Restriction map for
pF1KA1998
(Circular) (Six-base) MAPSORT of: pF1KA1998.seq Check: 5362 from: 1 to: 8228
pF1KA1998 8228 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4466 4466
Size: 8228
AatII G_ACGT'C
Cuts at: 761 3863 761
Size: 3102 5126
AccI GT'mk_AC
Cuts at: 1226 4937 5266 1226
Size: 3711 329 4188
Fragments arranged by size:
4188 3711 329
Acc65I G'GTAC_C
Cuts at: 2389 5244 2389
Size: 2855 5373
AclI AA'CG_TT
Cuts at: 266 8068 266
Size: 7802 426
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 283 1031 1445 2391 2715 3701 3752 4249
Size: 74 748 414 946 324 986 51 497
Cuts at: 4249 4420 6053 6485 6838 7500 209
Size: 171 1633 432 353 662 937
Fragments arranged by size:
1633 986 946 937 748 662 497 432
414 353 324 171 74 51
AfeI AGC'GCT
Cuts at: 3197 3197
Size: 8228
AflIII A'CryG_T
Cuts at: 234 3324 5580 7380 7661 234
Size: 3090 2256 1800 281 801
Fragments arranged by size:
3090 2256 1800 801 281
AhdI GACnn_n'nnGTC
Cuts at: 2662 4383 2662
Size: 1721 6507
AleI CACnn'nnGTG
Cuts at: 2145 2145
Size: 8228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3842 3842
Size: 8228
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1623 1655 1623
Size: 32 8196
AlwNI CAG_nnn'CTG
Cuts at: 459 1009 1948 2709 3345 4644 4686 4996 5533
Size: 550 939 761 636 1299 42 310 537
Cuts at: 5533 6971 459
Size: 1438 1716
Fragments arranged by size:
1716 1438 1299 939 761 636 550 537
310 42
ApaI G_GGCC'C
Cuts at: 4677 4677
Size: 8228
ApaLI G'TGCA_C
Cuts at: 2170 4426 7066 2170
Size: 2256 2640 3332
Fragments arranged by size:
3332 2640 2256
ApoI r'AATT_y
Cuts at: 765 1026 1796 1898 2212 2259 2783 2880 2965
Size: 261 770 102 314 47 524 97 85
Cuts at: 2965 3033 3080 3608 3749 3791 4194 4239 5100
Size: 68 47 528 141 42 403 45 861
Cuts at: 5100 5232 7581 7837 765
Size: 132 2349 256 1156
Fragments arranged by size:
2349 1156 861 770 528 524 403 314
261 256 141 132 102 97 85 68
47 47 45 42
AseI AT'TA_AT
Cuts at: 20 1280 20
Size: 1260 6968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8228
AvaI C'yCGr_G
Cuts at: 663 797 4129 4750 5248 663
Size: 134 3332 621 498 3643
Fragments arranged by size:
3643 3332 621 498 134
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2905 2938 2905
Size: 33 8195
BamHI G'GATC_C
Cuts at: 2613 3653 4125 4133 5253 2613
Size: 1040 472 8 1120 5588
Fragments arranged by size:
5588 1120 1040 472 8
BanI G'GyrC_C
Cuts at: 703 2389 3988 5244 5658 5906 5941 703
Size: 1686 1599 1256 414 248 35 2990
Fragments arranged by size:
2990 1686 1599 1256 414 248 35
BanII G_rGCy'C
Cuts at: 364 515 1624 2500 3875 4677 5242 6272 364
Size: 151 1109 876 1375 802 565 1030 2320
Fragments arranged by size:
2320 1375 1109 1030 876 802 565 151
BbeI G_GCGC'C
Cuts at: 3992 5662 5910 3992
Size: 1670 248 6310
Fragments arranged by size:
6310 1670 248
BbsI GAAGACnn'nnnn_
Cuts at: 761 1115 3722 761
Size: 354 2607 5267
Fragments arranged by size:
5267 2607 354
BbvCI CC'TCA_GC
Cuts at: 2563 4627 2563
Size: 2064 6164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 412 446 685 719 412
Size: 34 239 34 7921
Fragments arranged by size:
7921 239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6126 7182 8228 6126
Size: 1056 1046 6126
Fragments arranged by size:
6126 1056 1046
BclI T'GATC_A
Cuts at: 1248 3495 3572 5748 1248
Size: 2247 77 2176 3728
Fragments arranged by size:
3728 2247 2176 77
BfrBI ATG'CAT
Cuts at: 2694 3092 2694
Size: 398 7830
BglI GCCn_nnn'nGGC
Cuts at: 481 3742 4691 481
Size: 3261 949 4018
Fragments arranged by size:
4018 3261 949
BglII A'GATC_T
Cuts at: 2726 5743 7780 2726
Size: 3017 2037 3174
Fragments arranged by size:
3174 3017 2037
BlpI GC'TnA_GC
Cuts at: 5340 5340
Size: 8228
Bme1580I G_kGCm'C
Cuts at: 2174 4430 4677 5853 5946 7070 2174
Size: 2256 247 1176 93 1124 3332
Fragments arranged by size:
3332 2256 1176 1124 247 93
BmrI ACTGGGnnnn_n'
Cuts at: 639 1373 1643 5440 5521 5856 8000 639
Size: 734 270 3797 81 335 2144 867
Fragments arranged by size:
3797 2144 867 734 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 823 6595 823
Size: 5772 2456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 978 1592 1683 1778 2995 4303 4432 4576
Size: 722 614 91 95 1217 1308 129 144
Cuts at: 4576 4582 4639 5210 256
Size: 6 57 571 3274
Fragments arranged by size:
3274 1308 1217 722 614 571 144 129
95 91 57 6
Bpu10I CC'TnA_GC
Cuts at: 2563 4627 2563
Size: 2064 6164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1611 3292 4927 5404 6283 6997 7295 1611
Size: 1681 1635 477 879 714 298 2544
Fragments arranged by size:
2544 1681 1635 879 714 477 298
BsaI GGTCTCn'nnnn_
Cuts at: 1619 2296 2471 7932 1619
Size: 677 175 5461 1915
Fragments arranged by size:
5461 1915 677 175
BsaAI yAC'GTr
Cuts at: 381 730 5581 381
Size: 349 4851 3028
Fragments arranged by size:
4851 3028 349
BsaBI GATnn'nnATC
Cuts at: 6 151 1130 4124 5742 7779 6
Size: 145 979 2994 1618 2037 455
Fragments arranged by size:
2994 2037 1618 979 455 145
BsaHI Gr'CG_yC
Cuts at: 758 1556 3072 3860 3989 5659 5907 6609 8105
Size: 798 1516 788 129 1670 248 702 1496
Cuts at: 8105 758
Size: 881
Fragments arranged by size:
1670 1516 1496 881 798 788 702 248
129
BsaWI w'CCGG_w
Cuts at: 348 911 1683 5417 5938 6616 7027 7174 7448
Size: 563 772 3734 521 678 411 147 274
Cuts at: 7448 348
Size: 1128
Fragments arranged by size:
3734 1128 772 678 563 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4013 4043 4321 4351 4525 4531 4555 4561 4013
Size: 30 278 30 174 6 24 6 7680
Fragments arranged by size:
7680 278 174 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 1614 2764 3568 3823 4426 4570 4731 523
Size: 1091 1150 804 255 603 144 161 4020
Fragments arranged by size:
4020 1150 1091 804 603 255 161 144
BseYI C'CCAG_C
Cuts at: 1430 4676 4689 5653 6411 7076 7484 1430
Size: 3246 13 964 758 665 408 2174
Fragments arranged by size:
3246 2174 964 758 665 408 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1022 823
Size: 199 8029
BsiEI CG_ry'CG
Cuts at: 104 2895 4710 5770 5816 7046 104
Size: 2791 1815 1060 46 1230 1286
Fragments arranged by size:
2791 1815 1286 1230 1060 46
BsiHKAI G_wGCw'C
Cuts at: 492 515 1624 2174 4430 4736 5242 6020 6210
Size: 23 1109 550 2256 306 506 778 190
Cuts at: 6210 7070 492
Size: 860 1650
Fragments arranged by size:
2256 1650 1109 860 778 550 506 306
190 23
BsmI GAATG_Cn'
Cuts at: 1578 3092 3094 1578
Size: 1514 2 6712
Fragments arranged by size:
6712 1514 2
BsmBI CGTCTCn'nnnn_
Cuts at: 298 3642 4167 4255 298
Size: 3344 525 88 4271
Fragments arranged by size:
4271 3344 525 88
Bsp1286I G_dGCh'C
Cuts at: 364 492 515 1624 2174 2500 3875 4430 4677
Size: 128 23 1109 550 326 1375 555 247
Cuts at: 4677 4736 5242 5853 5946 6020 6210 6272 7070
Size: 59 506 611 93 74 190 62 798
Cuts at: 7070 364
Size: 1522
Fragments arranged by size:
1522 1375 1109 798 611 555 550 506
326 247 190 128 93 74 62 59
23
BspEI T'CCGG_A
Cuts at: 911 911
Size: 8228
BspHI T'CATG_A
Cuts at: 239 1985 4053 4788 6660 239
Size: 1746 2068 735 1872 1807
Fragments arranged by size:
2068 1872 1807 1746 735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1941 2453 4466 5278 5492 5794 6175 1941
Size: 512 2013 812 214 302 381 3994
Fragments arranged by size:
3994 2013 812 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3848 4530 4536 4623 6520 6574 8058 8223
Size: 3798 682 6 87 1897 54 1484 165
Cuts at: 8223 50
Size: 55
Fragments arranged by size:
3798 1897 1484 682 165 87 55 54
6
BsrDI GCAATG_nn'
Cuts at: 1174 3763 6140 1174
Size: 2589 2377 3262
Fragments arranged by size:
3262 2589 2377
BsrFI r'CCGG_y
Cuts at: 1190 4709 6226 6407 1190
Size: 3519 1517 181 3011
Fragments arranged by size:
3519 3011 1517 181
BssSI C'ACGA_G
Cuts at: 514 6499 7207 514
Size: 5985 708 1535
Fragments arranged by size:
5985 1535 708
BstAPI GCAn_nnn'nTGC
Cuts at: 496 4686 5605 496
Size: 4190 919 3119
Fragments arranged by size:
4190 3119 919
BstBI TT'CG_AA
Cuts at: 4966 6589 4966
Size: 1623 6605
BstEII G'GTnAC_C
Cuts at: 351 4146 351
Size: 3795 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 750 2427 750
Size: 1677 6551
BstYI r'GATC_y
Cuts at: 1 220 2613 2726 3275 3653 4125 4133 4301
Size: 219 2393 113 549 378 472 8 168
Cuts at: 4301 5253 5720 5743 6078 6324 6630 6642 6728
Size: 952 467 23 335 246 306 12 86
Cuts at: 6728 7780 1
Size: 1052 449
Fragments arranged by size:
2393 1052 952 549 472 467 449 378
335 306 246 219 168 113 86 23
12 8
BtgI C'CryG_G
Cuts at: 107 291 356 2492 2774 4727 107
Size: 184 65 2136 282 1953 3608
Fragments arranged by size:
3608 2136 1953 282 184 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6178 6359 6522 6178
Size: 181 163 7884
Fragments arranged by size:
7884 181 163
BtsI GCAGTG_nn'
Cuts at: 490 1295 1747 2107 2140 2152 2574 3974 5471
Size: 805 452 360 33 12 422 1400 1497
Cuts at: 5471 490
Size: 3247
Fragments arranged by size:
3247 1497 1400 805 452 422 360 33
12
ClaI AT'CG_AT
Cuts at: 4770 4770
Size: 8228
DraI TTT'AAA
Cuts at: 3003 3236 5227 3003
Size: 233 1991 6004
Fragments arranged by size:
6004 1991 233
DraIII CAC_nnn'GTG
Cuts at: 1334 1396 1334
Size: 62 8166
DrdI GACnn_nn'nnGTC
Cuts at: 4159 5934 7278 4159
Size: 1775 1344 5109
Fragments arranged by size:
5109 1775 1344
EaeI y'GGCC_r
Cuts at: 3743 4157 4344 4418 4707 5004 5813 5987 6378
Size: 414 187 74 289 297 809 174 391
Cuts at: 6378 6405 7750 3743
Size: 27 1345 4221
Fragments arranged by size:
4221 1345 809 414 391 297 289 187
174 74 27
EagI C'GGCC_G
Cuts at: 4707 5813 4707
Size: 1106 7122
EarI CTCTTCn'nnn_
Cuts at: 608 692 913 1010 1847 2505 4101 6251 6461
Size: 84 221 97 837 658 1596 2150 210
Cuts at: 6461 608
Size: 2375
Fragments arranged by size:
2375 2150 1596 837 658 221 210 97
84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4051 6436 7178 7324 8038 4051
Size: 2385 742 146 714 4241
Fragments arranged by size:
4241 2385 742 714 146
EcoICRI GAG'CTC
Cuts at: 513 1622 5240 513
Size: 1109 3618 3501
Fragments arranged by size:
3618 3501 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 256 283 978 1031 1445 1592 1683 1778
Size: 47 27 695 53 414 147 91 95
Cuts at: 1778 2391 2715 2995 3701 3752 4249 4303 4420
Size: 613 324 280 706 51 497 54 117
Cuts at: 4420 4432 4576 4582 4639 5210 6053 6485 6838
Size: 12 144 6 57 571 843 432 353
Cuts at: 6838 7500 209
Size: 662 937
Fragments arranged by size:
937 843 706 695 662 613 571 497
432 414 353 324 280 147 144 117
95 91 57 54 53 51 47 27
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3896 3896
Size: 8228
EcoO109I rG'GnC_Cy
Cuts at: 627 4332 5367 627
Size: 3705 1035 3488
Fragments arranged by size:
3705 3488 1035
EcoRI G'AATT_C
Cuts at: 1026 1898 2965 3080 5232 1026
Size: 872 1067 115 2152 4022
Fragments arranged by size:
4022 2152 1067 872 115
EcoRV GAT'ATC
Cuts at: 1146 1269 3420 1146
Size: 123 2151 5954
Fragments arranged by size:
5954 2151 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 151 6247 151
Size: 6096 2132
FspI TGC'GCA
Cuts at: 4474 6009 4474
Size: 1535 6693
HaeII r_GCGC'y
Cuts at: 3199 3992 4467 4644 5662 5910 7140 7911 3199
Size: 793 475 177 1018 248 1230 771 3516
Fragments arranged by size:
3516 1230 1018 793 771 475 248 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1989 2021 2648 2680 4013 4045 4321 4353 4369
Size: 32 627 32 1333 32 276 32 16
Cuts at: 4369 4401 4531 4563 4770 4802 6070 6100 6102
Size: 32 130 32 207 32 1268 30 2
Cuts at: 6102 6132 1989
Size: 30 4085
Fragments arranged by size:
4085 1333 1268 627 276 207 130 32
32 32 32 32 32 32 30 30
16 2
HincII GTy'rAC
Cuts at: 2994 5267 2994
Size: 2273 5955
HindIII A'AGCT_T
Cuts at: 789 1862 2403 3526 4814 789
Size: 1073 541 1123 1288 4203
Fragments arranged by size:
4203 1288 1123 1073 541
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 8228
KasI G'GCGC_C
Cuts at: 3988 5658 5906 3988
Size: 1670 248 6310
Fragments arranged by size:
6310 1670 248
KpnI G_GTAC'C
Cuts at: 2393 5248 2393
Size: 2855 5373
MluI A'CGCG_T
Cuts at: 7661 7661
Size: 8228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 869 1493 2304 6987 7171 869
Size: 624 811 4683 184 1926
Fragments arranged by size:
4683 1926 811 624 184
MscI TGG'CCA
Cuts at: 3745 4420 5989 3745
Size: 675 1569 5984
Fragments arranged by size:
5984 1569 675
MslI CAynn'nnrTG
Cuts at: 2145 2474 3824 4874 6217 6344 2145
Size: 329 1350 1050 1343 127 4029
Fragments arranged by size:
4029 1350 1343 1050 329 127
MspA1I CmG'CkG
Cuts at: 998 2453 2760 4461 4494 4729 4996 5339 5653
Size: 1455 307 1701 33 235 267 343 314
Cuts at: 5653 6013 6797 7042 998
Size: 360 784 245 2184
Fragments arranged by size:
2184 1701 1455 784 360 343 314 307
267 245 235 33
NaeI GCC'GGC
Cuts at: 1192 4711 6409 1192
Size: 3519 1698 3011
Fragments arranged by size:
3519 3011 1698
NarI GG'CG_CC
Cuts at: 3989 5659 5907 3989
Size: 1670 248 6310
Fragments arranged by size:
6310 1670 248
NcoI C'CATG_G
Cuts at: 107 291 356 2492 107
Size: 184 65 2136 5843
Fragments arranged by size:
5843 2136 184 65
NgoMIV G'CCGG_C
Cuts at: 1190 4709 6407 1190
Size: 3519 1698 3011
Fragments arranged by size:
3519 3011 1698
NruI TCG'CGA
Cuts at: 4391 4391
Size: 8228
NsiI A_TGCA'T
Cuts at: 2696 3094 2696
Size: 398 7830
NspI r_CATG'y
Cuts at: 238 1206 2204 3328 5281 5781 7384 238
Size: 968 998 1124 1953 500 1603 1082
Fragments arranged by size:
1953 1603 1124 1082 998 968 500
PacI TTA_AT'TAA
Cuts at: 3209 3209
Size: 8228
PciI A'CATG_T
Cuts at: 234 3324 7380 234
Size: 3090 4056 1082
Fragments arranged by size:
4056 3090 1082
PflMI CCAn_nnn'nTGG
Cuts at: 292 681 2287 4143 4311 4644 292
Size: 389 1606 1856 168 333 3876
Fragments arranged by size:
3876 1856 1606 389 333 168
PfoI T'CCnGG_A
Cuts at: 4598 4598
Size: 8228
PmeI GTTT'AAAC
Cuts at: 5227 5227
Size: 8228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6674 6706 6674
Size: 32 8196
PsiI TTA'TAA
Cuts at: 5131 5131
Size: 8228
PspOMI G'GGCC_C
Cuts at: 4673 4673
Size: 8228
PstI C_TGCA'G
Cuts at: 124 448 1188 2102 2147 2458 2759 4526 5123
Size: 324 740 914 45 311 301 1767 597
Cuts at: 5123 5275 5960 124
Size: 152 685 2392
Fragments arranged by size:
2392 1767 914 740 685 597 324 311
301 152 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8228
PvuII CAG'CTG
Cuts at: 2453 2760 5653 6013 2453
Size: 307 2893 360 4668
Fragments arranged by size:
4668 2893 360 307
RsrII CG'GwC_CG
Cuts at: 6423 6423
Size: 8228
SacI G_AGCT'C
Cuts at: 515 1624 5242 515
Size: 1109 3618 3501
Fragments arranged by size:
3618 3501 1109
SacII CC_GC'GG
Cuts at: 4730 4730
Size: 8228
SalI G'TCGA_C
Cuts at: 5265 5265
Size: 8228
SapI GCTCTTCn'nnn_
Cuts at: 1847 2505 6251 6461 1847
Size: 658 3746 210 3614
Fragments arranged by size:
3746 3614 658 210
SbfI CC_TGCA'GG
Cuts at: 5275 5275
Size: 8228
ScaI AGT'ACT
Cuts at: 7745 7745
Size: 8228
SfcI C'TryA_G
Cuts at: 33 120 444 1184 1364 1732 2098 2143 2454
Size: 87 324 740 180 368 366 45 311
Cuts at: 2454 2485 2510 2755 2832 3401 3665 4522 5119
Size: 31 25 245 77 569 264 857 597
Cuts at: 5119 5271 5956 6924 7115 33
Size: 152 685 968 191 1146
Fragments arranged by size:
1146 968 857 740 685 597 569 368
366 324 311 264 245 191 180 152
87 77 45 31 25
SfoI GGC'GCC
Cuts at: 3990 5660 5908 3990
Size: 1670 248 6310
Fragments arranged by size:
6310 1670 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8228
SmaI CCC'GGG
Cuts at: 665 4131 5250 665
Size: 3466 1119 3643
Fragments arranged by size:
3643 3466 1119
SmlI C'TyrA_G
Cuts at: 1626 3271 4942 5383 6298 7012 7274 1626
Size: 1645 1671 441 915 714 262 2580
Fragments arranged by size:
2580 1671 1645 915 714 441 262
SpeI A'CTAG_T
Cuts at: 67 4921 67
Size: 4854 3374
SphI G_CATG'C
Cuts at: 2204 5281 5781 2204
Size: 3077 500 4651
Fragments arranged by size:
4651 3077 500
SspI AAT'ATT
Cuts at: 5211 5211
Size: 8228
StuI AGG'CCT
Cuts at: 679 949 3976 679
Size: 270 3027 4931
Fragments arranged by size:
4931 3027 270
StyI C'CwwG_G
Cuts at: 107 291 356 500 680 1550 2253 2396 2426
Size: 184 65 144 180 870 703 143 30
Cuts at: 2426 2492 3623 5362 5716 107
Size: 66 1131 1739 354 2619
Fragments arranged by size:
2619 1739 1131 870 703 354 184 180
144 143 66 65 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1806 2909 5811 5944 6403 6612 1806
Size: 1103 2902 133 459 209 3422
Fragments arranged by size:
3422 2902 1103 459 209 133
TatI w'GTAC_w
Cuts at: 3152 4821 7743 3152
Size: 1669 2922 3637
Fragments arranged by size:
3637 2922 1669
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1629 1661 2234 2266 2293 2325 3604 3636 4125
Size: 32 573 32 27 32 1279 32 489
Cuts at: 4125 4157 4525 4557 5062 5094 7745 7777 1629
Size: 32 368 32 505 32 2651 32 2080
Fragments arranged by size:
2651 2080 1279 573 505 489 368 32
32 32 32 32 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 4027 6025 4027
Size: 1998 6230
XbaI T'CTAG_A
Cuts at: 546 3867 5259 546
Size: 3321 1392 3515
Fragments arranged by size:
3515 3321 1392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5723 5723
Size: 8228
XmaI C'CCGG_G
Cuts at: 663 4129 5248 663
Size: 3466 1119 3643
Fragments arranged by size:
3643 3466 1119
XmnI GAAnn'nnTTC
Cuts at: 570 602 1712 2262 3092 570
Size: 32 1110 550 830 5706
Fragments arranged by size:
5706 1110 830 550 32
ZraI GAC'GTC
Cuts at: 759 3861 759
Size: 3102 5126
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AscI AvrII BmgBI BmtI BsiWI BsrGI
BssHII BstZ17I Bsu36I CspCI FseI FspAI MfeI NdeI
NheI NotI PasI PmlI PpuMI PshAI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI