Restriction map for
pF1KA2001
(Circular) (Six-base) MAPSORT of: pF1KA2001.seq Check: 1831 from: 1 to: 4820
pF1KA2001 4820 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1746 1746
Size: 4820
AccI GT'mk_AC
Cuts at: 590 1858 590
Size: 1268 3552
Acc65I G'GTAC_C
Cuts at: 1836 1836
Size: 4820
AclI AA'CG_TT
Cuts at: 4660 4660
Size: 4820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 3077 3824 4092 2645
Size: 432 747 268 3373
Fragments arranged by size:
3373 747 432 268
AflIII A'CryG_T
Cuts at: 810 1535 2172 3276 4253 810
Size: 725 637 1104 977 1377
Fragments arranged by size:
1377 1104 977 725 637
AgeI A'CCGG_T
Cuts at: 3208 3208
Size: 4820
AlwNI CAG_nnn'CTG
Cuts at: 888 1438 2125 3692 888
Size: 550 687 1567 2016
Fragments arranged by size:
2016 1567 687 550
ApaI G_GGCC'C
Cuts at: 458 458
Size: 4820
ApaLI G'TGCA_C
Cuts at: 3590 3590
Size: 4820
ApoI r'AATT_y
Cuts at: 601 787 952 1805 1824 4173 4429 601
Size: 186 165 853 19 2349 256 992
Fragments arranged by size:
2349 992 853 256 186 165 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4820
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
AvaI C'yCGr_G
Cuts at: 214 346 652 1840 214
Size: 132 306 1188 3194
Fragments arranged by size:
3194 1188 306 132
BamHI G'GATC_C
Cuts at: 1428 1845 1428
Size: 417 4403
BanI G'GyrC_C
Cuts at: 543 658 1666 1836 2250 2498 2533 543
Size: 115 1008 170 414 248 35 2830
Fragments arranged by size:
2830 1008 414 248 170 115 35
BanII G_rGCy'C
Cuts at: 225 395 458 572 971 1834 2864 225
Size: 170 63 114 399 863 1030 2181
Fragments arranged by size:
2181 1030 863 399 170 114 63
BbeI G_GCGC'C
Cuts at: 662 2254 2502 662
Size: 1592 248 2980
Fragments arranged by size:
2980 1592 248
BbsI GAAGACnn'nnnn_
Cuts at: 1157 1746 1157
Size: 589 4231
BciVI GTATCCnnnnn_n'
Cuts at: 2718 3479 4820 2718
Size: 761 1341 2718
Fragments arranged by size:
2718 1341 761
BclI T'GATC_A
Cuts at: 2340 2340
Size: 4820
BglI GCCn_nnn'nGGC
Cuts at: 1664 1664
Size: 4820
BglII A'GATC_T
Cuts at: 2335 4372 2335
Size: 2037 2783
BlpI GC'TnA_GC
Cuts at: 1932 1932
Size: 4820
Bme1580I G_kGCm'C
Cuts at: 293 458 2445 2538 3594 293
Size: 165 1987 93 1056 1519
Fragments arranged by size:
1987 1519 1056 165 93
BmrI ACTGGGnnnn_n'
Cuts at: 580 720 2032 2113 2448 4592 580
Size: 140 1312 81 335 2144 808
Fragments arranged by size:
2144 1312 808 335 140 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 3187
Size: 4820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 243 571 1088 193
Size: 50 328 517 3925
Fragments arranged by size:
3925 517 328 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1996 2875 3367 3665 823
Size: 1173 879 492 298 1978
Fragments arranged by size:
1978 1173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 575 613 1438 4524 575
Size: 38 825 3086 871
Fragments arranged by size:
3086 871 825 38
BsaAI yAC'GTr
Cuts at: 811 2173 811
Size: 1362 3458
BsaBI GATnn'nnATC
Cuts at: 6 2334 4371 6
Size: 2328 2037 455
Fragments arranged by size:
2328 2037 455
BsaHI Gr'CG_yC
Cuts at: 118 659 1743 2251 2499 3201 4697 118
Size: 541 1084 508 248 702 1496 241
Fragments arranged by size:
1496 1084 702 541 508 248 241
BsaWI w'CCGG_w
Cuts at: 2009 2530 3208 3482 3629 4040 2009
Size: 521 678 274 147 411 2789
Fragments arranged by size:
2789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 572 602 572
Size: 30 4790
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 1252 1255 1258 1303 1306 1309 1336 1339
Size: 933 3 3 45 3 3 27 3
Cuts at: 1339 1342 1345 1366 1456 1477 1480 319
Size: 3 3 21 90 21 3 3659
Fragments arranged by size:
