Restriction map for
pF1KA1975
(Circular) (Six-base) MAPSORT of: pF1KA1975.seq Check: 5041 from: 1 to: 4763
pF1KA1975 4763 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1478 1478
Size: 4763
AatII G_ACGT'C
Cuts at: 1649 1649
Size: 4763
AccI GT'mk_AC
Cuts at: 1801 1801
Size: 4763
Acc65I G'GTAC_C
Cuts at: 1779 1779
Size: 4763
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 765 2588 3020 3373 4035 535
Size: 230 1823 432 353 662 1263
Fragments arranged by size:
1823 1263 662 432 353 230
AfeI AGC'GCT
Cuts at: 1679 1679
Size: 4763
AflIII A'CryG_T
Cuts at: 2115 3915 4196 2115
Size: 1800 281 2682
Fragments arranged by size:
2682 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1738 1738
Size: 4763
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1382 1414 1382
Size: 32 4731
AlwNI CAG_nnn'CTG
Cuts at: 1026 1328 1470 1626 2068 3506 1026
Size: 302 142 156 442 1438 2283
Fragments arranged by size:
2283 1438 442 302 156 142
ApaLI G'TGCA_C
Cuts at: 220 3601 220
Size: 3381 1382
ApoI r'AATT_y
Cuts at: 1767 4116 4372 1767
Size: 2349 256 2158
Fragments arranged by size:
2349 2158 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 1655 1783 1655
Size: 128 4635
BamHI G'GATC_C
Cuts at: 1397 1788 1397
Size: 391 4372
BanI G'GyrC_C
Cuts at: 1228 1779 2193 2441 2476 1228
Size: 551 414 248 35 3515
Fragments arranged by size:
3515 551 414 248 35
BanII G_rGCy'C
Cuts at: 1283 1777 2807 1283
Size: 494 1030 3239
Fragments arranged by size:
3239 1030 494
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4515
BbsI GAAGACnn'nnnn_
Cuts at: 523 523
Size: 4763
BbvCI CC'TCA_GC
Cuts at: 911 911
Size: 4763
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3717 4763 2661
Size: 1056 1046 2661
Fragments arranged by size:
2661 1056 1046
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4763
BglI GCCn_nnn'nGGC
Cuts at: 673 1696 673
Size: 1023 3740
BglII A'GATC_T
Cuts at: 169 2278 4315 169
Size: 2109 2037 617
Fragments arranged by size:
2109 2037 617
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4763
Bme1580I G_kGCm'C
Cuts at: 224 2388 2481 3605 224
Size: 2164 93 1124 1382
Fragments arranged by size:
2164 1382 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 782 1975 2056 2391 4535 782
Size: 1193 81 335 2144 1010
Fragments arranged by size:
2144 1193 1010 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 675 780 1276 1686 451
Size: 224 105 496 410 3528
Fragments arranged by size:
3528 496 410 224 105
Bpu10I CC'TnA_GC
Cuts at: 579 911 1282 579
Size: 332 371 4060
Fragments arranged by size:
4060 371 332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3532 3830 1939
Size: 879 714 298 2872
Fragments arranged by size:
2872 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1141 1543 4467 1141
Size: 402 2924 1437
Fragments arranged by size:
2924 1437 402
BsaAI yAC'GTr
Cuts at: 2116 2116
Size: 4763
BsaBI GATnn'nnATC
Cuts at: 6 2277 4314 6
Size: 2271 2037 455
Fragments arranged by size:
2271 2037 455
BsaHI Gr'CG_yC
Cuts at: 1646 2194 2442 3144 4640 1646
Size: 548 248 702 1496 1769
Fragments arranged by size:
1769 1496 702 548 248
BsaWI w'CCGG_w
Cuts at: 1952 2473 3151 3562 3709 3983 1952
Size: 521 678 411 147 274 2732
Fragments arranged by size:
2732 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 253
Size: 30 4733
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 261 1000 1552 261
Size: 739 552 3472
Fragments arranged by size:
3472 739 552
BseYI C'CCAG_C
Cuts at: 289 762 1067 2188 2946 3611 4019 289
Size: 473 305 1121 758 665 408 1033
Fragments arranged by size:
1121 1033 758 665 473 408 305
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1428 1557 1428
Size: 129 4634
BsiEI CG_ry'CG
Cuts at: 104 2305 2351 3581 104
Size: 2201 46 1230 1286
Fragments arranged by size:
2201 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 224 563 1283 1777 2555 2745 3605 224
Size: 339 720 494 778 190 860 1382
Fragments arranged by size:
1382 860 778 720 494 339 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1559 1559
Size: 4763
Bsp1286I G_dGCh'C
Cuts at: 224 563 1283 1777 2388 2481 2555 2745 2807
Size: 339 720 494 611 93 74 190 62
Cuts at: 2807 3605 224
Size: 798 1382
Fragments arranged by size:
1382 798 720 611 494 339 190 93
74 62
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 830 1478 1505 1813 2027 2329 2710 830
Size: 648 27 308 214 302 381 2883
Fragments arranged by size:
2883 648 381 308 302 214 27
BsrBI CCG'CTC
Cuts at: 50 458 990 3055 3109 4593 4758 50
Size: 408 532 2065 54 1484 165 55
Fragments arranged by size:
2065 1484 532 408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 711 1314 2675 711
Size: 603 1361 2799
Fragments arranged by size:
2799 1361 603
BsrFI r'CCGG_y
Cuts at: 2761 2942 2761
Size: 181 4582
BssSI C'ACGA_G
Cuts at: 3034 3742 3034
Size: 708 4055
BstAPI GCAn_nnn'nTGC
Cuts at: 1473 2140 1473
Size: 667 4096
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 653 723 653
Size: 70 4693
BstYI r'GATC_y
Cuts at: 1 169 1022 1397 1788 2255 2278 2613 2859
Size: 168 853 375 391 467 23 335 246
Cuts at: 2859 3165 3177 3263 4315 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 853 467 449 391 375 335 306
246 168 86 23 12
BtgI C'CryG_G
Cuts at: 365 1091 1665 365
Size: 726 574 3463
Fragments arranged by size:
3463 726 574
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2713 2894 3057 2713
Size: 181 163 4419
Fragments arranged by size:
4419 181 163
BtsI GCAGTG_nn'
Cuts at: 329 905 1131 2006 329
Size: 576 226 875 3086
Fragments arranged by size:
3086 875 576 226
DraI TTT'AAA
Cuts at: 257 969 1762 257
Size: 712 793 3258
Fragments arranged by size:
3258 793 712
DraIII CAC_nnn'GTG
Cuts at: 545 1137 545
Size: 592 4171
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3813 2469
Size: 1344 3419
EaeI y'GGCC_r
Cuts at: 655 951 1028 1270 2348 2522 2913 2940 4285
Size: 296 77 242 1078 174 391 27 1345
Cuts at: 4285 655
Size: 1133
Fragments arranged by size:
1345 1133 1078 391 296 242 174 77
27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4763
EarI CTCTTCn'nnn_
Cuts at: 1376 2786 2996 1376
Size: 1410 210 3143
Fragments arranged by size:
3143 1410 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2971 3713 3859 4573 2971
Size: 742 146 714 3161
Fragments arranged by size:
3161 742 714 146
EcoICRI GAG'CTC
Cuts at: 1281 1775 1281
Size: 494 4269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 535 675 765 780 1276 1686 2588 3020
Size: 84 140 90 15 496 410 902 432
Cuts at: 3020 3373 4035 451
Size: 353 662 1179
Fragments arranged by size:
1179 902 662 496 432 410 353 140
90 84 15
EcoNI CCTnn'n_nnAGG
Cuts at: 715 715
Size: 4763
EcoO109I rG'GnC_Cy
Cuts at: 306 1494 1902 306
Size: 1188 408 3167
Fragments arranged by size:
3167 1188 408
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
EcoRV GAT'ATC
Cuts at: 756 756
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4763
FspI TGC'GCA
Cuts at: 681 2544 681
Size: 1863 2900
HaeII r_GCGC'y
Cuts at: 1582 1681 2197 2445 3675 4446 1582
Size: 99 516 248 1230 771 1899
Fragments arranged by size:
1899 1230 771 516 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 126 158 883 915 1140 1172 2605 2635 2637
Size: 32 725 32 225 32 1433 30 2
Cuts at: 2637 2667 126
Size: 30 2222
Fragments arranged by size:
2222 1433 725 225 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1373 1802 1373
Size: 429 4334
HindIII A'AGCT_T
Cuts at: 576 576
Size: 4763
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4515
KpnI G_GTAC'C
Cuts at: 1783 1783
Size: 4763
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 3522 3706 401
Size: 3121 184 1458
Fragments arranged by size:
3121 1458 184
MscI TGG'CCA
Cuts at: 657 953 1030 1272 2524 657
Size: 296 77 242 1252 2896
Fragments arranged by size:
2896 1252 296 242 77
MslI CAynn'nnrTG
Cuts at: 1720 2752 2879 1720
Size: 1032 127 3604
Fragments arranged by size:
3604 1032 127
MspA1I CmG'CkG
Cuts at: 1071 1486 1874 2188 2548 3332 3577 1071
Size: 415 388 314 360 784 245 2257
Fragments arranged by size:
2257 784 415 388 360 314 245
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4763
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4515
NcoI C'CATG_G
Cuts at: 365 1091 365
Size: 726 4037
NdeI CA'TA_TG
Cuts at: 737 775 737
Size: 38 4725
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4763
NspI r_CATG'y
Cuts at: 489 1119 1816 2316 3919 489
Size: 630 697 500 1603 1333
Fragments arranged by size:
1603 1333 697 630 500
PasI CC'CwG_GG
Cuts at: 1458 1458
Size: 4763
PciI A'CATG_T
Cuts at: 3915 3915
Size: 4763
PflMI CCAn_nnn'nTGG
Cuts at: 371 1026 1137 371
Size: 655 111 3997
Fragments arranged by size:
3997 655 111
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4731
PpuMI rG'GwC_Cy
Cuts at: 306 1494 306
Size: 1188 3575
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1188 1220 1188
Size: 32 4731
PstI C_TGCA'G
Cuts at: 1040 1103 1733 1810 2495 1040
Size: 63 630 77 685 3308
Fragments arranged by size:
3308 685 630 77 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 1071 2188 2548 1071
Size: 1117 360 3286
Fragments arranged by size:
3286 1117 360
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1283 1777 1283
Size: 494 4269
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 2786 2996 2786
Size: 210 4553
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SfcI C'TryA_G
Cuts at: 33 1036 1099 1729 1806 2491 3459 3650 33
Size: 1003 63 630 77 685 968 191 1146
Fragments arranged by size:
1146 1003 968 685 630 191 77 63
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4515
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4763
SmlI C'TyrA_G
Cuts at: 1918 2833 3547 3809 1918
Size: 915 714 262 2872
Fragments arranged by size:
2872 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 489 1816 2316 489
Size: 1327 500 2936
Fragments arranged by size:
2936 1327 500
SspI AAT'ATT
Cuts at: 600 600
Size: 4763
StuI AGG'CCT
Cuts at: 1699 1699
Size: 4763
StyI C'CwwG_G
Cuts at: 128 365 716 1091 1897 2251 128
Size: 237 351 375 806 354 2640
Fragments arranged by size:
2640 806 375 354 351 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 357 699 754 2346 2479 2938 3147 357
Size: 342 55 1592 133 459 209 1973
Fragments arranged by size:
1973 1592 459 342 209 133 55
TatI w'GTAC_w
Cuts at: 4278 4278
Size: 4763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4280 4312 4280
Size: 32 4731
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4763
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1117 1176 1222 1515 2258 1117
Size: 59 46 293 743 3622
Fragments arranged by size:
3622 743 293 59 46
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4763
ZraI GAC'GTC
Cuts at: 1647 1647
Size: 4763
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI ApaI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI