by size:
5051 1261 146
BsiEI CG_ry'CG
Cuts at: 104 4000 4046 5254 104
Size: 3896 46 1208 1308
Fragments arranged by size:
3896 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 269 409 734 1268 2966 3272 3353 3472 4250
Size: 140 325 534 1698 306 81 119 778
Cuts at: 4250 4440 5232 269
Size: 190 792 1495
Fragments arranged by size:
1698 1495 792 778 534 325 306 190
140 119 81
Bsp1286I G_dGCh'C
Cuts at: 194 245 269 336 409 436 734 1229 1268
Size: 51 24 67 73 27 298 495 39
Cuts at: 1268 1720 1814 1943 2909 2966 3272 3353 3472
Size: 452 94 129 966 57 306 81 119
Cuts at: 3472 4083 4176 4250 4440 4502 5232 194
Size: 611 93 74 190 62 730 1420
Fragments arranged by size:
1420 966 730 611 495 452 306 298
190 129 119 94 93 81 74 73
67 62 57 51 39 27 24
BspEI T'CCGG_A
Cuts at: 312 5678 312
Size: 5366 1092
BspHI T'CATG_A
Cuts at: 3168 5634 3168
Size: 2466 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3508 3722 4024 4405 3508
Size: 214 302 381 5561
Fragments arranged by size:
5561 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4750 4804 6288 6453 50
Size: 4700 54 1484 165 55
Fragments arranged by size:
4700 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1229 4370 1229
Size: 3141 3317
BsrFI r'CCGG_y
Cuts at: 4456 4637 4846 4456
Size: 181 209 6068
Fragments arranged by size:
6068 209 181
BssSI C'ACGA_G
Cuts at: 784 4729 5087 784
Size: 3945 358 2155
Fragments arranged by size:
3945 2155 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3835 3835
Size: 6458
BstBI TT'CG_AA
Cuts at: 1124 4819 1124
Size: 3695 2763
BstEII G'GTnAC_C
Cuts at: 1659 1659
Size: 6458
BstXI CCAn_nnnn'nTGG
Cuts at: 2064 2129 2324 3105 2064
Size: 65 195 781 5417
Fragments arranged by size:
5417 781 195 65
BstYI r'GATC_y
Cuts at: 1 224 1043 2423 3230 3483 3950 3973 4308
Size: 223 819 1380 807 253 467 23 335
Cuts at: 4308 4554 5566 5652 5664 6010 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1380 1012 819 807 467 449 346 335
253 246 223 86 23 12
BstZ17I GTA'TAC
Cuts at: 2973 2973
Size: 6458
Bsu36I CC'TnA_GG
Cuts at: 1221 1221
Size: 6458
BtgI C'CryG_G
Cuts at: 478 500 622 668 793 1523 478
Size: 22 122 46 125 730 5413
Fragments arranged by size:
5413 730 125 122 46 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4408 4589 4752 4408
Size: 181 163 6114
Fragments arranged by size:
6114 181 163
BtsI GCAGTG_nn'
Cuts at: 636 2168 2514 3701 636
Size: 1532 346 1187 3393
Fragments arranged by size:
3393 1532 1187 346
DraI TTT'AAA
Cuts at: 3457 3457
Size: 6458
DraIII CAC_nnn'GTG
Cuts at: 249 249
Size: 6458
DrdI GACnn_nn'nnGTC
Cuts at: 366 3447 4164 5022 366
Size: 3081 717 858 1802
Fragments arranged by size:
3081 1802 858 717
EaeI y'GGCC_r
Cuts at: 182 299 417 475 563 1594 1725 2119 2303
Size: 117 118 58 88 1031 131 394 184
Cuts at: 2303 4043 4217 4608 4635 5980 182
Size: 1740 174 391 27 1345 660
Fragments arranged by size:
1740 1345 1031 660 394 391 184 174
131 118 117 88 58 27
EagI C'GGCC_G
Cuts at: 4043 4043
Size: 6458
EarI CTCTTCn'nnn_
Cuts at: 1764 2194 2426 3258 4481 4691 1764
Size: 430 232 832 1223 210 3531
Fragments arranged by size:
3531 1223 832 430 232 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 478 1131 4666 4976 5122 6268 478
Size: 653 3535 310 146 1146 668
Fragments arranged by size:
3535 1146 668 653 310 146
EcoICRI GAG'CTC
Cuts at: 732 3270 3351 3470 732
Size: 2538 81 119 3720
Fragments arranged by size:
3720 2538 119 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 2243 2534 2623 3436 4283 4715 5462 5730
Size: 867 291 89 813 847 432 747 268
Cuts at: 5730 1376
Size: 2104
Fragments arranged by size:
2104 867 847 813 747 432 291 268
89
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 2474
Size: 6458
EcoO109I rG'GnC_Cy
Cuts at: 468 1023 2081 3127 3597 468
Size: 555 1058 1046 470 3329
Fragments arranged by size:
3329 1058 1046 555 470
EcoRI G'AATT_C
Cuts at: 3462 3462
Size: 6458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1665 3458 4477 1665
Size: 1793 1019 3646
Fragments arranged by size:
3646 1793 1019
FspI TGC'GCA
Cuts at: 171 4239 171
Size: 4068 2390
HaeII r_GCGC'y
Cuts at: 379 458 694 3892 4140 5162 6141 379
Size: 79 236 3198 248 1022 979 696
Fragments arranged by size:
3198 1022 979 696 248 236 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 792 824 1197 1229 1659 1691 4300
Size: 32 135 32 373 32 430 32 2609
Cuts at: 4300 4330 4332 4362 625
Size: 30 2 30 2721
Fragments arranged by size:
2721 2609 430 373 135 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3497 3497
Size: 6458
KasI G'GCGC_C
Cuts at: 375 454 3888 4136 375
Size: 79 3434 248 2697
Fragments arranged by size:
3434 2697 248 79
KpnI G_GTAC'C
Cuts at: 3478 3478
Size: 6458
MluI A'CGCG_T
Cuts at: 5891 5891
Size: 6458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1350 1653 2305 3418 5129 5313 1350
Size: 303 652 1113 1711 184 2495
Fragments arranged by size:
2495 1711 1113 652 303 184
MscI TGG'CCA
Cuts at: 477 565 1596 1727 2121 2305 4219 477
Size: 88 1031 131 394 184 1914 2716
Fragments arranged by size:
2716 1914 1031 394 184 131 88
MslI CAynn'nnrTG
Cuts at: 763 1081 1369 2074 4447 4574 763
Size: 318 288 705 2373 127 2647
Fragments arranged by size:
2647 2373 705 318 288 127
MspA1I CmG'CkG
Cuts at: 1556 3219 3390 3569 3883 4243 5256 5501 1556
Size: 1663 171 179 314 360 1013 245 2513
Fragments arranged by size:
2513 1663 1013 360 314 245 179 171
NaeI GCC'GGC
Cuts at: 4639 4639
Size: 6458
NarI GG'CG_CC
Cuts at: 376 455 3889 4137 376
Size: 79 3434 248 2697
Fragments arranged by size:
3434 2697 248 79
NcoI C'CATG_G
Cuts at: 622 1523 622
Size: 901 5557
NgoMIV G'CCGG_C
Cuts at: 4637 4637
Size: 6458
NsiI A_TGCA'T
Cuts at: 2549 2549
Size: 6458
NspI r_CATG'y
Cuts at: 3511 4011 4918 3511
Size: 500 907 5051
Fragments arranged by size:
5051 907 500
PciI A'CATG_T
Cuts at: 4914 4914
Size: 6458
PflMI CCAn_nnn'nTGG
Cuts at: 669 1191 669
Size: 522 5936
PmeI GTTT'AAAC
Cuts at: 3457 3457
Size: 6458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1325 1357 5597 5629 1325
Size: 32 4240 32 2154
Fragments arranged by size:
4240 2154 32 32
PpuMI rG'GwC_Cy
Cuts at: 1023 2081 3127 1023
Size: 1058 1046 4354
Fragments arranged by size:
4354 1058 1046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 877 909 877
Size: 32 6426
PstI C_TGCA'G
Cuts at: 3389 3505 4190 3389
Size: 116 685 5657
Fragments arranged by size:
5657 685 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6458
PvuII CAG'CTG
Cuts at: 1556 3219 3390 3883 4243 1556
Size: 1663 171 493 360 3771
Fragments arranged by size:
3771 1663 493 360 171
RsrII CG'GwC_CG
Cuts at: 4653 4653
Size: 6458
SacI G_AGCT'C
Cuts at: 734 3272 3353 3472 734
Size: 2538 81 119 3720
Fragments arranged by size:
3720 2538 119 81
SalI G'TCGA_C
Cuts at: 3495 3495
Size: 6458
SanDI GG'GwC_CC
Cuts at: 3127 3127
Size: 6458
SapI GCTCTTCn'nnn_
Cuts at: 2426 4481 4691 2426
Size: 2055 210 4193
Fragments arranged by size:
4193 2055 210
SbfI CC_TGCA'GG
Cuts at: 3505 3505
Size: 6458
ScaI AGT'ACT
Cuts at: 5975 5975
Size: 6458
SfcI C'TryA_G
Cuts at: 33 1039 1304 3385 3501 4186 5179 5370 33
Size: 1006 265 2081 116 685 993 191 1121
Fragments arranged by size:
2081 1121 1006 993 685 265 191 116
SfoI GGC'GCC
Cuts at: 377 456 3890 4138 377
Size: 79 3434 248 2697
Fragments arranged by size:
3434 2697 248 79
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6458
SmaI CCC'GGG
Cuts at: 447 3480 447
Size: 3033 3425
SmlI C'TyrA_G
Cuts at: 229 1029 1051 1650 3352 3613 4528 5020 5282
Size: 800 22 599 1702 261 915 492 262
Cuts at: 5282 229
Size: 1405
Fragments arranged by size:
1702 1405 915 800 599 492 262 261
22
SnaBI TAC'GTA
Cuts at: 1272 1272
Size: 6458
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6458
SphI G_CATG'C
Cuts at: 3511 4011 3511
Size: 500 5958
SspI AAT'ATT
Cuts at: 1402 1402
Size: 6458
StuI AGG'CCT
Cuts at: 578 1439 578
Size: 861 5597
StyI C'CwwG_G
Cuts at: 622 978 1523 2222 2475 3256 3592 3946 622
Size: 356 545 699 253 781 336 354 3134
Fragments arranged by size:
3134 781 699 545 356 354 336 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1033 1089 1158 1948 2757 4041 4174 4633 4842
Size: 56 69 790 809 1284 133 459 209
Cuts at: 4842 1033
Size: 2649
Fragments arranged by size:
2649 1284 809 790 459 209 133 69
56
TatI w'GTAC_w
Cuts at: 5973 5973
Size: 6458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 5975 6007 457
Size: 32 5486 32 908
Fragments arranged by size:
5486 908 32 32
Tth111I GACn'n_nGTC
Cuts at: 739 3131 4255 739
Size: 2392 1124 2942
Fragments arranged by size:
2942 2392 1124
XbaI T'CTAG_A
Cuts at: 1737 3489 1737
Size: 1752 4706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3953 3953
Size: 6458
XhoI C'TCGA_G
Cuts at: 3352 3352
Size: 6458
XmaI C'CCGG_G
Cuts at: 445 3478 445
Size: 3033 3425
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI SacII
SexAI SfiI SgrAI SrfI SwaI XmnI ZraI