3659 933 90 45 27 21 21 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2245 3003 3580 4076 2245
Size: 758 577 496 2989
Fragments arranged by size:
2989 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2362 2408 3616 104
Size: 2258 46 1208 1308
Fragments arranged by size:
2258 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 971 1834 2612 2802 3594 971
Size: 863 778 190 792 2197
Fragments arranged by size:
2197 863 792 778 190
BsmI GAATG_Cn'
Cuts at: 198 198
Size: 4820
Bsp1286I G_dGCh'C
Cuts at: 225 293 395 458 572 971 1834 2445 2538
Size: 68 102 63 114 399 863 611 93
Cuts at: 2538 2612 2802 2864 3594 225
Size: 74 190 62 730 1451
Fragments arranged by size:
1451 863 730 611 399 190 114 102
93 74 68 63 62
BspEI T'CCGG_A
Cuts at: 4040 4040
Size: 4820
BspHI T'CATG_A
Cuts at: 3996 3996
Size: 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1870 2084 2386 2767 1870
Size: 214 302 381 3923
Fragments arranged by size:
3923 381 302 214
BsrBI CCG'CTC
Cuts at: 50 555 666 1062 3112 3166 4650 4815 50
Size: 505 111 396 2050 54 1484 165 55
Fragments arranged by size:
2050 1484 505 396 165 111 55 54
BsrDI GCAATG_nn'
Cuts at: 258 2732 258
Size: 2474 2346
BsrFI r'CCGG_y
Cuts at: 371 492 2818 2999 3208 371
Size: 121 2326 181 209 1983
Fragments arranged by size:
2326 1983 209 181 121
BssSI C'ACGA_G
Cuts at: 1796 3091 3449 1796
Size: 1295 358 3167
Fragments arranged by size:
3167 1295 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2197 2197
Size: 4820
BstBI TT'CG_AA
Cuts at: 1074 1737 3181 1074
Size: 663 1444 2713
Fragments arranged by size:
2713 1444 663
BstEII G'GTnAC_C
Cuts at: 723 1567 723
Size: 844 3976
BstXI CCAn_nnnn'nTGG
Cuts at: 1552 1552
Size: 4820
BstYI r'GATC_y
Cuts at: 1 1428 1845 2312 2335 2670 2916 3928 4014
Size: 1427 417 467 23 335 246 1012 86
Cuts at: 4014 4026 4372 1
Size: 12 346 449
Fragments arranged by size:
1427 1012 467 449 417 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2770 2951 3114 2770
Size: 181 163 4476
Fragments arranged by size:
4476 181 163
BtsI GCAGTG_nn'
Cuts at: 2063 2063
Size: 4820
DraI TTT'AAA
Cuts at: 1819 1819
Size: 4820
DrdI GACnn_nn'nnGTC
Cuts at: 2526 3384 2526
Size: 858 3962
EaeI y'GGCC_r
Cuts at: 227 274 989 1800 2405 2579 2970 2997 4342
Size: 47 715 811 605 174 391 27 1345
Cuts at: 4342 227
Size: 705
Fragments arranged by size:
1345 811 715 705 605 391 174 47
27
EagI C'GGCC_G
Cuts at: 2405 2405
Size: 4820
EarI CTCTTCn'nnn_
Cuts at: 1124 1274 1295 2843 3053 1124
Size: 150 21 1548 210 2891
Fragments arranged by size:
2891 1548 210 150 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 494 1750 3028 3338 3484 4630 494
Size: 1256 1278 310 146 1146 684
Fragments arranged by size:
1278 1256 1146 684 310 146
EcoICRI GAG'CTC
Cuts at: 969 1832 969
Size: 863 3957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 243 571 1088 2645 3077 3824 4092 193
Size: 50 328 517 1557 432 747 268 921
Fragments arranged by size:
1557 921 747 517 432 328 268 50
EcoNI CCTnn'n_nnAGG
Cuts at: 229 301 229
Size: 72 4748
EcoO109I rG'GnC_Cy
Cuts at: 1959 1959
Size: 4820
EcoRI G'AATT_C
Cuts at: 601 787 952 1805 1824 601
Size: 186 165 853 19 3597
Fragments arranged by size:
3597 853 186 165 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4820
FspI TGC'GCA
Cuts at: 2601 2601
Size: 4820
HaeII r_GCGC'y
Cuts at: 499 662 2254 2502 3524 4503 499
Size: 163 1592 248 1022 979 816
Fragments arranged by size:
1592 1022 979 816 248 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 452 572 604 2662 2692 2694 2724
Size: 32 32 120 32 2058 30 2 30
Cuts at: 2724 388
Size: 2484
Fragments arranged by size:
2484 2058 120 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 980 1859 980
Size: 879 3941
KasI G'GCGC_C
Cuts at: 658 2250 2498 658
Size: 1592 248 2980
Fragments arranged by size:
2980 1592 248
KpnI G_GTAC'C
Cuts at: 1840 1840
Size: 4820
MluI A'CGCG_T
Cuts at: 1535 4253 1535
Size: 2718 2102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 3491 3675 929
Size: 2562 184 2074
Fragments arranged by size:
2562 2074 184
MscI TGG'CCA
Cuts at: 276 2581 276
Size: 2305 2515
MslI CAynn'nnrTG
Cuts at: 2809 2936 2809
Size: 127 4693
MspA1I CmG'CkG
Cuts at: 272 460 549 1931 2245 2605 3618 3863 272
Size: 188 89 1382 314 360 1013 245 1229
Fragments arranged by size:
1382 1229 1013 360 314 245 188 89
NaeI GCC'GGC
Cuts at: 373 494 3001 373
Size: 121 2507 2192
Fragments arranged by size:
2507 2192 121
NarI GG'CG_CC
Cuts at: 659 2251 2499 659
Size: 1592 248 2980
Fragments arranged by size:
2980 1592 248
NgoMIV G'CCGG_C
Cuts at: 371 492 2999 371
Size: 121 2507 2192
Fragments arranged by size:
2507 2192 121
NspI r_CATG'y
Cuts at: 751 1873 2373 3280 751
Size: 1122 500 907 2291
Fragments arranged by size:
2291 1122 907 500
PciI A'CATG_T
Cuts at: 3276 3276
Size: 4820
PflMI CCAn_nnn'nTGG
Cuts at: 710 710
Size: 4820
PfoI T'CCnGG_A
Cuts at: 431 767 431
Size: 336 4484
PmeI GTTT'AAAC
Cuts at: 1819 1819
Size: 4820
PmlI CAC'GTG
Cuts at: 811 811
Size: 4820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 4788
PspOMI G'GGCC_C
Cuts at: 454 454
Size: 4820
PstI C_TGCA'G
Cuts at: 1867 2552 1867
Size: 685 4135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4820
PvuII CAG'CTG
Cuts at: 2245 2605 2245
Size: 360 4460
RsrII CG'GwC_CG
Cuts at: 3015 3015
Size: 4820
SacI G_AGCT'C
Cuts at: 971 1834 971
Size: 863 3957
SalI G'TCGA_C
Cuts at: 1857 1857
Size: 4820
SapI GCTCTTCn'nnn_
Cuts at: 2843 3053 2843
Size: 210 4610
SbfI CC_TGCA'GG
Cuts at: 1867 1867
Size: 4820
ScaI AGT'ACT
Cuts at: 1135 4337 1135
Size: 3202 1618
SfcI C'TryA_G
Cuts at: 33 1137 1683 1863 2548 3541 3732 33
Size: 1104 546 180 685 993 191 1121
Fragments arranged by size:
1121 1104 993 685 546 191 180
SfoI GGC'GCC
Cuts at: 660 2252 2500 660
Size: 1592 248 2980
Fragments arranged by size:
2980 1592 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
SgrAI Cr'CCGG_yG
Cuts at: 492 492
Size: 4820
SmaI CCC'GGG
Cuts at: 348 654 1842 348
Size: 306 1188 3326
Fragments arranged by size:
3326 1188 306
SmlI C'TyrA_G
Cuts at: 838 1975 2890 3382 3644 838
Size: 1137 915 492 262 2014
Fragments arranged by size:
2014 1137 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4820
SphI G_CATG'C
Cuts at: 751 1873 2373 751
Size: 1122 500 3198
Fragments arranged by size:
3198 1122 500
StuI AGG'CCT
Cuts at: 944 1658 944
Size: 714 4106
StyI C'CwwG_G
Cuts at: 704 1184 1954 2308 704
Size: 480 770 354 3216
Fragments arranged by size:
3216 770 480 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 711 742 1579 2403 2536 2995 3204 711
Size: 31 837 824 133 459 209 2327
Fragments arranged by size:
2327 837 824 459 209 133 31
TatI w'GTAC_w
Cuts at: 1133 4335 1133
Size: 3202 1618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1525 1557 4337 4369 1525
Size: 32 2780 32 1976
Fragments arranged by size:
2780 1976 32 32
Tth111I GACn'n_nGTC
Cuts at: 586 2617 586
Size: 2031 2789
XbaI T'CTAG_A
Cuts at: 1851 1851
Size: 4820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2315 2315
Size: 4820
XmaI C'CCGG_G
Cuts at: 346 652 1840 346
Size: 306 1188 3326
Fragments arranged by size:
3326 1188 306
XmnI GAAnn'nnTTC
Cuts at: 341 1658 341
Size: 1317 3503
ZraI GAC'GTC
Cuts at: 1744 1744
Size: 4820
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI