Restriction map for
pF1KA1996
(Circular) (Six-base) MAPSORT of: pF1KA1996.seq Check: 7528 from: 1 to: 4361
pF1KA1996 4361 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 843 1017 843
Size: 174 4187
AccI GT'mk_AC
Cuts at: 1399 1399
Size: 4361
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 1279 1355 4201 1279
Size: 76 2846 1439
Fragments arranged by size:
2846 1439 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 1129 2186 2618 2971 3633 563
Size: 566 1057 432 353 662 1291
Fragments arranged by size:
1291 1057 662 566 432 353
AfeI AGC'GCT
Cuts at: 1054 1054
Size: 4361
AflIII A'CryG_T
Cuts at: 1713 3513 3794 1713
Size: 1800 281 2280
Fragments arranged by size:
2280 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1071 1071
Size: 4361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 1152 1161 1666 3104 1152
Size: 9 505 1438 2409
Fragments arranged by size:
2409 1438 505 9
ApaLI G'TGCA_C
Cuts at: 3199 3199
Size: 4361
ApoI r'AATT_y
Cuts at: 441 644 792 958 1365 3714 3970 441
Size: 203 148 166 407 2349 256 832
Fragments arranged by size:
2349 832 407 256 203 166 148
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 1076 1381 1076
Size: 305 4056
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 1377 1791 2039 2074 1377
Size: 414 248 35 3664
Fragments arranged by size:
3664 414 248 35
BanII G_rGCy'C
Cuts at: 1063 1375 2405 1063
Size: 312 1030 3019
Fragments arranged by size:
3019 1030 312
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbvCI CC'TCA_GC
Cuts at: 1220 1220
Size: 4361
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3315 4361 2259
Size: 1056 1046 2259
Fragments arranged by size:
2259 1056 1046
BclI T'GATC_A
Cuts at: 621 1881 621
Size: 1260 3101
BglII A'GATC_T
Cuts at: 270 1876 3913 270
Size: 1606 2037 718
Fragments arranged by size:
2037 1606 718
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3203 1986
Size: 93 1124 3144
Fragments arranged by size:
3144 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1573 1654 1989 4133 1573
Size: 81 335 2144 1801
Fragments arranged by size:
2144 1801 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1102 2728 1102
Size: 1626 2735
Bpu10I CC'TnA_GC
Cuts at: 1220 1220
Size: 4361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1023 1537 2416 3130 3428 1023
Size: 514 879 714 298 1956
Fragments arranged by size:
1956 879 714 514 298
BsaI GGTCTCn'nnnn_
Cuts at: 1230 4065 1230
Size: 2835 1526
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 1792 2040 2742 4238 1792
Size: 248 702 1496 1915
Fragments arranged by size:
1915 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3160 3307 3581 1550
Size: 521 678 411 147 274 2330
Fragments arranged by size:
2330 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 279 309 279
Size: 30 4331
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 908 1208 908
Size: 300 4061
BseYI C'CCAG_C
Cuts at: 1055 1786 2544 3209 3617 1055
Size: 731 758 665 408 1799
Fragments arranged by size:
1799 758 731 665 408
BsiEI CG_ry'CG
Cuts at: 104 1903 1949 3179 104
Size: 1799 46 1230 1286
Fragments arranged by size:
1799 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1375 2153 2343 3203 1375
Size: 778 190 860 2533
Fragments arranged by size:
2533 860 778 190
BsmI GAATG_Cn'
Cuts at: 599 599
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 1063 1375 1986 2079 2153 2343 2405 3203 1063
Size: 312 611 93 74 190 62 798 2221
Fragments arranged by size:
2221 798 611 312 190 93 74 62
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 843 1017 1411 1625 1927 2308 843
Size: 174 394 214 302 381 2896
Fragments arranged by size:
2896 394 381 302 214 174
BsrBI CCG'CTC
Cuts at: 50 2653 2707 4191 4356 50
Size: 2603 54 1484 165 55
Fragments arranged by size:
2603 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1204 2273 1204
Size: 1069 3292
BsrFI r'CCGG_y
Cuts at: 1022 2359 2540 1022
Size: 1337 181 2843
Fragments arranged by size:
2843 1337 181
BssSI C'ACGA_G
Cuts at: 1105 2632 3340 1105
Size: 1527 708 2126
Fragments arranged by size:
2126 1527 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1738 1738
Size: 4361
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 1247 1247
Size: 4361
BstYI r'GATC_y
Cuts at: 1 270 1386 1853 1876 2211 2457 2763 2775
Size: 269 1116 467 23 335 246 306 12
Cuts at: 2775 2861 3913 1
Size: 86 1052 449
Fragments arranged by size:
1116 1052 467 449 335 306 269 246
86 23 12
BtgI C'CryG_G
Cuts at: 785 785
Size: 4361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 339 1604 339
Size: 1265 3096
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 225 260 225
Size: 35 4326
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 3411 2067
Size: 1344 3017
EaeI y'GGCC_r
Cuts at: 1946 2120 2511 2538 3883 1946
Size: 174 391 27 1345 2424
Fragments arranged by size:
2424 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 356 707 969 975 2384 2594 356
Size: 351 262 6 1409 210 2123
Fragments arranged by size:
2123 1409 351 262 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 959 2569 3311 3457 4171 959
Size: 1610 742 146 714 1149
Fragments arranged by size:
1610 1149 742 714 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 1129 2186 2618 2971 3633 563
Size: 566 1057 432 353 662 1291
Fragments arranged by size:
1291 1057 662 566 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1073 1500 1073
Size: 427 3934
EcoRI G'AATT_C
Cuts at: 644 958 1365 644
Size: 314 407 3640
Fragments arranged by size:
3640 407 314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 925 1056 1795 2043 3273 4044 925
Size: 131 739 248 1230 771 1242
Fragments arranged by size:
1242 1230 771 739 248 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 946 978 1057 1089 1203 1235 2203
Size: 32 664 32 79 32 114 32 968
Cuts at: 2203 2233 2235 2265 250
Size: 30 2 30 2346
Fragments arranged by size:
2346 968 664 114 79 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 781 1400 781
Size: 619 3742
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 761 1088 3120 3304 761
Size: 327 2032 184 1818
Fragments arranged by size:
2032 1818 327 184
MscI TGG'CCA
Cuts at: 2122 2122
Size: 4361
MslI CAynn'nnrTG
Cuts at: 1245 2350 2477 1245
Size: 1105 127 3129
Fragments arranged by size:
3129 1105 127
MspA1I CmG'CkG
Cuts at: 917 1472 1786 2146 2930 3175 917
Size: 555 314 360 784 245 2103
Fragments arranged by size:
2103 784 555 360 314 245
NaeI GCC'GGC
Cuts at: 1024 2542 1024
Size: 1518 2843
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NcoI C'CATG_G
Cuts at: 785 785
Size: 4361
NdeI CA'TA_TG
Cuts at: 135 1291 135
Size: 1156 3205
NgoMIV G'CCGG_C
Cuts at: 1022 2540 1022
Size: 1518 2843
NspI r_CATG'y
Cuts at: 1134 1414 1914 3517 1134
Size: 280 500 1603 1978
Fragments arranged by size:
1978 1603 500 280
PciI A'CATG_T
Cuts at: 3513 3513
Size: 4361
PflMI CCAn_nnn'nTGG
Cuts at: 1292 1292
Size: 4361
PfoI T'CCnGG_A
Cuts at: 825 825
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 4329
PpuMI rG'GwC_Cy
Cuts at: 1073 1073
Size: 4361
PstI C_TGCA'G
Cuts at: 1124 1157 1408 2093 1124
Size: 33 251 685 3392
Fragments arranged by size:
3392 685 251 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 1786 2146 1786
Size: 360 4001
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SanDI GG'GwC_CC
Cuts at: 1073 1073
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SfcI C'TryA_G
Cuts at: 33 1120 1153 1404 2089 3057 3248 33
Size: 1087 33 251 685 968 191 1146
Fragments arranged by size:
1146 1087 968 685 251 191 33
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 1002 1516 2431 3145 3407 1002
Size: 514 915 714 262 1956
Fragments arranged by size:
1956 915 714 514 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 785 1016 1495 1849 785
Size: 231 479 354 3297
Fragments arranged by size:
3297 479 354 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 1944 2077 2536 2745 754
Size: 1190 133 459 209 2370
Fragments arranged by size:
2370 1190 459 209 133
TatI w'GTAC_w
Cuts at: 3876 3876
Size: 4361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 3878 3910 279
Size: 32 3567 32 730
Fragments arranged by size:
3567 730 32 32
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 783 1856 783
Size: 1073 3288
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
XmnI GAAnn'nnTTC
Cuts at: 812 812
Size: 4361
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BpmI
BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI